Phytochemical investigation and cytotoxic potential of leaf extract of Medinilla beddomei C B Clarke in breast cancer cell lines

Authors

  • Athira R K Nair Department of Botany and Centre for Research, St. Teresa’s College (Autonomous), Ernakulam, Kerala, India
  • Elsam Joseph Department of Botany and Centre for Research, St. Teresa’s College (Autonomous), Ernakulam, Kerala, India
  • Jinu John Department of Biotechnology, CMS college (Autonomous), Kottayam, Kerala, India

Abstract

Leaves of the plant Medinilla beddomei were extracted using Soxhlet and ultrasound-assisted extraction methods using polar solvents, acetone, and methanol to find out the extraction efficiency. The profiling of the phytoconstituents was done by LC-Qtof-MS analysis. An in vitro MTT study was used to test the antiproliferative property of M. beddomei acetone leaf extract in two breast cancer (MCF-7, MDA-MB-231) cells and a normal cell line (L929 cells). Cell migration was conducted by the scratch wound healing assay, and cell death was evaluated by the double staining method of ethidium bromide and acridine orange. The apoptotic potential of the leaf extract was examined in the cancer cell line and the observations were evaluated by flow cytometry. The findings of the studies show that ultrasound-assisted extraction using acetone as the solvent gave a better yield of phytochemicals. According to LC-Qtof-MS analysis, phytocompounds including phenolics, flavonoids, alkaloids, terpenoids, and glycosides are present in the leaf extracts. The acetone extract showed significant anticancer properties when tested against MCF-7 and MDA-MB-231 cell lines. MDA-MB-231 cells had an IC50 value of 68.27±0.83µg/ml, while MCF-7 cells had the lowest cell viability with an IC50 value of 46.19±1.58µg/ml. However, the plant extract showed negligible toxicity towards normal cells (IC50-386.12±4.07µg/ml). The acetone leaf extract of M. beddomei demonstrated a better antimetastatic effect in MCF-7 cells. Since this extract demonstrated apoptosis modulation at several stages of the MCF-7 cell cycle, it may be a promising target for medication research against breast cancer. The results of this investigation show that the acetone leaf extract of M. beddomei may have anticancer properties, and that ultrasonic extraction is a more effective method for extracting phytochemicals.

Keywords:

Apoptosis, Cytotoxicity, Medinilla beddomei, Ultra sound assisted extraction

DOI

https://doi.org/10.25004/IJPSDR.2025.170306

References

Nelson VK, Sahoo NK, Sahu M, Sudhan HH, Pullaiah CP, Muralikrishna KS. In vitro anticancer activity of Eclipta alba whole plant extract on colon cancer cell HCT-116. BMC Complementary Medicine and Therapies. 2020;20:1-8. Available from: https://doi.org/10.1186/s12906-020-03118-9

Al Monla R, Salma Y, Kouzayha A, Gali-Muhtasib H, Dassouki Z, Mawlawi H. Antioxidative, cytotoxic, and anti-metastatic potentials of Laurencia obtusa and Ulva lactuca seaweeds. Asian Pacific Journal of Tropical Biomedicine. 2021;11(7):308-316. Available from: https://doi.org/10.4103/2221-1691.317242

Khan MI, Bouyahya A, Hachlafi NE, Menyiy NE, Akram M, Sultana S, Zengin G, Ponomareva L, Shariati MA, Ojo OA, Dall’Acqua S. Anticancer properties of medicinal plants and their bioactive compounds against breast cancer: a review on recent investigations. Environmental Science and Pollution Research. 2022;29(17):24411-24444. Available from: https://doi.org/10.1007/s11356-021-17795-7

Rezai M, Davoodi A, Asori M, Azadbakht M. Cytotoxic activity of Citrullus colocynthis (L.) Schrad fruit extract on gastric adenocarcinoma and breast cancer cell lines. International Journal of Pharmaceutical Sciences Review and Research. 2017;45:175-178.

Anand P, Kunnumakara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B, Aggarwal BB. Cancer is a preventable disease that requires major lifestyle changes. Pharmaceutical Research. 2008;25(9):2097-2116. Available from: https://doi.org/10.1007/s11095-008-9661-9

Sharma A, Chakravarti B, Gupt MP, Siddiqui JA, Konwar R, Tripathi RP. Synthesis and anti breast cancer activity of biphenyl based chalcones. Bioorganic & Medical Chemistry. 2010;18(13):4711-4720. Available from: https://doi.org/10.1016/j.bmc.2010.05.015

Abdullaev FI. Plant-derived agents against cancer. Pharmacology and therapeutics in the new millennium. Narosa Publishing House, New Delhi. 2001.

