EVALUATION OF DPPH RADICAL SCAVENGING ACTIVITY AND REDUCING POWER OF FOUR SELECTED MEDICINAL PLANTS AND THEIR COMBINATIONS
Abstract
Reactive oxygen species [ROS] cause oxidative damage to the tissues and protection from such damages are provided by endogenous and exogenous antioxidants. Plant based antioxidants are preferred due to the multiple mechanisms of actions and of the phytochemicals present in them. 80% alcoholic extract of leaves of Aloe vera, Bacopa monniera, Moringa oleifera and rhizome of Zingiber officinale was tested individually and in combination in equal proportion of each extract for DPPH scavenging activity and reducing power. The results indicate that the combination of the extract has better DPPH scavenging action and reducing power compared to the individual plant extract indicating synergistic and supra additive effect of phytochemicals present in the extract.
Keywords:
Reactive oxygen species [ROS], antioxidants, phytochemicals, DPPH scavenging activity, reducing power, synergistic effectsDOI
https://doi.org/10.25004/IJPSDR.2012.040212References
1. Yildrim A, Oktay M, Bilaloǧlu V, The antioxidant activity of leaves of Cydonia vulgaris. Turkish Journal of Medical Science 2001; 31:23-27.
2. Huang DH, Chen C, Lin C, Lin Y, Antioxidant and antiproliferative activities of water spinach (Ipomoea aquatica Forsk.) constituents. Botanical Bulletin of Academic Science 2005; 46:99-106.
3. Sun J, Chen Y, Li M, Ge Z. Role of antioxidant enzymes on ionizing radiation resistance. Free Radical Biology and Medicine 1998; 42:586-592.
4. Khilfi S, Hachimi E, Khalil A, Es-Safi A, Belahyam A, Tellal A, El Abbouyi A. In-vitro antioxidant properties of Salvia verbenaca. L. hydromethanolic extract. Indian Journal of Pharmacology 2006; 38:276-280.
5. Gulcin I, Alici HA, Cesur M. Determination of in- vitro antioxidant and radical scavenging activities of propofol. Pharmacology Bulletin 2005; 53:281-285.
6. Terao J, Piscula M, Yao MC. Protective effect of epicatechin, epicatechin gallate and quercetin on lipid peroxidation in phoshpolipid bilayers. Archives of Biochemistry and Biophysics 1994; 308:278-284.
7. Akinmoladun AC, Ibukun EO, Afor E, Akinrinlola BL, Onibon TR, Akinboboye AO, Obutor EM, Farombi EO. Chemical constituents and antioxidant activity of Alstonia boonei. African Journal of Biotechnology 2007; 6:1197-1201.
8. Hammam JH. Composition and applications of Aloe vera leaf gel. Molecules 2008; 13:1599-1616.
9. Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP. Bacopa monniera Linn. as an antioxidant mechanism of action. Indian Journal of Experimental Biology 1996; 34:523 -526.
10. Polassa K, Nirmala K. Ginger: its role in xenobiotic metabolism. Indian Council of Medical Research Bulletin 2003; 33:57-63.
11. Ghosh AK, Banerjee S, Mullick HI, Banerjee J. Zingiber officinale: A natural gold. International Journal of Pharma and Biosciences 2011; 2:283-294.
12. Sachan D, Jain SK, Singh N. In-vitro and in-vivo efficacy of Moringa oleifera plant constituents in urolithiasis as antilithiatic drug. International Journal of Pharmaceutical Sciences and Research 2011; 2:1638-1644.
13. Arun SL, Balkrishna Murthy AP, Sadasivan Pillai K. Screening of hepatoprotective effect of herbal mixture against CCl4 induced hepatotoxicity in swiss albino mice. Journal of Environmental Biology 2007; 28:201-207.
14. Folin O, Ciocalteu V. On tyrosine and tryptophan determination in proteins. Journal of Biology and Chemistry 1927; 27:627-650.
15. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 2002; 10:178-182.
16. Yermakov AI, Arasmov VV, Yarosh NP. Methods of biochemical analysis of plants. Agroponiztat Leningrad. 1987. (in Russian)
17. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology 1995; 28:25-30.
18. Senevirathne M, Kim S, Siriwardhana N, Hwan J, Lee K, Jeon Y. Antioxidant potential of Ecklonia cava on reactive oxygen species, metal chelating, reducing power and lipid peroxidation inhibition. Food Science and Technology 2006; 12:27-38.
19. Oyaizu M. Studies on products of browning reaction prepared from glucosamine. Japan Journal of Nutrition 1986; 44:307-315
20. Hong CY, Wang CP, Huang S, Hsu FC. The inhibitory effect of tannins on Lipid peroxidation of heart mitochondria. Journal of Pharmacy and Pharmacology 1995; 47:138-145.
