Potential investigation of Antioxidant and Anti-inflammatory activities in In vitro Root tubers of Holostemma annulare (Roxb.)K Schum
Abstract
Holostemma annulare is a twining perennial shrub belonging to the family Asclepiadaceae with therapeutic and pharmacological potentialities. The root tuber of the plant is rich in antioxidants and have been suggested as potential sources of anti-inflammatory compounds. Thus the antioxidant and anti- inflammatory activities of the methanolic in vivo and in vitro root tuber extracts of the H. annulare with wide spread use in folk medicine were evaluated in this study. Antioxidant activity was evaluated by DPPH, Nitric oxide, Hydrogen peroxide, Superoxide scavenging and Reducing power activity assays. In addition, in vitro anti-inflammatory activity of the methanol root tuber extracts was evaluated by proteinase inhibition assay, COX and LOX assays. The results revealed that in vitro root tuber extracts possesses significant antioxidant and anti- inflammatory activities to the extent of in vivo tubers and thus can be explored as an important sources of novel antioxidant and anti- inflammatory agents. This application also emphasizes the potential utilization of in vitro tubers of the species for drug formulations.
Keywords:
Holostemma annulare, in vitro root tubers, antioxidants, anti-inflammatory activityDOI
https://doi.org/10.25004/IJPSDR.2022.140501References
Samuelson B, Dahlen SE, Lindgren JA, Rouzer CA, Serhan CN. Leukotrienes and lipoxins: tructures, biosynthesis and biological effects. Science. 1987; 237:1171-1176.
Ved DK, Goraya GS. Demand and supply of medicinal plants in India. NMPB, New Delhi & FRLHT, Banglore, India, 2007.
Halliwall B, GutteridgeJM, Grootveld M, Methods for the measurements of hydroxyl radicals in biomedical systems; Deoxyribose degradation and aromatic hydroxylation. Methods of Biochemical Analysis. 1988; 33:59-90.
Viljoen A, Mncwangi N, Vermaak I. Anti-inflammatory iridoids of botanical origin. Current Medical Chemistry. 2012; 19(14):2104–27.
Akula US, Odhav B. In vitro 5-Lipoxygenase inhibition of polyphenolic antioxidants from undomesticated plants of South Africa. Journal of Medicinal plant Research. 2008; 2(9):207-212.
Malik J, Landa P, Kutil Z, Marsiks P, Kokoska L. In vitro COX-1, COX -2 and 5-LOX inhibitory activity of plant family anunculaceae. Planta Medica. 2011; 77(12):1059-1059.
Warrier PK, Nambiar VPK, Raman KC. Indian medicinal plants, a compendium of 500 species. Orient Longman, 1995, pp. 3: 165.
Harbone JB. Methods of plant analysis: In phytochemical methods, Champan and Hall. London, 1973, 4-16.
Sofowora A. Research on Medicinal plants and Traditional Medicine in Africa. Journal of Alternative and Complementary Medicine.1996; 2 (3): 365-372.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin -phenol reagent. J Biol Chem. 1951; 193:265- 275.
Hedge JE, Hofreiter BT. Methods in carbohydrate chemistry, Whistler RL and Bemiller JN, Academic Press, New York, 1962, 420.
Miller GL. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugars, Analytical chemistry, 1972, 31, 426-428.
Okwu DE, Okwu ME. Chemical composition of Spondias mombin Linn Plant parts. J Sustain Agric Environ. 2005; 6(2): 140- 147.
Singelton VL, Joseph Rossi A. Colorimetry of Tropical Phenolics with phosphomolybdic- phosphotungstic acid reagents. American Journal of enology & Vitriculture. 1965; 144-158.
Zhishen Y, Mengcheng T, Jianming W. Determination of flavanoid content in mulberry and their scavenging effect of superoxide radicals. Food chemistry. 1999; 64 (4):555- 559.
