PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF DRIED FRUITS OF HELICTERES ISORA IN DEXTRAN SULFATE SODIUM (DSS) INDUCED ULCERATIVE COLITIS IN EXPERIMENTAL WISTAR RATS

Authors

  • Prajakta Murudkar Department of Pharmacology, AISSMS College of Pharmacy, Savitribai Phule Pune University, Pune-411001, Maharashtra, India
  • Swati Kolhe Department of Pharmacology, AISSMS College of Pharmacy, Savitribai Phule Pune University, Pune-411001, Maharashtra, India
  • Sachin Tembhurne Department of Pharmacology, AISSMS College of Pharmacy, Savitribai Phule Pune University, Pune-411001, Maharashtra, India

Abstract

Ulcerative colitis is a most common form of inflammatory bowel disease (IBD) which mainly affect colon. The treatment of ulcerative colitis depends upon severity of the diseases. The aim of the present study was to determine the effect of hydroalcoholic extract of dried fruits of Helicteres isora in dextran sulfate sodium induced ulcerative colitis in experimental wistar rats. In this study wistar rats of either sex were divided into five experimental groups, where control group recived only distilled water. Group 2 was negative control group which received 4% dextran sulfate sodium (DSS) from drinking water between 15th to 21st day. Group 3 received low dose of hydroalcholic extract of Helicteres isora (HI) at a dose 100mg/kg orally along with 4% DSS from drinking water between from drinking water between 15th to 21st day. Group 4 received high dose of hydroalcholic extract of Helicteres isora (HI) at a dose 200mg/kg orally along with 4% DSS from drinking water between from drinking water between 15th to 21st day. In group 5 sulfasalazine was used as a standard drug at a dose 100mg/kg orally along with 4% DSS from drinking water between from drinking water between 15th to 21st day. Twenty four hours after treatment animals were sacrificed and further macroscopical, biochemical, histopathological evaluation was done and all the results were compare with control at p<0.05 significant value.

Keywords:

Ulcerative Colitis, mucosal inflammation, dextran sulfate sodium, Helicteres isora

DOI

https://doi.org/10.25004/IJPSDR.2020.120404

References

Ordás I, Eckmann L, Talamini M, Baumgart DC, Sandborn WJ. Ulcerative colitis. Lancet. 2012;380(9853):1606-1619.

Adams SM, Bornemann PH. Ulcerative colitis. Am Fam Physician. 2013;87(10):699-705.

Whaley KG, Rosen MJ. Contemporary medical management of acute severe ulcerative colitis. Inflamm Bowel Dis. 2019;25(1):56-66.

Shen ZH, Zhu CX, Quan YS, et al. Relationship between intestinal microbiota and ulcerative colitis: mechanisms and clinical application of probiotics and fecal microbiota transplantation. World J Gastroenterol. 2018;24(1):5-14.

Eichele DD, Kharbanda KK. Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World Journal of Gastroenterology. 2017 Sep 7;23(33):6016.

Ke F, Yadav PK, Ju LZ. Herbal medicine in the treatment of ulcerative colitis. Saudi journal of gastroenterology: official journal of the Saudi Gastroenterology Association. 2012 Jan;18(1):3.

Kumar N, Singh AK. Plant profile, phytochemistry and pharmacology of Avartani (Helicteres isora Linn.): A review. Asian Pacific Journal of Tropical Biomedicine. 2014 May 1;4:S22-26.

Muthukumar M, Kumar TS, Rao MV. Phenology and seed germination of the Indian Screw Tree Helicteres isora L.(Malvales: Malvaceae). Journal of Threatened Taxa. 2017 Dec 26;9(12):11040-11044.

Al-Rejaie SS, Abuohashish HM, Al-Enazi MM, Al-Assaf AH, Parmar MY, Ahmed MM. Protective effect of naringenin on acetic acidinduced ulcerative colitis in rats. World Journal of Gastroenterology: WJG. 2013 Sep 14;19(34):5633.

Froushani SM. The Beneficial effects of hypiran in ameliorating rat model of ulcerative colitis. Zahedan Journal of Research in Medical Sciences. 2018 Jan;20(1).

Poojari S, Bhargavi R, Mohan M. Protective effect of ethanolic extract of seeds of Coriandrum sativum l. in acetic acid-induced ulcerative colitis in male Wistar rats. Journal of Integrated Health Sciences. 2013 Jul 1;1(2):95.

Nehring, Sara M, et al. “C Reactive Protein (CRP).” StatPearls, StatPearls Publishing, 2020.

Ghosh S, Shand A, Ferguson A. Ulcerative colitis. BMJ. 2000;320: 1119.

Gajendran M, Loganathan P, Jimenez G, Catinella AP, Ng N, Umapathy C, et al. A comprehensive review and update on ulcerative colitis. Disease-a-month. 2019 Mar 2.

Love BL. Management of patients with inflammatory bowel disease: current and future treatments. Acute Pain. 2019;10:00.

Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N. Oxidative stress and inflammation: what polyphenols can do for us?. Oxidative Medicine and Cellular Longevity. 2016;2016.

DeRoche TC, Xiao SY, Liu X. Histological evaluation in ulcerative colitis. Gastroenterology Report. 2014 Aug 1;2(3):178-192.

Published

30-07-2020
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How to Cite

“PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF DRIED FRUITS OF HELICTERES ISORA IN DEXTRAN SULFATE SODIUM (DSS) INDUCED ULCERATIVE COLITIS IN EXPERIMENTAL WISTAR RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 12, no. 4, July 2020, pp. 325-30, https://doi.org/10.25004/IJPSDR.2020.120404.

Issue

Volume 12, Issue 4, 2020

Section

Research Article

How to Cite

“PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF DRIED FRUITS OF HELICTERES ISORA IN DEXTRAN SULFATE SODIUM (DSS) INDUCED ULCERATIVE COLITIS IN EXPERIMENTAL WISTAR RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 12, no. 4, July 2020, pp. 325-30, https://doi.org/10.25004/IJPSDR.2020.120404.