ANTIOXIDANT AND NEPHROPROTECTIVE ACTIVITY OF FLAVONOID RICH FRACTION OF ALPHONSEA SCLEROCARPA Thw

Authors

  • SUDHEER KUMAR DOKUPARTHI School of Health Science and Pharmacy, Career Point University, Kota, Rajasthan, India
  • T. RAMA MOHAN REDDY School of Health Science and Pharmacy, Career Point University, Kota, Rajasthan, India

Abstract

The kidney is one of the crucial organs to maintain proper homeostasis in the body, and develop nephroprotective agents to retain kidney health and an absolute requisite. Since plant-based medicine gained importance due to fewer side effects and more safety, a herbal approach to develop nephroprotective agents is still necessary. Our present investigation is aimed to screen the antioxidant and nephroprotective activity of Alphonsea sclerocarpa leaves. Wistar rats were used in the current paracetamol-induced nephrotoxicity model through standard experimental procedures. N-acetyl cysteine (NAC) is taken as standard, the flavonoid-rich fraction of A. sclerocarpa was given to the animals in two doses (200 and 400 mg/kg body weight) for seven days. After the treatment, blood samples were collected from all the animals and sacrificed for histopathological studies. To support this investigation, phytochemical screening, total phenolic, flavonoid, tannin contents were also determined. The antioxidant activity studies by DPPH assay, NO free radical scavenging assay, and total antioxidant assay were also screened using standard protocols. Results unveil that the plant is rich in polyphenolic contents, and the flavonoid-rich fraction has statistically significant (P<0.001) nephroprotective activity in a dose-dependent manner compared to the standard N-acetyl cysteine. The biochemical and histological studies were also conducted in connection with the titled activity and found significant to improve the abnormal blood parameters and enzymatic and non-enzymatic antioxidants in the kidney tissue. To wrap up, all the biochemical, behavioral, and histological data declared that the flavonoid-rich fraction of A. sclerocarpa is more potent among all the extracts to protect against oxidative stress and subsequent improvement of the physiological parameters of the kidney. The antioxidant property of the phytochemicals present in the extract may be responsible for the affirmed activity.

Keywords:

A. sclerocarpa, antioxidant, total phenolic, total flavonoid, total tannins, nephroprotective.

DOI

https://doi.org/10.25004/IJPSDR.2021.130404

References

Suman Joshi DS, Amgoth C, Surya Narayana S, Padmavathi CH, Madhavi J, Krishna Satya A. Antioxidant and anticancer activities of an Aporphine alkaloid isolated from Alphonsea sclerocarpa. The Journal of Phytopharmacology. 2018; 7(1):51-55

Bakri YM, Talip MA, Abdul Azziz SS. A Mini Review on Alphonsea sp. (Annonaceae): Traditional uses, Biological Activities and Phytochemistry. J App Pharm Sci. 2017; 7 (10):200-203

Tacić D, Wannigama GP, Cassels BK, Cavé A. Alklaloids of Alphonsea sclerocarpa. Journal of Natural Products. 1987; 50(3):518-519

Prasad DN. Antioxidant activity of Alphonsea sclerocarpa bark. Research Journal of Pharmacology and Pharmacodynamics. 2009; 1(2):66-69

DSD SJ, Rao V. Phytochemical screening and evaluation of antioxidant, antibacterial and antifungal activity of medicinal plant Alphonsea sclerocarpa Thaw. Journal of Pharmacognosy and Phytochemistry. 2017; 6(4):1280-1286

Doddapaneni SJ, Amgoth C, Kalle AM, Suryadevara SN, Alapati KS. Antimicrobial and anticancer activity of AgNPs coated with Alphonsea sclerocarpa extract. 3Biotech. 2018; 8(3):156

Chandrasekar R, Jayanth PC, and Niranjan Babu M. Protective effect of ethanolic leaf extract of Alphonsea sclerocarpa against ethylene glycol induced urolithiasis in rats. Indian Journal of Natural Products and Resources. 2019; 10(4):252-258

Sadhna Jain, Punita Saxena, Heena Lamba. Effect of Sprouting Time on Antioxidant Properties of Trigonella foenum graecum (Fenugreek) Seeds Available in Delhi-NCR Region. 2017; 9(5):33-37

Aryal Sushant et al. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants (Basel, Switzerland). 2019; 8(4):96

Agrawal S, Kulkarni GT, Sharma VN. A comparative study on the antioxidant activity of methanolic extracts of Terminalia paniculata and Madhuca longifolia. Free Rad Antiox. 2011; 1:62-68

