Investigating Bioactive Compound Extraction and Evaluation of Xantolis tomentosa based on Response Surface Methodology
Abstract
Xantolis tomentosa (Roxb.) Raf. is an ethnomedicinal plant traditionally used for treating inflammatory and infectious disorders. In the current research, Response Surface Methodology (RSM) was used to optimize the extraction of bioactive compounds from X. tomentosa leaves. A Box–Behnken Design (BBD) was applied to evaluate the effects of solvent concentration (40–80%), extraction temperature (40–70°C), and extraction time (20–60 min) on phytochemical yield and biological activity. The optimized extraction conditions yielded elevated levels of total flavonoids (11.8 mg/g), total phenolics (0.98 mg/g), and total terpenoids (0.48 mg/g). The extract demonstrated strong antioxidant activity, with DPPH radical scavenging inhibition ≥ 83.2%, along with significant antimicrobial activity (maximum inhibition zone: 20.14 mm). GC–MS analysis identified multiple compounds with potential biological activity, supporting the observed antioxidant and antimicrobial properties. These findings demonstrate that statistically optimized extraction significantly enhances the bioactivity of X. tomentosa and provide scientific validation for its traditional medicinal applications.
Keywords:
Response Surface Methodology, Xantolis tomentosa, Phytochemicals, Antioxidant activity, biomedical propertyDOI
https://doi.org/10.25004/References
Chintada V, Golla N. Exploring the therapeutic potential of bioactive compounds from plant sources. In: Biotechnological Intervention in Production of Bioactive Compounds: Biosynthesis, Characterization and Applications. Springer Nature Switzerland; 2025:229–247. Available from: doi.org/10.1007/978-3-031-76859-0_15
Agrawal RS, Ranveer RC, Rathod NB, Nirmal NP. Phytochemicals as bioactive ingredients for functional foods. In: Recent Frontiers of Phytochemicals. Elsevier; 2023:95–108. Available from: doi.org/10.1016/B978-0-443-19143-5.00028-1
Kawatra A, Gupta S, Dhankhar R, Singh P, Gulati P. Application of phytochemicals in therapeutic, food, flavor, and cosmetic industries. In: Phytochemical Genomics: Plant Metabolomics and Medicinal Plant Genomics. Springer Nature Singapore; 2023:85–108. Available from: doi.org/10.1007/978-981-19-5779-6_4
Safe S, Jayaraman A, Chapkin RS, Howard M, Mohankumar K, Shrestha R. Flavonoids: structure–function and mechanisms of action and opportunities for drug development. Toxicology Research. 2021;37(2):147–162. Available from: doi.org/10.1007/s43188-020-00080-z
Bhattacharjee B, Sandhanam K, Ghose S, Barman D, Sahu RK. Market overview of herbal medicines for lifestyle diseases. In: Role of Herbal Medicines: Management of Lifestyle Diseases. Springer Nature Singapore; 2024:597–614. Available from: doi.org/10.1007/978-981-99-7703-1_30
Deshmukh RK, Gaikwad KK. Natural antimicrobial and antioxidant compounds for active food packaging applications. Biomass Conversion and Biorefinery. 2024;14(4):4419–4440. Available from: doi.org/10.1007/s13399-022-02623-w
Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, Valko M. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Archives of Toxicology. 2023;97(10):2499–2574. Available from: doi.org/10.1007/s00204-023-03562-9
Gnanasekaran G, Angelin Felicia E, Nithya SP, Arisdason W. Edible fruit-yielding wild plants of Tamil Nadu: a preliminary checklist. In: Pomotherapeutic Insights on Wild Edible Fruits. Springer Nature Singapore; 2025:305–326. Available from: doi.org/10.1007/978-981-96-0543-9_16