21. Toda J, Khurana M, Ohinishi M. Effect of phenol carboxylic acid on superoxide anion and lipid peroxidation induced by superoxide anion. Planta Medica 1991; 57:8-10.
22. Faure M, Lissi E, Torres R, Videla LA. Antioxidant activities of lignans and flavonoids. Phytochemistry 1991; 29:3773-3775.
23. Dong Z. Molecular mechanisms and the chemopreventive effect of reveeretrol. Mutation Research 2003; 523:145-150.
24. Leung IK, Su Y, Chen R, Zang A, Huang Y, Chen ZY. The flavins in black and catechins in green tea are equally effective antioxidants. Journal of Nutrition 2000; 131:2248-2251.
25. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 2004; 85:231-237.
26. Shahidi, Wanasundara PD. Phenolic antioxidants. Critical Review of Food Science and Nutrition 1992; 32:67-103.
27. Hateno T, Edamatsu R, Mari A. Effects of interactions of tannins and co existing substances VI, effects of tannins and related polyphenols on superoxide anion radical and a DPPH radical. Chemistry and Pharmceutical Bulletin 1987; 37:2016-2021.
28. Hamberger M, Hastettman K .Bioactivity in plants: the link between phytochemistry and medicine .Phytochemistry 1991; 30:3864-3874.
29. Sabu MC, Kuttan R. Antidiabetic activity of medicinal plants and its relationship with antioxidant property. Journal of Ethnopharmacology 2002; 81:155-160.
30. Rajeshwar Y, Senthilkumar GP, Gupta M, Majumdar U. Studies on in-vitro antioxidant activities of methanol extract of Mucuna pruriens (Fabaceae) seeds. European Bulletin of Drug Research 2005; 13:31-39.
31. Joy KL, Kuttan R .Antioxidant activity of selected plant extracts. Amala Research Bulletin 1995; 15:68-71.
32. Premkumar K, Kavitha S, Sathiya TS, Ramesh A. Interactive effects of saffron with garlic and curcumin against cyclophosphamide induced genotoxicity in mice. Asia Pacific Journal of Clinical Nutrition 2004; 13:292-294.
33. Liu RH. Health benefits of fruits and vegetables are from additive and synergistic combinations of phytochemicals. American Journal of Clinical Nutrition 2003; 78: 5175-5205.
34. Liu RH. Potential synergy of phytochemicals in cancer prevention: Mechanism of action. Journal of Nutrition 2004; 134:34795-34855.
2. Huang DH, Chen C, Lin C, Lin Y, Antioxidant and antiproliferative activities of water spinach (Ipomoea aquatica Forsk.) constituents. Botanical Bulletin of Academic Science 2005; 46:99-106.
3. Sun J, Chen Y, Li M, Ge Z. Role of antioxidant enzymes on ionizing radiation resistance. Free Radical Biology and Medicine 1998; 42:586-592.
4. Khilfi S, Hachimi E, Khalil A, Es-Safi A, Belahyam A, Tellal A, El Abbouyi A. In-vitro antioxidant properties of Salvia verbenaca. L. hydromethanolic extract. Indian Journal of Pharmacology 2006; 38:276-280.
5. Gulcin I, Alici HA, Cesur M. Determination of in- vitro antioxidant and radical scavenging activities of propofol. Pharmacology Bulletin 2005; 53:281-285.
6. Terao J, Piscula M, Yao MC. Protective effect of epicatechin, epicatechin gallate and quercetin on lipid peroxidation in phoshpolipid bilayers. Archives of Biochemistry and Biophysics 1994; 308:278-284.
7. Akinmoladun AC, Ibukun EO, Afor E, Akinrinlola BL, Onibon TR, Akinboboye AO, Obutor EM, Farombi EO. Chemical constituents and antioxidant activity of Alstonia boonei. African Journal of Biotechnology 2007; 6:1197-1201.
8. Hammam JH. Composition and applications of Aloe vera leaf gel. Molecules 2008; 13:1599-1616.
9. Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP. Bacopa monniera Linn. as an antioxidant mechanism of action. Indian Journal of Experimental Biology 1996; 34:523 -526.
10. Polassa K, Nirmala K. Ginger: its role in xenobiotic metabolism. Indian Council of Medical Research Bulletin 2003; 33:57-63.
11. Ghosh AK, Banerjee S, Mullick HI, Banerjee J. Zingiber officinale: A natural gold. International Journal of Pharma and Biosciences 2011; 2:283-294.
