ESSENTIAL OIL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF FLOWERS OF ACHILLEA FILIPENDULINA
Abstract
Achillea filipendulina is an aromatic plant of family Asteraceae with bright yellow colour flowers. The objective of the study was to extract the essential oil from the flowers of the selected plant using Clevenger apparatus, to analyse the chemical composition of the oil using GC-MS and to screen the essential oil for its antibacterial property against both gram-positive and gram-negative bacteria. GC-MS results revealed that the main chemical constituents present in the oil were trans 2,7 Dimethyl 4,6 octadien-2-ol (27.93%), borneol (21.44%) and santolina triene (7.13%). The results of agar well diffusion assay revealed that the oil showed antibacterial activity against all the tested bacteria except Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) of oil was determined against the sensitive bacteria. MIC values against Staphylococcus aureus and Escherichia coli were similar (0.312% v/v) and for Bacillus cereus, MIC value was found to be (0.156%). Results indicated the great antibacterial potential of oil of this plant against gram positive and gram negative bacteria.
Keywords:
Essential oil, GC-MS, Antibacterial.DOI
https://doi.org/10.25004/IJPSDR.2017.090405References
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27. Dormans HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 2000; 88(2):308-316.
28. Pirbalouti AG, Hamedi B, Malek PF, Rahimi E, Nasri NR. Inhibitory activity of Iranian endemic medicinal plants against Vibrio parahaemolyticus and Vibrio harveyi. J Med Plant Res. 2011; 5(32): 7049-7053.
29. Tripathi M, Chawla P, Upadhyay R, Trivedi S. Essential oils from Family Zingiberaceae- A Review. Int J Pharma Biosci. 2013; 4(4):149-162.
30. Knobloch K, Pauli A, Iberi B, Wegand H, Weis N. Antibacterial and antifungal properties of essential oil components. J Essent Oil Res.1989; 1:119-128.
31. Tabanca N, Kirimu N, Demirci B, Demirci F, Baser KHC. Composition and antimicrobial activity of the essential oils of Micromeria cristata subsp. phyrgia and the enantiomeric distribution of borneol. J Agric Food Chem 2001; 49:4300-4303.
32. Carson CF, Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J Appl Bacteriol. 1995; 74:264-269.
33. DeVincenzi M, Mancini E, Dessi MR. Monographs on botanical flavouring substances used in food PartV. Fitoterapia 1996; 67: 241-251.
34. Pattnaik S, Subramanyam VR, Bapaji M, Kole CR. Antibacterial and antifungal activity of aromatic constituents of essential oils. Microbios. 1997; 89:39-46.
35. Tzakou O, Pitarokili D, Chinou IB, Harvala C. Composition and antimicrobial activity of the essential oil of Salvia ringens. Planta Med. 2001; 67:81-83.
36. Viljoen A, Vuuren SV, Ernst E, Klepser M, Demirci B, Baser H, Wyk BEV. Osmitopsis asteriscoides (Asteraceae) – the antimicrobial and essential oil composition of a Cape-Dutch remedy. J Ethnopharmacol. 2003; 88:137-143.
2. Wilson CL, Droby CG. Microbial Food Contamination CRC Press, Boca Raton 2000.
3. Friedman M, Henika PR, Mandrell RE. Bacteriocidal activities of plant essential oils and some of their isolated constituents against Campilobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica. J Food Prot. 2002; 65:1545-1560.
4. Benayache S, Benayache F, Benyahia S. Leaf oils of some Eucalyptus species growing in Algeria. J Essent Oil Res. 2001; 13:210-213.
5. Benhassaini H, Benabderrahmane M, Chikhi K. Contribution to the assessment of the antiseptic activity of essential oils and oleoresin of Pistacia tial Atlas on some microbial sources: Candida albicans (ATC 20027), Candida albicans (20032) and Saccharomyces cerevisiae. Ethnopharmacol. 2003; 30:38-46.
6. Jeong MR, Park PB, Kim DH, Jang YS, Jeong HS, Choi SH. Essential oil prepared from Cymbopogon citrates Exerted an Antimicrobial Activity Against Plant Pathogenic and Medical Microorganisms. Mycobiology. 2009; 37(1):48-52.
7. Bhattacharjee I, Ghosh A, Chandra G. Antimicrobial Activity of the essential oil of Cestrum diurnum (L.) (Solanales: Solanaceae). Afr J Biotechnol. 2005; 4:371-374.
8. Doughari JH. Antibacterial activity of Tamarindus indica Linn. Trop J Pharm Res. 2006; 5:597-603.
9. Baratta MT, Dorman HJD, Deans SG, Figueiredo AC, Barroso JG, Ruberto G. Antimicrobial and Antioxidant properties of some commercial essential oils. Flavour Frag J. 1998; 13: 235-244.
10. Consentino S, Tuberoso CIG, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F. In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett Appl Microbiol. 1999; 29:130-135.
11. Bounatirou S, Smiti S, Miguel MG, Faleiro L, Rejeb MN, Neffati M, Costa MM, Figueiredo AC, Barroso JG, Pedro LG. Chemical composition, antioxidant and antimicrobial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. Food Chem. 2007; 105:146-155.
12. Rota C, Carraminana JJ, Burillo J, Herrera A. In vitro antimicrobial activity of essential oils from aromatic plants against selected food borne pathogens. J Food Prot. 2004; 67:1252-1256.
