THE INHIBITORY EFFECT OF METAL OXIDE NANOPARTICLES AGAINST POULTRY PATHOGENS
Abstract
The present study was aimed to investigate the antibacterial potential of metal oxide nanoparticles viz., Al2O3, Fe3O4, CeO2, ZrO2, and MgO against poultry pathogens viz., Klebsiella sp., E. coli, Staphylococcus sp. and Salmonella sp. The antibacterial activity of the metal oxide nanoparticles were assessed with well diffusion method. Various concentrations of nanoparticles were analyzed with minimum inhibitory concentration and minimum bactericidal concentration. Moreover, the potential nanoparticle was also tested with time kill assay. The ZrO2 showed maximum antibacterial activity against Salmonella sp. (15 ± 0.44 mm dia) followed by 12 ± 0.35 mm dia. against E. coli respectively. The MIC and MBC results revealed that, the ZrO2 nanoparticles inhibit the bacterial growth at a concentration of 2.5µg/ml against Salmonella sp. All the nanoparticles showed activity against all the tested pathogens. The time kill assay reveals that, the growth of the Salmonella sp. was inhibited by ZrO2 from the 1st h onwards. It is concluded from the present study that, the ZrO2 nanoparticles could be used as an effective antibacterial agent for the management of poultry systems.
Keywords:
Antibacterial activity, MIC, MBC, Metal oxide nanoparticles, Poultry pathogensDOI
https://doi.org/10.25004/IJPSDR.2012.040216References
2. Debnam AL, Jackson CR. Effect of growth promotant usage on Enterococci species on a poultry farm. Avian Dis. 2005; 49: 361-365.
3. FAO, FAOSTAT www.fao.org 2000. Statistical database of Food and Agriculture Organization of the United Nations, Rome Italy.
4. Ravikumar S, Gnanadesigan M, Suganthi P, Ramalakshmi R. Antibacterial potential of chosen mangrove plants against isolated urinary tract infectious bacterial pathogens. Int J Med Med Sci. 2010; 2(3): 94-99.
5. Ravikumar S, Nazar S, Nural Shiefa A, Abideen S. Antibacterial activity of traditional therapeutic coastal medicinal plants against some pathogen. J Environ Biol. 2005; 26: 383-386.
6. Ravikumar S, Ramanathan G, Subhakaran M, Jacob Inbaneson S. Antimicrobial compounds from marine halophytes for silkworm disease treatment. Int J Med Med Sci. 2009a; 1(5): 184-191.
7. Ravikumar S, Thajudeen T, Suganthi P, Jacob Inbeneson S, Vinothkumar T. Bioactive potential of Seagrass bacteria against human bacterial pathogens. J Environ Biol. 2009; 31: 387-389.
8. Oluwafemi F, Debiri F. Antimicrobial Effect of Phyllanthus amarus and Parquetina nigrescens on Salmonella typhi. Af J Biomed Res. 2008; 11: 215-219.
9. Hammond SM, Lambert 1978. Antimicrobial actions, Edward Arnld Ltd, London, pp. 8-9.
10. Pandey VS. Epidemiology and economics of village poultry production in Africa: Overview. Conference Proceedings, Village poultry production in Africa, Rabat, Morocco Pandey VS and Demey F. (Eds), 1992; pp. 124-128.
11. Aini I. Indigenous chicken production in South-east Asia, World's Poul Sci J. 1990; 46: 51-57.
12. Rezaei-Zarchi S, Javed A, Ghani MJ, Soufian S, Firouzabadi FB, Moghaddam AB, Mirjalili SH. Comparative Study of Antimicrobial Activities of TiO2 and CdO Nanoparticles against the Pathogenic Strain of Escherichia coli. Iran J Pathol. 2010; 5(2): 83-89.
13. Zhang H, Chen G. Potent antibacterial activities of Ag/TiO2 nanocomposite powders synthesized by a one-pot sol-gel method. Environ Sci Technol. 2009; 43(8):2905-2910.
14. Baker C, Pradhan A, Paktis L, Pochan DJ, Shah SI. Synthesis and antibacterial properties of silver nanoparticles. J Nanosci Technol. 2005; 5: 244-249
15. Martinez-Castanon GA, Nino-Martinez N, Martinez-Gutierrez F, Martinez Mendoza JR, Ruiz F. Synthesis and antibacterial activity of silver nanoparticles with different sizes. J Nanopart Res. 2008; 10: 1343-1348.
16. Li P, Li J, Wu C, Wu Q, Li J. Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnol. 2005; 16: 1912-1917.
17. Lin W, Yue-wern H, Xiao-Dong Z, Ma Y. Toxicity of Cerium Oxide Nanoparticles in Human Lung Cancer Cells. Int J Toxicol. 2006; 25(6): 451-457.
Published
Abstract Display: 331 
PDF Downloads: 394 