Indumathi C, Durgadevi G, Nithyavani S, Gayathri PK. Estimation of Terpenoid content and its antimicrobial property in Enicostemma litorrale. Int J Chem Tech Res 2014; (6):4264-4267.
Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heart wood. Journal of Agricultural and Food Chemistry. 2001; (49): 3420-3424.
Garret DC. The quantitative analysis of drugs, Champman and Hall, Japan, 1964; 3:456-458.
Elizabeth K, Rao MWA. Oxygen radical scavenging activity of Curcumin. International journal of pharmaceutics. 1990; 58: 237-240.
Valentao P, Fernandes E, Carvalho F, Andrade P. Studies on the antioxidant activity of Lippia citriodora infusion: Scavenging effect on Superoxide Radical, Hydroyl radical and Hypochlorous acid. Biological and Pharmaceutical Bulletin. 2002; 25(10):1324-1327.
Yen GC, Duh PD. Antioxidative properties of methanolic extracts from Peanut hulls. Journal of the American oil chemists society. 1993; 70(4):383-386.
Sakat SS, Juvekar AR, Gambhire M. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharmacy and Pharmaceutical Sciences. 2010; 2(1):146–155.
Walker MC, Gierse JK. In Vitro assays for cyclooxygenase Activity and Inhibitor Characterization. Methods in Molecular Biology. 2010; 644: 131-44.
Axelrod B, Cheesebrough TM, Laakso S. Lipoxygenase from soyabean. Methods in Enzymology. 1981; 71:441-451.
Sakthidevi G, Mohan VR. Total Phenolic, Flavonoid Contents and in vitro antioxidant activity of Dioscorea alata l. Tuber. Journal of Pharmaceutical science and Research. 2013; 5(5): 115 – 119.
Afsar T, Trembley JH, Salomon CE, Razak S, Khan MR, Ahmed K. Growth inhibition and apoptosis in cancer cells induced by polyphenolic compounds of Acacia hydaspica: Involvement of multiple signal transduction pathways. Scientific Reports. 2016; 6:2-12
Del-Rio A, Obdulio BG, Castillo J, Marin FR, Ortuno A. Uses and properties of Citrus flavonoids. Journal of Agriculture and Food Chemistry. 1997; 45(12):4505-4515.
Veerachari U, Bopaiah AK. Preliminary phytochemical evaluation of the leaf extract of five Cassia species. Journal of Chemical and Pharmaceutical Research. 2011; 3(5):574- 583.
Awoyinka AO, Balogun IO, Ogunnowo AA. Phytochemical screening and in vitro bioactivity of Cnidoscolus aconitiifolus (Euphorbiaceae). Journal of Medicinal Plant Research. 2007; 1(3): 63-65.
Monika J, Anil B, Aakanksha B, Priyanka P. Isolation, Characterization and In vitro Anti-urolithiatic activity of Cerpegin Alkaloid from Ceropegia bulbosa var. lushii root. International Journal of Drug Development and Research. 2012; 4(4): 154- 160.
Duke J. Handbook of Biological Active Phytochemical and its derivatives. Boca Raton (FL) CRC Press, 1992, pp. 99-131.
Jesika R, Rajesh j, Bakel RL. Liver diseases and herbal drugs: A review, Journal of innovations in pharmaceutical and biological sciences.2016; 3(2): (2016) 24-36.
Paduch R, Szerszen MK, Trytek M, Fiedurek J. Terpenes: substances useful in human healthcare. Archivum Immunologiae et Therapiae Experimentalis, 2007; 55(5): 315-327.
Poyton RO, Ball KA, Castello PR. Mitochondrial generation of free radicals and hypoxic signaling. Trends in Endocrinology and Metabolism. 2009; 20(7):332- 40.
Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How are they linked? Free Radical Biology and Medicine. 2010; 49(11):1603-16.
Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanol extract of five phyllanthus species from India. LWT Food science and Technology. 2007; 40 (2): 344-352.
