STUDY ON CYTOTOXIC EFFECT OF ROOT AND CALLUS EXTRACTS OF MYXOPYRUM SMILACIFOLIUM BLUME
Abstract
Myxopyrum smilacifolium is a large woody climbing shrub belonging to the family Oleaceae which is known for many medicinal properties. In the present study, initially a short term cytotoxic study (3 hours) with hepatocarcinoma cell line HepG2 using Trypan Blue Assay was carried out against methanolic extracts of root and callus and found an appreciable result for both extracts. Then the study was extended to the same cell line employing MTT assay. In this procedure extracts were subjected to 24 hours treatment and cytotoxicity was measured using a colorimetric method based on the ability of metabolic active cells to cleave the yellow tetrazolium salt MTT to an insoluble purple formazan crystal. A concentration dependent activity was observed in both Trypan Blue as well as MTT assay. The results showed that both the extracts possess potent cytotoxic activity. IC50 value from MTT assay was found to be 63.75µg/l and 98.75µg/l for root and callus extracts respectively. To the best of our knowledge, this is the first study to demonstrate the cytotoxic potential of callus extracts of Myxopyrum smilacifolium Blume.
Keywords:
Callus, HepG2 cells, Trypan Blue, MTTDOI
https://doi.org/10.25004/IJPSDR.2015.070407References
2. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Af.r .J Biotechnol. 2005; 4:685-688.
3. Xiaomei Ma, Herbert Yu. Global Burden of Cancer. Yale J Biol Med. 2007; 79(3-4): 85-94.
4. Cragg GM, Newman DJ. Plants as a source of anticancer agents. Journal of Ethnopharmacology 2005; 100:72-79.
5. Reed JC, Pellecchia M. Apoptosis-based therapies for hematologic malignancies. Blood. 2005; 106: 408-418.
6. Vaidhyrathnam PS. Warrier Indian medicinal plants- a compendium of species. Arya vaidya sala Kottakkal. 1996; 4: 88.
7. Gopalakrishnan S, Rajameena R, Vadivel E. Antimicrobial activity of leaves of Myxopyrum serratulum A. W. Hill. Int. J. Pharm. Sci. Drug Res. 2012; 4(1): 31-34.
8. Sudharmini D, Nair AS. Antimicrobial Studies of Triterpenoid Fractions from Myxopyrum smilacifolium Blume. Ethnobotanical Leaflets. 2008; 12: 912-15.
9. Franzyk H, Jensen SR, Olsen CE. Iridoid glucosides from Myxopyrum smilacifolium. J Nat Prod. 2001; 64: 632-633.
10. Bhojwani SS, Razdan MK. Plant tissue culture: Theory and Practice: Developments in crop science. Elsevier, Amsterdam. 1996: 71-90.
11. Fowler MR, Rayns FW, Hunte CF. The language and aims of plant cell and tissue culture. In vitro Cultivation of Plant Cells. Butterworth-Heinemann Ltd, Oxford. 1993: 1-18.
12. Arung ET, Shimizu K, Kondo R. Inhibitory effect of artocarpanone from Artocarpus heterophyllus on melanin biosynthesis. Biol Pharm Bull. 2006; 29: 1966-1969.
13. Patel PR, Raval BP, Karanth HA. Potent antitumor activity of Rubia cordifolia. International Journal of Phytomedicine 2010; 2: 44-46.
14. Praveen RP, Nair AS. Preliminary Phytochemical Screening of Root Extracts of Myxopyrum smilacifolium Blume. Asian Journal of Biomedical and Pharmaceutical Sciences 2014; 04(38):41-45.
15. Praveen RP, Nair AS. Antioxidant potential of methanolic extract of root, callus and fruit extracts of Myxopyrum smilacifolium Blume. International Journal of Biological & Pharmaceutical Research 2015; 6(1): 51-55.
Published
Abstract Display: 413 
PDF Downloads: 486 
