ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC CRUDE EXTRACTS OF TURNERA ULMIFOLIA L.
Abstract
The present study was carried out to study the antimicrobial and antioxidant potential of ethanolic leaf extract of Turnera ulmifolia. The antimicrobial activity was tested against five bacteria. Among the five different bacteria used, in the case of S. typhi the zone of inhibition is higher (30.76 mm) in 40µg/ml concentration followed by 60µg/ml concentration (24.88 mm). In the case of E. coli the zone of inhibition of is higher in 20µg/ml concentration (31.2 mm) In P. aeruginosa also the maximum inhibition zone (20.70 mm) was observed in 20µg/ml concentration followed by 40µg/ml (17.28 mm). At 20µg/ml of concentration the zone of inhibition is higher (18.30 mm) in K. pneumonia .Where as in S. aureus the percentage of inhibition is 10.16 mm in 40µg/ml concentration. The extract was effective on all the five bacteria. The higher percentage of activity in DPPH was observed in 1000µg/ml (94.59%) followed by 800µg/ml (94.28%). More absorbance (0.174) was observed in the concentration of 1000µg/ml followed by 800µg/ml concentration (0.165) in FRAP activity. Antioxidant activity and reducing power of solvent extracts was found to be dose dependent manner.
Keywords:
Antimicrobial, Turnera ulmifolia, Concentration, DPPH, FRAP and InhibitionDOI
https://doi.org/10.25004/IJPSDR.2014.060410References
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28. Yang JH, Lin HC, Mau JL. Antioxidant properties of several commercial mushrooms. Food Chem. 2002; 77: 229-235.
2. Cook NC, Samman S. Flavonoids- Chemistry, metabolism, cardioprotective effects, and dietary sources. Journal of Nutritional Biochemistry 1996; 7: 66-76.
3. Manandhar NP. Plants and People of Nepal. Timber Press, USA. 2000, pp. 50.
4. Doughari JH, El-mahmood AM, Tyoyina I. Antimicrobial activity of leaf extracts of Senna obtusifolia (L). Afr J Pharm Pharmacol. 2008; 2:7–13.
5. Khan AV, Ahmed QU, Shukla I, Khan AA. Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pac J Trop Biomed. 2012; 2:189–194.
6. Arcanjo DDR, Albuquerque ACM, Melo-Neto B, Santana LCLR, Medeiros MGF, Citó AMGL. Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian North-eastern folk medicine. Braz J Biol. 2012; 72:505–509.
7. Duracková Z. Some Current Insights into Oxidative Stress. Physiological Research 2010; 59(4): 459-469.
8. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative Stress, Inflammation, and Cancer: How are They Linked? Free Radical Biology and Medicine 2010; 49(11): 1603-1616.
9. Kaur GJ, Arora DS. Antibacterial and Phytochemical Screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complementary and Alternative Medicine 2009; 9(30): 01-10.
10. Newman DJ, Cragg GM. Natural Products as Sources of New Drugs over the Last 25 Years. Journal of Natural Products 2007; 70(3): 461-477.
11. Braga R. Plantas do nordeste, especialmente do Ceará. 3rd edition. ESAM (Coleção Mossoroense), Fortaleza, 1976.
12. Hosamani KM. Fatty acids in seed oil from Turnera ulmifolia. Phytochemistry 1993; 34(5):1363-1365.
13. Sethi P. Antibacterial activity of aqueous extract of the leaves of Turnera ulmifolia Linn. (Turneraceae). Advance Research in Pharmaceuticals Biologicals 2011; 1(2): 101-104.
14. Gracioso JS, Vilegas W, Hiruma-Lima CA, Souza Brito AR: Effects of tea from Turnera ulmifolia L. on mouse gastric mucosa support the Turneraceae as a new source of antiulcerogenic drugs. Biol Pharmac Bull 2002; 25(4):487-491.
15. Nascimento MA, Silva AK, Franca LC, Quignard EL, Lopez JA, Almeida MG: Turnera ulmifolia L. (Turneraceae): Preliminary study of its antioxidant activity. Biores. Technol. 2006; 97(12):1387-1391.
16. Jana S, Deb JK: Molecular understanding of aminoglycoside action and resistance. Appl. Microbiol Biotechnol 2006; 70:140-150.
17. Smith E, Williamson M, Wareham N, Kaatz G, Gibbons S: Antibacterial and modulators of bacterial resistance from the immature cones of Chamaecyparis lawsoniana. Phytochemistry 2007; 68:210-217.
18. Vimalraj TR, Saravanakumar S, Vadivel S, Ramesh S, Thejomoorthy P. Antibcaterial effects of Cassia fistula extracts on pathogenic bacteria of veterinary importance. Tamilnadu J. Veter. Anim. Sci. 2009; 5:109–1.
19. Thirumalaisamy R, Gowrishankar J, Suganthapriya S, Prakash B, Ashok Kumar L. Genetic variability in Morus alba L by Biochemical and Bioassay Methods for increased Silk Productivity. J Biomed Sci and Res. 2009; 1(1): 11-18.
20. Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958; 181: 1199-1200.
21. Yildirim A, Mavi A, Oktay M. Comparison of antioxidant and antimicrobial activities of Tiliaargentea Desf ex D.C., sage (Salvia triloba I.), and black tea (Camellia sinensis) extracts. J. Agri. Food Che. 2001; 48: 530-534.
22. Kim TU, Gu BG, Jeong JY, Byun SM, Shin YC. Purification and characterization of a maltotetraose-forming alkaline µ-amylase from an alkalophilic Bacillus strain, GM8901. Applied and Environmental Microbiology 1995; 61(8): 3105-3112.
23. Henrique DM, Coutinho José GM, Costa Edeltrudes O, LimaVivyanne S, Falcão-Silva, José P Siqueira Júnior. BMC Complementary and Alternative Medicine 2009; 9(13): 9-13.
24. Hosamani KM. Fatty acids in seed oil from Turnera ulmifolia. Phytochemistry 1993; 34 (5):1363-1365.
25. Kadam Balaji, Manasa G, Amarender Reddy A, Nagaraju M, Srikanth T, Saarangi Ramesh. Evaluation of In vitro antioxidant and cytotoxicity activity of aqueous extract of Pergularia daemia. Sch. Acad. J. Pharm. 2013; 2(2):125-129.
26. Ebadi M. Pharmacodynamic basis of Herbal Medicines, CRC Press, Washington DC, 2002, pp. 86.
27. Jayaprakash GK, RP, Singh KK, Sakariah. Antioxidant activity of grape seed extracts on peroxidation models in vitro. J. Agri. Food Chem. 2001; 55: 1018.
28. Yang JH, Lin HC, Mau JL. Antioxidant properties of several commercial mushrooms. Food Chem. 2002; 77: 229-235.
Published
01-10-2014
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“ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC CRUDE EXTRACTS OF TURNERA ULMIFOLIA L”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 4, Oct. 2014, pp. 329-33, https://doi.org/10.25004/IJPSDR.2014.060410.
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How to Cite
“ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC CRUDE EXTRACTS OF TURNERA ULMIFOLIA L”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 6, no. 4, Oct. 2014, pp. 329-33, https://doi.org/10.25004/IJPSDR.2014.060410.
