CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF IXORA COCCINEA METHANOLIC FLOWER EXTRACT
Abstract
Ixora coccinea (R.) belonging to the Rubiaceae family is an ornamental plant claimed for the management of oral cancer and one of ingredients in curing different ailments. The present investigation was carried out to determine the chemical composition of Ixora coccinea methanolic flower extract using HPLC, HPTLC and Gas Chromatography–Mass Spectrometry technique. HPLC analysis of Ixora cocccinea methanolic flower extract revealed the presence of Biochin A, Myricetin, Quercetin, Rutin, Diadzein and formononetin, HPTLC fingerprint revealed the presence of ursolic acid and GC-MS with 24 phytochemicals among which some are of biological importance. The result of this study offers a platform for using Ixora coccinea pharmacologically.
Keywords:
Ixora cocccinea, HPLC, HPTLC, GC-MS techniques, Quercetin, Ursolic acid.DOI
https://doi.org/10.25004/IJPSDR.2011.030411References
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2. Yang LW, Wu DH, Tang X, Pang W, Wang XR, Ma Y, Su WW. Chromatographic separation of Lagenaria siceraria fruit extract by HPTLC. Journal of Chromatography A., 2005; 1070: 35–42.
3. Seethadevi B, Nair CRS, Paniker PV. Antimicrobial activity of leaves of Ixora coccinea. Indian Journal of Pharmaceutical Sciences, 1991; 53: 92
4. Annapurna J, Amarnath PVS, Amar Kumar D, Rama Krishna SV, Raghavan KV. Antimicrobial activity of Ixora coccinea flowers. Fitoterpia, 2003; 74: 291-293.
5. Latha PG, Panikkar KR. Chemoprotective effect of Ixora coccinea L.flowers on cisplatin induced toxicity in mice. Phytother Research, 2001; 15: 364-366.
6. Latha PG, Panikkar KR. Modulatory effects of Ixora coccinea flower on cyclophosphamide-induced toxicity in mice. Phytother Research, 1999; 13: 517-20.
7. Latha PG, Panikkar KR. Cytotoxic and antitumour principles from Ixora coccinea flowers. Cancer Letter, 1998; 130: 197-202.
8. Sohn KH, Lee HY, Chung HY, Young HS, Yi SY, Kim KW. Anti-angiogenic activity of triterpene acids. Cancer Letter, 1995; 94: 213–218.
9. Kim DK, Baek JH, Kang CM, Yoo MA, Sung JW, Chung HY, Kim ND, Choi YH, Lee SH, Kim KW. Apoptotic activity of ursolic acid may correlate with the inhibition of initiation of DNA replication. International journal of cancer, 2000; 87: 629–636.
10. Harmand PO, Duval R, Liagre B, Jayat-Vignoles C, Beneytout JL, Delage C, Simon A. Ursolic acid induces apoptosis through caspase-3 activation and cell cycle arrest in HaCat cells. International journal of oncology, 2003; 23: 105–112.
11. Huang MT, Ho CT, Wang ZY, Ferraro T, Lou YR, Stauber K, Ma W, Georgiadis C, Laskin JD, Conney AH. Inhibition of skin tumorigenesis by rosemary and its constituents carnosol and ursolic acid. Cancer Research, 1994; 54: 701–708.
Published
01-10-2011
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“CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF IXORA COCCINEA METHANOLIC FLOWER EXTRACT”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 4, Oct. 2011, pp. 327-30, https://doi.org/10.25004/IJPSDR.2011.030411.
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How to Cite
“CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF IXORA COCCINEA METHANOLIC FLOWER EXTRACT”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 3, no. 4, Oct. 2011, pp. 327-30, https://doi.org/10.25004/IJPSDR.2011.030411.
