Evaluation of in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits
Abstract
In this research work to evaluate in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits. The chemical fingerprinting was carried out by high performance thin layer chromatography. It was carried out by the CAMAG HPTLC system equipped with Linomat V sample applicator, twin through plate development chamber, TLC scanner III and integration software WIN CATS-4.02. Physalis peruviana fruit extract was tested for phytochemical screening and in vitro anti-oxidant enzymes like 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH), total antioxidant activity and reducing ability. Physalis peruviana fruit extract effectively scavenged free radicals at all different concentrations and showed its potent antioxidant activity. Phytochemical analysis revealed the presence of various major phytoconstituents like alkaloids, flavonoids, saponins, phenolics, tannins and anthraquinones. The HPTLC fingerprint qualitatively revealed predominant amount of quercetin. Physalis peruviana fruit extract will be subjected to further extensive studies to isolate and identify their active constituents which are useful for understanding their mechanism of action as antioxidants.
Keywords:
HPTLC, Antioxidant, DPPH, Physalis peruvianaDOI
https://doi.org/10.25004/IJPSDR.2020.120411References
Randhir R, Lin YT, Shetty K. Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Process Biochem. 2004;39:637-646.
Siggurder Ingvarsson. Cancer introduction. Cancer Biology. 2001;11:323-325.
Alugoju Phaniendra, Dinesh Babu Jestadi, Latha Periyasamy. Free Radicals: Properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem. 2015;30(1):11-26.
Kovacic P, Jacintho JD. Mechanisms of carcinogenesis: Focus on oxidative stress and electron transfer. Curr Med Chem. 2001;8(7):773-796.
Block g, Jensen DJ, Morrow JD, Holland N, Norkus EP, Milne gL, et al. Vitamin C treatment reduces elevated C-reactive protein. Free Radic Biol Med. 2009;46(1):70-77.
Licodiedoff S, Kozlowski LAD, Ribani RH. Flavonoid rates of gosseberry fruits Physalis peruviana determined by HPLC through the optimization and validation of the analytic method. Int J Food Sci Nutr Eng. 2013;3(1):1-6.
Wu SJ, Nq LT, Huang YM, Lin DL, Wang SS, Huang SN, et al. Antioxidant activities of Physalis peruviana. Biol Pharm Bull. 2005;28(6):963-966.
Zavala D, Mauricio Q, Pelayo A, Posso M, Rojas J, Wolach V. Cytotoxic effect of Physalis peruviana (capuli) in colon cancer and chronic myeloid leukemia. An Fac Med Lima. 2006;67(4):283-289.
Lan Q, Zhnag L, Shen M, Jo WJ, Vermeulen R, Li g, et al. Large scale evaluation of candidate genes identifies association between DNA repair and genomic maintenance and development of benzene hematotoxicity. Carcinogenesis. 2009;30(1):50-58.
Wu SJ, Chang SP, Lin DL, Wang SS, Hou FF, Ng LT. Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells, Food Chem Toxicol. 2009;47(6):1132-1138.
Vega galvez A, Zura BL, Lemus MR, Martinez MJ, Quispe FI, Puente L, et al. Influence of drying temperature on dietary fibre, rehydration properties, texture and microstructure of cape gooseberry (Physalis peruviana L.). Jour Food Sci and Tech. 2013;52(4):2304-2311.
Sirwaiker AR, Rajendran K, Punitha ISR. In vitro antioxidant studies on the benzyl tetra isoquinone alkaloid. Biol. Pharm. Bull. 2006;29(9):2079-2080.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 1958;181:1199-1200.
Rice Evan CA, Burdon RH. Free radical damage and its control. Elsevier Science BV Netherlands. 1994;28:125.
Rao MS, Raman MV. Biochemical Systematics and Ecology. 2004;32:447-448.
Hausteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharm. 1983;32:1141-1148.
Cao g, Sofic E, Prior RL. Antioxidant and pro-oxidative behavior of flavonoids: Structure activity relationships. Free Radical. Biol. Med. 1997;22:749-760.
Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinia grandis L. leaf extract. Afr J Tradit Complemen. Altern Med. 2007;5(1):61-73.
Soares JR, Dinis TC, Cunha AP, Almedia LM. Antioxidant activities of some extracts of Thymus zygis. Free Radic Res. 1997;26(5):469-478.
Fraisse A, Temmam S, Deboosere N, Guillier L, Delobel A, Maris P, et al. Comparison of chlorine and peroxyacetic-based disinfectant to inactivate Feline calcivirus, murine nonovirus and hepatitis A virus on lettuce. Int J Food Microbiol. 2011;151(1):98-104.
Chithambara MKN, Vanitha A, Mahadeva SM, Ravishankar GA. Antioxidant and antimicrobial activity of Cissus quadrangularis L. J Med Food. 2003;6(2):99-105.
Prieto P, Pineda M, Aquilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337-341.
Yazdanparast R, Bahramikia S, Ardestani A. Nasturtium oficinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact. 2008;172(3):176-184.
Frankel N, German JB. Antioxidants in foods and health: Problems and fallacies in the field. J Sci Food Agric. 2006;86(13):1999-2001.
Sharififar F, dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem. 2009;112:885-888.
Prasad KN, Divakar S, Shivamurthy GR, Aradhya SM. Isolation of a free radical-scavenging antioxidant from water spinach (Ipomoea aquatica Forsk). J Sci Food Agri. 2005;85(9):1461-1468.
Jadhav SJ, Nimbalkar SS, Kulkarni AD, Madhavi DL. Food antioxidants technological: toxicological and health perspectives. 1st ed. CRC Press. New York: Dekker; 1996. p. 5-63.
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