SIMULTANEOUS ESTIMATION OF GUGGULSTERONE E, GUGGULSTERONE Z, KBA, AKBA, WITHAFERIN A AND 6-GINGEROL BY USING HPLC-DAD METHOD FROM ARIFLEX TABLET FORMULATION
Abstract
Simple, accurate, precise, sensitive and validated HPLC method was developed for simultaneous determination of Guggulsterone E (GE), Guggulsterone Z (GZ), 11-keto-β-boswellic acid (KBA), 3- Acetyl 11-keto-β-boswellic acid (AKBA), Withaferin A (WA) and 6-Gingerol (GNRL) in Ariflex tablet. The gradient RP-HPLC analysis was performed on Inertsil ODS-3V C18 column (250 × 4.6 mm id), using a mobile phase consisting of 0.1% Ortho-phosphoric acid and mixture of acetonitrile: Methanol (50: 50 v/v) in solvent gradient elution for 60 min at a flow rate of 1.0 mL min−1. Quantification was carried out using a photodiode array detector at 225nm. The employment of diode array detector allowed selectivity confirmation by peak purity evaluation. The method was validated according to International Conference on Harmonization guidelines. No chromatographic interference from tablet excipients was found and hence this method is applicable for simultaneous determination of GE, GZ, KBA, AKBA, WA and GNRL in Ariflex Tablet.
Keywords:
6-Gingerol, Boswelic acid, Ariflex Tablet, Guggulsterone, Withaferine-A, OsteoarthritisDOI
https://doi.org/10.25004/IJPSDR.2015.070116References
2. Hochberg MC, Altman RD, Brandt KD, Clark, BM, Dieppe PA, Griffin MR. Guidelines for the medical management of osteoarthritis. Osteoarthritis of the hip. American College of Rheumatology. Arthritis Rheum. 1995; 38(1):1535-40
3. Felson DT, Lawrence RC, Hochberg M, Mc Alindon T, Dieppe PA, Minor MA. Osteoarthritis: Treatment approaches. Ann Intern Med. 2000; 133(2):726-37.
4. Peat G, Duncan RC, Wood LR, Thomas E, Muller S. Clinical features of symptomatic patellofemoral joint osteoarthritis. Arthritis Res Ther. 2012; 14:R63.
5. Holderbaum D, Haqqi TM, Moskowitz RW. Genetics and osteoarthritis: Exposing the iceberg. Arthritis Rheum. 1994; 42:397-405.
6. Felson DT, Neogi T. Osteoarthritis: Is it a disease of cartilage or of bone? Arthritis Rheum. 2004; 50:341-4
7. Papaioannou NA, Triantafillopoulos IK, Khaldi L, Krallis N, Galanos A, Lyritis GP. Effect of calcitonin in early and late stages of experimentally induced osteoarthritis. A histomorphometric study. Osteoarthr Cartil. 2007; 07(15):386-95
8. Felson DT, Lawrence RC, Dieppe PA, Hirsch R, Helmick CG, Jordan JM. Osteoarthritis: The disease and its risk factors. Annals of Internal Medicine 2000; 133(1):635-46
9. Hochberg MC, Altman RD, Brandt KD, Clark BM, Dieppe PA, Griffin MR. Guidelines for the medical management of osteoarthritis. Osteoarthritis of the knee. American College of Rheumatology. Arthritis Rheum. 1995; 38(2):1541-6.
10. International Conference on Harmonization. Q2B: Validation of analytical procedures. US FDA Fed Reg. 97; 62:27463‑7.
Published
Abstract Display: 339 
PDF Downloads: 368 
