ANTI-TUMOR PROPERTY OF PLANT PROTEASE INHIBITOR ON HUMAN CHRONIC MYELOID LEUKEMIA CELLS
Abstract
Many Plant Protease inhibitors are found to have effects of tumor suppression in certain cell lines. In this work attempt has been made to determine anti-tumor/apoptotic property of trypsin inhibitor protein (WbTI2) from plant Psophocarpus tetragonolobus (L.)D.C. (winged bean) on human chronic myeloid leukemia cells (K562). Pure winged bean trypsin inhibitor protein was purified by affinity chromatography from recombinant molecule and human chronic myeloid leukemia cells were treated by it. Then viability of the treated cells were determined by simple microscopic study, MTT assay and flow cytometric analysis. All results revealed significant effect of WbTI2 on the viability of the human chronic myeloid leukemia cells. The IC50 value was determined to be 5µg/ml in MTT assay. The data was found to be statistically significant.
Keywords:
tumor suppression, apoptotic property, winged bean trypsin inhibitor, Chronic Myeloid Leukemia cellDOI
https://doi.org/10.25004/IJPSDR.2022.140402References
Deininger MW, Goldman JM, Melo JV- The molecular biology of chronic myeloid leukemia. Blood. (2000); 96(10), 3343-3356; doi:10.1182/blood.V96.10.3343
Bhutani N- The chronic myeloid leukemia in India: A Review. International Journal of Science and healthcare research. ( 2020); 5(1); doi: https://doi.org/10.52403/ijshr
Alvarez RH, Kantarjian H, Cortes JE -The biology of chronic myelogenous leukemia: implications for imatinib therapy. Seminars in Hematology. (2007); 44, 4-S14; doi: 10.1053/j.seminhematol.2006.12.007.
Melo JV, Chuah C - Resistance to imatinib mesylate in chronic myeloid leukemia. Cancer Letters. (2007); 249, 121-13; Melo JV, Chuah C (2007) Resistance to imatinib mesylate in chronic myeloid leukemia. Cancer Letters; 249, 121-13; doi: 10.1016/j.canlet.2006.07.010
Corbin AS, Agarwal A, Loriaux M, Cortes J, Dininger MW, Druker BJ-Human Chronic myeloid leukemia stem cells are insensitive to imatinib despite inhibition of BCR-ABL activity. The journal of clinical investigation. (2011); 121(1), 396-40; doi: 10.1172/JCI35721.
Perl A, Carrol M- BCR-ABL kinase is dead; long live CML stem cell. The Journal of Clinical Investigation. (2011); 121(1), 22-25; doi: 10.1172/JCI43605
Marlin D, Bazeos A, Mahon FX, Eliasson L, Milojkovic D, Bua M, Apperley JF, Szydlo R, Desai R, Kozlowski K, Paliompeis C, Latham V, Foroni L, Molimard M, Reid A, Rezvani K, de Lavallade H, Guallar C, Goldman J, Khorashad JS- Adherence is the critical factor for achieving molecular responses in patients with chronic myeloid leukemia who achieve complete cytogenetic responses on imatinib. Journal of Clinical Oncology. (2010); 10; 28(14), 2381-2388; doi: 10.1200/JCO.2009.26.3087.
Ibrahim AR, Eliasson L, Apperley JF, Milojkovic D, Bua M, Szydlo R, Mahon F X, Kozlowski K, Paliompeis C, Foroni L, Khorashad JS, Bazeos A, Molimard M, Reid A, Rezvani K, Gerrard G, Goldman J, Marin D- Poor adherence is the main reason for loss of CCyR and imatinib failure for chronic myeloid leukemia patients on long-term therapy. Blood. (2011); 117(14), 3733-3736; doi: 10.1182/blood-2010-10-309807.
Pandey R, Patil N, Rao M-Proteases and protease inhibitors: Implications in antitumorigenesis and drug development. International Journal of Human Genetics. (2007, and Published online: 04 Sep 2017); 7(1), 67-82; doi: 10.1080/09723757.2007.11885986
Raj D (Ed) - Protease Inhibitors. Functional Plant Science and Biotechnology. (Special Issue 2012) 6 ,107
Suzuki K, Yano T, Sadzuka Y, Sugiyama T, Seki T, Asano R. -Restoration of connexin 43 by Bowman-Birk protease inhibitor in M5076 bearing mice. Oncology Reports. (2005); 13(6), 1247-1250
Kennedy AR, Billings PC, Wan XS, Newberne PM- Effects of Bow-man-Birk inhibitor on rat colon carcinogenesis. Nutrition and Cancer. (2002); 43(2), 174-186; doi: 10.1207/S15327914NC432_8.
Tang MX, Asamoto M, Ogawa K, Naiki-Ito A, Sato S, Takahasi S, Shirai T.-Induction of apoptosis in the LNCap human prostrate carcinoma cell line and prostrate adenocarcinoma of SV40T antigen transgenic rats by the Bowman-Birk inhibitor. Pathology International. (2009); 59(11), 790-796; doi: 10.1111/j.1440-1827.2009.02445.x.
Caccialupi P, Ceci LR, Siciliano RA, Pignone D, Clemente A, Sonnante G.-Bowman-Birk inhibitors in lentil: terologous expression, functional characterization and anti-proliferative properties in human colon cancer cells. Food Chemistry. (2010); 120, 1058-1066
Park SS, Obha H -Suppressive activity of protease inhibitors from buck-wheat seeds against human T-acute phoblastic leukemia cell lines. Applied Biochemistry Biotechnology. (2004); 117(2), 65-74; doi: 10.1385/abab: 117:2:065.
Li YY, Zhang Z, Wang ZH, Wang HW, Zhang L, Zhu L- rBTI induces apoptosis in human solid tumor cell lines by loss in mitochondrial transmembrane potential and caspase activation. Toxicology Letters. (2009); 189(2), 166-175; doi: 10.1016/j.toxlet.2009.05.018.
Bhattacharjee N, Banerjee S, Dutta SK- Cloning, expression and mutational studies of a trypsin inhibitor that retains activity even after cyanogen bromide digestion. Protein expression and purification (Elsevier). (2014); 96: 26-31, doi:10.1016/j.pep.2014.01.013.
Fenteany G, Schreiber SL- Specific inhibition of the chymotrypsin-like activity of the proteasome induces a bipolar morphology in neuroblastoma cells. Chem. Biol. (1996); 3(11); 905–912; doi: 10.1016/s1074-5521(96)90179-9.
Yamaue H, Tanimura H, Noguchi K, Hotta T, Tani M, Tsunoda T, Iwahashi M, Tamai M, Iwakura S -Chemo sensitivity testing of fresh human gastric cancer with highly purified tumor cells using the MTT assay. British Journal of Cancer (1992); 66, 794-799
Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C -A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V. Journal of Immunological Methods;(1995), 184, 39-51.
Published
Abstract Display: 611 
PDF Downloads: 519 
