SENSITIVE AND RAPID HPLC METHOD FOR THE DETERMINATION OF PIOGLITAZONE IN RAT SERUM

Authors

  • CH Ravikanth Drug Metabolism and Pharmacokinetics Lab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India
  • A Anil Kumar Drug Metabolism and Pharmacokinetics Lab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India
  • V Uday Kiran Drug Metabolism andab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India
  • S Prashanth Vaagdevi College of Pharmacy, Warangal, India
  • B Madhu Drug Metabolism and Pharmacokinetics Lab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India
  • Y Narsimha Reddy Drug Metabolism and Pharmacokinetics Lab, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India

Abstract

A sensitive, accurate and rapid high-performance liquid chromatography with UV-visible detection (HPLC-UV) method for the determination of Pioglitazone in rat serum has been developed. Rosiglitazone was used as internal standard. Pioglitazone and Rosiglitazone are extracted from serum using a liquid–liquid extraction procedure using ethyl acetate. Isocratic separation of Pioglitazone and Rosiglitazone is carried out using a reversed-phase phenomenex C18 (250 mm × 4.6 mm, 5µm) column with mobile phase consisting of methanol and 30 mM ammonium acetate buffer (pH adjusted to 5 with ortho-phosphoric acid) in the ratio 60:40 (v/v) and quantified by UV detection at 269 nm. Analytical run time was less than 10 min. Mean recovery was 97.12% for 0.1-10µg/ml concentrations. The assay exhibited good linear relationship. Quantification limit was at 50ng/ml of Pioglitazone and accuracy and precision were over the concentration range of 0.1-10µg/ml. This method can be used for routine clinical monitoring of Pioglitazone.

Keywords:

Ethyl acetate, HPLC, Pioglitazone, rat serum.

DOI

https://doi.org/10.25004/IJPSDR.2011.030109

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Published

01-01-2011
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How to Cite

“SENSITIVE AND RAPID HPLC METHOD FOR THE DETERMINATION OF PIOGLITAZONE IN RAT SERUM”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 1, Jan. 2011, pp. 38-41, https://doi.org/10.25004/IJPSDR.2011.030109.

Issue

Volume 3, Issue 1, 2011

Section

Research Article

How to Cite

“SENSITIVE AND RAPID HPLC METHOD FOR THE DETERMINATION OF PIOGLITAZONE IN RAT SERUM”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 1, Jan. 2011, pp. 38-41, https://doi.org/10.25004/IJPSDR.2011.030109.