IN VITRO STUDIES ON THE EFFECT OF PROSOPIS CINERARIA ON THE MOTILITY AND ACETYLCHOLINESTERASE OF COTYLOPHORON COTYLOPHORUM
Abstract
Paramphistomosis caused by the paramphistome Cotylophoron cotylophorum constitute a major group of disease in domestic ruminants. Chemical control of helminths coupled with improved management has important worm control strategy throughout the world. However, increasing problems of development of resistance in helminths against anthelmintics have led to the proposal of screening of medicinal plants for their anthelmintic activity. In the present investigation, the anthelmintic efficacy of bark of Prosopis cineraria was assessed based on its effect on the motility and on the enzyme acetylcholinesterase (AChE) of Cotylophoron cotylophorum. The flukes were exposed to five different concentrations (1, 2, 3, 4 and 5%) of ethanol extract of bark of Prosopis cineraria (PcEE) for 2, 4 and 8 h. The motility response of the drug-treated parasites was quantitatively measured with the aid of Electronic Micromotility Meter (EMM). The electronic measurement of the motility of the drug-treated parasites clearly indicates the direct impact of the drugs on the motility of the parasite. Maximum level of inhibition of the motility response of the parasites was observed at 5% concentration after 8 h of exposure of PcEE. AChE was assessed. PcEE significantly inhibited the activity of AChE. As, AChE plays an important role in neurotransmission, inhibition of AChE results in muscular paralysis of parasites which loose its “biochemical hold-fast” and gets eliminated from the host. PcEE possesses a remarkable anthelmintic activity against C. cotylophorum. It may serve as an alternative for anthelmintic chemotherapeutic agents and could be used in a safe and eco friendly manner. However, this study warrants further in vivo studies for practical utility.
Keywords:
Cotylophoron cotylophorum, Prosopis cineraria, Acetylcholinesterase (AChE), Electronic Micromotility Meter (EMM)DOI
https://doi.org/10.25004/IJPSDR.2015.070306References
2. Hassan SS, Kaur K, Joshi K, Juyal PD. Epidemiology of paramphistomosis in domestic ruminants in different districts of Punjab and other adjoining areas. J. Vet. Parasitol. 2005; 19(1): 43-46.
3. Veerakumari L. In vitro studies on the effect of some anthelmintics on Cotylophoron cotylophorum. (Fischoeder, 1901) (Digenea; Paramphistomidae). A structural and biochemical analysis. Ph.D, thesis University of Madras, Chennai. India, 1996.
4. Athanasiadou S, Githiori S, Kyriazakis I. Medicinal plants for helminth parasite control: facts and fiction. Animal. 2007; 1(9): 1392-1400.
5. Khatri A, Rathore A, Patil UK. Assessment of anthelmintic Activity of the Prosopis cineraria (Linn). Druce bark. Indian J Nat Prod Res. 2011; Vol. 2(4): 512-514.
6. Jabbar A, Raza MA, Iqbal Z, Khan MN. An inventory of the Ethnobotanicals used as Anthelmintics in the southern punjab (pakistan). J Ethnopharmacol. 2006; 108(1): 152-4.
7. Kumar A, Yadav SK, Singh S, Pandeya SN. Analgesic activity of ethanolic extract of roots of prosopis cineraria (L.) Druce. J Appl Pharmaceut Sci. 2011; 01(08): 158-160.
8. Robertson S, Narayanan N, Kapoor BR. Antitumor activity of Prosopis cineraria (L.) Druce against agrlich ascites carcinoma- induced mice. Nat Prod Res. 2011; 25(8): 857-862.
9. Velmurugan V, Arunachalam G, Ravichandran V. Anthelmintic potential of Prosopis cineraria (Linn.) druce stems barks. Asian J Plant Sci Res. 2011; 1(2): 88-91.
10. Niezen JH, Waghorn TS, Charleston WAG, Waghorn GC. Growth and gastrointestinal nematode parasitism in lambs grazing either Lucerne (Medicago sativa) or Sulla (Hedysarum coronarium) which contains condensed tannins. J Agri Sci. 1995; 125: 281-289.
11. Veerakumari L. Micromotility meter: an instrument evaluates the efficacy of anthelmintic drugs. Fourteenth National Congress of Vet Parasitol. 2003; Pp. 87.
12. Lee DL. Why does some Nematode Parasites of Alimentary tract secrete Acetylcholinesterase? Int J Parasitol. 1996; 26: 499-508.
13. Van den Bossche HP. The Molecular Basis of Anthelmintic action. In: Biochemistry of Parasites and Host: Parasite Relationships van den Bossche H, (Ed.). Amsterdam, New York, 1976; 553-572.
14. Coles GC. The Biochemical mode of action of some modern anthelmintics. Pesti sci. 1977; 8: 536-543.
15. Manger BR. Anthelmintics veterinary applied pharmacology and therapeutics. (Brander GC, pugh Dm, Bywater RJ, Jenkins WL, eds) ELBS/ Bailliere, Tindall, 1991; 513-548.
16. Ogilvie M, Jones VE. Nippostrongylus brasilienses: a review of immunity and the host/parasite relationship in the rat. Exp Parasitol. 1971; 29: 138-177.
17. Fischoeder F. Die Paramphistomiden der Saiigethiere. Zool. Anx. 1901; 24: 365-375.
18. Ellman GL, Courtney KD, Andres VJr, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961; 7: 88–95.
19. Lowry OH, Rosebrough NJ, Farr A, Randall RJ. Protein measurement with folinphenol reagent. J Biol Chem. 1951; 193: 265-275.
20. Fairweather I, Holmes SD, Threadgold, LT. Fasciola hepatica: Motility response to fasciolicides in vitro. Exp Parasitol. 1984; 57(3): 209-224.
21. Holmes SD, Fairweather I. Fasciola hepatica: The effects of neuropharmacological agents upon in vitro motility. Exp Parasitol. 1984; 58: 194-208.
22. Kumar D, Tripathi HC. Paralytic effect of fasciolicidal agents on rhythmic motility of Fasciola gigantica. Indian J Animal Sci. 1998; 68: 1126-1129.
23. Veerakumari L, Priya P. In Vitro effect of Azadirachtin on the motility and Acetylcholinesterase activity of Cotylophoron cotylophorum (Fishoeder, 1905). J Vet parasitol. 2006; 20: 1-5.
24. Barragry T. Anthelmintics - A review: Part II (1984). New Zealand. Vet J. 32: 191-199.
25. Sharma RK, Singh K, Saxena KK. Effect of parbendazole and piperazine adipate on the activity of some enzymes of Ascardia galli and Heterakis gallinae. Vet Parasitol. 1987; 24: 211-220.
26. Philipp M. Acetylcholinesterase secreted by intestinal nematodes: a reinterpretation of its putative role of “biochemical holdfast”: Trans. Royal Soc. Trop Med Hygiene, 1984; 78: 138-139.
27. Rhoads ML. Secretory cholinesterases of nematodes: possible functions in the host-parasite relationships. Trop Vet. 1984; 2: 3-10.
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