ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACTS OF BARK OF ZANTHOXYLUM ARMATUM DC IN PARACETAMOL-INDUCED HEPATOTOXICITY
Abstract
The scientific evaluation of medicinal plants used in the preparation of folk remedies has provided modern medicine with effective pharmaceuticals for the treatment of diseases. The ethanolic extract of Zanthoxylum armatum DC bark was investigated for its hepatoprotective and antioxidant effects on Paracetamol (500 mg/kg) induced acute liver damage in Wistar albino rats. Hepatoprotection activity was measured by using biochemical parameters such as serum glutamate oxalate transaminase and se-rum Glutamate Pyruvate Transaminase (SGOT and SGPT), alkaline phosphatase (ALP), bilirubin and total protein. The ethanolic extract of Zanthoxylum armatum DC(EZA) at the doses of (100 mg/kg ,200 mg/Kg and 400 mg/Kg) produced significant hepatoprotective effect by decreasing the activity of serum enzymes, bilirubin and lipid peroxidation while it significantly increased the levels of glutathione (GSH), catalyse (CAT) and super oxide dismutase (SOD) in a dose dependant manner. The effects of EZA were comparable to that of standard drug silymarin. These results suggest that EZA may have potential therapeutic value in the treatment of some liver disorders, probably by its antioxidative effect on hepatocytes.
Keywords:
Zanthoxylum armatum DC, Hepatoprotective effect, Antioxidants, Paracetamol, SilymarinDOI
https://doi.org/10.25004/IJPSDR.2013.050308References
2. Wolf PL. Biochemical diagnosis of liver diseases. Indian Journal of Clinical Biochemistry 1999; 14:59-90.
3. Ayurvedic Pharmacopoeia of India, Part 1. New Delhi: Government of India; 2001; 2:168-171.
4. Gogte VM. Ayurvedic Pharmacology and Therapeutic uses of Medicinal Plants (Dravyagunavignyan), 1st Eng. ed. Bharatiya Vidya Bhavan, Mumbai, 2000, pp. 644-645.
5. Kokate SD, Venkatachalam SR, Hassarajani SA. Zanthoxylum armatum extract as mosquito larvicide. Proceedings of the National Academy of Sciences, India. Section B—Biological Sciences. Allahabad: National Academy of Sciences, India 71B 2001; 229-232.
6. Kumar S, Müller K. Inhibition of keratinocyte growth by different Nepalese zanthoxylum species. Phytother Res. 1999; 13 (3):214-7.
7. Nadkarni AK. Indian Materia Medica, vol. 1, Bombay Popular Prakashan, 2002, pp.569-570
8. Kalia N, Singh B, Sood R. A new amide from zanthoxylum armatum. J Nat Prod. 1999; 62 (2):311-2.
9. Ranawat L, Bhatt J, Patel J. Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylum armatum DC in CCl4 induced hepatic damage in rats J Ethnopharmacol 2010;127(3) : 777-780.
10. Larson AM, Polson J, Fontana RJ. Acetaminophen-induced acute liver failure: results of a United States multicenter, prospective study. Hepatology. 2005; 42:1364–1372.
11. Black M. Acetaminophen hepatotoxicity. Gastroenterology.1980; 78:382-392.
12. Makin AJ, Williams R. Acetaminophen-induced hepatotoxicity: predisposing factors and treatments. Adv Intern Med. 1997; 42:453-483.
13. Udupa V, Kulkarni SK, Rafiqs M.Venkataranganna MV, Mitra SK. Effect of hd-03 on levels of various enzymes in paracetamol induced liver damage in rats. Indian J Pharmacol 2000; 32: 361-364.
14. Shenoy A, Somayaji S N, Bairy KL, Hepatoprotective Effects Of Ginkgo Biloba Against Carbon Tetrachloride Induced Hepatic Injury In Rats. Indian J Pharmacol 2001; 33: 260-266.
15. Moron MJ, Diperre JW, Mannerv KB. Levels of glutathione, Glutathione reductase and Glutathione-s-transferase activities in rat lungs and liver. Biochem Biophys. Acta. 1979; 582:67-71.
16. Slater TF, Sawyer BC. The stimulatory effects of carbon tetrachloride on peroxidative reactions in rat liver fractions in vitro. Interaction sites in the endoplasmic reticulum. Biochem. J. 1971; 123:815–821.
17. Misra HP, Fridovich I. The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase. Journal of Biological Chemistry 1972; 247(10): 3170.
18. Aebi H. Catalase. In: Bergmeyer, editor. Mehtod in enzymatic analysis, New York: Academic Press; 1974; 2:674-84.
19. Holt M P,Cynthia Ju. Mechanisms of Drug-Induced Liver Injury. The AAPS Journal 2006; 8 (1):E48-E54
20. Jollow DJ, Mitchell JR, Potter WZ, Davis DC, Gillette JR, Brodie BB. Acetaminophen induced hepatic necrosis: (II) Role of covalent binding in-vivo. J Pharmacol Exp Ther 1973; 187:195.
21. Muriel P, Garciapina T, Perez-Alvarez V, Murelle M. Silymarin protect against Paracetamol-induced lipid peroxidation and liver damage. J Appl Toxicol 1992; 12:439-42.
22. Donnelly PJ, Walker RM, Racz WJ. Inhibition of mitochondrial respiration in vivo is an early event in acetaminophen-induced hepatotoxicity. Arch Toxicol 1994; 68:110-118.
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