Adeleye OA, Femi-Oyewo MN, Bamiro OA, Bakre LG, Alabi A, Ashidi JS, Balogun-Agbaje OA, Hassan OM, Fakoya G. Ethnomedicinal herbs in African traditional medicine with potential activity for the prevention, treatment, and management of coronavirus disease 2019. Future Journal of Pharmaceutical Sciences. 2021;7(1):72. Available from: doi.org/10.1186/s43094-021-00223-5
de Oliveira AC, dos Anjos Cruz JM, Mar JM, Corrêa RF, da Silva Rodrigues JV, de Oliveira Mendes TA, Aguiar JPL, do Amaral Souza FDC, Neves KDOG, Santos ADC, Sanches EA. Abiu-do-Mato (Pouteria caimito, Sapotaceae): a promising Amazonian fruit with rich chemical composition, antioxidant potential, and cytotoxic safety. Food Research International. 2025;205:115984. Available from: doi.org/10.1016/j.foodres.2025.115984
Ghosh N, Pathak S, Malsawmdawngkimi, Kumar G, Gull A. Natural products and traditional herbal medicines as managerial therapies to combat rheumatoid arthritis. Clinical and Translational Medicine. 2024;22(1):2. Available from: doi.org/10.1007/s12018-024-09290-7
Divyalakshmi MV, Thoppil JE. Synthesis, characterization, antioxidant and antimicrobial activity of silver nanoparticles from ethnomedicinal yellow mangosteen leaf extract. Vegetos. 2025:1–12. Available from: doi.org/10.1007/s42535-025-01179-0
Bachhar V, Joshi V, Gangal A, Duseja M, Shukla RK. Identification of bioactive phytoconstituents, nutritional composition and antioxidant activity of Calyptocarpus vialis. Applied Biochemistry and Biotechnology. 2024;196(4):1921–1947. Available from: doi.org/10.1007/s12010-023-04640-5
Choudhary N, Chatterjee M, Kumar S, Singh G, Suttee A. Effect of conventional method and microwave assisted extraction on phytoconstituents of Chenopodium album. Materials Today: Proceedings. 2021;45:5362–5367. Available from: doi.org/10.1016/j.matpr.2021.01.947
Neethu S, Veena SK, Indulekha VC, Eapen J, Radhakrishnan KV. Phytoconstituents assessment and development of standardization protocol for ‘Nayopayam Kwatha’, a polyherbal Ayurvedic formulation. Journal of Ayurveda and Integrative Medicine. 2021;12(3):489–499. Available from: doi.org/10.1016/j.jaim.2021.05.002
Dunachie SJ, Day NP, Dolecek C. The challenges of estimating the human global burden of disease of antimicrobial resistant bacteria. Current Opinion in Microbiology. 2020;57:95–101. Available from: doi.org/10.1016/j.mib.2020.09.013
Carrique-Mas JJ, Choisy M, Van Cuong N, Thwaites G, Baker S. An estimation of total antimicrobial usage in humans and animals in Vietnam. Antimicrobial Resistance and Infection Control. 2020;9(1):16. Available from: doi.org/10.1186/s13756-019-0671-7
Wali AF, Jabnoun S, Razmpoor M, Najeeb F, Shalabi H, Akbar I. Account of some important edible medicinal plants and their socio-economic importance. In: Edible Plants in Health and Diseases: Cultural, Practical and Economic Value. Springer Nature Singapore; 2022:325–367. Available from: doi.org/10.1007/978-981-16-4880-9_14
Alemu M, Asfaw Z, Lulekal E, Warkineh B, Debella A, Sisay B, Debebe E. Ethnobotanical study of traditional medicinal plants used by the local people in Habru District, North Wollo Zone, Ethiopia. Journal of Ethnobiology and Ethnomedicine. 2024;20(1):4. Available from: doi.org/10.1186/s13002-023-00644-x
Xiang Y, Hou W, Wang M, Feng J, Li W, Liu Y. Secondary metabolites and antioxidant activity of different parts from the medicinal and edible Alpiniae oxyphylla Miq. Journal of Functional Foods. 2025;128:106803. Available from: doi.org/10.1016/j.jff.2025.106803
