IN VITRO CYTOTOXICITY ANALYSIS OF 5-FLUOROURACIL LOADED GUAR GUM MICROSPHERES ON HT-29 COLON CANCER CELL LINE
Abstract
The present investigation was aimed to develop a new formulation containing chemically cross-linked guar gum microspheres loaded with 5-fluorouracil for targeting of colorectal cancer. The emulsification polymerization method involving the dispersion of aqueous phase of guar gum in castor oil was used to prepare spherical microspheres. Cytotoxicity analysis on HT-29 human colon cancer cell lines indicated that 5-FU loaded guar gum microspheres leads to sustained releases of drug and thus delayed apoptosis over a long period of time. In this way, 5-fluorouracil loaded guar gum microspheres have shown promising results in the management of colorectal cancer, warranting thorough in vivo study for scale up technology.
Keywords:
Microspheres, 5-fluorouracil, HT-29 human colon cancer cell lines.DOI
https://doi.org/10.25004/IJPSDR.2009.010206References
Heidelberger, C, Chaudhuri, NK, Danneburg P. Fluorinated pyrimidine, A new class of tumor inhibitory compounds. Nature 1957; 179, 663-666.
Chourasia, MK, Jain SK. Pharmaceutical approaches to colon targeted drug delivery systems. J Pharm Pharm Sci 2003; 6: 33-66.
Wilding IR, Hardy JG, Sparrow RA, Davis SS, Duly PB, English JR. In vivo evaluation of enteric coated naproxan tablets using gamma scintigraphy. Pharm Res 1992; 1436-1441.
Khan MZ, Prebeg Z, Kurjakovic N. A pH dependent colon targeted oral drug delivery sytem using methacrylic acid copolymers I. Manipulation of drug releases using Eudragit L100-55and Eudragit S100 combinations. J Control Release 1999; 58: 215-222.
Lippold BH, Sutter BK, Lippold BC. Parameters controlling drug release from pellets coated with aqueous ethyl cellulose dispersions [J]. Int J Pharm 1989; 54: 15-25.
Choursia MK, Jain SK. Potential of guar gum microspheres for target specific drug release to colon [J]. J Drug Targeting 2004; 12 (7): 435-442.
Kaushik D, Sardana S, Mishra DN. 5-Fluorouracil loaded guar gum microspheres for colon delivery: Preparation, characterization, In vitro release and cytotoxicity evaluation. Acta Pharmaceutica Sinica [in press].
Published
Abstract Display: 241 
PDF Downloads: 385 