Miller M J, Sadowska-krowicka H, Chotinaruemol S, Kakkis JL and Clark DA. Amelioration of chronic ileitis by nitric oxide synthase inhibition. Journal of Pharmacology and Experimental Theraputicals. 1993; 264: 11.
Murrell GA, Doland MM, Jang D, Szabo C, Warren RF, Hannafin JA. Nitric oxide: an important articular free radical. Journal of Bone and Joint Surgery. 1996; 78(2): 265-274.
Hoffseth JL, Hussain SP, Wogan G, Harris C. Nitricoxide in cancer and chemoprevention. Free Radical Biology and Medicine. 2003; 34(8):955-68.
Gulcin I, Berashvili D, Gepdiremen. Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne. Journal of Ethno pharmacology .2005; 101:287–293.
Sahreen S, Khan MR, Khan RA. Phenolic compounds and antioxidant activities of Rumex hastatus D. Don. Leaves. Jounal of Medicinal Plants Research. 2011; (5):2755–2765.
Naskar S, Islam A, Mozumdes UK, Saha P, Haldar PK., Gupta M. In vitro and in vivo antioxidant potential of hydromethanolic extract of Phoenix dactylijera fruits. Journal of Scientific Research. 2010; 2(1):144-157.
Wong CC, Li HB, Cheng KW, Chen F. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem. 2006; 97:705-11.
Dub PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng lyur (Chrysabthemum moifolium Ramat). Food science and Technology -lebensmittel Wissenschaft Technologie. 1999; 32(5): 269-277.
Gordon MF. “The mechanism of the antioxidant action in vitro”. In: B.J.F, Ed, Food antioxidants: London, Elsevier, 1990, pp: 1-18.
Jellyy Louis. A study on the production of antineoplastic secondary metabolites from selected medicinal plants using cell and tissue culture techniques (with special reference to Ophiorrhiza mungos and Holostemma ada-kodien), Ph.D Thesis, Mahathma Gandhi univ; Amala research centre, Thrissur, Kerala, 2010.
Mallikarjuna B, Usha NR, Gayathri D, Ramagopal G. In vitro antioxidant and free radical scavenging potential of Holostemma ada-kodien- An important rare medicinal plant. International journal of Pharmaceutical science and research. 2013; 2: 2413- 2418.
Rubesh Kumar S, Sridhar C, Jayaveera K N. A Study on Total Phenols, Flavonoids Content and Antioxidant activity for the leaf extracts of Holostemma ada-Kodien Schult traditionally used folklore medicinal plants. Indo American Journal of Pharmaceutical Research. 2014; 4(2).
Kaffoor AH, Muthuraj K, Arumugasamy K. Antioxidant activity of leaf extracts of Hemidesmus indicus (L.) R.BR. (Asclepedeaceae). Asian journel of Pharmaceutical and Clinical Reasearch. 2016; 10(6).
Risso WE, Scarminio IS, Moreira EG. Antinociceptive and acute toxicity evaluation of Vernonia condensata Baker leaves extracted with different solvents their mixtures. Indian Journal of Experimental Biology 2010; 48(8) :811-816.
Hesham R, El- Seedi, Shgeru N. Chemistry of bioflavonoids. Indian Journal of pharmaceutical education .2007; 39:172.
Di Carlo G, Mascolo N, Izzo AA, Capasso. Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999;65(4):337–53.
Rajnarayana K, Reddy MS, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian Journal of pharmacology. 2001; 33:2-16.
Yasodha Krishna J, Kumar Ponnusamy, Kahsay Getu , Anti-inflammatory activity of alcoholic extract of leaves of Holostemma ada-kodien Shult.in albino rats. International Journal of Pharmacy education and Research. 2016; 3(2) 13-16.
Daniel D, Subramaniyan S, Sandhia GS. In-Vitro Anti-inflammatory activity evaluation of the latex Protease of Holostemma ada-kodien Schult. International Journal of Pharma Research and Health Sciences. 2017; 5(4): 1794-99.
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