STUDY OF HYPOGLYCEMIC ACTIVITY OF AQUEOUS EXTRACT OF LEUCAS INDICA LINN. AERIAL PARTS ON STREPTOZOTOCIN INDUCED DIABETIC RATS
Abstract
The present study was designed to evaluate the hypoglycemic activity of the aqueous extract of Leucas indica Linn. on streptozotocin induced diabetic rats. The extract showed a significant dose depended (200 and 400 mg/kg b.w, orally) reduction in fasting blood glucose level, comparing with reference drug, glibenclamide (0.5 mg/kg b.w, orally). In addition, the changes in body weight, analysis of serum biochemical parameters like lipid profile, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, total proteins, bilirubin, urea and alkaline phosphatase were assessed for each group of animals and the significant differences were observed when compared with control groups. Finally, in case of histopathological investigation of pancreas, liver and kidney from each group of rats showed a significant protection and regeneration of earlier streptozotocin induced cellular necrosis. This finding proves that the extract exhibits sufficient hypoglycemic activity along with improves streptozotocin induced negative body weight as well as normalize serum biochemical parameters and protect from cellular necrosis significantly.
Keywords:
Leucas indica, Aqueous Extract, Hypoglycemic, Streptozotocin, Biochemical Parameters, HistopathologyDOI
https://doi.org/10.25004/IJPSDR.2013.050203References
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18. Eidi A, Eidi M, Shahmohammadi P, Mozaffarian V, Rustaiyan A, Mazooji A. Antidiabetic Effect of Salvia verticillata L. Aerial Parts in Normal and Streptozotocin-induced Diabetic Rats. International Journal of Pharmacology. 2011; 7(1): 66-73.
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22. Kananna VS, Roh DD, Kirschenbaum MA. Hyperlipidemia and Kidney disease: Concept derived from histopathology and cell biology of glomerulus. Histopathol. 1998; 13: 169-79.
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24. Robert JN, Els van N, Danny EC van H, Petra GB, Klaske van N, Paul AM, van L. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74 (4): 418-425.
2. Nissen SE, Wolski K. Effect Rosiglitazone on the risk of myocardial infarction and death from cardiovascular causes. N. Eng. Med. 2007; 356:2457-2471.
3. Gupta RK, Kesari AN, Wata G, Murthy PS, Chandra R, Maithal K, Tandon V. Hypoglycemic and antidiabetic effect of aqueous extract of leaves of Annona squamosal (L.) in experimental animal. Current Science 2005; 88: 1244-1254.
4. The WHO expert committee on Diabetes Mellitus. World Health Organization, 1980; Geneva: Technical report series 646.
5. Ivorra MD, Paya M, Villar A. A review of natural products and plants as potential antidiabetic drugs. J. Ethnopharmacol. 1989; 27: 243-275.
6. Ramani R, Karra HB, Boddupalli BM, Anisetti RN, Banji D. Pharmacognostical, Phytochemical and Anthelmintic Evaluation of Leucas indica (L). Pharmacognosy Journal 2009; 2(10):317-323.
7. Madhava CK, Sivaji K, Tulasirao K. Flowering plants of Chittor District, Andhra Pradesh, (Students offset printers, Tirupati) India, 2008, pp. 277.
8. Mostafa M, Nahar N, Mosihuzzaman M, Makhmoor T, Choudhary MI, Rahman AU. Free radical scavenging phenylethanoid glycosides from Leucas indica Linn. Nat Prod Res. 2007; 21(4):354-61.
9. Saha K, Mukharjee PK, Das J, Pal M, Saha BP. Wound healing activity of Leucas indica Ress. J. Ethanopharmacolgy. 1997; 56(2):139-44.
10. Harborne JB. Phytochemistry. Academic Press, London, 1993; pp. 89-131.
11. Trease GE, Evans WC. Pharmacology, 15th Edn. Saunders Publishers, London, 2002; pp. 42-44, 221-229, 246-249, 303-306, 331-332, 391-393.
