IN-VITRO ANTIOXIDANT STUDIES OF PRITCHARDIA ARECINA
Abstract
In the present study, antioxidant activities of aqueous and methanolic fruit extracts of Pritchardia arecina were investigated. The antioxidant properties of the extracts were assessed using 1, 1,-diphenyl-2-picryl-hydrazyl (DPPH) assay and reducing power assay. Both the extracts exhibited significant reducing power which was dose dependent. The IC50 value in DPPH assay was found to be 18 and 27µg/ml for methanolic and aqueous extract respectively. Phytochemical analysis revealed the presence of tannins, steroids, amino acids and carbohydrates. The total phenolic content observed for aqueous and methanolic extracts were 25 and 40 mg gallic acid equivalent per gram of extract respectively.
Keywords:
Pritchardia arecina, antioxidant activities, total phenolic content, reducing power, DPPH assayDOI
https://doi.org/10.25004/IJPSDR.2012.040211References
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2. Halliwell B. Oxygen radicals and Disease process. Hardwood Academic Publisher, Netherlands. 1997, pp. 1-14.
3. Arnous A, Makris DP, Kefalas P. Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J Agric Food Chem. 2001; 49: 5736-5742.
4. Devasagayam TPA, Tilak JC, Boloor KK. Free radical and antioxidants in human health. Curr Stat Fut Pros JAPI. 2004; 53: 794-804.
5. Lu F, Foo LY. Toxicological aspects of food antioxidants. New York, Marcel Dekker, 1995.
6. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham J.B. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Res. 1995; 23: 375-383.
7. Riffle, Robert Lee, Paul Craft. An Encyclopedia of Cultivated Palms. Timber Press. 2003, pp. 419-422.
8. Jigna P, Nehal K, Sumitra C. Evaluation of antibacterial and phytochemical analysis of Bauhinia variegate L. bark. Afri J Biomed Res. 2006; 9(1): 53-56.
9. Singleton VL, Rossi JA. Colorimetry of total phenolic substances. US: American Chemical Society Symposium series 1965; 26: 47-70.
10. Braca A, Sortino C, Politi M. Anti-oxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol. 2002; 79: 379-381.
11. Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucoamine. Jpn J Nutr. 1986; 4: 307-315.
12. Lee J, Koo N, Min DB. Reactive oxygen species, aging and antioxidative neutraceuticals. Comp Rev Food Sci Food Safety. 2004; 3: 21-33.
13. Hatano T, Edamatsu R, Mori A, Fujita Y, Yasuhara E. Effect of interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chemical and Pharmaceutical Bulletin 1989; 37: 2016–2021.
14. Tanaka M, Kuei CW, Nagashima Y, Taguchi T. Application of antioxidative maillrad reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi. 1998; 54: 1409–1414.
15. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181: 1199-1201.
16. Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods & biological systems. Food Sci Tech Int. 2002; 8: 121-137.
17. Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defense system of various senescing leaves. J Agric Food Chem. 1995; 43: 1813-1815.
18. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soyabean oil in cyclodextrin emulsion. J Agric Food Chem. 1992; 40: 945-948.
19. Duh PD. Antioxidant activity of Budrock (Arctium laooa Linn.) its scavenging effect on free radical and active oxygen. J Am Oil Chem Soc 1998; 75: 455-461.
Published
01-04-2012
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“IN-VITRO ANTIOXIDANT STUDIES OF PRITCHARDIA ARECINA”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, Apr. 2012, pp. 140-2, https://doi.org/10.25004/IJPSDR.2012.040211.
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How to Cite
“IN-VITRO ANTIOXIDANT STUDIES OF PRITCHARDIA ARECINA”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, Apr. 2012, pp. 140-2, https://doi.org/10.25004/IJPSDR.2012.040211.
