NEUROPROTECTIVE EVALUATION OF LEAF EXTRACT OF DALBERGIA SISSOO IN 3-NITROPROPIONIC ACID INDUCED NEUROTOXICITY IN RATS
Abstract
This research was performed to characterize the neuroprotective effect of ethanolic extract of Dalbergia sissoo leaves in 3-Nitropropionic acid induced neurotoxic rats. The ethanolic extract of Dalbergia sissoo leaves was administered orally at different doses (300 and 600 mg/kg) to neurotoxic rats. During treatment psychopharmacological parameters were recorded, 24 hours after experiment antioxidant profiles from brain isolate were estimated and histopathology of brain was performed. The ethanolic extract significantly attenuated behavioral alterations, oxidative damage, mitochondrial dysfunction, and striatal/hippocampus damage in 3-Nitropropionic acid treated rats. These results suggest that ethanolic extract of Dalbergia sissoo leaves may have potential therapeutic value in the treatment of some neurological disorders, probably by its anti-inflammatory, antioxidant and estrogenic properties.
Keywords:
3-Nitropropionic acid, Huntington’s disease, Excitotoxicity, Oxidative stress, Dalbergia sissooDOI
https://doi.org/10.25004/IJPSDR.2014.060107References
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8. Fontaine MA, Geddes JW, Banks A, Butterfield DA. Effect of exogenous and endogenous antioxidants on 3-nitropionic acid-induced in vivo oxidative stress and striatal lesions: insights into Huntington’s disease. J Neurochem. 2000; 75: 1709-1715.
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13. Pankaj SN, Dharmendra S, Kiran P, K J. Antidiabetic activity of ethanolic extract of Dalbergia sissoo L. Leaves in alloxan-induced diabetic rats. ijcpr. 2010; 2(2): 24-27.
14. Adedotun AA, Alexander BO. Laboratory assessment of molluscicidal activity of crude aqueous and ethanolic extracts of Dalbergia sissoo plant parts against Biomphalaria pfeifferi. TMAID. 2008; 6: 219–227.
15. Josephin NR, Venkatarathnakumar T, Gowra R, Raadhika K et.al. Pharmacognostic and preliminary phytochemical evaluation of the leaves of Dalbergia sissoo Roxb. Asian J Pharm Clin Res. 2012; 5(3): 115-119.
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17. Puneet K, Kumar A. Effects of root extract of Withania somnifera in 3- Nitropropionic Acid-Induced Cognitive Dysfunction and Oxidative Damage in Rats. Int J Health Res. 2008; 1(3): 139-149.
18. Manuj A, Mahendra B, Chopra K. Protective effect of minocycline, a semi synthetic second-generation tetracycline against 3-nitropropionic acid(3-NP)-induced neurotoxicity. Toxicology. 2008; 244: 111-122.
19. Chao G, Li T, Miaomaio X, Haifan Y, Hailong D. Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats. J Ethnopharmacol. 2012; 144: 768-774.
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21. Puneet K, Kumar A. Protective role of sertraline against 3-nitropropionic acid-induced cognitive dysfunction and redox ratio in striatum, cortex and hippocampus of rat brain. Indian J Exp Biol. 2009; 47: 715-722.
22. Moshahid KM, Ajmal A, Tauheed I, Badruzzaman K, Nasrul H M, et.al. Resveratrol attenuates 6-hydroxydopamine-induced oxidative damage and dopamine depletion in rat model of Parkinson's disease. Brain Res. 2010; 1328: 139-151.
23. Nicolas A, Gillet JP, Fialip J, Coudore F. An animal model of nociceptive peripheral neuropathy following repeated cisplatin injections. Expl Neurol. 2003; 182: 12-20.
24. Sandeep K, Usha KM, Jayantee K, Vinay KK, Yahiya KM. Imbalance in oxidant/antioxidant system in different brain regions of rat after the infection of Japanese encephalitis virus. Neurochem Int. 2009; 55: 648-654.
25. Anil kumar MD, Inamdar NM. Evaluation of beneficial effects of antioxidant properties of aqueous leaf extract of andrographis paniculata in STZ-induced diabetes. Pak J Pharm Sci. 2009; 22: 49-52.
26. Hiroshi O, Nobuko O, Yagi K. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Anal Biochem. 1979; 95: 35l-358.
27. Ellman GL. Tissue Sulphydral Groups. Arch Biochem Biophys. 1959; 82: 70-77.
28. Deepak KD, Veerendra CY, Siva SN, Tirtha G, Rajalingam D, et.al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res. 2007; 6(3): 755-765.
29. Anusha J, Flora SJ. Dose related effects of nicotine on oxidative injury in young, adult and old rats. J. Environ. Biol. 2012; 33: 233-238.
30. Young DS, Pestaner LC, Gibberman V. Effects of drugs on clinical laboratory tests. Clin. Chem. 1975; 21: 351-353.
31. Friedman RB. Anderson RE, Hirshberg SB. Effects of diseases in clinical laboratory tests. Clin.Chem. 1980; 26: 209-211.
