Assessment of Hepatoprotective Activity of Hyptis capitata Jacq. Against Oxidative Stress Induced by H2O2
Abstract
Medicinal plants occupy a key position in maintaining the health of the human population. Hyptis capitata Jacq. is an exotic ethnomedicinal plant of Lamiaceae possessing immense potential. The present study was carried out to assess the in vitro hepatoprotective activity of methanolic leaf extract of H. capitata against H2O2 induced oxidative stress in HepG2 cell lines. Total antioxidant capacity and Superoxide radical scavenging activity of the extract were also performed. The extract displayed considerable free radical scavenging activity (P < 0.01). MTT assay was used to assess the in vitro hepatoprotective activity in HepG2 cell lines. The extract displayed concentration-dependent cell viability with maximum protection (63.97 ± 1.23%) in H2O2 induced HepG2 cell lines at 100 μgml-1. H. capitata is a promising herb with significant antioxidant and hepatoprotective potential.
Keywords:
Key Words : Hyptis capitata Jacq. , Antioxidant, MTT assay, Hepatection, HepG2 cell lines.DOI
https://doi.org/10.25004/IJPSDR.2022.140106References
Ghasemzadeh A, Jaffar HZE, Rahmat A. Antioxidant activities , total phenols and flavonoids content in two varieties of Malaysia Young Ginger (Zingiber officinale Roscoe). Molecules. 2010 ; 15 : 4324-4333.
Zargari A. Medicinal Plants. Tehran University Press. 1992.
WHO Guidelines on Safety Monitoring of Herbal Medicines in Pharmacovigilance Systems. Geneva, Switzerland: 2004. World Health Organization
Nadeem MPC, Dandiya PC, Pasha M, Imran D, Balani K, Vohora, SB. Hepatoprotective activity of Solanum nigrum fruits. Fitoterpia. 1997; 68 (3) : 245-51.
Pratap SM, Tewari U, Sharma K, Jha K. Hepatoprotective activity of whole plant Leptadaenia pyrotechnica against paracetamol induced damage in rats. Journal of Drug Delivery. 2014; 4 : 36-39.
Pal S , B hattacharjee A , M ukherjee S , B hattacharya K , K howla S . Antioxidant and hepatoprotective activity of ethanolic extract of Alocasia indica tuber. American Journal of Phytomedicine and Clinical Therapeutics. 2014 ; 2: 191-208.
Constantin M, Bromont O, Fickat R, Massingham R. Studies on the activity of bepridil as a scavenger of free radicals .Biochemical Pharmacology. 1990; 40 : 1615-22.
Ajiboye TO, Yakubu MT, Oladiji AT, Akanji MA, Okogun JI. Antioxidant and drug detoxifiacation potential of aqueous extract of Annona senegalensis leaves in carbon tetra chloride induces hepatocellular damage. Pharmaceutical Biology. 2010 ; 48 : 1361-1370.
Harbone JB. Methods of Extraction and Isolation. In: Phytochemical Methods. Chapman and Hall, London, 1998, 60-66.
Prieto P, Pineda, M, Anguilar M. Spectrometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex : specific application to the determination of Vitamin E. Analytical Biochemistry. 1999;269;337-341.
Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced Phenazine methosulfate and molecular oxygen. Biochemical and Biophysical Research communication. 1972:46(2):849-854.
Sidduqui M.A, Ali Z, Chittiboyina AG. Khan IA. Hepatoprotective Effect of Steroidal Glycosides from Dioscorea villosa on Hydrogen peroxide induced toxicity in HepG2 cells. Front Pharmacol. 2018;9:797.
Cai Y, Luo Q, Sun M. Croke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science. 2004: (74) : 2157-2184.
Joseph JA, Shukitt-Hale B, Denisova NA, Bielinski D, Martin A, Mc Even JJ, Bideford, P.C. Journal of Neurosciene. 1999 ; 19(18): 8114-8121.
Pietta PG. Flavonoids as antioxidants. Journal of Natural Products. 2000 ; 63 :1035-1042.
Sumitha V, Mini I, Laija S Nair. Antioxidant activity of Hyptis capitata Jacq. –An in vitro study. International Journal of All Research Education and Scientific Methods. 2021 ; 9(6) : 1404- 1409.
Francis MA, Andrew WV. Antioxidant Activity, Nitrogen Oxide scavenging activity and Phenolic content of Ocimum gratissimum leaf extract. Journal of Medicinal Plant Research. 2010;4(24): 2479- 2487.
Abhilah G , M aheswari Y J, G opal A , C handa D . R eview o n s ome medicinal plant with hepato-protective activities. Research Review Journal of Pharmacognosy and Phytochemistry. 2014;4: 22- 33.
Lee, W.M. Drug-induced hepatotoxicity. New England Journal of Medicine. 2003 ; 349 : 474–485
Pari L, Satheesh MA. Antidiabetic activities of Boerhaavia diffusa L. effect on hepatic key enzymes in experimental diabetes. Journal of Ethnopharmacology. 2004 ; 9 (1) : 109-113.
Viswanath Jannu, Preetham Goud Baddam, Anil Kumar Boorgula. Satwik Reddy Jambble. “A review on Hepatoprotective plants’’. International Journal of Drug Development and Research. 2012; 4 (3) : 1-8.
Quanfang Huanga, Shijun Zhang , Renbin Huang , Ling Wei, Yongxing Chenb, Chunhong Liang and Shuanga Lianga.. Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpoides Lam. Journal of Ethnopharmacology. 2013; SO 378-8741 (13) 00642-49.
Gonzalo V, Susana M, Margarita G, Monika R, Tiziana F. Supercritical rosmary extracts, their antioxidant activity and effect on hepatic tumour progression. Journal of Supercritical Fluids. 2013;79: 101-108.
Rivera-Espinoza, Muriel, P. Pharmacologcal actions of curcumin in liver diseases or damage. Liver International. 2009; 29(10): 1457-1466.
Wan Xu-yinga, Luo Minga, Li Xiao-dongh, He Ping. Hepatoprotective and anti hepatocarcinogenic effect of glycirrhizin and matrine. Chemico- Biological Interactions. 2009 ; 181 : 15-19.
Chirdchuounseree H, Pramyothin P. Protective activity of Phyllanthin in ethanol treated primary culture of rat hepatocytes. Journal of Ethnopharmacology. 2010 ; 128 : 172-176.
Valan MF, John De Britto, Venkataraman R. Phytoconstituents with Hepatoprotective activity. International Journal of Chemical Sciences. 2010 ; 8(3) : 1421-1423.
Sumitha V, Mini.I, Laija S Nair.. Antimicrobial activity of Hyptis capitata Jacq. – An ethnomedicinal plant of Lamiaceae. International Journal of Botany Studies. 2021; 6(6) : 684-687.
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