Rita, Rajesh Kumar Bajaj, Jyotika Dhankar, Dinesh Babu Nalla. Assessment of Strawberry Polyphenols Aqueous Extract for Major Compositional and Biofunctional Attributes. International Journal of Current Research and Review. 2018; 10(22):1-6

Neeraj, John Pramod, Sheena Singh, Joydeep Singh. Role of free radicals and antioxidants in human health and disease. International Journal of Current Research and Review. 2013; 05(19):14-22

Lee YH, Choo C, Watawana MI, Jayawardena N, Waisundara VY. An appraisal of eighteen commonly consumed edible plants as functional food based on their antioxidant and starch hydrolase inhibitory activities. J Sci Food Agric. 2015; 95:2956-2964

Bektur NE, Sahin E, Baycu C, Unver G. Protective effects of silymarin against acetaminophen-induced hepatotoxicity and nephrotoxicity in mice. Toxicol. Ind. Health. 2016; 32(4):589–600

Neelima S, Dwarakanadha Reddy P, Kothapalli Bannoth CS. Nephroprotective activity of Annona Squamosa leaves against paracetamol-induced nephrotoxicity in rats: in vitro and In vivo experiments. Futur J Pharm Sci. 2020; 6(131):1-8

Al-Naimi, Marwa S et al. Nephrotoxicity: Role and significance of renal biomarkers in the early detection of acute renal injury. Journal of advanced pharmaceutical technology & research. 2019; 10(3):95-99.

Miceli N, Buongiorno LP, Celi MG, Cacciola F, Dugo P, Donato P, Taviano, MF. Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficate Link. (Fabaceae) leaves extract. Natural Product Research. 2015; 30(11):1229– 1239

Sudheer Kumar Dokuparthi et al. Skeletal Muscle Relaxant Activity of Bougainvillea glabra Leaves in Swiss Albino Mice. Indo American Journal of Pharmaceutical Research. 2018; 8(03):390- 393

Harbone JB. Phytochemical methods: A guide to modern techniques of plant analysis. London: Chapman and Hall Ltd., 1973.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic. 1965; 16:144-158

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10:178-182

Broadhurst RB, jones WT. Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture. 1978; 48(3):788–794

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181:1199-1200

Rajasekaran SR. Anandan. Anti-oxidantant and phytochemical screening of Acalypha communis by HPTLC finger printing method. International Journal of Current Research and Review. 2016; 08(14):33-40

Prieto P, Pineda M, M Aguilar. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999; 269:337-341

Dokuparthi SK, Md Khan A, Anusha A, Bee M, Shaibaz, Shahajeb S, Sunil Kumar K, Suthakaran R. Acute oral toxicity study of Tectona grandis Linn. methanolic seed extract in albino mice. J Phytopharmacol. 2017;6(2):183-185

Hussain T, Gupta RK, Sweety K, Eswaran B, Vijayakumar M, Rao CV. Nephroprotective activity of Solanum xanthocarpum fruit extract against gentamicin-induced nephrotoxicity and renal dysfunction in experimental rodents. Asian Pac J Trop Med. 2012; 5(9):686-691

Dogukan Canayakin et al. Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa, Pharmaceutical Biology. 2016; 54(10): 2082-2091

El-Boshy ME et al. The remedial effect of Thymus vulgaris extract against lead toxicity-induced oxidative stress, hepatorenal damage, immunosuppression, and hematological disorders in rats. Environ. Sci. Pollut. Res. 2019; 26(12):1–11

Umamaheswari M, Chatterjee TK: In vitro antioxidant activities of the fractions of Coccinnia grandis L. leaf extract. Afr J Trad Compl Altern Med. 2008; 5:61-73

Krishnaiah D, Sarbatly R, Nithyanandam RR: A review of the antioxidant potential of medicinal plant species. Food Bioprod Process. 2011; 89:217-233.

Published

30-07-2021
Statistics
Abstract Display: 592
PDF Downloads: 483
Dimension Badge

How to Cite

“ANTIOXIDANT AND NEPHROPROTECTIVE ACTIVITY OF FLAVONOID RICH FRACTION OF ALPHONSEA SCLEROCARPA Thw”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 13, no. 4, July 2021, pp. 384-9, https://doi.org/10.25004/IJPSDR.2021.130404.

Issue

Section

Research Article

How to Cite

“ANTIOXIDANT AND NEPHROPROTECTIVE ACTIVITY OF FLAVONOID RICH FRACTION OF ALPHONSEA SCLEROCARPA Thw”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 13, no. 4, July 2021, pp. 384-9, https://doi.org/10.25004/IJPSDR.2021.130404.

Similar Articles

1-10 of 280

You may also start an advanced similarity search for this article.