DETERMINATION OF THE RUTIN, QUERCETIN AND KAEMPFEROL CONTENTS IN A GHANAIAN POLYHERBAL FORMULATION (EAF-2011) AND ITS RAW MATERIALS USING A SIMULTANEOUS RP-HPLC METHOD
Abstract
Quantitative chemical fingerprinting of herbal medicines provides an avenue for addressing the challenge with their quality. In this report, chemical markers that could be labelled as active markers in the polyherbal product and its component raw materials were assayed using a simultaneous RP-HPLC method. These markers were rutin (RU), quercetin (QE) and kaempferol (KA) which are well known for their antimicrobial activities. The quantitative assay of the markers using a validated HPLC method showed the herbal product (EAF-2011) contained 8.6810% (w/w) of rutin, 0.2670% (w/w) of quercetin and 0.0610% (w/w) of kaempferol. At least two (2) of these flavonoids were detected in each of the component raw plant materials: Psidium guajava [(RU-8.5860% (w/w); QE-0.5430% (w/w); KA - 0.1003% (w/w)], Alchornea cordifolia [RU-2.5540% (w/w); QE-0.0536% (w/w)], Zanthoxylum zanthoxyloides [RU-5.8060 % (w/w); KA-0.0174% (w/w)], Tridax procumbens [RU-2.4390% (w/w); QE-0.1040% (w/w); KA-0.0510% (w/w)] and Eugenia caryophyllata [QE-0.4080% (w/w); KA-0.0771% (w/w)]. The results obtained indicate that the HPLC method, selected chemical markers and chromatograms produced are ideal for the quality control of the product and its component raw materials.
Keywords:
Herbal medicines, Polyherbal product, Quality Control, RP-HPLC, StandardisationDOI
https://doi.org/10.25004/IJPSDR.2015.070117References
2. Yi-Zeng L, Peishan X, Kelvin C. Quality control of herbal medicines. J Chromatography B 2004; 812: 53-70.
3. Songlin L, Quanbin H, Chunfeng Q, Jingzheng S, Chuen L, Cheng X, Hongxi X. Chemical markers for the quality control of herbal medicines: an overview. Chinese Medicine 2008; 3: 7-23.
4. Choudhary N, Sekhon SB. An overview of advances in the standardization of herbal drugs. J Pharm Educ Res. 2011; 2(2): 55.
5. Dickson RA, Mensah MLK, Thomford KP, Annan K, Mills-Robertson FC, Appiah AA, Quarshie O, Antwi S, Kaminta S, Edoh D. Quality and safety evaluation of a Ghanaian polyherbal product EAF-2011 for the management of superficial mycoses. Int J Med Biomed Res. 2013; 2(2): 91-102.
6. ICH, Validation of Analytical Procedures: Methodology. ICH Q2b. 1997, FDA. p. 27463.
7. EMEA, Reflection paper on markers used for quantitative and qualitative analysis of herbal medicinal products and traditional herbal medicinal products. 2008, European Medicine Agency: London.
8. Abad JM, Ansuategui M, Bermejo P. Active antifungal substances from natural sources. ARKIVOC 2007; vii: 116-145.
9. Tim Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J of Antimicrob Agents 2005; 26: 343-356.
10. Dubey S, Ganeshpurkar A, Bansal D, Dubey N. Experimental studies on bioactive potential of rutin. Chronicles of the Young Scientist 2013; 4: 153-157.
11. Han Y. Rutin has therapeutic effect on septic arthritis caused by Candida albicans. Int Immunopharmacol. 2009; 9: 207-11.
12. Singh M, Govindarajan R, Singh Rawat AK, Khare PB. Antimicrobial Flavonoid Rutin from Pteris vittata L. against pathogenic gastrointestinal microflora. American Fern Journal 2008; 98: 98-103.
13. Singh D, Sharma SK, Rani R, Mishra S, Sharma RA. Kaempferol-7-O-Glucoside and their Antimicrobial Screening isolate from Cassia renigera Wall. International Journal of Pharmaceutical and Clinical Research 2011; 3(2): 30-34.
14. Tatsimo SJN, Tamokou JD, Havyarimana L, Csupor D, Forgo P, Hohmann J, Kuiate JR, Tane P. Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum. BMC Research Notes 2012; 5: 158.
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