FORMULATION AND OPTIMIZATION OF GUGGUL LIPID PHYTOSOMAL GEL OF THYMOQUINONE USING 22 FACTORIAL DESIGN
Abstract
Thymoquinone is a bioactive principal constituent of Nigella Sativa. Thymoquinone is hydrophobic, having low water solubility and less permeability. The study aimed to formulate and optimize Thymoquinone loaded phytosomes using guggul lipid by thin-film method to enhance permeation and stability. 2 factor 2 level design (22) using SigmaTech software was employed contour plots were used to predict the responses. The two independent variables used were: the amount of guggul lipid (X1), amount of cholesterol (X2); and the responses included entrapment efficiency (Y1), in-vitro drug release (Y2), and particle size (Y3). The optimized formulation of Thymoquinone phytosomes was incorporated into Carbopol 934 gel base and 0.4% thymoquinone phytosomal gel was prepared. The phytosomal gel was evaluated. The stability study was performed, and phytosomal gel formulation was found stable at 4°C for 45 days.
Keywords:
Thymoquinone, guggul lipid, optimization, factorial design, sigmatech software.DOI
https://doi.org/10.25004/IJPSDR.2021.130402References
Allen LV, Popovich NG and Ansel HC. Ansel’s Pharmaceutical dosage forms and drug delivery systems. Lippincott Williams & Wilkins 2011;9
Nikalje AP, Tiwari S. Ethosomes: A novel tool for transdermal drug delivery. Int J Res Pharm Sci. 2012; 2:1-20.
Manach C, Scalbert A, Morand C. Polyphenols food sources and bioavailability. Am J Clin Nutr. 2004; 79:727-747.
Bombardelli E, Curri SB, et al. Complexes between phospholipids and vegetal derivatives of biological interest. Fitoterapia 1989; 60:1-9.
Lu M, Qiu Q, et.al. Phyto-phospholipid complexes (phytosomes): A novel strategy to improve the bioavailability of active constituents. Asian J. Pharm. Sci. 2019; 14:265-274.
Pawar HA, Bhangale BD. Phytosome as a novel biomedicine: A microencapsulated drug delivery system. J Bioanal Biomed. 2015; 7: 6-12.
Ahmad S, Beg ZH. Hypolipidemic and antioxidant activities of Thymoquinone and Limonene in atherogenic suspension fed rats. Food Chemistry. 2013; 138:1116–1124.
Khader M, Peter M. Thymoquinone: an emerging natural drug with a wide range of medical applications. Iran J Basic Med Sci. 2014; 17: 950–957.
Jain A. Synergistic and sustained anti-inflammatory activity of guggul withthe Ibuprofen: A preliminary study. Int. J. Pharma. Biosci. 2010; 1: 2-8.
Vane J, Botting R. Inflammation and mechanism of action of anti inflammatory drugs. FASEB J 1987; 1: 89-96.
Dave V, Yadav R, Gupta S, Sharma S. Guggulosomes: A herbal approach for enhanced topical delivery of Phenylbutazone. Future J Pharm Sci. 2017; 3:23-32.
Jain A, Jain SK. Formulation and optimization of Temozolomide nanoparticles by 3 factor 2 level factorial design. Biomatter. 2013; 3.
Two-level factorial designs. Handbook of chemometrics and qualimetrics: Part A, 1988; 659–682. doi:10.1016/s0922- 3487(97)80052-4
Singh R, Narke R. Preparation and evaluation of phytosome of Lawsone. Int J Pharm Sci Res. 2015; 33:5217-5226.
Das MK, Kalita B. Design and evaluation of phyto-phospholipid complexes (phytosomes) of Rutin for transdermal application. J App Pharm Sci. 2014; 4:51-57.
Demir B, Barlas FB et al. Gold nanoparticle loaded phytosomal systems: synthesis, characterization and in vitro investigations. RSC Advances. 2014; 4:34687-34695.
Gaur P, Mishra S, and Aeri V. Formulation and evaluation of Guggul Lipid nanovesicles for transdermal delivery of Aceclofenac. The Scientific World Journal. 2014; 1-10.
Joshua MJ, Anilkumar A, Cu V, Vasudevan D, Surendran S. formulation and evaluation of antiaging phytosomal gel. Asian J Pharm Clin Res. 2018; 11: 409-423.
Rajashekhar K, Sundari P, Srinivas P. Development of a topical phytosomal gel of woodfordia fruticosa’, World J Pharm Pharm Sci. 2010; 4:919-932.
Mannan A, Begum S, Rasheed A. Formulation, development and evaluation of phytosomal gel of Thymoquinone, Int. J. Pharm. Biol. Sci. 2019; 9:419-431.
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