THERAPEUTIC POTENTIAL OF OCIMUM SANCTUM IN PREVENTION AND TREATMENT OF CANCER AND EXPOSURE TO RADIATION: AN OVERVIEW
Abstract
Extensive studies, experimental and clinical prove that Tulsi possess anti-stress/adatogenic, antioxidant, immunomodulator and anti-radiation properties which also may help it to play major role in prevention and treatment of cancer. As Tulsi has health benefitting effects by reducing stress and improving both cellular and humoral immunity, its role in prevention and treatment of cancer cases may be a new approach in therapy of cancer and in prevention of ill effects of radiation. Studies in biological models like fibrosarcoma cell culture, papilomas in the skin of albino mice, mice having sarcoma - 180 solid tumors etc. provide proof for its anticancer activity. As it has been shown that Tulsi exhibit anticancer activity in animal models, studies were carried out in human cancer in vivo like human cell fibrosarcoma and in vitro in human cervical cancer cell line (HeLa) and human laryngeal epithelial carcinoma cell line (HEp-2) and it was found effective. Thus, this review is a concise version of Tulsi’s anti-cancer effect. The aim is to stimulate research in this field, prevent and treat human carcinoma by initiating detailed studies in this field. It will be a novel agent, safe and effective for humans suffering with cancer in general and as a specific agent. The research work which is going on this direction is a proof of benefits by reducing toxicity of chemo and radio therapies and providing better and healthier life style by Tulsi. Therefore, it was worthwhile to review its anticancer properties to give an overview of its status to scientist both modern and ancient (Ayurvedic).
Keywords:
Ocimum sanctum, chemical constituents, anticancer, adaptogen/ antistress, antioxidant, anti-radiation, immunomodulatorDOI
https://doi.org/10.25004/IJPSDR.2012.040202References
1. Moritz A. Cancer Is Not A Disease, 2nd Edition, Ener-Chi Wellness Press, USA, 2008, pp. 21-23.
2. Karthikeyan K, Gunasekaran P, Ramamurthy N, Govindasamy S. Anticancer Activity of Ocimum sanctum. Pharmaceutical Biology 1999; 37 (4):285-290.
3. Singh N. In the Symposium “Continuing Education Programme on Herbal Drug Research” held at Institute of Nuclear Medicine and Allied Sciences, DRDO, Delhi, India on 3-7 October 2005.
4. Singh N, Hoette Y, Miller R. Tulsi ‘The Mother Medicine of Nature’ 2nd Edition. International Institute of Herbal Medicine, Lucknow, 2010, pp. 28-47.
5. Phillip MP, Damodaran NP. Chemo-types of Ocimum sanctum Linn. Indian Perfumer 1985; 29:49-56.
6. Kothari SK, Bhattacharya AK, Ramesh S. Essential oil yield and quality of methyl eugenol rich O. tenuiflorum L.F. (syn Ocimum sanctum L.) grown in south India as influenced by method of harvest. J Chromatogr A. 2004; 1054:67-72.
7. Lawrence BM, Hogg JW, Terhune SJ, Pichitakul N. Essencial oils and their constituents. The oils of O. sanctum and O. basilicum from Thailand. Flav Industry 1972; 9:47-49.
8. Sukari MA, Rahmani M, Lee GB. Constituents of stem barks of Ocimum sanctum. Fitoterpia 1995; 66: 552-553.
9. Brophy JJ, Goldsack RJ, Clarkson JR. The essential oil of Ocimum tenuiflorum L. (Lamiaceae) growing in Nothern Australia. J Essent Oil Res. 1993; 5:459-461.
10. Nguyen H, Lemberkovics E, Tarr K, Mathe IJ, Petri G. A comperative study on formation of flavonoid, tannin, polyphenol contents in ontogenesis of O. basilicum L. Acta Agronomica Hungarica 1993; 42:41-50.
11. Skaltsa H, Philians S, Singh M. Phytochemical study of the leaves of Ocimum sanctum. Fitoterapia 1987; 8: 286.
12. Norr H, Wanger H. New constituents from O. sanctum. Planta Med 1992; 58:574.
13. Ravid Z, Putievsky E, Katzir I, Lewinsohn E. Enantiomeric composition of linalool in the essential oils of Ocimum species and in commercial Basil oils. Flav Frag. 1997; 12: 393-369.
14. Machado MIL, Silva MGV, Matos FJA, Craverio AA, Alencar WJ. Volatile constitution from leaves and inflorescence oil of Ocimum tenuiflorum L. F. (syn Ocimum sanctum L) grown in Northeastern Brazil. J essent oil Res. 1999; 11: 324-326.
15. Kicel A, Kurowska A, Kalemba D. Composition of the essential oil of Ocimum sanctum L. grown in Poland during vegetarian. J Essent Oil Res. 2005; 17:217-219.
16. Mondello L, Zappia G, Cotroneo A, Bonaccorsi L, Chowdhury JU, Yusuf M, Dugo G. Studies on the essential oil bearing plants of Bangladesh. Part 8. Composition of some Ocimum oils, O. basilicum L. var. purpurascens; O. sanctum L. green; O. sanctum L. Purple; O. americanum L., citral type; O. americanum L., camphor type. Flav Frag J. 2002; 17: 335-340.
17. Vina A, Murillo E. Essential oil composition from twelve varieties of basil (Ocimum spp.) grown in Colombia. J Braz Chem Soc. 2003; 14: 744-749.
18. Mondal S, Mirdha BR, Naik SN, Mahapatra SC. Antimicrobial activities of essential oils obtained from fresh and dried leaves of O. sanctum (L) against enteric bacteria and yeast. Editors, AK Yadav. In Proceedings of International Symposium on Medicinal and Nutraceutical Plants. Acta Hort .2007; 756; 267-269.
19. Dey BR, Choudhry M. Effect of plant development stage and some micronutrients on eugenol content in O. sanctum L. determination of eugenol by Folin-Ciocalteu reagent. Indian perfumer 1980; 24:199-203.
20. Dey BB, Choudhury MA. Essential oil of Ocimum sanctum L. and its antimicrobial activity. Indian Perfumer 1984; 28:82-87.
21. Maheshwari ML, Singh BM, Gupta R, Chien M. Essential oil of sacred Basil (Ocimum sanctum). Indian Perfumer. 1987; 31: 137-145.
22. Pareek SK, Maheswari ML, Gupta R. Domestication studies of O. sanctum for high oil and eugenol content. Indian pefumer. 1980; 24:93-100.
23. Pareek SK, maheswari ML, Gupta R. Oil content and its composition at different stages of growth in O. sanctum Lin. Indian Perfumer 1982; 26: 86-89.
24. Asthana OP, Gupta R. Effects of NP levels on Physiological parameters at growth stages in sacred basil (Ocimum sanctum Lin). Indian perfumer 1984; 28: 49-53.
25. Verma PK, Punia MS, Sharma GD, Talwar G. Evaluation of different species of Ocimum for their herb and oil yield under Haryana conditions. Indian Perfumer. 1989; 33: 79-83.
26. Gupta SC. Validation of herbage yield, oil yield and major component of various Ocimum species/ varieties (chemotypes) harvested at different stages of maturity. J Essent oil Res. 1996; 8:275-279.
27. Gupta SC. Population study and improvement of yield charecters in Ocimum sanctum Linn. Indian Perfumer. 1999; 36: 289-292.
28. Bhattacharya SK, Kaul PN, Rao BRR. Essential oils of Ocimum gratissimum L. and Ocimum tenuiflorum (Ocimum sanctum L) grown in Andhra Pradesh. Indian Perfumer 1996; 40: 73-75.
