IN VITRO DEVELOPMENT OF CALLUS FROM NODE OF MIMUSOPS ELENGI - AS SUBSTITUTE OF NATURAL BARK
Abstract
In this study, results of in vitro callus development from Mimusops elengi as substitute of bark were formulated. By different auxine/cytokinine ratio from young nodal explant compact, globular and fragile types of callus were developed. Methanolic extract of all types of callus and bark were studied for extractable matter, antibacterial activity, phytochemical profiling, biouautography and Thin Layer Chromatography (TLC) banding patterns. Results of all calluses were compared with results of natural bark of M. elengi. TLC pattern of three types of callus and bark revealed similar banding pattern, and same bioactivity was also observed in bioautography. Our results indicate that natural bark & in vitro developed callus of M. elengi posses similar chemical profiling and bioactivity regardless the type & colour of callus.
Keywords:
Bioactivity, Mimusops elengi, antibacterial activity, bioautographyDOI
https://doi.org/10.25004/IJPSDR.2010.020412References
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5. Srinualchai P, Tejasen P. Investigation of the diuretic actions of Mimusops elengi (Pikhun) in dogs and rats. Chieng Mai Med. Bull 1974; 13 (1): 5-21.
6. Singh VP, Sharma SK, Khare VS. Medicinal plants from Ujjain district Madhya Pradesh- Part-II. Indian Drugs Pharm. Ind 1980; 5: 7-12.
7. Kusumoto IT, Nakabayashi T, Kida H, et al. Screening of various plant extracts used in Ayurvedic medicine for inhibitory effects on Human immunodeficiency virus type 1 (HIV-1) protease. Phytother. Res 1995; 9:180-184.
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9. Hong TD, Ellis RH. A Protocol to determine seed storage behavior. IPGRI Technical Bulletin No. 1. International Plant Genetic Resources Institute, Rome, 64, 1996.
10. Truong MH, Tran DH, Nguyen TH, et al. Seed development, maturation and storage behavior of Mimusops elengi L. New Forest 2006; 32: 9 –19.
11. Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plantarum 1962; 15: 473-497.
12. Pelczar MJ, Chan ECS, Krieg NR. Microbiology, fifth ed. TATA Mc graw Hill Publishing Com.Ltd. New Delhi, 1993.
13. Perez C, Paulin M, Bazerque P. An antibiotic assay by the well agar method. Acta. Biological Medicine Experitimentalis 1990; 15: 113-115.
14. Slusarenko AJ, Longland AC, Whitehead JM. Convenient, sensitive and rapid assay for antibacterial activity of phytoalexins. Botanica Heavetica 1998; 99(2): 203-207.
15. Harbone JB. Phytochemical Methods – A Guide to Modern Techniques of plant analysis 2nd edition, Chapman and Hall London, New York, 1984.
16. Biondi S, Thorpe TA. Clonal propagation of forest tree species In: A.N.Rao (Eds), Procceding of the COSTED symposium on “Tissue Culture of economically important plants”. COSTED and Asian Network of Biological Sciences, Singapore 1982; 197-204.
17. Murashige T. Plant propagation through tissue cultures. Ann. Rev. Plant Physiol 1974; 25: 135-166.
18. Ahuja MR. Biotechnology in forest trees. Plant Research Development 1991; 33:106-120.
19. Bhojwani SS, Razdan MK. Development in crop Sciences (5) Plant Tissue Culture: Theory and Practice. Elsevier Science Publishers, Amsterdam, 1983.
20. Torrey J. The initiation of organized development in plants. Adv. Morphog 1966; 5: 39-91.
21. Mathysse AG, Torrey JG. Nutritional requirement for polyploid mitosis in cultured pea root segments. Physiol. Plant 1967b; 20: 661-672.
22. Skoog F, Miller C. Chemical regulation of growth and organ formation in plant tissue cultures. In vitro Symp. Exp. Bio 1957; 11: 118-131.
23. Hilderbrandt AC, Willium JC, Johns WH, Riker A. Growth of edible chlorophyllous plant tissue in vitro. Am .J. Bot 1963; 50: 248-254.
24. Ripley KP, Preece JE. Micropropagation of Euphorbia lathyrus L. Plant Cell Tissue and Organ Culture 1986; 7: 213-218.
25. Ho RK, Kamlesh RP, Trevor AT. Regeneration of mulberry plantlets through tissue culture. Bot. Gaz 1985; 146 (3): 335-340.