32. Chandrashekhar VM, Ranipariya VL, Ganapaty S, Parashar, Muchandi AA. Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats. J Ethanopharmacol. 2010; 127: 645-651.
33. Tariq M, Khan HA, Elfaki I, Deeb SA, Moutaery KA. Neuroprotective effect of nicotine againt 3-nitropropionic acid induced experimental Huntingtons disease in rats. Brain Res Bull. 2005; 67: 161-168.
34. Tunez I, Inmaculada T, Perez-De La Cruz V, Santamaria A. 3-Nitropropionic Acid as a Tool to Study the Mechanisms Involved in Huntington’s Disease: Past, Present and Future. Molecules. 2010; 15: 878-916.
35. Kumar P, Kumar A. Neuroprotective effect of cyclosporine and FK506 againt 3 nitropropionic acid induced cognitive dysfunction and glutathione redox in rat: possible role of nitric oxide. Neurosci Res. 2009; 63: 302-314.
36. Hajare SW, Chandra S, Sharma J, Tandan SK, Lal J et al. Anti-inflammatory activity of Dalbergia sissoo leaves. Fitoterpia. 2001; 72: 131-139.
37. Kamal H, Shaltout, Keshta AE. The biology of Egyptian woody perennials. Ass.Univ.Environ.Res. 2011; 14(2): 132-153.
38. Hajare SW, Chandra S, Tandan SK et al. Analgesic and Antipyretic activities of Dalbergia sissoo leaves. Indian J Pharmacol. 2000; 32: 357-360.
2. Beal MF, Brouillet E, Jenkins BG, Ferrante RJ, Kowall NW, Miller JM. Neurochemical and histological characterization of striatal excitotoxic lesions produced by the mitochondrial toxin 3-nitropropionic acid. J Neurosci. 1993; 13: 4181-4192.
3. Ludolph AC, He F, Spencer PS, Hammerstad, J, Sabri, M. 3-Nitropropionic acid–exogenous animal neurotoxin and possible human striatal toxin. Can. J Neurol Sci. 1991; 18: 492-498.
4. Coles CJ, Edmondson DE, Singer TP. Inactivation of succinate dehydrogenase by 3-nitropropionate. J Biol Chem. 1979; 254: 5161-5167.
5. Brouillet E, Guyot MC, Mittoux V, Altairac, S, Conde F, Palfi S. Partial inhibition of brain succinate dehydrogenase by 3-nitropropionic acid is sufficient to initiate striatal degeneration in rat. J Neurochem. 1998; 70: 794-805.
6. Schulz JB, Henshaw DR, MacGarvey U, Beal MF. Involvement of oxidative stress in 3-nitropropionic acid neurotoxicity. Neurochem Int. 1996; 29:167-171.
7. Beal MF. Does impairment of energy metabolism result in excitotoxic neuronal death in neurodegenerative illnesses? Ann Neurol. 1992; 31: 119-130.
8. Fontaine MA, Geddes JW, Banks A, Butterfield DA. Effect of exogenous and endogenous antioxidants on 3-nitropionic acid-induced in vivo oxidative stress and striatal lesions: insights into Huntington’s disease. J Neurochem. 2000; 75: 1709-1715.
9. Guyot MC, Hantraye P, Dolan R, Palfi S, Maziere M, Brouillet E. Quantifiable bradykinesia, gait abnormalities, and Huntington’s disease-like striatal lesions in rats chronically treated with 3-nitropropionic acid. Neuroscience. 1997; 79: 45-56.
10. Matthias L, Reichmann H. Clinical neuroprotection in Parkinson's disease- still waiting for the breakthrough. J Neurol Sci. 2010; 289: 104-114.
11. Madhava chetty K, Sivaji K, Tulasi rao K. Flowering plants of chittoor district Andhra Pradesh, India. Edn 1, Students offset printers, Tirupati, 2008, pp 88.
12. Mukhtar HS, Irum M, Khan SN. Medicinal importance and association of pathological constraints with Dalbergia sissoo. Pak J Phytopathol. 2010; 22(2): 135-138.
13. Pankaj SN, Dharmendra S, Kiran P, K J. Antidiabetic activity of ethanolic extract of Dalbergia sissoo L. Leaves in alloxan-induced diabetic rats. ijcpr. 2010; 2(2): 24-27.
14. Adedotun AA, Alexander BO. Laboratory assessment of molluscicidal activity of crude aqueous and ethanolic extracts of Dalbergia sissoo plant parts against Biomphalaria pfeifferi. TMAID. 2008; 6: 219–227.
15. Josephin NR, Venkatarathnakumar T, Gowra R, Raadhika K et.al. Pharmacognostic and preliminary phytochemical evaluation of the leaves of Dalbergia sissoo Roxb. Asian J Pharm Clin Res. 2012; 5(3): 115-119.
16. Ghosh MN. Fundamental of experimental pharmacology. 3rd ed. Mumbai: Hilton and Company; 2005.
17. Puneet K, Kumar A. Effects of root extract of Withania somnifera in 3- Nitropropionic Acid-Induced Cognitive Dysfunction and Oxidative Damage in Rats. Int J Health Res. 2008; 1(3): 139-149.