29. Raju PM, Ali M, Velasco- Negueruela A, Perez-Alonso MJ. Volatile constituents of the leaves of Ocimum sanctum L. J essent Oil Res. 1999; 11: 159-161.
30. Nair AGR, Gunasegaran R. Chemical investigation of certain south Indian Plants. Indian J Chem. 1982; 21 B: 979-980.
31. Skalta H, Tzakou O, Singh M. Polyphenols of O. sanctum from suriname. Pharmaceut Biol. 1999; 37:92-94.
32. Nadkarni GB, Patwardhan VA. Fatty oil from the seeds of O. sanctum Linn. (Tulsi). Cur Sci. 1952; 91: 68-69.
33. Singh S, Majumdar DK, Yadav MR. Chemical and pharmacological studies of O. sanctum fixed oil. Indian J Exp Biol. 1996; 34: 1212-1215.
34. Naredhirakannan RT, Subramanian S, Kandaswamy M. Mineral content of some medicinal plants used in the treatment of diabetes mellitus. Biol Trac Elem Res. 2005; 105:109-116.
35. Anonymous. Wealth of India, Publication and Information Directorate, CSIR, New Delhi, 1991, 7, pp. 79-89.
36. Rahman S, Islam R, Kamruzzaman M, Alam K, Jamal AHM. Ocimum sanctum L. A Review of Phytochemical and Pharmacological Profile. American Journal of Drug Discovery and Development 2011; 1-15.
37. Mondal S, Mirdha BR, Mahapatra SC. The Science behind sacredness of Tulsi (Ocimum sanctum Linn.). Indian J Physiol Pharmacol. 2009; 53(4): 291-306.
38. Godhwani S, Godhwani JL, Vyas DS. Ocimum sanctum-A preliminary study evaluating its immunoregulatory profile in albino rats. Journal of Ethnopharmacology 1988; 24: 193-198.
39. Robbins J. Losing a war we could prevent. In: Diet for a New America. Stillpoint Publishing, Meetinghouse Road Walpole, NH, 248, 1987.
40. Kim OK, Murakami A, Nakamura Y, Ohigashi H. Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 246.7 cells. Cancer let. 1998; 125(1-2):199-207.
41. Aruna K, Sivaramkrishnan VM. Plant products as protective agents against cancer. Ind. J. Exp. Biol. 1990; 28(11):1008-1011.
42. Prashar R, Kumar A, Banerjee S, Rao AR. Chemopreventive action by an extract from Ocimum sanctum on mouse skin papillomagenesis and its enhancement of skin glutathione S-transferase activity and acid soluble sulfydril level. Anticancer Drugs 1994; 5(5):567-572.
43. Sukumaran K, Unnikrishnan MC, Kuttan R. Inhibition of tumour promotion in mice by eugenol. Indian J Physiol Pharmacol. 1994; 38:306.
44. Kim SC, Magesh V, Jeong SJ, Lee HJ, Ahn KS, Lee HJ, Lee EO, Kim SH, Lee MH, Kim JH, Kim SH. Ethanol extract of Ocimum sanctum extracts anti metastatic activity through inactivation of matrix metalloproteinase-9 and enhancement of anti antioxidant enzyme. Food chem. Toxicol. 2010; 48(6):1478-1482.
45. Kathirvel P, Ravi S. Chemical composition of the essential oil from basil (Ocimum basilicum Linn.) and its in vitro cytotoxicity against HeLa and HEp-2 human cancer cell lines and NIH 3T3 mouse embryonic fibroblasts. Nat Prod Res. 2011; Sep 22. (Epub ahead of print).
46. Prakash J, Gupta SK. Chemopreventive activity of Ocimum sanctum seed oil. J Ethnopharmacol. 2000; 72 (1-2): 29-34.
47. Prakash P, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J Physiol Pharmacol. 2005; 49(2):125-31.
48. Ranga RS, Sowmyalakshmi S, Burikhanov R, Akbarsha MA, Chendil D. A herbal medicine for the treatment of lung cancer. Mol. Cell Biochem. 2005; 280: 125-133.
49. Prashar R, Kumar A, Hewer A, Cole KJ, Davis W, Phillips DH. Inhibition by an extract of Ocimum sanctum of DNA-binding activity of 7, 12-dimethylbenz [a] anthracene in rat hepatocytes in vitro. Cancer Lett. 1998; 128: 155-160.
50. Manikandan P, Letchoumy PV, Prathiba D, Nagini S. Proliferation, angiogenesis and apoptosis-associated proteins are molecular targets for chemoprevention of MNNG-induced gastric carcinogenesis by ethanolic Ocimum sanctum leaf extract. Singapore Med J. 2007; 48: 645-651.
51. Nakamura CV, Ishida K, Faccin LC, Filho BPD, Cortez DAG. In vitro activity of essential oil from Ocimum gratissimum L. against four Candida species. Res. Microbiol. 2004; 155: 579-586.
52. Singh V, Amdekar S, Verma O. Ocimum tanuiflorum (Tulsi): Bio-pharmacological activities. Pharmacology 2010; 1(10).
53. Govind P, Madhuri S. Medicinal plants: Better remedy for neoplasm. Indian Drug 2006; 43(11): 869-874.
54. Kathiresan K, Guanasekan P, Rammurthy N, Govidswami S. Anticancer activity of Ocimum sanctum. Pharmaceutical Biology1999; 37(4):285-290.
55. Madhuri S. Studies on oestrogen induced uterine and ovarian carcinogenesis and effect of ProImmu in rats. Ph.D. thesis, Rani Durgavati Vishwa Vidyalaya, Jabalpur, MP, India: 2008.
56. Madhuri S, Govind P. Effect of ProImmu, a herbal drug on estrogen caused uterine and ovarian cytotoxicity. Biomed. 2010; 5(1):57-62.
57. Govind P. An overview on certain anticancer natural products. J Pharm Res. 2009; 2(12): 1799-1803.
58. Govind P, Madhuri S. Autochthonous herbal products in the treatment of cancer. Phytomedica 2006; 7:99-104.
59. Aruna K, Sivaramakrishnan VM. Anticarcinogenic effects of some Indian plants products. Food ChemToxicol. 1992; 30:953.
60. Devi PU. Radioprotective, anticarcinogenic and antioxidant properties of the Indian holy basil, Ocimum sanctum (Tulasi). Indian J Exp Biol. 2001; 39:185-190.
61. Karthikeyan K, Ravichadran P, Govindasamy S. Chemopreventive effect of Ocimum sanctum on DMBA-induced hamster buccal pouch carcinogenesis. Oral Oncol. 1999; 35(1): 112-119.
62. Somkuwar AP. Studies on anticancer effects of Ocimum sanctum and Withania somnifera on experimentally induced cancer in mice. Ph.D. thesis, JNKVV, Jabalpur, MP, India: 2003.
63. Manikandan P, Murugan RS, Abbas H, Abraham SK, Nagini S. Ocimum sanctum Linn. (Holy Basil) ethanolic leaf extract protects against 7, 12-dimethylbenz (a) anthracene-induced genotoxicity, oxidative stress, and imbalance in xenobiotic-metabolizing enzymes. J Med Food 2007; 10:495-502.
64. Manikandan P, Vidjaya Letchoumy P, Prathiba D, Nagini S. Combinatorial chemopreventive effect of Azadirachta indica and Ocimum sanctum on oxidant-antioxidant status, cell proliferation, apoptosis and angiogenesis in a rat forestomach carcinogenesis model. Singapore Med J. 2008; 49 (10): 814.
