ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACTS OF LEAF AND STEM BARK OF CALOPHYLLUM INOPHYLLUM ON ALBINO WISTAR RATS
Abstract
The aim of the present study was to conduct phytochemical screening, perform acute oral toxicity effect and to evaluate the anti-inflammatory effect of ethanolic extracts of leaf and stem bark of Calophyllum inophyllum on albino Wistar rats. Carrageenan induced paw edema and cotton pellet granuloma techniques were applied to determine anti-inflammatory properties of the extracts. Extracts were administered orally. Acute oral toxicity studies were conducted using the OECD guidelines 423 Annexure – 2d. The results indicate the mortality was not observed during the toxicity studies and maximum safe does was determined. The anti-inflammatory effect of the extracts showed significant dose dependent effect (200 mg/kg b.w and 400 mg/kg b.w) on both acute and chronic models of inflammation i.e., carrageenan induced paw edema and cotton pellet granuloma respectively. Additionally, Calophyllum inophyllum leaves extract showed more activity compared to Calophyllum inophyllum stem bark extract.
Keywords:
Calophyllum inophyllum, Calophyllum inophyllum leave extract, Calophyllum inophyllum stem bark extract, anti-inflammatory effect, carrageenan induced paw edema, cotton pellet induced granulomaDOI
https://doi.org/10.25004/IJPSDR.2014.060218References
2. Dweck AC, Meadowsy T. Tamanu (Calophyllum inophyllum) – the African, Asian, Polynesian and Pacific Panacea. Int J Cosmetic Science 2002; 24: 1-8.
3. Yoon J, Baek SJ. Molecular targets of dietary polyphenols with anti-inflammatory properties. Yonsei Med J. 2005; 46:585-596.
4. Quan L, Thiele GM Tian J, Wang D. The development of novel therapies for Rheumatoid Arthritis. Expert Opin. Ther. Pat.2008; 18:723-738.
5. OECD Test Guidelines 423, acute oral toxicity - Acute Oral Class methods, 2001.
6. Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. 1962; 111: 544-547.
7. Hicks R. The evaluation of inflammation induced by material implanted subcutaneously in the rat. J Pharm Pharmacol. 1969; 21:581-588.
8. Dannhardt G, Kiefer W. Cyclooxygenase inhibitors- current status and future prospects. Eur J Med Chem. 2001; 36:109-126.
9. Mequanint W, Makonnen E, Urga K. In-vivo anti-inflammatory activities of leaf extracts of Ocimum lamiifolium in mice model. J Ethnopharmacol. 2011; 134:32-36.
10. Suralkar AA, Rodge KN, Kamble RD, Maske KS. Evaluation of anti-inflammatory and analgesic activities of Tamarindus indica. International Journal of Pharmaceutical Sciences and Drug Research 2012; 4(3): 213-217.
11. Küpeli E, Yesilada E. Flavonoids with anti-inflammatory and antinociceptive activity from Cistus laurifolius L. leaves through bioassay-guided procedures. J Ethnopharmacol. 2007; 112:524-30
12. Clavin M, Gorzalczany S, Macho A, Muñoz E, Ferraro G, Acevedo C, Martino V. Anti-inflammatory activity of flavonoids from Eupatorium arnottianum. J Ethnopharmacol. 2007; 112:585-589
13. Lavaud A, Soleti R, Hay AE, Richomme P, Guilet D, Andriantsitohaina R. Paradoxical effects of polyphenolic compounds from Clusiaceae on angiogenesis. PMID: 22177987.
Published


How to Cite
Issue
Section
Copyright (c) 2014 Mirza Danish Baig, Syed Basheeruddin, Silpa S., A. Venkateshwara Reddy

This work is licensed under a Creative Commons Attribution 4.0 International License.