In vivo and in vitro Antidiabetic Characterization of Nymphaea alba leaves
Abstract
The study was aimed to trace out antihyperglycemic potentials of Nymphaea alba leaves using in vitro and in vivo approaches. In, in vitro study, determination of IC50 of Nymphaea alba extracts was done using α-amylase and α-glucosidase inhibition assay. In α-amylase, Nymphaea alba methanol extract (NAME) exhibited maximum inhibition 56.77±1.23% at 125µg/ml in comparison to 66.7±0.94 % of standard acarbose. In α-glucosidase, NAME exhibits 59.89±0.92, while standard acarbose showed 70.31±1.25 % inhibition. The in vivo study was performed on diabetic rats, made diabetic by Streptozotocin. 200mg/kg and 400mg/kg NAME was administered orally, which significantly (
Keywords:
Nymphaea alba, Hyperglycemia, Antioxidant, Streptozotocin, in vitroDOI
https://doi.org/10.25004/IJPSDR.2020.120520References
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28. Feng, J,Yang, X-W, Wang, R-F. Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of aquilaria sinensis. Phytochemistry 2011; 72 (2-3): 242-247.
29. Rani, A,Kumar, S, Khar, RK. In vitro antidiabetic and hypolipidemic activity of selected medicinal plants.
30. Dhalwal, K,Deshpande, Y,Purohit, A, Kadam, S. Evaluation of the antioxidant activity of sida cordifolia. Pharmaceutical biology 2005; 43 (9): 754-761.
31. Nabavi, S,Ebrahimzadeh, M,Nabavi, S,Hamidinia, A, Bekhradnia, A. Determination of antioxidant activity, phenol and flavonoids content of parrotia persica mey. Pharmacologyonline 2008; 2 (9): 560-567.
32. Verma, A,Jana, GK,Sen, S,Chakraborty, R,Sachan, S, Mishra, A. Pharmacological evaluation of saraca indica leaves for central nervous system depressant activity in mice. J Pharm Sci Res 2010; 2 (6): 338-343.
33. Pandit, R,Phadke, A, Jagtap, A. Antidiabetic effect of ficus religiosa extract in streptozotocin-induced diabetic rats. Journal of ethnopharmacology 2010; 128 (2): 462-466.
34. Thippeswamy, B,Mishra, B,Veerapur, V, Gupta, G. Anxiolytic activity of nymphaea alba linn. In mice as experimental models of anxiety. Indian journal of pharmacology 2011; 43 (1): 50.
35. Barik, R,Jain, S,Qwatra, D,Joshi, A,Tripathi, GS, Goyal, R. Antidiabetic activity of aqueous root extract of ichnocarpus frutescens in streptozotocin-nicotinamide induced type-ii diabetes in rats. Indian Journal of Pharmacology 2008; 40 (1): 19.
36. Kaushik, P,Kaushik, D,Khokra, SL, Sharma, A. Antidiabetic activity of the plant abutilon indicum in streptozotocin-induced experimental diabetes in rats. Int. J. Pharmacog. Phytochem. Res 2010; 2 45-49.
37. Muruganandan, S,Srinivasan, K,Gupta, S,Gupta, P, Lal, J. Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. Journal of ethnopharmacology 2005; 97 (3): 497-501.
38. Aslan, M,Orhan, DD,Orhan, N,Sezik, E, Yesilada, E. In vivo antidiabetic and antioxidant potential of helichrysum plicatum ssp. Plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of ethnopharmacology 2007; 109 (1): 54-59.
39. Seedevi, P,Ganesan, AR,Moovendhan, M,Mohan, K,Sivasankar, P,Loganathan, S,Vairamani, S, Shanmugam, A. Anti-diabetic activity of crude polysaccharide and rhamnose-enriched polysaccharide from g. Lithophila on streptozotocin (stz)-induced in wistar rats. Scientific Reports 2020; 10 (1): 1-12.
40. Nayak, Y,Hillemane, V,Daroji, VK,Jayashree, B, Unnikrishnan, M. Antidiabetic activity of benzopyrone analogues in nicotinamide-streptozotocin induced type 2 diabetes in rats. The Scientific World Journal 2014; 2014
41. Humason, GL. Animal tissue techniques. Animal tissue techniques. 1962;
2. Kharroubi, AT, Darwish, HM. Diabetes mellitus: The epidemic of the century. World journal of diabetes 2015; 6 (6): 850.