65. Prashar R, Kumar A. Chemopreventive action of Ocimum sanctum on 2, 12- dimethylbenz (a) anthracene (DMBA) induced papillomagenesis in the skin of mice. Int J Pharmacog. 1995; 33: 181.
66. Prakash J, Gupta SK, Singh N, Kochupillai V, Gupta YK. Antiproliferative and chemopreventive activity of Ocimum sanctum Linn. Int J Med Biol Environ. 1999; 27:165.
67. Singh N, Gupta ML, Das M, Kohli RP. Preventive effect of some indigenous drugs on stress and aspirin induced gastric ulcer in albino rats. In the Proceeding Decennial Conference. Ind. Pharmacol. Sos, Calcutta 1977, Abstract No. 171.
68. Singh N, Nath R, Kohli RP. Experimental evaluation of adaptogenic properties of Ocimum sanctum. In the Proceeding Decennial Conference. Ind. Pharmacol. Sos, Calcutta 1977, Abstract No. 127.
69. Singh SP, Singh N. Experimental Evaluation of Adaptogenic Properties of Ocimum sanctum. Ind J Pharmacol. 1978; 10:74.
70. Bhargava KP, Singh N. Anti-stress activity of Ocimum sanctum Linn. Indian J Med Res. 1981:73; 443-451.
71. Srivastava AK, Singh N, Bhargava KP. “Pharmacology of Stress” Doctor of Medicine (Thesis MD), Lucknow University, India, 1984.
72. Singh N. A new concept on the possible therapy of stress disease with “adaptogen” (Anti-stress Drugs) of indigenous plant origin. Curr. Med. Prac. 1981; 25: 50-55.
73. Singh SP, Sinha KN, Singh N, Kohli RP. Innula racemosa (Pushkarmool), Terminalia belerica (Vibhitiki) and Ocimum sanctum (Tulsi) - A preliminary clinical trial in asthma patients. Proc. Int Sem Clin Pharmacol. Dev Count. KGMC, Lucknow, India (editors-Saxena RC, Gupta TK and Dixit KS). 1986; 1:18-21.
74. Singh N. A comperative evaluation of the effect of some species of Ocimum sanctum on anoxia tolerance in albino rats. 36th Ann. Cong. Med. Plant Res., Gesellschaft Fuer Arzeneipflanzenforschung, Freiburg, 1988, pp.28.
75. Singh N, Mishra N. Experimental Methods- Tools for assessment of anti-stress activity in medicinal plants. J Bio Chem Res. 1993; 12(182):124-127.
76. Dixit KS, Singh SP, Sinha KN, Singh N, Kohli RP. Inula racemosa (pushkarmul), Terminalia belerica (Vibhitki) and Ocimum sanctum (Tulsi) - A preliminary clinical trial in asthma patients. In Proc. Int. Sem. Clin. Pharmacol. Dev. Count. KGMC, Lucknow, India. 1986; 2:22-27.
77. Mishra N, Srivastava AK, Dixit KS, Singh N, Gupta GP. Plant drugs and biochemical changes during stress reaction. Physiology of Human Performance. In Proc. Nat. Symp. Physiol. Hum. Perfor. (editors-Sawhney RC, Sridharan K and Selvamurthy W) Publisher: Defence Institute of Physiology and Allied Science, Defence Research and Development Organization (DRDO), Govt. of India, Delhi, 1987, pp. 104-108.
78. Singh N, Misra N, Srivastava AK, Dixit KS, Gupta GP. Effects of anti-stress plants on biochemical changes during stress reaction. Ind. J. Pharmacol. 1991; 23(3):137-142.
79. Kalsi R, Singh N, Gupta GP. Effects of stress and anti-stress drugs on succinate dehydrogenase enzyme (SDH) in rat brain (A possible role of SDH in stress adaptation phenomenon). Physiology of Human Performance. In Proc. Nat. Symp. Physiol. Hum. Perfor. (editors-Sawhney RC, Sridharan K and Selvamurthy W) Publisher: Defence Institute of Physiology and Allied Science, Defence Research and Development Organization (DRDO), Govt. of India, Delhi, 1987, pp. 114-117.
80. Robbins SR, Angell M, Kumar V. Disease at the Cellular Level: Free radical Mediation of Cell injury. In: basic Pathology. 3rd edn. WS Saunders Company Philadelphia Press. Printed in Japan. Igaku- Shoin/ Saunders international Edition. 1982, pp. 9-13.
81. Jyoti S, Satendra S, Sushma S, Anjana T, Shashi S. Antistressor activity of Ocimum sanctum (Tulsi) against experimentally induced oxidative stress in rabbits. Methods Find Exp Clin Pharmacol. 2007; 29(6):411-6.
82. Panda S, Kar A. O. sanctum leaf extract in the regulation of thyroid function in the male mouse. Pharmacol Res. 1998, 38(2):107-110.
83. Devi PU, Gonasoundari A. Radioprotective effect of leaf extract of Indian Medicinal Plant Ocimum sanctum. Indian J Exp Biol. 1995; 33:205.
84. Devi PU, Gonasoundari A, Vrinda B, Srinivasan KK, Unnikrishanan MK. Radiation protection by the Ocimum sanctum flavonoids orientin and vicenin: Mechanism of action. Radiat Res. 2000; 154(4): 455-460.
85. Gonasoundari A, Devi PU, Rao BSS. Enhancement of bone marrow radioprotection and reduction of WR-2721 toxicity by Ocimum sanctum. Mutat Res. 1998; 397:303.
86. Bhartiya US, Raut YS, Joseph LJ. Protective effect of Ocimum sanctum L after high-dose 131iodine exposure in mice: An in-vivo study. Indian J Exp Biol. 2006; 44:647-52.
87. Subramanian M, Chintalwar GJ, Chattopadhyay S. Antioxidant and radioprotective properties of an Ocimum sanctum polysaccharide. Redox Rep. 2005; 10:257-264.
88. Vrinda B, Uma Devi P. Radiation protection of human lymphocyte chromosomes in-vitro by orientin and vicenin. Mutat Res. 2001; 498:39-46.
89. Devi PU, Ganasoundari A. Modulation of glutathione and antioxidant enzymes by Ocimum sanctum and its role in protection against radiation injury. Indian J Exp Biol. 1999; 37:262-268.
90. Devi PU, Bisht KS, Vinitha M. A comparative study of radioprotection by Ocimum flavonoids and synthetic aminothiol protectors in the mouse. Br J Radiol. 1998; 71:782-4.
91. Ganasoundari A, Devi PU, Rao BS. Enhancement of bone marrow radioprotection and reduction of WR-2721 toxicity by Ocimum sanctum. Mutat Res. 1998; 397:303-312.
92. Saija A, Scalese M, Lanza M, Marzillo D, Bonina F, Castelli F. Flavonoids as antioxidant agents: Importance of their interaction with biomembrane. Free Rad Biol Med.1995; 19: 481.
93. Kelm MA, Nair MG, Strasburg GM, DeWitt DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn. Phytomedicine 2000; 7(1):7-13.
94. Trevisan MT, Vasconcelos Silva MG, Pfundstein B, Spiegelhalder B, Owen RW. Characterization of the volatile pattern and antioxidant capacity of essential oils from different species of the genus Ocimum. J Agric Food Chem. 2006; 54:4378-4382.
