SCREENING OF ISOLATED FRACTIONS OF DENDROPHTHOE FALCATA METHANOL STEM EXTRACT FOR ITS EFFECTS ON REPRODUCTIVE FUNCTIONS OF MALE RATS
Abstract
In our previous study methanol crude extract of Dendrophthoe falcata Ettingsh (family-Loranthaceae), stem showed contraceptive like activities in male albino rats. In the present study proven fertile male rats were treated with different isolated fractions (Chloroform: Methanol) of D. falcata stem at 50 mg/rat/day for 60 days. On day 61th the animals were autopsied, testes and accessory sex organs i.e. epididymides, seminal vesicle, ventral prostate were dissected out and weighed. Sperm dynamics and serum testosterone level were assessed; also testicular histological studies were done. The sperm motility and density were significantly reduced in all the treatment groups. Testosterone levels were declined significantly. Evaluations of testicular cell dynamics and histological studies suggest disrupt or inhibited spermatogenesis.
Keywords:
Dendrophthoe falcata, testosterone, testicular cell dynamics, spermatogenesisDOI
https://doi.org/10.25004/IJPSDR.2012.040116References
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15. Malini T, Manimaran RR, Arunakaran J, Aruldhas MM, Govinda RP. Effects of piperine on testes of albino rats. J Ethnopharmacol. 1999; 64:219-225.
16. Lohiya NK, Ansari AS. Male contraceptive agents. In: Joy KR, Krishna A, Halder C (Eds). Comparative endocrinology and Reproduction. Narosa Publishing House, New Delhi, 1999, pp. 260-277.
17. Sharma N, Jacob D. Antifertility investigation and toxicological screening of the petroleum ether extract of the leaves of Mentha arvensis L. in male albino rats. J Ethnopharmacol. 2001; 75:5-12.
18. Sarkar SN, Majumdar AC, Chattopadhyay SK. Effect of isoproturon on male reproductive system: Clinical, histological and histoenzymological studies in rats. Ind J Exp Biol.1997; 35:133-138.
19. Carballada R, Esponda P. Role of fluid from seminal vesicle and coagulating glands in sperm transport into the uterus and fertility in rats. J Reprod Fertile. 1992; 95:639-648.
20. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl. 2004; 6:259-268.
21. Dufau M, Veldheis J, Fraiali F, Johnson M, Catt K. Mode of bioactive LH secretion in man. J Clin Endo Meta. 1983; 57:993.
22. Hall P, Charpannier C, Nakamura M, Gabbioni G. Role of microfilaments in response of Leydig cell to LH. J St Biochem. 1979; 11:1361.
2. Gupta RS, Kachhawa JBS. Evaluation of contraceptive activity of methanol extract of Dendrophthoe falcata stem in male albino rats. J Ethnopharmacol. 2007; 112:215-218.
3. Singh VK, Ali ZA. A contribution to the ethnopharmacological study of the Udaipur forests of Rajasthan, India. Fitoterapia. 1992; 63:136-144.
4. Alkutti NA, Srinivasan KK, Gundu RP, Udupa AC, Keshavamurthy KR. Diuretic and antilithiatic activity of Dendrophthoe falcata. Fitoterapia 1993; 64:325-331.
5. Bhakuni DS, Goyal AK, Jain S, Mehrotra BN, Patnaik GK, Prakash V. Screening of Indian plants for biological activity: Part XIII. Ind J Exp Biol. 1998; 26:883-904.
6. Nair AGR, Krishnakumary P. Flavonoids of Dendrophthoe falcata Ettingsh growing on different host plants. Ind J Chem. 1990; 29B:584-585.
7. Prasad MRN, Chinoy NJ, Kadam KM. Changes in succinate dehydrogenase levels in the rat’s epididymis under normal and altered physiological conditions. Fertil Steril. 1972; 23:186-190.
8. Be’langer A, Caron S, Picard V. Simultaneous radio-immuno assay of progestins, androgens and estrogens in rat testis. J Steroid Biochem. 1980; 13(2):185-190.
9. Mazaro R, Stasi LCD, Filho SAV, Kempinas WDG. Decrease in sperm number after treatment of rats with Austroplenckia populnea. Contraception 2000; 62:45-50.
10. Piyachaturawat P, Timinkul A, Chuncharunee A, Suksamaarn A. Effect of Curcuma extract on male fertility in rats. Pharm Biol. 1999; 37(1):22-27.
11. Sherines RJ, Howard SS. Male infertility. In: Harrison JH, Gitles RF, Pearlmutter AD, Stamy TA, Walsh PC, eds Campbell’s Urology. 4th ed. Philadelphia W B. Saunders. 1978; 715.
12. D’souza UJA, Narayana K. Induction of seminiferous tubular atrophy by single dose of 5- flurourosil (5-FU) in wistar rats. Ind J Physiol Pharmacol. 2001; 45:87-94.
13. VijayKumar B, Sangamma I, SharanaBasappa A, Patil SB. Antispermatogenic and hormonal effects of Crotalaria juncea Linn. seed extracts in male mice. Asian J Androl. 2004; 6:67-70.
14. Joshi AR, Ahmed RN, Pathan KM, Manivannan B. Effects of Azadirachta indica on testes and its recovery in albino rats. Ind J Exp Biol. 1996; 34:1091-1094.
15. Malini T, Manimaran RR, Arunakaran J, Aruldhas MM, Govinda RP. Effects of piperine on testes of albino rats. J Ethnopharmacol. 1999; 64:219-225.
16. Lohiya NK, Ansari AS. Male contraceptive agents. In: Joy KR, Krishna A, Halder C (Eds). Comparative endocrinology and Reproduction. Narosa Publishing House, New Delhi, 1999, pp. 260-277.
17. Sharma N, Jacob D. Antifertility investigation and toxicological screening of the petroleum ether extract of the leaves of Mentha arvensis L. in male albino rats. J Ethnopharmacol. 2001; 75:5-12.
18. Sarkar SN, Majumdar AC, Chattopadhyay SK. Effect of isoproturon on male reproductive system: Clinical, histological and histoenzymological studies in rats. Ind J Exp Biol.1997; 35:133-138.
19. Carballada R, Esponda P. Role of fluid from seminal vesicle and coagulating glands in sperm transport into the uterus and fertility in rats. J Reprod Fertile. 1992; 95:639-648.
20. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl. 2004; 6:259-268.
21. Dufau M, Veldheis J, Fraiali F, Johnson M, Catt K. Mode of bioactive LH secretion in man. J Clin Endo Meta. 1983; 57:993.
22. Hall P, Charpannier C, Nakamura M, Gabbioni G. Role of microfilaments in response of Leydig cell to LH. J St Biochem. 1979; 11:1361.
Published
01-01-2012
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“SCREENING OF ISOLATED FRACTIONS OF DENDROPHTHOE FALCATA METHANOL STEM EXTRACT FOR ITS EFFECTS ON REPRODUCTIVE FUNCTIONS OF MALE RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 1, Jan. 2012, pp. 84-87, https://doi.org/10.25004/IJPSDR.2012.040116.
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How to Cite
“SCREENING OF ISOLATED FRACTIONS OF DENDROPHTHOE FALCATA METHANOL STEM EXTRACT FOR ITS EFFECTS ON REPRODUCTIVE FUNCTIONS OF MALE RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 1, Jan. 2012, pp. 84-87, https://doi.org/10.25004/IJPSDR.2012.040116.