3. Ahmed, AM. History of diabetes mellitus. Saudi medical journal 2002; 23 (4): 373-378.
4. Wang, Z,Zhao, X,Liu, X,Lu, W,Jia, S,Hong, T,Li, R,Zhang, H,Peng, L, Zhan, X. Anti-diabetic activity evaluation of a polysaccharide extracted from gynostemma pentaphyllum. International journal of biological macromolecules 2019; 126 209-214.
5. Maheshwari, RA,Khatri, K,Sailor, G, Balaraman, R. Antidiabetic activity of dibolin (a polyherbal formulation) in streptozotocin-nicotinamide induced type 2 diabetic rats. Int J Pharm Pharm Sci 2014; 2 893-897.
6. Verspohl, E. Novel pharmacological approaches to the treatment of type 2 diabetes. Pharmacological reviews 2012; 64 (2): 188-237.
7. Balunas, MJ, Kinghorn, AD. Drug discovery from medicinal plants. Life sciences 2005; 78 (5): 431-441.
8. Dixon, RA. Engineering of plant natural product pathways. Current opinion in plant biology 2005; 8 (3): 329-336.
9. Sabu, M, Kuttan, R. Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property. Journal of ethnopharmacology 2002; 81 (2): 155-160.
10. Ekor, M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in pharmacology 2014; 4 177.
11. Azadbakhta, M,Safapour, S,Ahmadi, A,Ghasemi, M, Shokrzadeh, M. Anti-diabetic effects of aqueous fruits extract of diospyros lotus l. On streptozotocin-induced diabetic rats and the possible morphologic changes in the liver, kidney and heart. Journal of Pharmacognosy and Phytotherapy 2010; 2 (2): 010-016.
12. Organization, WH. Who expert committee on diabetes mellitus [meeting held in geneva from 25 september to 1 october 1979]: Second report. 1980;
13. Tiwari, AK, Rao, JM. Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Current science 2002; 30-38.
14. Bose, A,Sahoo, M, Ray, SD. In vivo evaluation of anti-diarrheal activity of the rhizome of nymphaea alba (nymphaeaceae). Oriental Pharmacy and Experimental Medicine 2012; 12 (2): 129-134.
15. Nazir, S,Qureshi, M, Chat, O. Anti-tumor, anti-oxidant and anti-microbial potential of nymphaea alba and nymphaea mexicana flowers–a comparative study. Adv. Biomed. Pharma 2015; 2 196-204.
16. Karthikeyan, R. Anti-inflammatory activity of flowers of nymphaea alba by hrbc membrane stabilization method. Research in Plant Biology 2015; 5 (4):
17. Heslop-Harrison, Y. Nymphaea l. Journal of Ecology 1955; 43 (2): 719-734.
18. Lakshmi, T,Madhusudhanan, N, Rajendran, R. Nymphaea alba linn-an overview. Research journal of Pharmacy and Technology 2013; 6 (9): 974.
19. Sumlu, S,Atar, H, Khawar, K. Breaking seed dormancy of water lily (nymphaea alba l.) under in vitro conditons. Biotechnology & Biotechnological Equipment 2010; 24 (1): 1582-1586.
20. Kłosowski, S, Tomaszewicz, H. Habitat conditions of the nymphaeetum candidae miljan 1958 and nupharo-nymphaeetum albae tomaszewicz 1977 dominated by nymphaea alba. Acta Societatis Botanicorum Poloniae 1989; 58 (4): 613-624.
21. Bose, A,Ray, SD, Sahoo, M. Central depressant activity of ethanol extract of nymphaea alba rhizome in mice. Oriental Pharmacy and Experimental Medicine 2013; 13 (2): 159-164.
22. Rajagopal, K,Sasikala, K, Ragavan, B. Hypoglycemic and antihyperglycemic activity of nymphaea stellata flowers in normal and alloxan diabetic rats. Pharmaceutical biology 2008; 46 (9): 654-659.
23. Rajagopal, K, Sasikala, K. Antidiabetic activity of hydro-ethanolic extracts of nymphaea stellata flowers in normal and alloxan induced diabetic rats. Afr J Pharm Pharmacol 2008; 2 (8): 173-178.
24. Rahuja, N,Mishra, A,Gautam, S,Tamrakar, AK,Maurya, R,Jain, SK, Srivastava, AK. Antidiabetic activity in flowers of nymphaea rubra. Int J Pharm Sci Rev Res 2013; 22 (24): 121-133.