95. Gupta S, Mediratta PK, Singh S, Sharma KK, Shukla R. Antidiabetic, antihypercholesterolaemic and antioxidant effect of Ocimum sanctum (Linn) seed oil. Indian J Exp Biol. 2006; 44:300-4.
96. Yanpallewar SU, Rai S, Kumar M, Acharya SB. Evaluation of antioxidant and neuroprotective effect of Ocimum sanctum on transient cerebral ischemia and long-term cerebral hypoperfusion. Pharmacol Biochem Behav. 2004; 79:155-64.
97. Mediratta PK, Dewan V, Bhattacharya SK, Gupta VS, Maiti PC, Sen P. Effect of Ocimum sanctum Linn on humoral immune responses. Indian J Med Res. 1988; 87:384-6.
98. Satayavanti GV, Raina MK, Sharma M. Medical plants of India published by ICMR, New Delhi, 1976.
99. Medrita PK, Dewan V, Bhattacharya SK, Gupta VS, Maiti PC, Sen P. Effect of Ocimum sanctum Linn. on humoral immune response. Ind J. Med. Res. 1987, 87:384.
100. Medrita PK, Sharma KK. Effect of essential oil of the leaves and fixed oil of the seeds of Ocimum sanctum on immune responses. J Med Aro Plant Sci. 2000; 22:694-700.
101. Mukherjee R, Dash PK, Ram GC. Immunotherapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis. Res Vet Sci. 2005; 79:37-43.
102. Mediratta PK, Sharma KK, Singh S. Evaluation of immunomodulatory potential of Ocimum sanctum seed oil and its possible mechanism of action. J Ethnopharmacol. 2002; 80:15-20.
103. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics. CA cancer J Clin. 2007; 57:43-66.
104. Aggarwal BB, Ichikawa H, Garodia P, Weerasinghe P, Sethi G, Bhatt ID, Pandey MK, Shishodia S, Nair MG. From traditional Ayurvedic medicine to modern medicine: identification of therapeutic targets for suppression of inflammation and cancer. Expert Opin Ther Targets. 2006; 10 (1): 87-118.
105. Kirtikar KR, Basu BD. Ocimum sanctum in Indian medicinal plants. 1933, 3, pp. 1965.
106. Carak Samhita (1000 B.C.) Chikitsa Sthana Section of Therapeutics, published by Ayurvedic Society of India, 1949; 3: 1278.
107. Nadkarni KM. Indian Materia Medica. Edn 3, Vol. I, Popular Prakashan, Mumbai, 1954, pp. 242-246.
108. Malviya BK, Gupta PL. Growth promoting properties of Ocimum sanctum. Indian J Pharmacy 1971; 33:126.
109. Singh TJ, Gupta PD, Khan SY, Misra KC. Preliminary pharmacological investigation of O. sanctum. India J Pharmacy 1970; 32: 93.
110. Krishnamurthy TR. Some pharmacological action of an extract of Ocimum sanctum. Indian J Physiol Pharmacol. 1959; 3: 92.
111. Vohra SB, Garg SK, Chowdhury RP. Effect of 6 indigenous plants on early pregnancy in albinorats. Indian J Pharmacy. 1968; 30:287.
112. Balta SK, Santhakumari G. The antifertility effect of O. sanctum and Hibiscus rasasinensis. Indian J med Res. 1971; 59: 777.
113. Palit G, Singh SP, Singh N, Kohli RP, Bhargava KP. Antiasthamatic potentials of O. sanctum and T. belerica. In Proc. 14th Convention Indian Coll. Aller. Appl. Immunol., 1980, pp-10.
114. Palit G, Singh SP, Singh N, Kohli RP, Bhargava KP. An experimental evaluation of Antiasthamatic plant drugs from the ancient ayurvedic medicine. Asp. Alter. Appl. Immunol. 1983; 16:36-41.
115. Siurin SA. Effects of essential oil on lipid peroxidation and lipid metabolism in patients with chronic bronchitis, Klin Med (Mosk). 1997; 75(10):43-5.
116. Singh N. A pharmaco-clinical evaluation of some Ayurvedic crude plant drugs as anti-stress agents and their usefulness in some stress diseases of man. Ann Nat Acad Ind Med. 1986; 2(1):14-26.
117. Das SK, Chandra A, Agarwal SS, Singh N. Ocimum sanctum (Tulsi) in the treatment of viral encephalitis (a prtelinary clinical trial). The Antiseptic 1983; 1-5.
118. Jalil A. Clinical trial of O. sanctum (Tulsi) in peptic ulcerand hyperacidity patients. J Res Ind Med. 1970; 4 (2):238-239.
119. Mediratta PK, Dewan V, Bhattacharya SK, Gupta VS, Malti PC, Sen P. Effect of O. sanctum Linn. on humoral immune response. Ind J Med Res. 1987; 87:384.
120. Singh N. A pharmaco- clinical evaluation of some ayurvedic crude plant drugs as anti stress agents and their usefulness in some stress disease of man.Ann. Nat Acad Ind Med. 1986; 2(1):14-26.
121. Singh N, Misra N. Stress disease and their possible remedy by anti-stress drugs (Adaptogens/ Staminators) of plant origin. Physiology of Human Performance. In Proc Nat Symp Physiol Hum Perfor. (Sawhney RC, Sridharan K and Selvamurthy W, eds) Publisher: Defence Institute of Physiology and Allied Science, Defence Research and Development Organization (DRDO), Govt. of India, Delhi, 1987, pp. 89-94.
122. Agarwal P, Rai V, Singh RB. Randomized placebo- controlled single blind trial of holy basil leaves in patients with non insulin-dependent diabetes mellitus. Int J Clin Pharmacol Ther. 1996; 34(9):406-409.
123. Puspangdan P, Sobti SN. Medicinal Properties of Ocimum (Tulsi) species and some recent investigation of their efficacy. Ind. Drugs 1977; 14 (11):207.
124. Patel VK, Bhatt HVK. Folklore therapeutics indigenous plants in periodontal disorder in India (review experimental and clinical approach). Int J Clin Pharmacol Ther Toxicol. 1988; 26 (4):176-184.
125. Adam US. Oceanite: A Maritime Union of India Publication, 1997; 197:37.
126. Singh N, Abbas SS. Effect of Ocimum sanctum (Tulsi) in sex-related chronic fatigue syndrome (SRCFS) in young Indians. J. Bio Chem Sci, 1995b, 14:184-187.
127. Sivarajan VV, Balachandran I. Tulsi. In: Ayurvedic Drugs and their Plant Sources. Oxford FBH Publishing Co. Pvt. Ltd., New Delhi, India, 1994, pp. 485- 486.
128. Gupta KC, Vishwanathan. A short note on Antitubercular substance from Ocimum sanctum. Antibiotic and Chemotherapy. 1955; 5:33.
129. Singh SP, Singh N. Experimental evaluation of adaptogenic properties of Ocimum sanctum. Ind. J. Pharmacol. 1978; 10:74.
130. Bhargava KP, Singh N. Anti-stress activity of Ocimum sanctum (Linn.). Ind. J. Med. Res.1981; 73:443-451.
131. Bhargava KP, Singh N. Comparative evaluation of anti-stress activity of Eleutherococcus senticosus, Panax ginseng and Ocimum sanctum. New Data on Eleutherococcus Moscow (U.S.S.R), 1984, pp. 181-89.
132. Bhalla M, Singh N. A clinical study of anticancer properties of Organic Ashwagandha, Tulsi and Wheat Grass. In the proceeding of the National Seminar- Brain Storming Session on Integrated Therapeutic Approach in the Management of Cancer, 26-27 march, 2011, Lucknow, pp.35.
