PHYTOCHEMICAL ANALYSIS & IN-VITRO ANTI OBESITY ACTIVITY OF DIFFERENT FRACTIONS OF METHANOLIC EXTRACT OF FAGONIA CRETICA L.

Authors

  • Divyang Patel Department of Pharmacognosy, Institute of Pharmacy, Nirma University, S.G. Highway, Ahmedabad - 382481, Gujarat, India
  • Vimal Kumar ITM School of Pharmacy, ITM (SLS) Baroda University, Dhanora Tank Road, Village-Paldi, Nr. Jarod, Tal- Waghodia, Vadodara - 391510, Gujarat, India

Abstract

Obesity is one of the most prevalent health concerns among all age groups & populations worldwide, resulting into a significant increase in mortality and morbidity related to metabolic disorders. Targeting one or more enzymes involved in lipid metabolism can be selective for evaluation of anti obesity action of drug. The present study was aimed to evaluate in vitro anti obesity action by inhibiting pancreatic lipase & ᾳ amylase enzyme by various fractions of methanolic extract of aerial parts Fagonia cretica L. along with their phytochemical analysis. The n- hexane (HFFC), Chloroform (CFFC), Ethyl acetate (EAFFC), n-butanol (BFFC) & aqueous fractions (AQFFC) were prepared from methanolic extracts of F. cretica L & were analyzed for qualitative as well as quantitative phytochemical study using reported methods. The qualitative phytochemical studies of prepared extract & fractions showed presence of flavonoids, saponins, phenolics, alkaloids & carbohydrates. All the fractions were then examined for their in-vitro lipase inhibitory & ᾳ amylase inhibitory activities at a concentration level of 50, 100, 150 & 200µg/ml and their percentage inhibitory effects were reported. Among the analyzed samples, BFFC showed highest lipase inhibitory action i.e. 83.02 ± 2.47% as compared to other fractions. EAFFC showed significantly higher ᾳ amylase inhibitory action i.e. 80.22 ± 1.18% as compared to other fractions.

Keywords:

Obesity, Fagonia cretica L, Pancreatic lipase, ᾳ amylase

DOI

https://doi.org/10.25004/IJPSDR.2020.120311

References

1. Hu FB, Li TY, Colditz GA, Willett WC, Manson JE. Television watching and other sedentary behaviors in relation to risk of obesity and type 2 diabetes mellitus in women. J of American Med Assoc. 2003; 289:1785-1791.
2. Verger R. Interfacial activation of lipases: facts and antifacts. Trends in Biosciences. 1997; 15:32–37.
3. Trivedi PC. Medicinal Plants Utilization & Conservation. Edn 2, Vol.1, Aavishkar Publication, Jaipur, 2009, pp 260.
4. Baquar SR. Medicinal and Poisonous Plants of Pakistan. Printas. Karachi; 1989, pp.198-199.
5. Meyer BN, Ferrrigni NR, Putnam JE. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982; 45:31-34.
6. Hussain I, Ullah R, Ullah R, Khurram M, Ullah N, Baseer A, Khan FA, Khattak MR, Zahoor M, Khan J, Khan N. Phytochemical analysis of selected medicinal plants. Afr J Biotech. 2011; 10:7487–7492.
7. Hussain A, Zia M, Mirza B. Cytotoxic and antitumor potential of Fagonia cretica L. Turk J Biol. 2007; 31:19-24.
8. Lam M, Carmichael AR, Griffiths HR. An aqueous extract of Fagonia cretica induces DNA damage, cell cycle arrest and apoptosis in breast cancer cells via FOXO3a and p53 expression. Plos one. 2012; 7:6.
9. Sharma S, Joseph L, George M, Gupta V. Analgesic and antimicrobial activity of Fagonia indica. Pharmacology online. 2009; 3:623-632.
10. Anjum MI, Ahmed E, Jabbar A, Malik A, Ashraf M, Moazzam M. Antimicrobial constituents from Fagonia cretica. J of Chem Soc of Pakistan. 2007; 6:634-639.
11. Abhirami V, Khosa RL, Dhar SK, Sahai M. Investigation on Fagonia cretica-Its effect on hormonal profile and immunomodulation in rats. Ancient Science of Life. 1996; 15:4: 259- 263.
12. Eldin HS, Gadir HA, Hassan AW. Evaluation of the hepatoprotective activity of Fagonia cretica L, Res. & Rew: J of Pharmacog & Phytochem. 2015; 3:1-6.
13. Ali SS, Kasoju N, Luthra A, Singh A, Hallihosur S, Shahu A. Indian Medicinal plants as source of antioxidants, Food Res.Int. 2008; 41:1-15.
14. Ayurvedic Pharmacopoeia of India. Part I, Edn 1, Vol. 5, Ministry of Health and Family Welfare, Department of Health, New Delhi, 2001, pp.30.
15. Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. Chapman and Hall: London; 1998, pp.34-86.
16. Trease GE, Evans WC. Pharmacognosy. Edn 11. London: Brailliar Tiridel Can Macmillan Publishers; 1989, pp.60–75.
17. Shah BN, Nayak BS. Experimental Pharmacognosy. Edn 1. S.Vikas & Company, Jalandhar. 2008, pp.190-200.
18. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64:555-559.
19. Obadoni B, Ochuko P. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in edo and delta states of Nigeria. Global J of Pure & Applied Sci. 2002; 8:203-208.
20. Etoundi CB, Kuaté D, Ngondi JL, Oben J. Anti-amylase, anti-lipase and antioxidant effects of aqueous extracts of some Cameroonian spices. J of Nat Prod. 2010; 3:165-171.
21. Xiao Z, Storms R, Tsang A. A quantitative starch-iodine method for measuring alpha amylase and glucoamylase activities. Anal Biochem. 2006, 351:146-148.
22. Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology. Edn 5. New Delhi: Elsevier, 2003, pp.410-419.

Published

30-05-2020
Statistics
Abstract Display: 883
PDF Downloads: 648
Dimension Badge

How to Cite

“PHYTOCHEMICAL ANALYSIS & IN-VITRO ANTI OBESITY ACTIVITY OF DIFFERENT FRACTIONS OF METHANOLIC EXTRACT OF FAGONIA CRETICA L”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 12, no. 3, May 2020, pp. 282-6, https://doi.org/10.25004/IJPSDR.2020.120311.

Issue

Volume 12, Issue 3, 2020

Section

Research Article

How to Cite

“PHYTOCHEMICAL ANALYSIS & IN-VITRO ANTI OBESITY ACTIVITY OF DIFFERENT FRACTIONS OF METHANOLIC EXTRACT OF FAGONIA CRETICA L”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 12, no. 3, May 2020, pp. 282-6, https://doi.org/10.25004/IJPSDR.2020.120311.