Mongalo NI, Soyingbe OS, Makhafola TJ. Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae). Asian Pacific Journal of Tropical Biomedicine. 2019;9(7):307-314. Available from: https://doi.org/10.4103/2221-1691.261822

Al-Areer NW, Al Azzam KM, Al Omari RH, Al-Deeb I, Bekbayeva L, Negim E.S. Quantitative analysis of total phenolic and flavonoid compounds in different extracts from ginger plant (Zingiber officinale) and evaluation of their anticancer effect against colorectal cancer cell lines. Pharmacia. 2023;70(4):905-919. Available from: https://doi.org./10.3897/pharmacia.70.e103936

Agarwal G, Carcache PJ, Addo EM, Kinghorn AD. Current status and contemporary approaches to the discovery of antitumor agents from higher plants. Biotechnology advances. 2020;38:107337. Available from: https://doi.org/10.1016/j.biotechadv.2019.01.004

Avtanski D, Poretsky L. Phyto-polyphenols as potential inhibitors of breast cancer metastasis. Molecular Medicine. 2018;24:1-7. Available from: https://doi.org/10.1186/s10020-018-0032-7

Hanum AS, Prihastanti E, Jumari J. Ethnobotany of utilization, role, and philosophical meaning of parijoto (Medinilla spp) on Mount Muria in Kudus Regency, Central Java. In AIP Conference Proceedings. AIP Publishing, Indonesia, 2017, pp.1-6. Available from: https://doi.org/10.1063/1.4995210

Sasidharan N, Sujanapal P. The genus Medinilla Gaudich. ex DC. (Melastomataceae) in Peninsular India. RHEEDEA-KERALA-. 2005;15(2):103.

Shanavaskhan AE, Sivadasan M, Alfarhan AH, Thomas J. Ethnomedicinal aspects of angiospermic epiphytes and parasites of Kerala, India. Indian Journal of Traditional Knowledge. 2012;11(2): 250-258.

Pullaiah T, KrishnamurthyKV, Bahadur B. Ethnobotany of India: Western Ghats and West Coast of Peninsular India, Vol 2, Apple Academic Press, USA, 2016, pp.164.

Narayanankutty A, Sasidharan A, Job JT, Rajagopal R, Alfarhan A, Kim YO, Kim HJ. Mango ginger (Curcuma amada Roxb.) rhizome essential oils as source of environmental friendly biocides: Comparison of the chemical composition, antibacterial, insecticidal and larvicidal properties of essential oils extracted by different methods. Environmental Research. 2021.202:111718. Available from: https://doi.org/10.1016/j.envres.2021.111718

Ezez D, Tefera M. Effects of solvents on total phenolic content and antioxidant activity of ginger extracts. Journal of Chemistry. 2021;1:6635199. Available from: https://doi.org/10.1155/2021/6635199

Zhang QW, Lin LG. Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine. 2018;13:1-26. Available from: https://doi.org/10.1186/s13020-018-0177-x

Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry. 2003;51(3):609–614. Available from: https://doi.org/10.1021/jf020782a

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis. 2002;10(3):178-182. Available from: https://doi.org/10.38212/2224-6614.2748

Ghorai N, Chakraborty S, Gucchait S, Saha SK, Biswas S. Estimation of total Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent. Protocol Exchange. 2012;5(10):1038. Available from: https://doi.org/10.1038/protex.2012.055

Mohan MC, Abhimannue AP. Identification and characterization of Berberine in Tinospora cordifolia by liquid chromatography Quadrupole time of flight mass spectrometry (LC MS/MS q-tof) and evaluation of its anti inflammatory potential. Pharmacognosy Journal. 2017;9(3):350-355. Available from: http://dx.doi.org/10.5530/pj.2017.3.59

Arung ET, Wicaksono BD, Handoko YA, Kusuma IW, Yulia D, Sandra F. Anti-cancer properties of diethyl ether extract of wood from sukun (Artocarpus altilis) in human breast cancer (T47D) cells. Tropical Journal of Pharmaceutical Research. 2009;8(4):317-324. Available from: https://doi.org/10.4314/tjpr.v8i4.45223

Kandimalla R, Dash S, Kalita S, Choudhury B, Malampati S, Kalita K, Kotoky J. Bioactive guided fractions of Annona reticulata L. bark: protection against liver toxicity and inflammation through inhibiting oxidative stress and proinflammatory cytokines. Frontiers in pharmacology. 2016;22;7:168.