133. Singh N, Hoette Y, Miller R. Tulsi ‘The Mother Medicine of Nature’ Ist Edn, International Institute of Herbal Medicine, Lucknow, 2002, pp. 29-42.
134. Singh N, Gilca M. Herbal Medicine – Science embraces tradition – a new insight into the ancient Ayurveda. Lambert Academic Publishing (Germany), 2010, pp. 315-322.
135. Jogetia GC, Devi PU, Singhatgeri MK, Singh N, Kohli, R.P. Proc. 56th Annual Session of National Academy of Sciences India, 1986, pp.40.
2. Karthikeyan K, Gunasekaran P, Ramamurthy N, Govindasamy S. Anticancer Activity of Ocimum sanctum. Pharmaceutical Biology 1999; 37 (4):285-290.
3. Singh N. In the Symposium “Continuing Education Programme on Herbal Drug Research” held at Institute of Nuclear Medicine and Allied Sciences, DRDO, Delhi, India on 3-7 October 2005.
4. Singh N, Hoette Y, Miller R. Tulsi ‘The Mother Medicine of Nature’ 2nd Edition. International Institute of Herbal Medicine, Lucknow, 2010, pp. 28-47.
5. Phillip MP, Damodaran NP. Chemo-types of Ocimum sanctum Linn. Indian Perfumer 1985; 29:49-56.
6. Kothari SK, Bhattacharya AK, Ramesh S. Essential oil yield and quality of methyl eugenol rich O. tenuiflorum L.F. (syn Ocimum sanctum L.) grown in south India as influenced by method of harvest. J Chromatogr A. 2004; 1054:67-72.
7. Lawrence BM, Hogg JW, Terhune SJ, Pichitakul N. Essencial oils and their constituents. The oils of O. sanctum and O. basilicum from Thailand. Flav Industry 1972; 9:47-49.
8. Sukari MA, Rahmani M, Lee GB. Constituents of stem barks of Ocimum sanctum. Fitoterpia 1995; 66: 552-553.
9. Brophy JJ, Goldsack RJ, Clarkson JR. The essential oil of Ocimum tenuiflorum L. (Lamiaceae) growing in Nothern Australia. J Essent Oil Res. 1993; 5:459-461.
10. Nguyen H, Lemberkovics E, Tarr K, Mathe IJ, Petri G. A comperative study on formation of flavonoid, tannin, polyphenol contents in ontogenesis of O. basilicum L. Acta Agronomica Hungarica 1993; 42:41-50.
11. Skaltsa H, Philians S, Singh M. Phytochemical study of the leaves of Ocimum sanctum. Fitoterapia 1987; 8: 286.
12. Norr H, Wanger H. New constituents from O. sanctum. Planta Med 1992; 58:574.
13. Ravid Z, Putievsky E, Katzir I, Lewinsohn E. Enantiomeric composition of linalool in the essential oils of Ocimum species and in commercial Basil oils. Flav Frag. 1997; 12: 393-369.
14. Machado MIL, Silva MGV, Matos FJA, Craverio AA, Alencar WJ. Volatile constitution from leaves and inflorescence oil of Ocimum tenuiflorum L. F. (syn Ocimum sanctum L) grown in Northeastern Brazil. J essent oil Res. 1999; 11: 324-326.
15. Kicel A, Kurowska A, Kalemba D. Composition of the essential oil of Ocimum sanctum L. grown in Poland during vegetarian. J Essent Oil Res. 2005; 17:217-219.
16. Mondello L, Zappia G, Cotroneo A, Bonaccorsi L, Chowdhury JU, Yusuf M, Dugo G. Studies on the essential oil bearing plants of Bangladesh. Part 8. Composition of some Ocimum oils, O. basilicum L. var. purpurascens; O. sanctum L. green; O. sanctum L. Purple; O. americanum L., citral type; O. americanum L., camphor type. Flav Frag J. 2002; 17: 335-340.
17. Vina A, Murillo E. Essential oil composition from twelve varieties of basil (Ocimum spp.) grown in Colombia. J Braz Chem Soc. 2003; 14: 744-749.
18. Mondal S, Mirdha BR, Naik SN, Mahapatra SC. Antimicrobial activities of essential oils obtained from fresh and dried leaves of O. sanctum (L) against enteric bacteria and yeast. Editors, AK Yadav. In Proceedings of International Symposium on Medicinal and Nutraceutical Plants. Acta Hort .2007; 756; 267-269.
19. Dey BR, Choudhry M. Effect of plant development stage and some micronutrients on eugenol content in O. sanctum L. determination of eugenol by Folin-Ciocalteu reagent. Indian perfumer 1980; 24:199-203.
20. Dey BB, Choudhury MA. Essential oil of Ocimum sanctum L. and its antimicrobial activity. Indian Perfumer 1984; 28:82-87.
21. Maheshwari ML, Singh BM, Gupta R, Chien M. Essential oil of sacred Basil (Ocimum sanctum). Indian Perfumer. 1987; 31: 137-145.
22. Pareek SK, Maheswari ML, Gupta R. Domestication studies of O. sanctum for high oil and eugenol content. Indian pefumer. 1980; 24:93-100.
23. Pareek SK, maheswari ML, Gupta R. Oil content and its composition at different stages of growth in O. sanctum Lin. Indian Perfumer 1982; 26: 86-89.
24. Asthana OP, Gupta R. Effects of NP levels on Physiological parameters at growth stages in sacred basil (Ocimum sanctum Lin). Indian perfumer 1984; 28: 49-53.
25. Verma PK, Punia MS, Sharma GD, Talwar G. Evaluation of different species of Ocimum for their herb and oil yield under Haryana conditions. Indian Perfumer. 1989; 33: 79-83.
26. Gupta SC. Validation of herbage yield, oil yield and major component of various Ocimum species/ varieties (chemotypes) harvested at different stages of maturity. J Essent oil Res. 1996; 8:275-279.
27. Gupta SC. Population study and improvement of yield charecters in Ocimum sanctum Linn. Indian Perfumer. 1999; 36: 289-292.
28. Bhattacharya SK, Kaul PN, Rao BRR. Essential oils of Ocimum gratissimum L. and Ocimum tenuiflorum (Ocimum sanctum L) grown in Andhra Pradesh. Indian Perfumer 1996; 40: 73-75.
29. Raju PM, Ali M, Velasco- Negueruela A, Perez-Alonso MJ. Volatile constituents of the leaves of Ocimum sanctum L. J essent Oil Res. 1999; 11: 159-161.
30. Nair AGR, Gunasegaran R. Chemical investigation of certain south Indian Plants. Indian J Chem. 1982; 21 B: 979-980.
31. Skalta H, Tzakou O, Singh M. Polyphenols of O. sanctum from suriname. Pharmaceut Biol. 1999; 37:92-94.
32. Nadkarni GB, Patwardhan VA. Fatty oil from the seeds of O. sanctum Linn. (Tulsi). Cur Sci. 1952; 91: 68-69.
33. Singh S, Majumdar DK, Yadav MR. Chemical and pharmacological studies of O. sanctum fixed oil. Indian J Exp Biol. 1996; 34: 1212-1215.
34. Naredhirakannan RT, Subramanian S, Kandaswamy M. Mineral content of some medicinal plants used in the treatment of diabetes mellitus. Biol Trac Elem Res. 2005; 105:109-116.