26. Olila D, Olwa O, Opuda AJ. Antibacterial and antifungal activities of extracts of Zanthoxylum chalybeum and Warburgia ugandensis, Ugandan medicinal plants African Health Sciences 2001; 1(2): 66-72.
27. Fridous AJ, Islam SNLM, Faruque ABM. Antimicrobial activity of the leaves of Adhatoda vasica, Calotropis gigantean, Nerium odorum and Ocimum sanctum. Bangladesh J. Bot 1990; 227.
28. Singh K, Sudarshana MS. Antimicrobial activity of Baliospermum axillare plant and callus extract. Asian J.Microbiol. biotechnol. Environ. Sci 2003; 5: 571-574.
29. Marcos JS, Paulo SP, Suzelei CF, Regina CC, Izabel YI, Diones AD. Comparative study of antibacterial and antifungal activity of callus culture and adult plants extracts from Alternanthera maritime (Amaranthaceae). Braz. J. of Micro 2003; 34: 131-136.
30. Hamid E, Jaber SM, Mahmoud MEO. Antimicrobial and cytotoxic activity of the Extracts of Khat callus cultures. In J.Janick (Eds), In Perspectives on new crops and new uses. ASHS Press, Alexandria, 1999.
31. Nayeemulla S, Sudarshana MS, Umesha S, Hariprasad P. Antimicrobial activity of Rauvolfia tetraphylla and Physalis minima leaf and callus extracts. A. J. of biotechnology 2006; 5(10): 946-950.
32. Misra G, Mitra CR. Constituents of bark of Mimusops elengi. Phytochemistry 1967b; 6: 1909.
33. Natesh S. The changing scenario of herbal drug: Role of botanists, Phytomorphology Golden Jubilee Issue 2001; 75-95.
34. Ahmed I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethanopharmacology 2001; 74: 113-123.
35. Betina V. Bioautography in paper and thin layer chromatography and its scope in the antibiotic field. J. Chromatography 1973; 78: 41-51.
2. Hooker JD. Flora of British India, Vol.3 London: Reeve & Co, 1882.
3. Reddy MB, Reddy KR, Reddy MN. A Survey of plant crude drugs of Anantapur district, Andhra Pradesh, India. Int. J. Crude Drug Res 1989; 27 (3): 141-155.
4. Mokkhasmit M, Ngarmwathana W, Sawasadimongkol K, Permphiphat U. Pharmacological evaluation of Thai Medicinal Plants (contd.). J. Med. Ass. Thailand 1971; 54 (7): 490-504.
5. Srinualchai P, Tejasen P. Investigation of the diuretic actions of Mimusops elengi (Pikhun) in dogs and rats. Chieng Mai Med. Bull 1974; 13 (1): 5-21.
6. Singh VP, Sharma SK, Khare VS. Medicinal plants from Ujjain district Madhya Pradesh- Part-II. Indian Drugs Pharm. Ind 1980; 5: 7-12.
7. Kusumoto IT, Nakabayashi T, Kida H, et al. Screening of various plant extracts used in Ayurvedic medicine for inhibitory effects on Human immunodeficiency virus type 1 (HIV-1) protease. Phytother. Res 1995; 9:180-184.
8. Chunekar KC, Pandey GS. Bhavaprakasa Nighantu, Varanasi: Chaukhambha Bharati Academy, 2002.
9. Hong TD, Ellis RH. A Protocol to determine seed storage behavior. IPGRI Technical Bulletin No. 1. International Plant Genetic Resources Institute, Rome, 64, 1996.
10. Truong MH, Tran DH, Nguyen TH, et al. Seed development, maturation and storage behavior of Mimusops elengi L. New Forest 2006; 32: 9 –19.
11. Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plantarum 1962; 15: 473-497.
12. Pelczar MJ, Chan ECS, Krieg NR. Microbiology, fifth ed. TATA Mc graw Hill Publishing Com.Ltd. New Delhi, 1993.
13. Perez C, Paulin M, Bazerque P. An antibiotic assay by the well agar method. Acta. Biological Medicine Experitimentalis 1990; 15: 113-115.
14. Slusarenko AJ, Longland AC, Whitehead JM. Convenient, sensitive and rapid assay for antibacterial activity of phytoalexins. Botanica Heavetica 1998; 99(2): 203-207.