13. Saedinia S, Gohari AR, Mokhber-Dezfuli, Kiuchi F. A review on phytochemistry and medicinal properties of the genus Achillea. Daru. 2011; 19(3):173-186.
14. Stojanovic G, Radulovic N, Hashimoto T, Palic R. In vitro antimicrobial activity of extracts of four Achillea species: The Composition of Achillea clavennae L. (Asteraceae) extract. J Ethnopharmacol. 2005; 101(1): 185-190.
15. Sharopov FS, Setzer WN. Composition of the essential oil of Achillea filipendulina Lam. From Tajikistan. Der Pharma Chem. 2010; 2(6):134-138.
16. Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res. 2007; 21(4): 308-323
17. Wayne PA. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. Twenty first Informational supplement M100-S21. CLSI: 2011.
18. Rahimmalek M, Tabatabaei BE, Etemadi N, Goli SA, Arzani A, Zeinali H. Essential oil variation among and within six Achillea species transferred from different ecological regions in Iran to the field conditions. Inds Crops Prods. 2009; 29:348-355.
19. Chethan J, Sampath Kumara KK, Shailasree S, Prakash HS. Antioxidant, antibacterial and DNA protecting activity of selected medicinally important Asteraceae plants. Int J Pharm Pharm Sci. 2012; 4(2): 257-261.
20. Jenny A, Saha D, Paul S, Dutta M, Uddin MZ, Nath AK. Antibacterial activity of aerial part of extract of Elephantopus scaber Linn. Bull Pharm Res. 2012; 2(1):38-41.
21. Unlu M, Daferera D, Donmez E, Polissiou M, Tepe B, Sokmen A. Compositions and the in vitro antimicrobial activities of the essential oils of Achillea setacea and Achillea teretifolia (Compositae). J Ethnopharmacol. 2002; 83(1): 117-121.
22. Barel S, Segal R, Yashphe J. The antimicrobial activity of the essential oil from Achillea fragrantissima. J Ethnopharmacol. 1991; 33:187-191.
23. Kiyanpour V, Fakhari A, Asghari B, Yousefzadi M. Chemical composition and antibacterial activity of the essential oil of Achillea filipendulina (Asteraceae). Planta Med. 2011; 77(12): 49.
24. Jianu C, Misca C, Muntean SG, Gruia AT. Composition, antioxidant and antimicrobial activity of the essential oil of Achillea collina Becker growing wild in western Romania. Hem Ind. 2015; 69:381-386.
25. Fredj MB, Marzouk B, Chraief I, Boukef K, Marzouk Z. Analysis of Tunisian Ruta graveolens L. oils from Jemmal. J Food Agric Environ 2007; 5(1):52-55.
26. Gnanaprakasam AR, Manivachayam C, Sakthivel J, Venugopalan V. Phytochemical Analysis, Antibacterial and Antifungal Activities of Different Crude Extracts of Marine Red Alga Gracilaria corticata From the Gulf of Mannar South Coast, Navaladi, South India. Int J Pharm Sci Drug Res. 2017; 9:55-63.
27. Dormans HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 2000; 88(2):308-316.
28. Pirbalouti AG, Hamedi B, Malek PF, Rahimi E, Nasri NR. Inhibitory activity of Iranian endemic medicinal plants against Vibrio parahaemolyticus and Vibrio harveyi. J Med Plant Res. 2011; 5(32): 7049-7053.
29. Tripathi M, Chawla P, Upadhyay R, Trivedi S. Essential oils from Family Zingiberaceae- A Review. Int J Pharma Biosci. 2013; 4(4):149-162.
30. Knobloch K, Pauli A, Iberi B, Wegand H, Weis N. Antibacterial and antifungal properties of essential oil components. J Essent Oil Res.1989; 1:119-128.
31. Tabanca N, Kirimu N, Demirci B, Demirci F, Baser KHC. Composition and antimicrobial activity of the essential oils of Micromeria cristata subsp. phyrgia and the enantiomeric distribution of borneol. J Agric Food Chem 2001; 49:4300-4303.
32. Carson CF, Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J Appl Bacteriol. 1995; 74:264-269.
33. DeVincenzi M, Mancini E, Dessi MR. Monographs on botanical flavouring substances used in food PartV. Fitoterapia 1996; 67: 241-251.
34. Pattnaik S, Subramanyam VR, Bapaji M, Kole CR. Antibacterial and antifungal activity of aromatic constituents of essential oils. Microbios. 1997; 89:39-46.
35. Tzakou O, Pitarokili D, Chinou IB, Harvala C. Composition and antimicrobial activity of the essential oil of Salvia ringens. Planta Med. 2001; 67:81-83.
36. Viljoen A, Vuuren SV, Ernst E, Klepser M, Demirci B, Baser H, Wyk BEV. Osmitopsis asteriscoides (Asteraceae) – the antimicrobial and essential oil composition of a Cape-Dutch remedy. J Ethnopharmacol. 2003; 88:137-143.
Published
01-07-2017
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“ESSENTIAL OIL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF FLOWERS OF ACHILLEA FILIPENDULINA”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 9, no. 4, July 2017, pp. 182-6, https://doi.org/10.25004/IJPSDR.2017.090405.
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How to Cite
“ESSENTIAL OIL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF FLOWERS OF ACHILLEA FILIPENDULINA”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 9, no. 4, July 2017, pp. 182-6, https://doi.org/10.25004/IJPSDR.2017.090405.