35. Anonymous. Wealth of India, Publication and Information Directorate, CSIR, New Delhi, 1991, 7, pp. 79-89.
36. Rahman S, Islam R, Kamruzzaman M, Alam K, Jamal AHM. Ocimum sanctum L. A Review of Phytochemical and Pharmacological Profile. American Journal of Drug Discovery and Development 2011; 1-15.
37. Mondal S, Mirdha BR, Mahapatra SC. The Science behind sacredness of Tulsi (Ocimum sanctum Linn.). Indian J Physiol Pharmacol. 2009; 53(4): 291-306.
38. Godhwani S, Godhwani JL, Vyas DS. Ocimum sanctum-A preliminary study evaluating its immunoregulatory profile in albino rats. Journal of Ethnopharmacology 1988; 24: 193-198.
39. Robbins J. Losing a war we could prevent. In: Diet for a New America. Stillpoint Publishing, Meetinghouse Road Walpole, NH, 248, 1987.
40. Kim OK, Murakami A, Nakamura Y, Ohigashi H. Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 246.7 cells. Cancer let. 1998; 125(1-2):199-207.
41. Aruna K, Sivaramkrishnan VM. Plant products as protective agents against cancer. Ind. J. Exp. Biol. 1990; 28(11):1008-1011.
42. Prashar R, Kumar A, Banerjee S, Rao AR. Chemopreventive action by an extract from Ocimum sanctum on mouse skin papillomagenesis and its enhancement of skin glutathione S-transferase activity and acid soluble sulfydril level. Anticancer Drugs 1994; 5(5):567-572.
43. Sukumaran K, Unnikrishnan MC, Kuttan R. Inhibition of tumour promotion in mice by eugenol. Indian J Physiol Pharmacol. 1994; 38:306.
44. Kim SC, Magesh V, Jeong SJ, Lee HJ, Ahn KS, Lee HJ, Lee EO, Kim SH, Lee MH, Kim JH, Kim SH. Ethanol extract of Ocimum sanctum extracts anti metastatic activity through inactivation of matrix metalloproteinase-9 and enhancement of anti antioxidant enzyme. Food chem. Toxicol. 2010; 48(6):1478-1482.
45. Kathirvel P, Ravi S. Chemical composition of the essential oil from basil (Ocimum basilicum Linn.) and its in vitro cytotoxicity against HeLa and HEp-2 human cancer cell lines and NIH 3T3 mouse embryonic fibroblasts. Nat Prod Res. 2011; Sep 22. (Epub ahead of print).
46. Prakash J, Gupta SK. Chemopreventive activity of Ocimum sanctum seed oil. J Ethnopharmacol. 2000; 72 (1-2): 29-34.
47. Prakash P, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J Physiol Pharmacol. 2005; 49(2):125-31.
48. Ranga RS, Sowmyalakshmi S, Burikhanov R, Akbarsha MA, Chendil D. A herbal medicine for the treatment of lung cancer. Mol. Cell Biochem. 2005; 280: 125-133.
49. Prashar R, Kumar A, Hewer A, Cole KJ, Davis W, Phillips DH. Inhibition by an extract of Ocimum sanctum of DNA-binding activity of 7, 12-dimethylbenz [a] anthracene in rat hepatocytes in vitro. Cancer Lett. 1998; 128: 155-160.
50. Manikandan P, Letchoumy PV, Prathiba D, Nagini S. Proliferation, angiogenesis and apoptosis-associated proteins are molecular targets for chemoprevention of MNNG-induced gastric carcinogenesis by ethanolic Ocimum sanctum leaf extract. Singapore Med J. 2007; 48: 645-651.
51. Nakamura CV, Ishida K, Faccin LC, Filho BPD, Cortez DAG. In vitro activity of essential oil from Ocimum gratissimum L. against four Candida species. Res. Microbiol. 2004; 155: 579-586.
52. Singh V, Amdekar S, Verma O. Ocimum tanuiflorum (Tulsi): Bio-pharmacological activities. Pharmacology 2010; 1(10).
53. Govind P, Madhuri S. Medicinal plants: Better remedy for neoplasm. Indian Drug 2006; 43(11): 869-874.
54. Kathiresan K, Guanasekan P, Rammurthy N, Govidswami S. Anticancer activity of Ocimum sanctum. Pharmaceutical Biology1999; 37(4):285-290.
55. Madhuri S. Studies on oestrogen induced uterine and ovarian carcinogenesis and effect of ProImmu in rats. Ph.D. thesis, Rani Durgavati Vishwa Vidyalaya, Jabalpur, MP, India: 2008.
56. Madhuri S, Govind P. Effect of ProImmu, a herbal drug on estrogen caused uterine and ovarian cytotoxicity. Biomed. 2010; 5(1):57-62.
57. Govind P. An overview on certain anticancer natural products. J Pharm Res. 2009; 2(12): 1799-1803.
58. Govind P, Madhuri S. Autochthonous herbal products in the treatment of cancer. Phytomedica 2006; 7:99-104.
59. Aruna K, Sivaramakrishnan VM. Anticarcinogenic effects of some Indian plants products. Food ChemToxicol. 1992; 30:953.
60. Devi PU. Radioprotective, anticarcinogenic and antioxidant properties of the Indian holy basil, Ocimum sanctum (Tulasi). Indian J Exp Biol. 2001; 39:185-190.
61. Karthikeyan K, Ravichadran P, Govindasamy S. Chemopreventive effect of Ocimum sanctum on DMBA-induced hamster buccal pouch carcinogenesis. Oral Oncol. 1999; 35(1): 112-119.
62. Somkuwar AP. Studies on anticancer effects of Ocimum sanctum and Withania somnifera on experimentally induced cancer in mice. Ph.D. thesis, JNKVV, Jabalpur, MP, India: 2003.
63. Manikandan P, Murugan RS, Abbas H, Abraham SK, Nagini S. Ocimum sanctum Linn. (Holy Basil) ethanolic leaf extract protects against 7, 12-dimethylbenz (a) anthracene-induced genotoxicity, oxidative stress, and imbalance in xenobiotic-metabolizing enzymes. J Med Food 2007; 10:495-502.
64. Manikandan P, Vidjaya Letchoumy P, Prathiba D, Nagini S. Combinatorial chemopreventive effect of Azadirachta indica and Ocimum sanctum on oxidant-antioxidant status, cell proliferation, apoptosis and angiogenesis in a rat forestomach carcinogenesis model. Singapore Med J. 2008; 49 (10): 814.
65. Prashar R, Kumar A. Chemopreventive action of Ocimum sanctum on 2, 12- dimethylbenz (a) anthracene (DMBA) induced papillomagenesis in the skin of mice. Int J Pharmacog. 1995; 33: 181.
66. Prakash J, Gupta SK, Singh N, Kochupillai V, Gupta YK. Antiproliferative and chemopreventive activity of Ocimum sanctum Linn. Int J Med Biol Environ. 1999; 27:165.
67. Singh N, Gupta ML, Das M, Kohli RP. Preventive effect of some indigenous drugs on stress and aspirin induced gastric ulcer in albino rats. In the Proceeding Decennial Conference. Ind. Pharmacol. Sos, Calcutta 1977, Abstract No. 171.
68. Singh N, Nath R, Kohli RP. Experimental evaluation of adaptogenic properties of Ocimum sanctum. In the Proceeding Decennial Conference. Ind. Pharmacol. Sos, Calcutta 1977, Abstract No. 127.
69. Singh SP, Singh N. Experimental Evaluation of Adaptogenic Properties of Ocimum sanctum. Ind J Pharmacol. 1978; 10:74.
