ANTI-INFLAMMATORY ACTIVITY AND TOTAL FLAVONOID CONTENT OF AEGLE MARMELOS SEEDS
Abstract
The anti-inflammatory effect of aqueous and methanol extracts of Aegle marmelos seeds was evaluated using carrageenan induced paw edema and cotton pellets induced granuloma in rats. To prove the dependency of pharmacological activity on certain phytoconstituent, total flavonoid contents were estimated, using a spectrophotometric technique. The Quercetin was used as standard compound and the total flavonoids were expressed as mg / g quercetin equivalents (Standard curve equation: y= 0.0009X + 0.0011, R2 = 0.9189). The total flavonoid varied from 3.267 to 12.933 mg/g in extracts. The maximum flavonoid content was found in the methanolic extract (65.20 ± 0.2 mg/g). The methanolic extract administered orally at doses of 200 and 400 mg/kg, b.w. showed highly significant (P<0.01) anti-inflammatory activity. The aqueous extract also produced significant (P<0.05) anti-inflammatory property at the same doses.
Keywords:
Aegle marmelos, Flavonoid, Quercetin, Inflammation, Carrageenan.DOI
https://doi.org/10.25004/IJPSDR.2011.030310References
1. Kumar V, Abbas AK, Fausto N. Robbins and Cotran: Pathologic basis of disease, 7th edition, Elsevier Saunders, Philadelphia, Pennsylvania, 2004, pp. 47-86.
2. Sosa S, Balicet MJ, Arvigo R, Esposito RG, Pizza C, Altinier G. Screening of the topical anti-inflammatory activity of some Central American plants. J. Ethanopharmacol. 2002; 8: 211-215.
3. Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 2000; 32:81-118.
4. Harborne JB. Phytochemical methods, Second edition, By Chapman and Hall, London and New York 1984; 37(3):19-25.
5. Marucci MC, Ferreres F, Garcia-Viguera C, Bankova VS, De Castro SL, Dantas AP, Valente PHM, Paulino N. Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol. 2001; 74:105-112.
6. Tapiero H, Tew KD, Nguyen BAG, Mathe G. Polyphenols: do they play a role in the prevention of human pathologies?” Biomed. Pharmacother. 2002; 56: 200-207.
7. Tunon MJ, Garcia MMV, Sanchez CS, Gonzalez GJ. Potential of flavonoids as anti-inflammatory agents: modulation of pro-inflammatory gene expression and signal transduction pathways. Curr Drug Metab. 2009; 10(3):256-271.
8. Ana GL, Eva G, Ana V, Mauricio AR, Jose AM. Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflammation Research 2009; 58(9):537-552.
9. Kim H.P, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004; 96: 229-245.
10. Robert JN, Elsvan N, Danny EH, Petra GB, Klaske N, Paul AM. Flavonoids: a review of probable mechanisms of action and potential applications. Ameri. J. Clin. Nutri. 2001; 74(4): 418-425.
11. Sathishkumar T, Baskar R, Shanmugam S, Rajasekaran P, Sadasivam S, Manikandan V. Optimization of flavonoids extraction from the leaves of Tabernaemontana heyneana Wall. using L16 Orthogonal design. Nature and Science 2008; 6(3):10-21.
12. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64:555-559.
13. Schultz SC, Bahraminejad S, Asenstorfer RE, Riley IT. Analysis of the antimicrobial activity of flavonoids and saponins isolated from the sshoots of oats (Avena sativa L.). J. Phyto Path. 2008; 156:1–7.
14. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Analysis 2002; 10:178-182.
15. Kosalec I. Flavonoid analysis and antimicrobial activity of commercially available propolis products. Acta. Pharm. 2005; 55:423-430.
16. C.S.I.R., “The wealth of India” National Institute of Science communication and Information Resources”, 1985, I (A), pp. 86.
17. Sharma PC, Bhatia V, Bansal N, Sharma A. A review on bael tree. Natural Produts Radiance 2007; 6(2):171-178.
18. Maity P, Hansda D, Bandyopadhyay U, Mishra DK. Biological activities of crude extracts of chemical constituents of Bael, Aegle marmelos (L.) Corr. Indian Journal of Experimental Biology 2009; 47: 849-861.