15. Harbone JB. Phytochemical Methods – A Guide to Modern Techniques of plant analysis 2nd edition, Chapman and Hall London, New York, 1984.
16. Biondi S, Thorpe TA. Clonal propagation of forest tree species In: A.N.Rao (Eds), Procceding of the COSTED symposium on “Tissue Culture of economically important plants”. COSTED and Asian Network of Biological Sciences, Singapore 1982; 197-204.
17. Murashige T. Plant propagation through tissue cultures. Ann. Rev. Plant Physiol 1974; 25: 135-166.
18. Ahuja MR. Biotechnology in forest trees. Plant Research Development 1991; 33:106-120.
19. Bhojwani SS, Razdan MK. Development in crop Sciences (5) Plant Tissue Culture: Theory and Practice. Elsevier Science Publishers, Amsterdam, 1983.
20. Torrey J. The initiation of organized development in plants. Adv. Morphog 1966; 5: 39-91.
21. Mathysse AG, Torrey JG. Nutritional requirement for polyploid mitosis in cultured pea root segments. Physiol. Plant 1967b; 20: 661-672.
22. Skoog F, Miller C. Chemical regulation of growth and organ formation in plant tissue cultures. In vitro Symp. Exp. Bio 1957; 11: 118-131.
23. Hilderbrandt AC, Willium JC, Johns WH, Riker A. Growth of edible chlorophyllous plant tissue in vitro. Am .J. Bot 1963; 50: 248-254.
24. Ripley KP, Preece JE. Micropropagation of Euphorbia lathyrus L. Plant Cell Tissue and Organ Culture 1986; 7: 213-218.
25. Ho RK, Kamlesh RP, Trevor AT. Regeneration of mulberry plantlets through tissue culture. Bot. Gaz 1985; 146 (3): 335-340.
26. Olila D, Olwa O, Opuda AJ. Antibacterial and antifungal activities of extracts of Zanthoxylum chalybeum and Warburgia ugandensis, Ugandan medicinal plants African Health Sciences 2001; 1(2): 66-72.
27. Fridous AJ, Islam SNLM, Faruque ABM. Antimicrobial activity of the leaves of Adhatoda vasica, Calotropis gigantean, Nerium odorum and Ocimum sanctum. Bangladesh J. Bot 1990; 227.
28. Singh K, Sudarshana MS. Antimicrobial activity of Baliospermum axillare plant and callus extract. Asian J.Microbiol. biotechnol. Environ. Sci 2003; 5: 571-574.
29. Marcos JS, Paulo SP, Suzelei CF, Regina CC, Izabel YI, Diones AD. Comparative study of antibacterial and antifungal activity of callus culture and adult plants extracts from Alternanthera maritime (Amaranthaceae). Braz. J. of Micro 2003; 34: 131-136.
30. Hamid E, Jaber SM, Mahmoud MEO. Antimicrobial and cytotoxic activity of the Extracts of Khat callus cultures. In J.Janick (Eds), In Perspectives on new crops and new uses. ASHS Press, Alexandria, 1999.
31. Nayeemulla S, Sudarshana MS, Umesha S, Hariprasad P. Antimicrobial activity of Rauvolfia tetraphylla and Physalis minima leaf and callus extracts. A. J. of biotechnology 2006; 5(10): 946-950.
32. Misra G, Mitra CR. Constituents of bark of Mimusops elengi. Phytochemistry 1967b; 6: 1909.
33. Natesh S. The changing scenario of herbal drug: Role of botanists, Phytomorphology Golden Jubilee Issue 2001; 75-95.
34. Ahmed I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethanopharmacology 2001; 74: 113-123.
35. Betina V. Bioautography in paper and thin layer chromatography and its scope in the antibiotic field. J. Chromatography 1973; 78: 41-51.
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01-10-2010
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“IN VITRO DEVELOPMENT OF CALLUS FROM NODE OF MIMUSOPS ELENGI - AS SUBSTITUTE OF NATURAL BARK”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 2, no. 4, Oct. 2010, pp. 281-5, https://doi.org/10.25004/IJPSDR.2010.020412.
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How to Cite
“IN VITRO DEVELOPMENT OF CALLUS FROM NODE OF MIMUSOPS ELENGI - AS SUBSTITUTE OF NATURAL BARK”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 2, no. 4, Oct. 2010, pp. 281-5, https://doi.org/10.25004/IJPSDR.2010.020412.