70. Bhargava KP, Singh N. Anti-stress activity of Ocimum sanctum Linn. Indian J Med Res. 1981:73; 443-451.
71. Srivastava AK, Singh N, Bhargava KP. “Pharmacology of Stress” Doctor of Medicine (Thesis MD), Lucknow University, India, 1984.
72. Singh N. A new concept on the possible therapy of stress disease with “adaptogen” (Anti-stress Drugs) of indigenous plant origin. Curr. Med. Prac. 1981; 25: 50-55.
73. Singh SP, Sinha KN, Singh N, Kohli RP. Innula racemosa (Pushkarmool), Terminalia belerica (Vibhitiki) and Ocimum sanctum (Tulsi) - A preliminary clinical trial in asthma patients. Proc. Int Sem Clin Pharmacol. Dev Count. KGMC, Lucknow, India (editors-Saxena RC, Gupta TK and Dixit KS). 1986; 1:18-21.
74. Singh N. A comperative evaluation of the effect of some species of Ocimum sanctum on anoxia tolerance in albino rats. 36th Ann. Cong. Med. Plant Res., Gesellschaft Fuer Arzeneipflanzenforschung, Freiburg, 1988, pp.28.
75. Singh N, Mishra N. Experimental Methods- Tools for assessment of anti-stress activity in medicinal plants. J Bio Chem Res. 1993; 12(182):124-127.
76. Dixit KS, Singh SP, Sinha KN, Singh N, Kohli RP. Inula racemosa (pushkarmul), Terminalia belerica (Vibhitki) and Ocimum sanctum (Tulsi) - A preliminary clinical trial in asthma patients. In Proc. Int. Sem. Clin. Pharmacol. Dev. Count. KGMC, Lucknow, India. 1986; 2:22-27.
77. Mishra N, Srivastava AK, Dixit KS, Singh N, Gupta GP. Plant drugs and biochemical changes during stress reaction. Physiology of Human Performance. In Proc. Nat. Symp. Physiol. Hum. Perfor. (editors-Sawhney RC, Sridharan K and Selvamurthy W) Publisher: Defence Institute of Physiology and Allied Science, Defence Research and Development Organization (DRDO), Govt. of India, Delhi, 1987, pp. 104-108.
78. Singh N, Misra N, Srivastava AK, Dixit KS, Gupta GP. Effects of anti-stress plants on biochemical changes during stress reaction. Ind. J. Pharmacol. 1991; 23(3):137-142.
79. Kalsi R, Singh N, Gupta GP. Effects of stress and anti-stress drugs on succinate dehydrogenase enzyme (SDH) in rat brain (A possible role of SDH in stress adaptation phenomenon). Physiology of Human Performance. In Proc. Nat. Symp. Physiol. Hum. Perfor. (editors-Sawhney RC, Sridharan K and Selvamurthy W) Publisher: Defence Institute of Physiology and Allied Science, Defence Research and Development Organization (DRDO), Govt. of India, Delhi, 1987, pp. 114-117.
80. Robbins SR, Angell M, Kumar V. Disease at the Cellular Level: Free radical Mediation of Cell injury. In: basic Pathology. 3rd edn. WS Saunders Company Philadelphia Press. Printed in Japan. Igaku- Shoin/ Saunders international Edition. 1982, pp. 9-13.
81. Jyoti S, Satendra S, Sushma S, Anjana T, Shashi S. Antistressor activity of Ocimum sanctum (Tulsi) against experimentally induced oxidative stress in rabbits. Methods Find Exp Clin Pharmacol. 2007; 29(6):411-6.
82. Panda S, Kar A. O. sanctum leaf extract in the regulation of thyroid function in the male mouse. Pharmacol Res. 1998, 38(2):107-110.
83. Devi PU, Gonasoundari A. Radioprotective effect of leaf extract of Indian Medicinal Plant Ocimum sanctum. Indian J Exp Biol. 1995; 33:205.
84. Devi PU, Gonasoundari A, Vrinda B, Srinivasan KK, Unnikrishanan MK. Radiation protection by the Ocimum sanctum flavonoids orientin and vicenin: Mechanism of action. Radiat Res. 2000; 154(4): 455-460.
85. Gonasoundari A, Devi PU, Rao BSS. Enhancement of bone marrow radioprotection and reduction of WR-2721 toxicity by Ocimum sanctum. Mutat Res. 1998; 397:303.
86. Bhartiya US, Raut YS, Joseph LJ. Protective effect of Ocimum sanctum L after high-dose 131iodine exposure in mice: An in-vivo study. Indian J Exp Biol. 2006; 44:647-52.
87. Subramanian M, Chintalwar GJ, Chattopadhyay S. Antioxidant and radioprotective properties of an Ocimum sanctum polysaccharide. Redox Rep. 2005; 10:257-264.
88. Vrinda B, Uma Devi P. Radiation protection of human lymphocyte chromosomes in-vitro by orientin and vicenin. Mutat Res. 2001; 498:39-46.
89. Devi PU, Ganasoundari A. Modulation of glutathione and antioxidant enzymes by Ocimum sanctum and its role in protection against radiation injury. Indian J Exp Biol. 1999; 37:262-268.
90. Devi PU, Bisht KS, Vinitha M. A comparative study of radioprotection by Ocimum flavonoids and synthetic aminothiol protectors in the mouse. Br J Radiol. 1998; 71:782-4.
91. Ganasoundari A, Devi PU, Rao BS. Enhancement of bone marrow radioprotection and reduction of WR-2721 toxicity by Ocimum sanctum. Mutat Res. 1998; 397:303-312.
92. Saija A, Scalese M, Lanza M, Marzillo D, Bonina F, Castelli F. Flavonoids as antioxidant agents: Importance of their interaction with biomembrane. Free Rad Biol Med.1995; 19: 481.
93. Kelm MA, Nair MG, Strasburg GM, DeWitt DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn. Phytomedicine 2000; 7(1):7-13.
94. Trevisan MT, Vasconcelos Silva MG, Pfundstein B, Spiegelhalder B, Owen RW. Characterization of the volatile pattern and antioxidant capacity of essential oils from different species of the genus Ocimum. J Agric Food Chem. 2006; 54:4378-4382.
95. Gupta S, Mediratta PK, Singh S, Sharma KK, Shukla R. Antidiabetic, antihypercholesterolaemic and antioxidant effect of Ocimum sanctum (Linn) seed oil. Indian J Exp Biol. 2006; 44:300-4.
96. Yanpallewar SU, Rai S, Kumar M, Acharya SB. Evaluation of antioxidant and neuroprotective effect of Ocimum sanctum on transient cerebral ischemia and long-term cerebral hypoperfusion. Pharmacol Biochem Behav. 2004; 79:155-64.
97. Mediratta PK, Dewan V, Bhattacharya SK, Gupta VS, Maiti PC, Sen P. Effect of Ocimum sanctum Linn on humoral immune responses. Indian J Med Res. 1988; 87:384-6.
98. Satayavanti GV, Raina MK, Sharma M. Medical plants of India published by ICMR, New Delhi, 1976.
99. Medrita PK, Dewan V, Bhattacharya SK, Gupta VS, Maiti PC, Sen P. Effect of Ocimum sanctum Linn. on humoral immune response. Ind J. Med. Res. 1987, 87:384.
100. Medrita PK, Sharma KK. Effect of essential oil of the leaves and fixed oil of the seeds of Ocimum sanctum on immune responses. J Med Aro Plant Sci. 2000; 22:694-700.
