Evaluation of anti-asthmatic activity of aqueous extract of bark of Prosopis cineraria (L.) Druce against milk induced leukocytosis and eosinophilia
Abstract
The present investigation was conducted to evaluate the anti-asthmatic activity of aqueous extract of the bark of Prosopis cineraria (L.) Druce to validate its traditional and ethnopharmacological use. Aqueous extract of the bark of P. cineraria (L.) Druce at doses of 100 and 200 mg/kg b.wt i.p was evaluated for the assessment of its anti-asthmatic activity using milk induced leukocytosis and eosinophilia, oxidative stress analysis in the liver and kidney, along with histopathological changes in the trachea of mice. The results of the present investigation showed that aqueous extract of bark of P. cineraria at (100 and 200 mg/kg b.wt, i.p) significantly decreased milk induced leukocytosis and eosinophilia, was effective towards restoration of enhanced levels of lipid peroxidation and reduced levels of GSH and SOD in the liver and kidney, and showed a reduction in the thickness of the tracheal wall and cartilage along with inflammatory cells in mice in a dose-dependent manner when compared with the control group. It can be concluded that aqueous extract of the bark of P. cineraria (L.) Druce possesses anti-asthmatic activity.
Keywords:
Prosopis cineraria, leukocytosis, eosinophilia, oxidative stress.DOI
https://doi.org/10.25004/IJPSDR.2022.140505References
Clarke R, Lundy FT, McGarvey L. Herbal treatment in asthma and COPD-current evidence. Clin. Phytosci. 2015; 1(4): 1-7.
Mali RG, Dhake AS. A review on herbal antiasthmatics. Orient. Pharm. Exp. Med. 2011; 11: 77-90.
Taur DJ, Patil RY. Anti-asthmatic activity of Ricinus communis L. Roots. Asian Pac. J. Trop. Biomed. 2011; 1(1):13-16.
Kaul RN. Trees or grass lands in the Rajasthan- Old problems and new approaches, Indian For. 1967; 93: 434-435.
Burkart A. A monograph of the genus Prosopis (Leguminosae subfam. Mimosoideae). Part 1 and 2. Catalogue of the recognized species of Prosopis. J. Arnold Arbor. 1976; 57(3): 219-249, 450-526.
Mann HS, Shankarnarayan KA. The role of Prosopis cineraria in an agropastoral system in western Rajasthan. In browse in Africa symposia, H. N. LeHouerou (ed.), Addis Ababa, Ethiopia. 1980; pp. 437-442.
Khatri A, Rathore A, Patil UK. Prosopis cineraria (L.) Druce: A boon plant of desert- An overview. Int. J. Biomed. Adv. Res. 2010; 1(5): 141-149.
Vyas S, Pandya D, Mankad A. A review on Prosopis cineraria as an important plant of arid regions of India. Int. J. Multidiscip. Res. 2020; 6(3): 1-6.
Singh P, Bangarwa KS, Dhillon RS. Studies on Phenology and Reproductive Biology of Khejri [Prosopis cineraria (L.) Druce]. Indian J. Agric. Res. 2021; 55(1): 110-114.
Fernanada BAP, Cibele MCPG, Patricia PA, Ione S. Protective action of hexane crude extract of Pterodon emarginatus fruits against oxidative and nitrosative stress induced by acute exercise in rats. BMC Complementary Alter. Med. 2005; 5:17-21.
Sedalk J, Lindsay RH. Estimation of total protein bound and non-protein bound sulfhydryl groups in tissue with Ellman’s reagent. Anal. Biochem. 1968; 25:192-205.
Madesh M, Balasubramanian KA. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochem. Biophys. 1998; 35(3):184-188.
Lillie RD. Histopathologic technique and practical histochemistry. McGraw Hill Book Co. New York and London.1965;176.
Pathan AR, Vadnere G, Sabu M. Evaluation of antiasthmatic effect of Curcuma zedoaria rhizomes by using milk induced leukocytosis in mice. Neuro Pharmac. J. 2016; 1(1):13-17.
Banjarnahor SDS, Artanti N. Antioxidant properties of flavonoids. Med. J. Indones. 2014; 23(4): 239-244.
Rice-Evans, CA, Miller NJ, Paganga G. Structure–antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol. Med. 1996; 20: 933-956.
Gaschler MM, Stockwell BR. Lipid peroxidation in cell death. Biochem. Biophys. Res. Commun. 2017; 482(3): 419-425.
Wu G, Fang YZ, Yang S, Lupton JR, Turner ND. Glutathione metabolism and its implications for health. The J. Nutri. 2004; 134: 489-492.
Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J. Med. 2018; 54(4): 287-293.
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