25. Council, NR: Guide for the care and use of laboratory animals; National Academies Press, 2010.
26. Ali, H,Houghton, P, Soumyanath, A. Α-amylase inhibitory activity of some malaysian plants used to treat diabetes; with particular reference to phyllanthus amarus. Journal of ethnopharmacology 2006; 107 (3): 449-455.
27. Sudha, P,Zinjarde, SS,Bhargava, SY, Kumar, AR. Potent α-amylase inhibitory activity of indian ayurvedic medicinal plants. BMC complementary and alternative medicine 2011; 11 (1): 5.
28. Feng, J,Yang, X-W, Wang, R-F. Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of aquilaria sinensis. Phytochemistry 2011; 72 (2-3): 242-247.
29. Rani, A,Kumar, S, Khar, RK. In vitro antidiabetic and hypolipidemic activity of selected medicinal plants.
30. Dhalwal, K,Deshpande, Y,Purohit, A, Kadam, S. Evaluation of the antioxidant activity of sida cordifolia. Pharmaceutical biology 2005; 43 (9): 754-761.
31. Nabavi, S,Ebrahimzadeh, M,Nabavi, S,Hamidinia, A, Bekhradnia, A. Determination of antioxidant activity, phenol and flavonoids content of parrotia persica mey. Pharmacologyonline 2008; 2 (9): 560-567.
32. Verma, A,Jana, GK,Sen, S,Chakraborty, R,Sachan, S, Mishra, A. Pharmacological evaluation of saraca indica leaves for central nervous system depressant activity in mice. J Pharm Sci Res 2010; 2 (6): 338-343.
33. Pandit, R,Phadke, A, Jagtap, A. Antidiabetic effect of ficus religiosa extract in streptozotocin-induced diabetic rats. Journal of ethnopharmacology 2010; 128 (2): 462-466.
34. Thippeswamy, B,Mishra, B,Veerapur, V, Gupta, G. Anxiolytic activity of nymphaea alba linn. In mice as experimental models of anxiety. Indian journal of pharmacology 2011; 43 (1): 50.
35. Barik, R,Jain, S,Qwatra, D,Joshi, A,Tripathi, GS, Goyal, R. Antidiabetic activity of aqueous root extract of ichnocarpus frutescens in streptozotocin-nicotinamide induced type-ii diabetes in rats. Indian Journal of Pharmacology 2008; 40 (1): 19.
36. Kaushik, P,Kaushik, D,Khokra, SL, Sharma, A. Antidiabetic activity of the plant abutilon indicum in streptozotocin-induced experimental diabetes in rats. Int. J. Pharmacog. Phytochem. Res 2010; 2 45-49.
37. Muruganandan, S,Srinivasan, K,Gupta, S,Gupta, P, Lal, J. Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. Journal of ethnopharmacology 2005; 97 (3): 497-501.
38. Aslan, M,Orhan, DD,Orhan, N,Sezik, E, Yesilada, E. In vivo antidiabetic and antioxidant potential of helichrysum plicatum ssp. Plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of ethnopharmacology 2007; 109 (1): 54-59.
39. Seedevi, P,Ganesan, AR,Moovendhan, M,Mohan, K,Sivasankar, P,Loganathan, S,Vairamani, S, Shanmugam, A. Anti-diabetic activity of crude polysaccharide and rhamnose-enriched polysaccharide from g. Lithophila on streptozotocin (stz)-induced in wistar rats. Scientific Reports 2020; 10 (1): 1-12.
40. Nayak, Y,Hillemane, V,Daroji, VK,Jayashree, B, Unnikrishnan, M. Antidiabetic activity of benzopyrone analogues in nicotinamide-streptozotocin induced type 2 diabetes in rats. The Scientific World Journal 2014; 2014
41. Humason, GL. Animal tissue techniques. Animal tissue techniques. 1962;
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30-09-2020
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“In Vivo and in Vitro Antidiabetic Characterization of Nymphaea Alba Leaves”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 12, no. 5, Sept. 2020, pp. 565-71, https://doi.org/10.25004/IJPSDR.2020.120520.
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How to Cite
“In Vivo and in Vitro Antidiabetic Characterization of Nymphaea Alba Leaves”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 12, no. 5, Sept. 2020, pp. 565-71, https://doi.org/10.25004/IJPSDR.2020.120520.
