FORMULATION AND IN-VITRO EVALUATION OF POLYHERBAL MICRO-EMULGEL CONTAINING TINOSPORA CORDIFOLIA AND CURCUMIN FOR TREATMENT OF ARTHRITIS
Abstract
Arthritis is the condition which is associated with inflammation of a joint, pain, swelling, and stiffness. Drug delivery to the target site remains a challenge due to ineffective drug delivery system. An attempt has been made to formulate and evaluate micro-emulgel for the effective drug delivery in the treatment of Arthritis. Micro-emulgel was loaded with Curcumin and Tinospora cordifolia to enhance bioavailability of extracts which have been widely used in the treatment of arthritis. Micro-emulgel was prepared by emulsion-solvent diffusion method using carbopol 940P as a gelling agent. Micro-emulsion was formulated using Liquid paraffin oil as oil phase; Tween 80 and Span 20 as surfactant and co-surfactant respectively. FTIR studies proved the compatibility between drug, excipient and carbopol. The Prepared micro-emulgel was subjected to various parameters such as pH, rheological studies, spreadability, thermodynamic stability tests, drug content, electro conductivity, and in-vitro release studies. The pH of all formulations was found near to the skin pH value. Viscosity and spreadability of F1 optimized formulation was found to be 146.5×103 cPs and 2.24 g×cm. From the in vitro drug release study, it was revealed that sustained release of formulation last up to 18 hours. F1 formulation showed the highest drug release of Curcumin (92.37%) and Tinospora cordifolia (90.75%). SEM images showed the diameter of oil globules of Micro-emulgel were in range of 1.50 to 2.13µm. Drug release kinetics showed the zero order drug release from the optimized F1 formulation. From the stability studies, F1 formulations had an excellent physical stability.
Keywords:
Arthritis, Micro-emulgel, Curcumin, Tinospora cordifolia, topical gel, in-vitro release studiesDOI
https://doi.org/10.25004/IJPSDR.2016.080504References
2. Dubois N. Guidelines to formulate OTC drugs in topical emulsions with improved sensorial properties. Conference AAPS meeting, Atlanta. 2008.
3. Rieger M, Lachman L, Lieberman H, Kanig J. The Theory and Practice of Industrial Pharmacy. 3rd ed, PA Lea and Febiger: Philadelphia, 1986, 502-33.
4. Khullar R, Saini S, Seth N, Rana A. Emulgels: A surrogate approach for topically used hydrophobic drugs. International Journal of Pharmacy and Biological Sciences 2011; 1: 117-28.
5. Panwar AS, Upadhyay N, Bairagi M, Gujar S, Darwhekar GN, Jain DK. Emulgel: A Review. Asian J. of Pharm and Life Sci. 2011; 1(3): 333-343.
6. Jain A, Gautam SP, Gupta, Jain S. Development and characterization of Ketoconazole emulgel for topical drug delivery. Der Pharmacia Sinica. 2010; 1(3):221- 231.
7. H. Mohan. Textbook of Pathology. Edn 6. J.P. Brothers, New Delhi, 2013.
8. Csaki C, Mobasheri A, Shakibaei M. Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1beta-induced NF-kappaB-mediated inflammation and apoptosis. Arthritis Res Ther. 2009; 11(6):R165.
9. Mathy M, Sanchez C, Priem F, Henrotin Y. Curcumin inhibits interleukin-6, -8, nitric oxide and prostaglandin E2 synthesis by bovine chondrocytes. Osteoarthr Cartil/OARS. Osteoarthr Res Soc. 2007; 15.
10. Chopra A, Saluja M, Tillu G, et al. Comparable efficacy of standardized ayurveda formulation and hydroxychloroquine sulfate (HCQS) in the treatment of rheumatoid arthritis (RA): A randomized investigator-blind controlled study. Clin Rheumatol. 2012; 31:259–69.
11. Abiramasundari G, Sumalatha KR, Sreepriya M. Effects of Tinospora cordifolia (Menispermaceae) on the proliferation, osteogenic differentiation and mineralization of osteoblast model systems in vitro. J Ethnopharmacol. 2012; 141:474–80.
12. Cartensen JT. Modern pharmaceutics: Preformulation, edn 3, Vol. 72, Marcel Dekker, New York (NY), 1996, pp. 213-38.
13. Effionora A, Delly R, Harmita. Formulation and evaluation of gel and emulgel of chili extract as topical dosage forms. Int J Pharm Pharm Sci.2014; l 6(3): 13-16.
14. Panwar AS, Upadhyay N, Bairagi M, Gujar S, Darwhekar GN, Jain DK. Emulgel: A review. Asian J Pharm Life Sci. 2011; 1(3):333-343.
15. Ashara KC, Paun JS, Soniwala MM, Chavada JR, Mori NM. Micro-emulsion based emulgel: a novel topical drug delivery system. Asian Pacific Journal of Tropical Disease 2014; (1):S27-S32.
16. Barakat N, Fouad E, Elmedany A. Formulation design of indomethacin-loaded nanoemulsion for transdermal delivery. Pharm Anal Acta. 2011; S2:002.
17. Harika K, Subhashis Debnath, M Niranjan Babu. Formulation and Evaluation of Nanoemulsion of Amphotericin B. Int J Novl Trends Pharma Sci. 2015; 5(4): 114-122.
18. Kalra R, Mulik RSA, Badgujar L, Paradkar AR, Mahadik KR, Bodhankar SL. Development and characterization of micro-emulsion formulations for transdermal delivery of Aceclofenac: a research. IJDFR. 2010; 1(1): 359-386.
19. Nanjwade BK, Varia PJ, Kadam VT, Srichana T, Kamble MS. Development and Evaluation of Nanoemulsion of Repaglinide. JSMNN. 2013; 1(2):1-8.
20. Srilatha R, Aparna C, Srinivas P, Sadanandam M. Formulation, Evaluation and Characterization of Glipizide Nanoemulsion. AJPCR. 2013; 6 (2): 66-71.
Published
Abstract Display: 726 
PDF Downloads: 709 