12. Sachan D, Jain SK, Singh N. In-vitro and in-vivo efficacy of Moringa oleifera plant constituents in urolithiasis as antilithiatic drug. International Journal of Pharmaceutical Sciences and Research 2011; 2:1638-1644.
13. Arun SL, Balkrishna Murthy AP, Sadasivan Pillai K. Screening of hepatoprotective effect of herbal mixture against CCl4 induced hepatotoxicity in swiss albino mice. Journal of Environmental Biology 2007; 28:201-207.
14. Folin O, Ciocalteu V. On tyrosine and tryptophan determination in proteins. Journal of Biology and Chemistry 1927; 27:627-650.
15. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 2002; 10:178-182.
16. Yermakov AI, Arasmov VV, Yarosh NP. Methods of biochemical analysis of plants. Agroponiztat Leningrad. 1987. (in Russian)
17. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology 1995; 28:25-30.
18. Senevirathne M, Kim S, Siriwardhana N, Hwan J, Lee K, Jeon Y. Antioxidant potential of Ecklonia cava on reactive oxygen species, metal chelating, reducing power and lipid peroxidation inhibition. Food Science and Technology 2006; 12:27-38.
19. Oyaizu M. Studies on products of browning reaction prepared from glucosamine. Japan Journal of Nutrition 1986; 44:307-315
20. Hong CY, Wang CP, Huang S, Hsu FC. The inhibitory effect of tannins on Lipid peroxidation of heart mitochondria. Journal of Pharmacy and Pharmacology 1995; 47:138-145.
21. Toda J, Khurana M, Ohinishi M. Effect of phenol carboxylic acid on superoxide anion and lipid peroxidation induced by superoxide anion. Planta Medica 1991; 57:8-10.
22. Faure M, Lissi E, Torres R, Videla LA. Antioxidant activities of lignans and flavonoids. Phytochemistry 1991; 29:3773-3775.
23. Dong Z. Molecular mechanisms and the chemopreventive effect of reveeretrol. Mutation Research 2003; 523:145-150.
24. Leung IK, Su Y, Chen R, Zang A, Huang Y, Chen ZY. The flavins in black and catechins in green tea are equally effective antioxidants. Journal of Nutrition 2000; 131:2248-2251.
25. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 2004; 85:231-237.
26. Shahidi, Wanasundara PD. Phenolic antioxidants. Critical Review of Food Science and Nutrition 1992; 32:67-103.
27. Hateno T, Edamatsu R, Mari A. Effects of interactions of tannins and co existing substances VI, effects of tannins and related polyphenols on superoxide anion radical and a DPPH radical. Chemistry and Pharmceutical Bulletin 1987; 37:2016-2021.
28. Hamberger M, Hastettman K .Bioactivity in plants: the link between phytochemistry and medicine .Phytochemistry 1991; 30:3864-3874.
29. Sabu MC, Kuttan R. Antidiabetic activity of medicinal plants and its relationship with antioxidant property. Journal of Ethnopharmacology 2002; 81:155-160.
30. Rajeshwar Y, Senthilkumar GP, Gupta M, Majumdar U. Studies on in-vitro antioxidant activities of methanol extract of Mucuna pruriens (Fabaceae) seeds. European Bulletin of Drug Research 2005; 13:31-39.
31. Joy KL, Kuttan R .Antioxidant activity of selected plant extracts. Amala Research Bulletin 1995; 15:68-71.
32. Premkumar K, Kavitha S, Sathiya TS, Ramesh A. Interactive effects of saffron with garlic and curcumin against cyclophosphamide induced genotoxicity in mice. Asia Pacific Journal of Clinical Nutrition 2004; 13:292-294.
33. Liu RH. Health benefits of fruits and vegetables are from additive and synergistic combinations of phytochemicals. American Journal of Clinical Nutrition 2003; 78: 5175-5205.
34. Liu RH. Potential synergy of phytochemicals in cancer prevention: Mechanism of action. Journal of Nutrition 2004; 134:34795-34855.
Published
01-04-2012
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“EVALUATION OF DPPH RADICAL SCAVENGING ACTIVITY AND REDUCING POWER OF FOUR SELECTED MEDICINAL PLANTS AND THEIR COMBINATIONS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, Apr. 2012, pp. 143-6, https://doi.org/10.25004/IJPSDR.2012.040212.
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How to Cite
“EVALUATION OF DPPH RADICAL SCAVENGING ACTIVITY AND REDUCING POWER OF FOUR SELECTED MEDICINAL PLANTS AND THEIR COMBINATIONS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 4, no. 2, Apr. 2012, pp. 143-6, https://doi.org/10.25004/IJPSDR.2012.040212.
