PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF POLYHERBAL HYDROETHANOLIC EXTRACT IN STREPTOZOTOCIN INDUCED Β-CELL DESTRUCTION IN RATS
Abstract
In the view of pathogenicity of diabetics, there are certain factors involved to produce oxidative stress over the pancreatic -cells, that may be due glucose toxicity or due to involvement of free radical mechanisms. So, possible protection of -cells against oxidative stress can be achieved by employing antioxidants rich herbs, which will not only acts as free radical scavengers and also for prevention of -cells from further damage in Streptozotocin induced Type 1 diabetic rats. Preliminary phytochemical investigation of the polyherbal hydroethanolic extract (PHHE) supports the presence of active constituents like alkaloids, glycosides, flavonoids, terpenoids, etc for whose the anti oxidant property was assessed by -carotene linoleate in vitro method of oxidation in TLC plate. To ascertain the antioxidant property in vivo, the enzyme level studies like SOD, CAT & GSH in liver and pancreas were made and also the deeper investigation of role of anti oxidants and possible protection over pancreatic -cells were observed.
Keywords:
Antioxidants, Type 1 Diabetes, Hydroethanolic, Polyherbal, StreptozotocinDOI
https://doi.org/10.25004/IJPSDR.2012.040105References
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8. Yin MC, Cheng WS. Antioxidant activity of several Allium members. J Agric Food Chem. 1998; 46: 4097-4101.
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11. Harbone JB. Phyto chemical methods. Edn 2, Chapman and Hall, London, 1984.
12. Ramachandran S, Anbu J, Saravanan M, Gnanasam SK, Sridhar K. Antioxidant and anti-inflammatory properties of Citrus sinensis peel extract. Ind J Pharm Sci. 2002; 64, 1; 66-68.
13. Soon YY, Tan BKH. Evaluation of Hypoglycemic and Antioxidant activities of Morinda officinalis in STZ induced diabetic rats. Singapore Med J. 2002; 43, 2; 077-085.
14. Uchiyma M, Mihara M. Determination of Malondialdehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978; 86: 271-278.
15. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47: 469-474.
16. Aebi H. Catalase invitro In: Packer L, Editor. Methods in Enzymology. San Diego: Academic Press Inc. 1984; 105: 121-126.
17. Sedlak J, Lindsay RHL. Estimation of total and non-protein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem. 1968; 25: 192-205.
18. Mukherjee KI. Medical Laboratory Technology, Edn 1. Tata McGraw Hill Publications, New Delhi. 1989: pp 124.
19. Pietta PG. Flavonoids as antioxidants. J Nat Prod. 2000; 63: 1035-1042.
20. Bolkents S, Yanardag R, Tabako A, Ozsoy Sacan O. Effects of chard “Beta vulgaris L. var. cicla” extract on pancreatic β cells in streptozotocin induced diabetic rats. A morphological and biochemical study. J Ethpharmacol. 2000; 73: 251-9.
21. Ferber S, Halkin A, Cohan H, Ber I, Einav Y, Goldberg I. Pancreatic and duodenum homeobox gene-I induces expression of insulin gene in liver and ameliorates streptozotocin induced hyperglycemia. Nat Med. 2000; 6: 568-72.
22. Saravanan S, Pari L. Antihyperlipidemic and antiperoxidative effect of Diasulin, a polyherbal formulation in alloxan induced hyperglycemic rats. BMC Com Alt Med 2005; 5: 1-8.
23. Tushar K Bera, Deasis De, Kausik Chatterjee, Kazi M Ali, Debidas Ghosh. Effect of Diahsis, a polyherbal formulation, in streptozotocin induced diabetic male albino rats. Inter J Ayur Res. 2010; 1: 1, 18-24.
24. Zhang XF, Tan BKH. Antihyperglycemic and antioxidant properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Pharmacol Physiol. 2000; 27: 358-63.
2. Esterbauer H, Cheesemann KH. Lipid peroxidation: part II pathological implications. Chem Phys Lipids. 1987; 45: 103-367.
