Uncovering the diuretic potential of Abutilon hirtum leaves extract in wistar albino rats
Abstract
Abutilon hirtum, traditionally recognized for its alleged diuretic effects, underwent examination to assess its viability as a natural substitute for synthetic diuretics. The methanolic extract of A. hirtum leaves (AHME) was methodically prepared using Soxhlet extraction technique and assessed for its diuretic activity in wistar albino rats. Five different groups were created from the animals: a control group, a standard group that received furosemide, and three test groups that received 150, 300 and 450 mg/kg of body weight of AHME. The evaluation of diuretic activity was conducted through the lipschitz test, which involved measuring various parameters including urine volume, pH, electrolyte excretion, diuretic effect and lipschitz value. The highest dose (450 mg/kg) substantially raised urine volume, electrolyte excretion, and pH compared to the control, with a Lipschitz value of 2.35. These results corroborate the traditional use of A. hirtum leaves and provide a basis to subsequent pharmacological research by confirming their diuretic ability.
Keywords:
Abutilon hirtum, folklore, methanol, lipschitz model, diuretic activityDOI
https://doi.org/10.25004/IJPSDR.2025.170105References
Wile D. Diuretics: a review. Annals of Clinical Biochemistry. 2012;49(5):419–31.Available from: doi: 10.1258/acb.2011.011281
Kehrenberg M, Bachmann H. Diuretics: a contemporary pharmacological classification Naunyn-Schmiedeberg’s. Archives of Pharmacology. 2022;395(6):619–27. Available from: doi: 10.1007/s00210-022-02247-0
Bell R, Mandalia R. Diuretics and the kidney. BJA Education. 2022;22(6):216-223. Available from: doi: 10.1016/j.bjae.2022.02.003.
Jha V, Padmaprakash K, Pandey R. Diuretic strategies in medical disorders. Current Medical Issues. 2018;16 (2):60-67. Available from: doi: 10.4103/cmi.cmi_15_18
Wright CI, Van-Buren L, Kroner CI, Koning MM. Herbal medicines as diuretics: a review of the scientific evidence. Journal of Ethnopharmacology. 2007:114 (1):1-31.Available from: doi: 10.1016/j.jep.2007.07.023.
Gomaa A. Samy M. Desoukey S. Kamel M, Pharmacognostical studies of leaf, stem, root and flower of Abutilon hirtum (Lam.) Sweet. International Journal of Pharmacognosy and Phytochemical Research. 2016; 8(1):199-216.
Hamed M, Laila Refahy L, Mohamed Abelaziz M. Assessing the bioactivity and antioxidative properties of some compounds isolated from Abutilon hirtum (Lam.). Asian Journal of Pharmaceutical and Clinical Research. 2017; 10 (3):333-34 Available from:
doi:10.22159/ajpcr.2017.v10i3.16229.
Qari S. Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays. Heliyon. 2023; 9:1-11. Available from: doi 10.1016/j.heliyon.2023.e18617
Gomaa A, Samy M, Desoukey S, Kamel M. Anti-inflammatory, analgesic, antipyretic and antidiabetic activities of Abutilon hirtum (Lam.) Sweet. Clinical Phytoscience. 2018; 4(11):1-7. Available from: doi:10.1186/s40816-018-0069-8.
Zhang Q, Lin G, Ye W. Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine 2018; 17:13-20. Available From: doi: 10.1186/s13020-018-0177-x.
Dubale S, Kebebe D, Zeynudin A, Abdissa N, Suleman S. Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. Journal of Experimental Pharmacology 2023; 8(15):51-62. Available from: doi 10.2147/JEP.S37980.
Khandelwal K, Sethi V. Preliminary phytochemical screening. Practical Pharmacognosy, 2014. 25.1-25.9
Welu G, Ebrahim M, Yimer, Hailu H. et.al., In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae). Evidence-Based Complementary and Alternative Medicine. 2020;1-9 Available from: doi: 10.1155/2020/1718708
Masood N, Jamil Q, Aslam M. et.al., Antioxidant, carbonic anhydrase inhibition and diuretic activity of Leptadenia pyrotechnica Forssk. Decne. Heliyon. 2023:1-13.Available from doi: 10.1016/j.heliyon.2023.e22485
Shanmuganathan P, Kumarappan M. Evaluation of diuretic, saluretic and natriuretic activity of hydrochlorothiazide in combination with misoprostol in wistar rats. National Journal of Physiology, Pharmacy and Pharmacolgy. 2018;8(8):1226-29. Available from: doi: 10.5455/njppp.2018.8.0518731052018.
Published
Abstract Display: 158 
PDF Downloads: 110 How to Cite
Issue
Section
Copyright (c) 2025 Vidya Vikram Nalawade, Pramodkumar Jaykumar Shirote
This work is licensed under a Creative Commons Attribution 4.0 International License.
