RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PERINDOPRIL AND LOSARTAN IN PURE FORM AND TABLET FORMULATIONS

Authors

  • R. Vijayalakshmi GIET School of Pharmacy, Rajahmundry-533296, Andhra Pradesh, India
  • P. Sandya KGRL College of pharmacy, Bhimavaram, West Godavari, Andhra Pradesh, India
  • M. Poorna Chandra Rao Mahindra Satyam, Hyderabad, Andhra Pradesh, India
  • M. D. Dhanaraju GIET School of Pharmacy, Rajahmundry-533296, Andhra Pradesh, India

Abstract

The combination of Perindopril (PRN) and Losartan (LRN) is used in the management of hypertension. The present work was focused on the development of RP-HPLC method for the estimation of perindopril and losartan in binary mixtures. The separation was performed on LUNA C18 column at 210 nm by isocratic elution. Methanol and phosphate buffer (pH 6.8) in the ratio of 85:15 with a flow rate of 0.8 ml/min at ambient temperature was found to be suitable for chromatographic separation. Perindopril and losartan exhibits linearity in the ranges of 200-500 and 30-80 µg/mL respectively. The proposed method evidenced the absence of chromatographic interference by pharmaceutical excipients. Separation of the mixtures was proved to be good from the resolution of the peaks. Validation and recovery studies were performed and the results proved the method to be suitable for routine analysis.

Keywords:

Perindopril, Losartan, RP-HPLC, Simultaneous estimation

DOI

https://doi.org/10.25004/IJPSDR.2014.060113

References

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Published

01-01-2014
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How to Cite

“RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PERINDOPRIL AND LOSARTAN IN PURE FORM AND TABLET FORMULATIONS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 1, Jan. 2014, pp. 75-77, https://doi.org/10.25004/IJPSDR.2014.060113.

Issue

Volume 6, Issue 1, 2014

Section

Research Article

Copyright (c) 2014 R. Vijayalakshmi, P. Sandya, M. Poorna Chandra Rao, M. D. Dhanaraju

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

“RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PERINDOPRIL AND LOSARTAN IN PURE FORM AND TABLET FORMULATIONS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 1, Jan. 2014, pp. 75-77, https://doi.org/10.25004/IJPSDR.2014.060113.