Sameeksha S, Puneet G, Ragini G. Exploring the bioactive-based therapeutic and culinary potential of Madhuca longifolia flower and delineating the research gaps in terms of scientific validation. Journal of Food Science and Technology. 2025;1–8. Available from: doi.org/10.1007/s13197-025-06348-2
Miranda JP, Cifuentes N, Bárcenas-Pérez D, Hájek J, Cheel J, Simirgiotis MJ, Sepúlveda B, Areche C. Green extraction of phenolics from Pouteria caimito using microwave-assisted techniques. Food Bioscience. 2025;66:106182. Available from: doi.org/10.1016/j.fbio.2025.106182
Khare N, Barot M, Singh S, Jain T. Network pharmacology reveals key targets and pathways of Madhuca longifolia for potential Alzheimer’s disease treatment. Cell Biochemistry and Biophysics. 2024;82(3):2727–2746. Available from: doi.org/10.1007/s12013-024-01389-4
Li Z, Duan Q, Mo Y, Xie F, Zhou Q, Sun P, Lu C, Xie F, Chen P. Effects of Alpinia oxyphylla fructus polysaccharide on the gelatinization, retrogradation, structural characteristics, antioxidant activity, and in vitro digestibility of corn starch. International Journal of Biological Macromolecules. 2025:143284. Available from: doi.org/10.1016/j.ijbiomac.2025.143284
Bachhar V, Joshi V, Gangal A, Duseja M, Shukla RK. In vitro antimicrobial, antidiabetic and anticancer activities of Calyptocarpus vialis extract and its integration with computational studies. ChemistrySelect. 2024;9(35):e202401414. Available from: doi.org/10.1002/slct.202401414
Singh SK, Patel S. Therapeutic phytoconstituents-I. In: Nanotechnology Based Delivery of Phytoconstituents and Cosmeceuticals. Springer Nature Singapore; 2024:39–61. Available from: doi.org/10.1007/978-981-99-5314-1_2
Guddi K, Sarkar A. Optimization of green extraction technologies for recovering bioactive compounds from Ixora coccinea waste flower biomass: a comparative response surface methodology and artificial neural network modeling. Sustainable Chemistry and Pharmacy. 2024;42:101830. Available from: doi.org/10.1016/j.scp.2024.101830
Mishra RR. Phytochemical analysis and comparison of some selected plants. Vegetos. 2024:1–12. Available from: doi.org/10.1007/s42535-024-01062-4
Nguyen HM, Huynh NTK, Ha LT, Pham TT. Utilizing X-ray fluorescence (XRF) method to evaluate the content of metal elements in soil and their effects on the total phenolic and flavonoid contents of some medicinal plants. Environmental Monitoring and Assessment. 2023;195(8):963. Available from: doi.org/10.1007/s10661-023-11585-z
Vo TP, Ho TAT, Ha NMH, Nguyen DQ. Optimization of ultrasonic and microwave-assisted extraction with natural deep eutectic solvents for enhanced recovery of phenolics and terpenoids from celery leaves. Biomass Conversion and Biorefinery. 2025;15(10):15053–15067. Available from: doi.org/10.1007/s13399-024-06246-1
Chatepa LEC, Mwamatope B, Chikowe I, Masamba KG. Effects of solvent extraction on the phytoconstituents and in vitro antioxidant activity properties of leaf extracts of the two selected medicinal plants from Malawi. BMC Complementary Medicine and Therapies. 2024;24(1):317. Available from: doi.org/10.1186/s12906-024-04619-7
Yakobi SH, Nwodo UU. Microbiological methodologies: comparative evaluation of microbial community and enhanced antibiotic susceptibility testing. Electronic Journal of Biotechnology. 2025. Available from: doi.org/10.1016/j.ejbt.2025.01.001