Bolla SR, Al-Subaie AM, Al-Jindan RY, Balakrishna JP, Ravi PK, Veeraraghavan VP, Pillai AA, Gollapalli SS, Joseph JP, Surapaneni KM. In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression. Heliyon. 2019;5(5). Available from: https://doi.org/10.1016/j.heliyon.2019.e01648

Zhang JH, Yu J, Li WX, Cheng CP. Inhibited Apoptosis in Rat Corpus Luteal Cells by Flow Cytometry and Fluorochromes. Chinese Journal of Physiology. 1998;41(2):121-126.

Somaida A, Tariq I, Ambreen G, Abdelsalam AM, Ayoub AM, Wojcik M, Dzoyem JP, Bakowsky U. Potent cytotoxicity of four Cameroonian plant extracts on different cancer cell lines. Pharmaceuticals. 2020;13(11):357. Available from: https://doi.org/10.3390/ph13110357

Joseph JM, Thomas B, Rajendran A, Prabhukumar KM. Medicinal chasmophytes of Urumbikkara hills, Idukki, Kerala, India. Asian Journal of Pharmaceutical Sciences. 2015;5(1):11-17.

Anusmitha KM, Aruna M, Job JT, Narayanankutty A, Benil PB, Rajagopal R, Alfarhan A, Barcelo D. Phytochemical analysis, antioxidant, anti-inflammatory, anti-genotoxic, and anticancer activities of different Ocimum plant extracts prepared by ultrasound-assisted method. Physiological and Molecular Plant Pathology. 2022;1(117):101746. Available from: https://doi.org/10.1016/j.pmpp.2021.101746

Kolap RM, Kakade PS, Gacche RN, Zimare SB. Assessment of Radical Scavenging Activity and Estimation of EC50 Values of Various Extracts of Leaves and Roots from Lobelia nicotianifolia Roth. (Wild Tobacco). Journal of Herbs, Spices & Medicinal Plants. 2021;27(4): 343-364. Available from: https://doi.org10.1080/10496475.2021.1932006

Ceci C, Lacal PM, Tentori L, De Martino MG, Miano R, Graziani G. Experimental evidence of the antitumor, antimetastatic and antiangiogenic activity of ellagic acid. Nutrients. 2018; 10(11):1756. Available from: https://doi.org/10.3390/nu10111756

Kim KH, Seo HS, Choi HS, Choi I, Shin YC, Ko SG. Induction of apoptotic cell death by ursolic acid through mitochondrial death pathway and extrinsic death receptor pathway in MDA-MB-231 cells. Archive of pharmacal research. 2011;34:1363-1372. Available from: https://doi.org/10.1007/s12272-011-0817-5

Dong W, Dong Q, Ding H. Ophiopogonin B induces reactive oxygen species-dependent apoptosis through the Hippo pathway in nasopharyngeal carcinoma. Molecular Medicine Reports. 2021;24(1):1-9. Available from: https://doi.org/10.3892/mmr.2021.12173

Hassabou NF, Farag AF. Anticancer effects induced by artichoke extract in oral squamous carcinoma cell lines. Journal of Egyptian National Cancer Institute. 2020;32:1-10. Available from:

https://doi.org/10.1186/s43046-020-00026-4

Kwan YP, Saito T, Ibrahim D, Al-Hassan FM, Ein Oon C, Chen Y, Jothy SL, Kanwar JR, Sasidharan S. Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction by Euphorbia hirta in MCF-7 breast cancer cells. Pharmaceutical Biology. 2016;54(7):1223-1236. Available from: https://doi.org/10.3109/13880209.2015.1064451

Published

30-05-2025
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“Phytochemical Investigation and Cytotoxic Potential of Leaf Extract of Medinilla Beddomei C B Clarke in Breast Cancer Cell Lines”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 17, no. 3, May 2025, pp. 260-8, https://doi.org/10.25004/IJPSDR.2025.170306.

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Research Article

How to Cite

“Phytochemical Investigation and Cytotoxic Potential of Leaf Extract of Medinilla Beddomei C B Clarke in Breast Cancer Cell Lines”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 17, no. 3, May 2025, pp. 260-8, https://doi.org/10.25004/IJPSDR.2025.170306.