101. Mukherjee R, Dash PK, Ram GC. Immunotherapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis. Res Vet Sci. 2005; 79:37-43.
102. Mediratta PK, Sharma KK, Singh S. Evaluation of immunomodulatory potential of Ocimum sanctum seed oil and its possible mechanism of action. J Ethnopharmacol. 2002; 80:15-20.
103. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics. CA cancer J Clin. 2007; 57:43-66.
104. Aggarwal BB, Ichikawa H, Garodia P, Weerasinghe P, Sethi G, Bhatt ID, Pandey MK, Shishodia S, Nair MG. From traditional Ayurvedic medicine to modern medicine: identification of therapeutic targets for suppression of inflammation and cancer. Expert Opin Ther Targets. 2006; 10 (1): 87-118.
105. Kirtikar KR, Basu BD. Ocimum sanctum in Indian medicinal plants. 1933, 3, pp. 1965.
106. Carak Samhita (1000 B.C.) Chikitsa Sthana Section of Therapeutics, published by Ayurvedic Society of India, 1949; 3: 1278.
107. Nadkarni KM. Indian Materia Medica. Edn 3, Vol. I, Popular Prakashan, Mumbai, 1954, pp. 242-246.
108. Malviya BK, Gupta PL. Growth promoting properties of Ocimum sanctum. Indian J Pharmacy 1971; 33:126.
109. Singh TJ, Gupta PD, Khan SY, Misra KC. Preliminary pharmacological investigation of O. sanctum. India J Pharmacy 1970; 32: 93.
110. Krishnamurthy TR. Some pharmacological action of an extract of Ocimum sanctum. Indian J Physiol Pharmacol. 1959; 3: 92.
111. Vohra SB, Garg SK, Chowdhury RP. Effect of 6 indigenous plants on early pregnancy in albinorats. Indian J Pharmacy. 1968; 30:287.
112. Balta SK, Santhakumari G. The antifertility effect of O. sanctum and Hibiscus rasasinensis. Indian J med Res. 1971; 59: 777.
113. Palit G, Singh SP, Singh N, Kohli RP, Bhargava KP. Antiasthamatic potentials of O. sanctum and T. belerica. In Proc. 14th Convention Indian Coll. Aller. Appl. Immunol., 1980, pp-10.
114. Palit G, Singh SP, Singh N, Kohli RP, Bhargava KP. An experimental evaluation of Antiasthamatic plant drugs from the ancient ayurvedic medicine. Asp. Alter. Appl. Immunol. 1983; 16:36-41.
115. Siurin SA. Effects of essential oil on lipid peroxidation and lipid metabolism in patients with chronic bronchitis, Klin Med (Mosk). 1997; 75(10):43-5.
116. Singh N. A pharmaco-clinical evaluation of some Ayurvedic crude plant drugs as anti-stress agents and their usefulness in some stress diseases of man. Ann Nat Acad Ind Med. 1986; 2(1):14-26.
117. Das SK, Chandra A, Agarwal SS, Singh N. Ocimum sanctum (Tulsi) in the treatment of viral encephalitis (a prtelinary clinical trial). The Antiseptic 1983; 1-5.
118. Jalil A. Clinical trial of O. sanctum (Tulsi) in peptic ulcerand hyperacidity patients. J Res Ind Med. 1970; 4 (2):238-239.
119. Mediratta PK, Dewan V, Bhattacharya SK, Gupta VS, Malti PC, Sen P. Effect of O. sanctum Linn. on humoral immune response. Ind J Med Res. 1987; 87:384.
120. Singh N. A pharmaco- clinical evaluation of some ayurvedic crude plant drugs as anti stress agents and their usefulness in some stress disease of man.Ann. Nat Acad Ind Med. 1986; 2(1):14-26.
121. Singh N, Misra N. Stress disease and their possible remedy by anti-stress drugs (Adaptogens/ Staminators) of plant origin. Physiology of Human Performance. In Proc Nat Symp Physiol Hum Perfor. (Sawhney RC, Sridharan K and Selvamurthy W, eds) Publisher: Defence Institute of Physiology and Allied Science, Defence Research and Development Organization (DRDO), Govt. of India, Delhi, 1987, pp. 89-94.
122. Agarwal P, Rai V, Singh RB. Randomized placebo- controlled single blind trial of holy basil leaves in patients with non insulin-dependent diabetes mellitus. Int J Clin Pharmacol Ther. 1996; 34(9):406-409.
123. Puspangdan P, Sobti SN. Medicinal Properties of Ocimum (Tulsi) species and some recent investigation of their efficacy. Ind. Drugs 1977; 14 (11):207.
124. Patel VK, Bhatt HVK. Folklore therapeutics indigenous plants in periodontal disorder in India (review experimental and clinical approach). Int J Clin Pharmacol Ther Toxicol. 1988; 26 (4):176-184.
125. Adam US. Oceanite: A Maritime Union of India Publication, 1997; 197:37.
126. Singh N, Abbas SS. Effect of Ocimum sanctum (Tulsi) in sex-related chronic fatigue syndrome (SRCFS) in young Indians. J. Bio Chem Sci, 1995b, 14:184-187.
127. Sivarajan VV, Balachandran I. Tulsi. In: Ayurvedic Drugs and their Plant Sources. Oxford FBH Publishing Co. Pvt. Ltd., New Delhi, India, 1994, pp. 485- 486.
128. Gupta KC, Vishwanathan. A short note on Antitubercular substance from Ocimum sanctum. Antibiotic and Chemotherapy. 1955; 5:33.
129. Singh SP, Singh N. Experimental evaluation of adaptogenic properties of Ocimum sanctum. Ind. J. Pharmacol. 1978; 10:74.
130. Bhargava KP, Singh N. Anti-stress activity of Ocimum sanctum (Linn.). Ind. J. Med. Res.1981; 73:443-451.
131. Bhargava KP, Singh N. Comparative evaluation of anti-stress activity of Eleutherococcus senticosus, Panax ginseng and Ocimum sanctum. New Data on Eleutherococcus Moscow (U.S.S.R), 1984, pp. 181-89.
132. Bhalla M, Singh N. A clinical study of anticancer properties of Organic Ashwagandha, Tulsi and Wheat Grass. In the proceeding of the National Seminar- Brain Storming Session on Integrated Therapeutic Approach in the Management of Cancer, 26-27 march, 2011, Lucknow, pp.35.
133. Singh N, Hoette Y, Miller R. Tulsi ‘The Mother Medicine of Nature’ Ist Edn, International Institute of Herbal Medicine, Lucknow, 2002, pp. 29-42.
134. Singh N, Gilca M. Herbal Medicine – Science embraces tradition – a new insight into the ancient Ayurveda. Lambert Academic Publishing (Germany), 2010, pp. 315-322.
135. Jogetia GC, Devi PU, Singhatgeri MK, Singh N, Kohli, R.P. Proc. 56th Annual Session of National Academy of Sciences India, 1986, pp.40.
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“THERAPEUTIC POTENTIAL OF OCIMUM SANCTUM IN PREVENTION AND TREATMENT OF CANCER AND EXPOSURE TO RADIATION: AN OVERVIEW”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, Apr. 2012, pp. 97-104, https://doi.org/10.25004/IJPSDR.2012.040202.
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“THERAPEUTIC POTENTIAL OF OCIMUM SANCTUM IN PREVENTION AND TREATMENT OF CANCER AND EXPOSURE TO RADIATION: AN OVERVIEW”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, Apr. 2012, pp. 97-104, https://doi.org/10.25004/IJPSDR.2012.040202.
