Antihyperglycemic, Antioxidant and Hepatoprotective Properties of Smilax wightii A.DC. : an Endemic Plant of Western Ghats
Abstract
Phytotherapy is an inevitable companion of human civilization. Smilax wightii is an ethnomedicinal plant in Smilacaceae, with unexplored scientifically therapeutic potential. The antihyperglycemic, antioxidant and hepatoprotective capabilities of the methanolic extract of leaf, stem, rhizome and root of S. wightii were inspected in the present study. Hyperglycemia is a manifestation of the prevalent metabolic disorder, Type 2 Diabetes mellitus. Inhibitors of α –glucosidase and α – amylase could be efficiently employed in diabetes mellitus therapy as hypoglycemic agents. In the α –glucosidase and α – amylase inhibitory assays, root and rhizome extracts recorded better antihyperglycemic activity. DPPH radical scavenging activity and total antioxidant capacity were the parameters employed to determine the antioxidant activity. Hepatoprotectivity determines the capability of samples to safeguard the hepatocytes from damage. Novel hepatoprotective agents are in demand since the incidence of liver impairment is on a high among global population. The rhizome extract showed comparatively superior hepatoprotectivity followed by the leaf, stem and root extracts. Rhizome, at 100μg/ml guaranteed a cell viability percentage of 77.43 in the Chang liver cell line treated with Carbon tetrachloride. So the root and rhizome of S. wightii are the therapeutically significant plant parts with hypoglycemic, free radical scavenging and hepatoprotective potentialities.
Keywords:
Smilax wightii, α –glucosidase, α – amylase, hepatoprotective, antioxidantDOI
https://doi.org/10.25004/IJPSDR.2020.120601References
Adhikari BS, Babu MM, Saklani PL, Rawat GS. Medicinal Plants Diversity and their Conservation Status in Wildlife Institute of India (WII) Campus, Dehradun. Ethnobot Leaflets. 2010; 14:46–83.
Maheswari PU, Shalimol A, Arumugasamy K. Free Radical Scavenging Activity of Smilax wightii A. DC. ( Smilacaceae ), an Endemic Medicinal Plant from Western Ghats. Int J Herb Med. 2014; 2(2):106–8.
Devi VA, Arumugasamy KA, Shalimol A, Kumar RN, Udhayasankar MR, Kokilavani R. Anti-Inflammatory Activity of Smilax wightii fruit, Endemic A.DC.(Smilacaceaea) -An Endangered Medicinal Plant from the Nilgiris. JPBR. 2014; 2(2): 136-138
Maheswari PU, Shalimol A, Arumugasamy K, Udhayasankar U, Punitha D. Effect of methanolic extract of Smilax wightii A.Dc. on serum protein profile in streptozotocin induced diabetic rats. Int J PharmTech Res. 2014; 6(5):1870-1874.
Doss A, Vijayasanthi M. Evaluation of A-Amylase and A -Glucosidase Inhibitory Effects of Bioactive Compounds of Coccinia grandis (L.) Voigt Leaves. International Journal of Microbiological Research. 2016; 7(2):48–52.
Ann M A, David E J, James M F. Evaluation and prevention of diabetic neuropathy. Am Fam Physician. 2005; 71(11):2123-2130.
Kumar J, Kumar M. Medicinal Plants for Diabetes Mellitus : A Traditional Approach. Int Arch Appl Sci Technol. 2011; 2:37–46.
Kim SH, Hyun SH, Choung SY. Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. J Ethnopharmacol. 2006; 104(1–2):119–23.
Saliu J, Fapohunda O. The Antihyperglycemic, Hepatoprotective and Renoprotective Potentials of the Aqueous Extract of Costus lucanusianus on Streptozotocin-induced Diabetic Rats. J Appl Life Sci Int. 2016; 4(2):1–10.
Adebiyi OE, Olayemi FO, Ning-Hua T, Guang-Zhi Z. In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni-Suef Univ J Basic Appl Sci. 2017; 6(1):10–4.
Amat N, Upur H, Blažeković B. In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. J Ethnopharmacol. 2010; 131(2):478–84.
Kodavanti PR, Joshi UM, Young RA, Meydrech EF, Mehendale HM. Protection of hepatotoxic and lethal effects of CCl4 by partial hepatectomy. Toxicologic pathology. 1989; 17(3):494-505.
Rajagopal K, Sasikala K. Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan-induced diabetic rats. Singapore Med J. 2008; 49(2):137–41.
Mukherjee PK, Sahoo AK, Narayanan N, Kumar NS, Ponnusankar S. Lead finding from medicinal plants with hepatoprotective potentials. Expert Opin Drug Discov. 2009; 4(5):545–76.
De Smet PAGM. Herbal remedies. N Engl J Med. 2002; 347: 2046–2056
Worthington V. Maltase-a-glucosidase. In: Worthington Enzyme Manual. Freehold NJ. Worthington Biochemical Corp. 1996; 261 p.
Kwon YII, Vattem DA, Shetty K. Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pac J Clin Nutr. 2006; 15(1):107–18.
Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensmittel Wisenschaft and Technologie. 1995; 28(1):25-30
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2):337–41.
Marques SS, Magalhães LM, Tóth IV, Segundo MA. Insights on antioxidant assays for biological samples based on the reduction of copper complexes-The importance of analytical conditions. Int J Mol Sci. 2014; 15(7):11387–11402.
Nair SS, Kavrekar V, Mishra A. In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol. 2013; 3(1):128–32.
Subramanian R, Asmawi MZ, Sadikun A. In vitro α-glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochim Pol. 2008; 55(2):391–8.
Firdous SM. Phytochemicals for treatment of diabetes. EXCLI Journal. 2014; 13:451-453
Kumar CH, Ramesh A, Kumar JS, Ishaq BM. A review on hepatoprotective activity of medicinal plants. International Journal of Pharmaceutical sciences and research. 2011; 2(3):501.
Flora KD, Rosen HR, Benner KG. The use of naturopathic remedies for chronic liver disease. Am J Gastroenterol. 1996; 91:2654–2655.
Handa SS. Natural products and plants as liver protecting drugs. Fitoterapia. 1986; 57(5): 307-51.
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