19. Winter C, Risley E, Nuss G. Carrageenan induced oedema in hind paw of rats- an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med. 1962; 11: 54.
20. D’Arcy P, Howard E, Muggletone P, Townsend S. The anti-inflammatory action of Griseofulvin in experimental animals. J. Pharm. Pharmacol. 1960; 12: 659.
2. Sosa S, Balicet MJ, Arvigo R, Esposito RG, Pizza C, Altinier G. Screening of the topical anti-inflammatory activity of some Central American plants. J. Ethanopharmacol. 2002; 8: 211-215.
3. Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian Journal of Pharmacology 2000; 32:81-118.
4. Harborne JB. Phytochemical methods, Second edition, By Chapman and Hall, London and New York 1984; 37(3):19-25.
5. Marucci MC, Ferreres F, Garcia-Viguera C, Bankova VS, De Castro SL, Dantas AP, Valente PHM, Paulino N. Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol. 2001; 74:105-112.
6. Tapiero H, Tew KD, Nguyen BAG, Mathe G. Polyphenols: do they play a role in the prevention of human pathologies?” Biomed. Pharmacother. 2002; 56: 200-207.
7. Tunon MJ, Garcia MMV, Sanchez CS, Gonzalez GJ. Potential of flavonoids as anti-inflammatory agents: modulation of pro-inflammatory gene expression and signal transduction pathways. Curr Drug Metab. 2009; 10(3):256-271.
8. Ana GL, Eva G, Ana V, Mauricio AR, Jose AM. Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflammation Research 2009; 58(9):537-552.
9. Kim H.P, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004; 96: 229-245.
10. Robert JN, Elsvan N, Danny EH, Petra GB, Klaske N, Paul AM. Flavonoids: a review of probable mechanisms of action and potential applications. Ameri. J. Clin. Nutri. 2001; 74(4): 418-425.
11. Sathishkumar T, Baskar R, Shanmugam S, Rajasekaran P, Sadasivam S, Manikandan V. Optimization of flavonoids extraction from the leaves of Tabernaemontana heyneana Wall. using L16 Orthogonal design. Nature and Science 2008; 6(3):10-21.
12. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64:555-559.
13. Schultz SC, Bahraminejad S, Asenstorfer RE, Riley IT. Analysis of the antimicrobial activity of flavonoids and saponins isolated from the sshoots of oats (Avena sativa L.). J. Phyto Path. 2008; 156:1–7.
14. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Analysis 2002; 10:178-182.
15. Kosalec I. Flavonoid analysis and antimicrobial activity of commercially available propolis products. Acta. Pharm. 2005; 55:423-430.
16. C.S.I.R., “The wealth of India” National Institute of Science communication and Information Resources”, 1985, I (A), pp. 86.
17. Sharma PC, Bhatia V, Bansal N, Sharma A. A review on bael tree. Natural Produts Radiance 2007; 6(2):171-178.
18. Maity P, Hansda D, Bandyopadhyay U, Mishra DK. Biological activities of crude extracts of chemical constituents of Bael, Aegle marmelos (L.) Corr. Indian Journal of Experimental Biology 2009; 47: 849-861.
19. Winter C, Risley E, Nuss G. Carrageenan induced oedema in hind paw of rats- an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med. 1962; 11: 54.
20. D’Arcy P, Howard E, Muggletone P, Townsend S. The anti-inflammatory action of Griseofulvin in experimental animals. J. Pharm. Pharmacol. 1960; 12: 659.
Published
01-07-2018
Statistics
Abstract Display: 339 
PDF Downloads: 427
Dimension Badge
How to Cite
“ANTI-INFLAMMATORY ACTIVITY AND TOTAL FLAVONOID CONTENT OF AEGLE MARMELOS SEEDS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 3, July 2018, pp. 214-8, https://doi.org/10.25004/IJPSDR.2011.030310.
Issue
Section
Research Article
How to Cite
“ANTI-INFLAMMATORY ACTIVITY AND TOTAL FLAVONOID CONTENT OF AEGLE MARMELOS SEEDS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 3, July 2018, pp. 214-8, https://doi.org/10.25004/IJPSDR.2011.030310.
