EFFECT OF GLYMIN, A POLYHERBAL FORMULATION ON LIPID PROFILE AND HISTOPATHOLOGICAL EXAMINATION IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
Abstract
Diabetes Mellitus is a chronic widely spread human disease. Streptozotocin-induced diabetes in rats had been shown to be associated with functional and morphological changes in liver and kidney. This study was undertaken to investigate the effect of glymin on lipid profile and histopathological study of liver and kidney in streptozotocin-induced diabetic rats. Lipid profiles were investigated in serum and tissues (liver and kidney) of normal and streptozotocin-induced diabetic rats treated with glymin (50 and 100 mg/kg/day) for the period of 35 days. Diabetic rats exhibited an increase in the levels of total cholesterol, triglycerides, free fatty acids, phospholipids and low density lipoprotein cholesterol (LDL-cholesterol), and a decrease in the level of high density lipoprotein cholesterol (HDL-cholesterol). The administration of glymin shows decreased levels of total cholesterol, triglycerides, free fatty acids, phospholipids and LDL-cholesterol, and an increased level of HDL-cholesterol. The findings of this study indicate that the administration of glymin resulted in a better lipid profile which helps to recover the pathological effects of diabetes on liver and kidney of streptozotocin-induced diabetic rats.
Keywords:
Diabetes mellitus, Streptozotocin, Glymin, Histopathology, Lipid profileDOI
https://doi.org/10.25004/IJPSDR.2012.040109References
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27. Palumbo PJ. Metformin: effect on cardiovascular risk factor in patients with non-insulin dependent diabetes mellitus. J Diabetes Complicat. 1998; 12:110-109.
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29. Sailaja YR, Baskar R, Srinivas Rao CS, Saralakumari D. Membrane lipids and protein-bound carbohydrates status during the maturation of reticulocytes to erythrocytes in type 2 diabetes. Clin Chim Acta. 2004; 341:185-192.
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31. Lewis GF, Steiner G. Hypertriglyceridemia and its metabolic consequences as a risk factor for atherosclerotic cardiovascular disease in non-insulin dependent diabetes mellitus. Diab Metab Rev. 1996; 12:37-56.
32. Loh KC, Thai AC, Lui KF, Ng WY. High prevalence of dyslipidaemia despite adequate glycaemic control in patients with diabetes. Ann Acad Med Sing. 1996; 25:228-232.
33. Steiner G, Stewart D, Hosking JD. Baseline characteristics of the study population in the Diabetes Atherosclerosis Intervention Study (DAIS). World Health Organization Collaborating Centre for the Study of Atherosclerosis in Diabetes. Am J Cardiol. 1999; 84:1004-1010.
2. Taskinen MR. Diabetic dyslipidemia. Atherosclerosis Supplements. 2002; 3:47-51.
3. Miller CJ, Dunn EV, Hashim IB. Glycemic index of 3 varieties of dates. Saudi Med J. 2002; 23(5):536-538.
4. Sharma S, Kulkarni SK, Chopra K. Curcumin, the active principle of turmeric (Curcuma longa), ameliorates diabetic nephropathy in rats. Clin Exp Pharmacol Physiol. 2006; 33(10):940-945.
5. Law MR. Lowering heart disease risk with cholesterol reduction: evidence from observational studies and clinical trials. Eur Heart J. 1999; 1:3-8.
6. Posner BM, Cobb JL, Belanger AJ, Cupples LA, D’Agostino RB, Stokes J. Dietary lipid predictors of coronary heart disease in men. The Framingham Study. Arch Intern Med. 1991; 151:1181-1187.
7. Castelli WP, Anderson K, Wilson PW, Levy D. Lipid risk of coronary heart disease: The Framingham Study. Ann Epidemiol. 1992; 2:23-28.
8. Bailey CJ, Day C. Traditional treatments for diabetes. Diab Care. 1989; 12:553-564.
9. Mitra SK, Gopumadhavan S, Muralidhar TS, Anturlikar SD, Sujatha MB. Effect of a herbomineral preparation D-400 in streptozotocin induced diabetic rats. J Ethnopharmacol. 1996; 54:41-46.
10. Annapurna A, Kanaka R, Mahalakshmi D, Murali Krishna K. Antidiabetic activity of a polyherbal preparation (tincture of punchparna) in normal and diabetic rats. Indian J Exp Biol. 2001; 39:500-502.
11. Bhattacharya SK, Satyan KS, Chakrbarti A. Effect of Trasina, an Ayurvedic herbal formulation, on pancreatic islet superoxide dismutase activity in hyperglycaemic rats. Indian J Exp Biol. 1997; 35:297-299.
12. The WHO Expert Committee on Diabetes Mellitus. Technical Report Series 646, Geneva, and World Health Organisation 1980.