3. Halliwell B. How to characterize a biological antioxidant. Free Rad Res Commun. 1990; 9: 1-32.
4. Halliwell B. Antioxidants: Basics. Advances in Pharmacology. Edn 38, London, Academic press. 1997, pp. 3-5.
5. Hideaki Kaeto, Yoshitaka Kalimoto, Jun-ichiro Miyagawa. Beneficial effects of Antioxidants in Diabetes. Diabetes. 1999; 48: 2398-2406.
6. Robert D Hoeldtke, Kimberly D Bryner, Daniel R Mc Neill. Oxidative stress and insulin requirements in patients with recent-onset type 1 diabetes. The J of Clin Endcrlogy Mtblsm. 2005; 88, 4: 1624-1628.
7. Ashok K Tiwari, Madhusudana Rao J. Diabetes mellitus and multiple therapeutic approaches of phytochemical: Present status and future prospects. Curt sci. 2002; 83, 1: 30-38.
8. Yin MC, Cheng WS. Antioxidant activity of several Allium members. J Agric Food Chem. 1998; 46: 4097-4101.
9. Kamble SM, Kamlakkar PL, Vaidya S. Assessment of antioxidant potentials of coccinia indica in human diabetes. Ind Med Gaz. 1998; 132, 8: 221-223.
10. Suckling KE, Jackson B. Animal models of human lipid metabolism. Pro Lipid Res. 1993; 32: 1-24.
11. Harbone JB. Phyto chemical methods. Edn 2, Chapman and Hall, London, 1984.
12. Ramachandran S, Anbu J, Saravanan M, Gnanasam SK, Sridhar K. Antioxidant and anti-inflammatory properties of Citrus sinensis peel extract. Ind J Pharm Sci. 2002; 64, 1; 66-68.
13. Soon YY, Tan BKH. Evaluation of Hypoglycemic and Antioxidant activities of Morinda officinalis in STZ induced diabetic rats. Singapore Med J. 2002; 43, 2; 077-085.
14. Uchiyma M, Mihara M. Determination of Malondialdehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978; 86: 271-278.
15. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47: 469-474.
16. Aebi H. Catalase invitro In: Packer L, Editor. Methods in Enzymology. San Diego: Academic Press Inc. 1984; 105: 121-126.
17. Sedlak J, Lindsay RHL. Estimation of total and non-protein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem. 1968; 25: 192-205.
18. Mukherjee KI. Medical Laboratory Technology, Edn 1. Tata McGraw Hill Publications, New Delhi. 1989: pp 124.
19. Pietta PG. Flavonoids as antioxidants. J Nat Prod. 2000; 63: 1035-1042.
20. Bolkents S, Yanardag R, Tabako A, Ozsoy Sacan O. Effects of chard “Beta vulgaris L. var. cicla” extract on pancreatic β cells in streptozotocin induced diabetic rats. A morphological and biochemical study. J Ethpharmacol. 2000; 73: 251-9.
21. Ferber S, Halkin A, Cohan H, Ber I, Einav Y, Goldberg I. Pancreatic and duodenum homeobox gene-I induces expression of insulin gene in liver and ameliorates streptozotocin induced hyperglycemia. Nat Med. 2000; 6: 568-72.
22. Saravanan S, Pari L. Antihyperlipidemic and antiperoxidative effect of Diasulin, a polyherbal formulation in alloxan induced hyperglycemic rats. BMC Com Alt Med 2005; 5: 1-8.
23. Tushar K Bera, Deasis De, Kausik Chatterjee, Kazi M Ali, Debidas Ghosh. Effect of Diahsis, a polyherbal formulation, in streptozotocin induced diabetic male albino rats. Inter J Ayur Res. 2010; 1: 1, 18-24.
24. Zhang XF, Tan BKH. Antihyperglycemic and antioxidant properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Pharmacol Physiol. 2000; 27: 358-63.
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01-09-2018
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“PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF POLYHERBAL HYDROETHANOLIC EXTRACT IN STREPTOZOTOCIN INDUCED Β-CELL DESTRUCTION IN RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 1, Sept. 2018, pp. 25-30, https://doi.org/10.25004/IJPSDR.2012.040105.
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How to Cite
“PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF POLYHERBAL HYDROETHANOLIC EXTRACT IN STREPTOZOTOCIN INDUCED Β-CELL DESTRUCTION IN RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 1, Sept. 2018, pp. 25-30, https://doi.org/10.25004/IJPSDR.2012.040105.