Zhu S, Miao Q, Chen Q, Tian L, Dong F, Guo Z, Li Q. Synthesis and antioxidant evaluation of coumarin-functionalised chitosan: a potent, non-toxic free radical scavenging compound. Carbohydrate Research. 2025;548:109311. Available from: doi.org/10.1016/j.carres.2024.109311
Ameur S, Toumi M, Bendif H, Derbak L, Yildiz I, Rebbas K, Demirtas I, Flamini G, Bruno M, Garzoli S. Cistus libanotis from Algeria: phytochemical analysis by GC/MS, HS-SPME-GC/MS, LC–MS/MS and its anticancer activity. Journal of Food Composition and Analysis. 2024;136:106747. Available from: doi.org/10.1016/j.jfca.2024.106747
Handayani DP, Kusuma HS, Timotius D, Sarastika T, Mahfud M, Darmokoesoemo H. Extraction of Centella asiatica herb using microwave-assisted solvent extraction: optimization of crude extract using Box-Behnken design. Green Analytical Chemistry. 2024;11:100146. Available from: doi.org/10.1016/j.greeac.2024.100146
Elsadek MF, Al-Numair KS. Profiling of phytochemical constituents of Terminalia chebula fruit extract by different solvent effects and synchronized analysis of FTIR and GCMS. Journal of King Saud University-Science. 2024;36(9):103414. Available from: doi.org/10.1016/j.jksus.2024.103414
Wanyo P, Chomnawang C, Huaisan K, Chamsai T. Comprehensive analysis of antioxidant and phenolic profiles of Thai medicinal plants for functional food and pharmaceutical development. Plant Foods for Human Nutrition. 2024;79(2):394–400. Available from: doi.org/10.1007/s11130-024-01179-6
Belew AA, Gebre SH. Comparative assessment of phenolic and flavonoid contents and antioxidant activities in methanol extracts of spices from Jigjiga market, Ethiopia. Pharmacological Research - Natural Products. 2025;6:100168. Available from: doi.org/10.1016/j.prenap.2025.100168
Krache I, Trabsa H, Boussoualim N, Baghiani A, Boublia A, Raish M, Benguerba Y. Comprehensive evaluation of antioxidant activity and xanthine oxidase inhibition by Lycium arabicum extracts: in vitro and in silico insights. South African Journal of Botany. 2025;187:306–315. Available from: doi.org/10.1016/j.sajb.2025.10.020
Ayodhya D, Ambala A, Balraj G, Kumar MP, Shyam P. Green synthesis of CeO2 nanoparticles using Manilkara zapota fruit peel extract for photocatalytic treatment of pollutants, antimicrobial, and antidiabetic activities. Results in Chemistry. 2022;4:100441. Available from: doi.org/10.1016/j.rechem.2022.100441
Purohit S, Bohra MK, Jain R. Identification of bioactive pentacyclic triterpenoids and fatty acid derivatives from Cissus quadrangularis and C. rotundifolia through untargeted metabolite profiling. Applied Biochemistry and Biotechnology. 2023;195(4):2235–2251. Available from: doi.org/10.1007/s12010-022-03940-6
de Sousa Ferreira M, Ribeiro FDO S, Dourado FF, de Jesus Oliveira AC, Araújo TDS, Brito LM, Pessoa C, de Lima LRM, de Paula RCM, Silva-Filho EC, da Silva DA. Production of galactan phthalates derivatives extracted from Gracilaria birdie: characterization, cytotoxic and antioxidant profile. International Journal of Biological Macromolecules. 2023;243:125254. Available from: doi.org/10.1016/j.ijbiomac.2023.125254
Zhang Y, Suo Y, Jiang Y, Ji Q, Fang R. Optimization of response surface methodology and in vitro antioxidant, antimicrobial activity of Phellodendron amurense callus tissue. Plant Cell, Tissue and Organ Culture. 2024;159(1):12. Available from: doi.org/10.1007/s11240-024-02872-9
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