18. Manuj A, Mahendra B, Chopra K. Protective effect of minocycline, a semi synthetic second-generation tetracycline against 3-nitropropionic acid(3-NP)-induced neurotoxicity. Toxicology. 2008; 244: 111-122.
19. Chao G, Li T, Miaomaio X, Haifan Y, Hailong D. Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats. J Ethnopharmacol. 2012; 144: 768-774.
20. Shalavandl MH, Chandrasekhar VM, Avinash SP, Sowmya c, et.al. Neuroprotective activity of stereospermum suaveolens DC against 6-OHD induced Parkinson’s model. Indian J Pharmacol. 2012; 44(6): 737-743.
21. Puneet K, Kumar A. Protective role of sertraline against 3-nitropropionic acid-induced cognitive dysfunction and redox ratio in striatum, cortex and hippocampus of rat brain. Indian J Exp Biol. 2009; 47: 715-722.
22. Moshahid KM, Ajmal A, Tauheed I, Badruzzaman K, Nasrul H M, et.al. Resveratrol attenuates 6-hydroxydopamine-induced oxidative damage and dopamine depletion in rat model of Parkinson's disease. Brain Res. 2010; 1328: 139-151.
23. Nicolas A, Gillet JP, Fialip J, Coudore F. An animal model of nociceptive peripheral neuropathy following repeated cisplatin injections. Expl Neurol. 2003; 182: 12-20.
24. Sandeep K, Usha KM, Jayantee K, Vinay KK, Yahiya KM. Imbalance in oxidant/antioxidant system in different brain regions of rat after the infection of Japanese encephalitis virus. Neurochem Int. 2009; 55: 648-654.
25. Anil kumar MD, Inamdar NM. Evaluation of beneficial effects of antioxidant properties of aqueous leaf extract of andrographis paniculata in STZ-induced diabetes. Pak J Pharm Sci. 2009; 22: 49-52.
26. Hiroshi O, Nobuko O, Yagi K. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Anal Biochem. 1979; 95: 35l-358.
27. Ellman GL. Tissue Sulphydral Groups. Arch Biochem Biophys. 1959; 82: 70-77.
28. Deepak KD, Veerendra CY, Siva SN, Tirtha G, Rajalingam D, et.al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res. 2007; 6(3): 755-765.
29. Anusha J, Flora SJ. Dose related effects of nicotine on oxidative injury in young, adult and old rats. J. Environ. Biol. 2012; 33: 233-238.
30. Young DS, Pestaner LC, Gibberman V. Effects of drugs on clinical laboratory tests. Clin. Chem. 1975; 21: 351-353.
31. Friedman RB. Anderson RE, Hirshberg SB. Effects of diseases in clinical laboratory tests. Clin.Chem. 1980; 26: 209-211.
32. Chandrashekhar VM, Ranipariya VL, Ganapaty S, Parashar, Muchandi AA. Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats. J Ethanopharmacol. 2010; 127: 645-651.
33. Tariq M, Khan HA, Elfaki I, Deeb SA, Moutaery KA. Neuroprotective effect of nicotine againt 3-nitropropionic acid induced experimental Huntingtons disease in rats. Brain Res Bull. 2005; 67: 161-168.
34. Tunez I, Inmaculada T, Perez-De La Cruz V, Santamaria A. 3-Nitropropionic Acid as a Tool to Study the Mechanisms Involved in Huntington’s Disease: Past, Present and Future. Molecules. 2010; 15: 878-916.
35. Kumar P, Kumar A. Neuroprotective effect of cyclosporine and FK506 againt 3 nitropropionic acid induced cognitive dysfunction and glutathione redox in rat: possible role of nitric oxide. Neurosci Res. 2009; 63: 302-314.
36. Hajare SW, Chandra S, Sharma J, Tandan SK, Lal J et al. Anti-inflammatory activity of Dalbergia sissoo leaves. Fitoterpia. 2001; 72: 131-139.
37. Kamal H, Shaltout, Keshta AE. The biology of Egyptian woody perennials. Ass.Univ.Environ.Res. 2011; 14(2): 132-153.
38. Hajare SW, Chandra S, Tandan SK et al. Analgesic and Antipyretic activities of Dalbergia sissoo leaves. Indian J Pharmacol. 2000; 32: 357-360.
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01-01-2014
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“NEUROPROTECTIVE EVALUATION OF LEAF EXTRACT OF DALBERGIA SISSOO IN 3-NITROPROPIONIC ACID INDUCED NEUROTOXICITY IN RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 1, Jan. 2014, pp. 41-47, https://doi.org/10.25004/IJPSDR.2014.060107.
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How to Cite
“NEUROPROTECTIVE EVALUATION OF LEAF EXTRACT OF DALBERGIA SISSOO IN 3-NITROPROPIONIC ACID INDUCED NEUROTOXICITY IN RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 1, Jan. 2014, pp. 41-47, https://doi.org/10.25004/IJPSDR.2014.060107.