13. Jia W, Gao WY, Xiao PG. Antidaibetic drugs of plant origin used in China: Composition, pharmacology and hypoglycemic mechanisms. Zhongguo Zhong Yao Za Zhi. 2003; 28:108-113.
14. Elder C. Ayurveda for diabetes mellitus: a review of the biomedical literature. Altern Ther Health Med. 2004; 10:44-50.
15. Pari L, Ramakrishnan R, Venkateswaran S. Antihyperglycaemic effect of Diamed, an herbal formulation in experimental diabetes in rats. J Pharm Pharmacol. 2001; 53:1139-1143.
16. Pari L, Saravanan G: Antidiabetic effect of coagent db, a herbal drug in alloxan induced diabetes mellitus. Comp Biochem Physiol. Part C. 2002; 131:19-25.
17. Subash Babu P, Prince PSM. Antihyperglycemic and antioxidant effect of hyponidd an ayurvedic herbomineral formulation in streptozotocin induced diabetic rats. J Pharm Pharmacol. 2004; 56:1435-1442.
18. Folch J, Less M, Solane SGH. A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem. 1957; 226:497-509.
19. Zlatkis A, Zak B, Bogle GH. A method for determination of serum cholesterol. J Clin Med. 1953; 41:486-492.
20. Falholt K, Falholt NM, Lund B. An easy colorimetric method for routine determination of free fatty acids in plasma. Clin Chim Acta. 1973; 46:105-111.
21. Zilversmit DB, Davis AK. Micro determination of phospholipids by TCA precipitation. J Lab Clin Med. 1950; 35:155-161.
22. Assmann G. A fully enzymatic colorimetric determination of HDL-cholesterol in the serum. Internist. 1979; 20:559-567.
23. Schettler G, Nussel E. Method for triglycerides. Aeb Med Soz Med Prav Med. 1975; 10:25.
24. Steinberger J, Moorehead C, Katch V, Rocchini AP. Relationship between insulin resistance and abnormal lipid profile in obese adolescents. J Pediatr. 1995; 126:690-695.
25. Sadur CN, Yost TJ, Eckel RH. Insulin responsiveness of adipose tissue lipoprotein lipase is delayed but preserved in obesity. J Clin Endocrinol Metab. 1984; 59:1176-1182.
26. Wang L, Zhang XT, Zhang HY, Yao HY, Zhang H. Effect of Vaccinium bracteatum Thunb. leaves extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice. J Ethnopharmacol. 2010; 130:465-469.
27. Palumbo PJ. Metformin: effect on cardiovascular risk factor in patients with non-insulin dependent diabetes mellitus. J Diabetes Complicat. 1998; 12:110-109.
28. Arvind K, Pradeep R, Deepa R, Mohan V. Diabetes and coronary artery diseases. Indian J Med Res. 2002; 116:163-176.
29. Sailaja YR, Baskar R, Srinivas Rao CS, Saralakumari D. Membrane lipids and protein-bound carbohydrates status during the maturation of reticulocytes to erythrocytes in type 2 diabetes. Clin Chim Acta. 2004; 341:185-192.
30. Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoe bilimli leaf extract on blood glucose and lipids in streptozotocin diabetic rats. J Ethnopharmacol. 2000; 72:69-76.
31. Lewis GF, Steiner G. Hypertriglyceridemia and its metabolic consequences as a risk factor for atherosclerotic cardiovascular disease in non-insulin dependent diabetes mellitus. Diab Metab Rev. 1996; 12:37-56.
32. Loh KC, Thai AC, Lui KF, Ng WY. High prevalence of dyslipidaemia despite adequate glycaemic control in patients with diabetes. Ann Acad Med Sing. 1996; 25:228-232.
33. Steiner G, Stewart D, Hosking JD. Baseline characteristics of the study population in the Diabetes Atherosclerosis Intervention Study (DAIS). World Health Organization Collaborating Centre for the Study of Atherosclerosis in Diabetes. Am J Cardiol. 1999; 84:1004-1010.
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01-01-2012
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“EFFECT OF GLYMIN, A POLYHERBAL FORMULATION ON LIPID PROFILE AND HISTOPATHOLOGICAL EXAMINATION IN STREPTOZOTOCIN-INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 1, Jan. 2012, pp. 49-55, https://doi.org/10.25004/IJPSDR.2012.040109.
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How to Cite
“EFFECT OF GLYMIN, A POLYHERBAL FORMULATION ON LIPID PROFILE AND HISTOPATHOLOGICAL EXAMINATION IN STREPTOZOTOCIN-INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 1, Jan. 2012, pp. 49-55, https://doi.org/10.25004/IJPSDR.2012.040109.