12. Litchfield JT, Wilcoxon F. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 1949; 96: 99-133.
13. Babu V, Gangadevi Y, Subramaniam A. Anti-hyperglycemic activity of ethanol Cassia kleinii leaf extract in glucose fed normal rats and alloxan induced diabetic rats. Indian J Pharmacol. 2002 ; 34 : 409-415.
14. Fiky El FK, Abou-Karam MA, Afify E.A. Effect of Luffa aegyptiaca (seeds) and Carissa edulis (leaves) extract on blood glucose level of normal and streptozotocin diabetic rats. J. Ethnopharm. 1996; 50 : 43-47.
15. Datusalia AK, Dora CP, Sharma S. Acute and chronic hypoglycemic activity of Sida tiagh fruit in N-5 Streptozotocin diabetic rats. Acta Poloniac Pharmaceutica-Drug research 2012; 69: 699-706.
16. Parthasarathy R, Liavarasan R, Karrunakaran CM. Antidiabetic activity of Thespesis populnea bark and leaf extract against steptozotocin induced diabetic rats. International Journal of Phar Tech Research 2009; 4: 1069-1072.
17. Van HH, Baumans V, Brandt CJWM, Hesp APM, Sturkenboom JH, Van LHA, Van TG, Beynen AC. Orbital sinus blood sampling in rats as performed by different animal technicians: the influence of technique and expertise. Laboratory Animals 1998; 32: 377-386.
18. Eidi A, Eidi M, Shahmohammadi P, Mozaffarian V, Rustaiyan A, Mazooji A. Antidiabetic Effect of Salvia verticillata L. Aerial Parts in Normal and Streptozotocin-induced Diabetic Rats. International Journal of Pharmacology. 2011; 7(1): 66-73.
19. Saha P, Bala A, Kar B, Naskar S, Mazumder UK, Haldar PK, Gupta M. Antidiabetic Activity of Cucurbita maxima Aerial Parts. Research Journal of Medicinal Plant 2011; 5(5): 577-586.
20. Ali L, Azad Khan AK, Mamun MIR, Mosihuzzaman M, Nahar N, Nur-E-Alan M, Rokeya B. Studies on the hypoglycemic effect of fruits pulp, seed and whole plant of Momordica charantia on normal and diabetic model rats. Planta Medica 1993; 59: 408-412.
21. Burcelain R, Eddoouks M, Maury J, Kande J, Assan R, Girard J. Excessive glucose production rather than insulin resistance accounts for hypoglycaemia in recent-onset diabetic rats. Diabetologia 1995; 38: 283-90.
22. Kananna VS, Roh DD, Kirschenbaum MA. Hyperlipidemia and Kidney disease: Concept derived from histopathology and cell biology of glomerulus. Histopathol. 1998; 13: 169-79.
23. Jung HW, Jung JK, Ramaliangam M, Yoon CH, Park YK. Anti-diabetic effect of Wen-pi-tang-Hab-Wu-ling-san extract in streptozotocin-induced diabetic rats. Indian Journal of Pharmacology 2002; 44 (1): 97-102.
24. Robert JN, Els van N, Danny EC van H, Petra GB, Klaske van N, Paul AM, van L. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74 (4): 418-425.
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01-04-2013
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“STUDY OF HYPOGLYCEMIC ACTIVITY OF AQUEOUS EXTRACT OF LEUCAS INDICA LINN. AERIAL PARTS ON STREPTOZOTOCIN INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 5, no. 2, Apr. 2013, pp. 50-55, https://doi.org/10.25004/IJPSDR.2013.050203.
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How to Cite
“STUDY OF HYPOGLYCEMIC ACTIVITY OF AQUEOUS EXTRACT OF LEUCAS INDICA LINN. AERIAL PARTS ON STREPTOZOTOCIN INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 5, no. 2, Apr. 2013, pp. 50-55, https://doi.org/10.25004/IJPSDR.2013.050203.
