Evaluation of Cell Viability Effects and Antioxidant Activity of Caryota urens Linn. and Couroupita guianensis aublet Leaves in a Model of Ehrlich Ascites Carcinoma as a Xenograft model
Abstract
Plants used in traditional medicine have been identified as the primary source of anticancer agents. One of the cancer-related parameters is the cell viability parameter. Current study aimed to evaluate cell viability effects and antioxidant activity of Caryota urens Linn. and Couroupita guianensis aublet leaves using xenograft model by transplantation of ehrlich ascites carcinoma (EAC) cells into mice. Animals were randomly divided into seven groups of six animals each. For 10 days, EAC cells (2 × 106 cells/mouse) were injected i.p. into each mouse in each group except the normal control and vehicle control groups. The treatment drugs were compared with standard 5-fluorouracil. Group I received water as a control, group II received 0.9% normal saline, group III received 0.5% CMC, group IV received EAC cells as a model control group, group V received EAC cells with 5-flourouracil treatment, group VI received EAC cells with C. urens linn extract, and group VII received EAC cells with C. guianensis aublet. After 10 days of treatment, animals were sacrificed, ascitic fluid was collected for evaluation of cell viability effects, blood collected for hematological parameter estimation, and liver tissue was collected for histopathological study and evaluation of antioxidant activity. Study results of C. urens linn and C. guianensis aublet both shows significant positive effects on cell viability and antioxidant activity. In the study visible differences were observed in liver tissue of different groups. Disease control groups showed damaged liver cells, while the treatment group showed less damage than disease control. Both plants produced positive effects on cell viability and antioxidant activity, resulting in a decrease in viable cell count.
Keywords:
Cell viability effect, antioxidant activity, CaryotaUrens Linn, Couroupita guianensis Aublet, Ehrlich Ascites Carcinoma, Xenograft modelDOI
https://doi.org/10.25004/IJPSDR.2023.150206References
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global Cancer Statistics 2018. GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians 2018; 9: 68(6): 394–424.
De Vita VT, Samuel H, Steven AR. Cancer-Principles and Practice of Oncology, 7th ed., New York. Lippincott Williams and Wilkins 2005.
Rutman R J, Cantarow A, Paschkis KE. Studies in 2-acetylaminofluorene carcinogenesis. III. The utilization of uracil-2-C14 by preneoplastic rat liver and rat hepatoma. Cancer Research 1954; (2)1: 14(2):119–23.
Wohlhueter RM, McIvor RS, Plagemann PG. Facilitated transport of uracil and 5-fluorouracil, and permeation of orotic acid into cultured mammalian cells. Journal of Cellular Physiology 1980; (9)1: 104(3):309–19.
Thomas D, Zalcberg J. 5-fluorouracil: A pharmacological paradigm in the use of cytotoxics. Clinical and Experimental Pharmacology and Physiology 1998; 11: 25(11):887–95.
Noordhuis P, Holwerda U, Van der Wilt CL, Van Groeningen CJ, Smid K, Meijer S, et al. 5-Fluorouracil incorporation into RNA and DNA in relation to thymidylate synthase inhibition of human colorectal cancers. Annals of Oncology 2004; 15(7):1025–32.
Wohlhueter RM, McIvor RS, Plagemann PG. Facilitated transport of uracil and 5-fluorouracil, and permeation of orotic acid into cultured mammalian cells. Journal of Cellular Physiology 1980; 104(3):309–19.
Komarova EA, Gudkov AV. Suppression of p53: a new approach to overcome side effects of antitumor therapy. Biochemistry Biokhimiia. 2000; 65(1):41–8.
Cordier PY, Nau A, Ciccolini J, Oliver M, Mercier C, Lacarelle B, et al. 5-FU-induced neurotoxicity in cancer patients with profound DPD deficiency syndrome: a report of two cases. Cancer Chemotherapy and Pharmacology 2011; 68(3):823–6.
Yeh ET, Tong AT, Lenihan DJ, Yusuf SW, Swafford J, Champion C, Durand JB, Gibbs H, Zafarmand AA, Ewer MS. Cardiovascular complications of cancer therapy: diagnosis, pathogenesis, and management. Circulation 2004; 109(25):3122-31.
Gülçin İ, Büyükokuroǧlu M, Oktay M, Küfrevioǧlu Ö. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. Pallsiana (Lamb.) Holmboe. Journal of Ethnopharmacology 2003; 86(1):51-8.
Desai AG, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, Bedi YS, Taneja SC, Bhat HK. Medicinal plants and cancer chemoprevention. Curr Drug Metab 2008; 9(7):581-91.
Abd-Elhakim YM, El Bohi KM, Hassan SK, El Sayed S, Abd-Elmotal SM. Palliative effects of Moringa olifera ethanolic extract on hematoimmunologic impacts of melamine in rats. Food Chem Toxicol 2018; 114:1-10.
Mohammed HH, Ebraheim LLM, Shalaby MA. Camel milk rescues neurotoxic impairments induced by fenpropathrin via regulating oxidative stress, apoptotic, and inflammatory events in the brain of rats. Food Chem Toxicol 2020; (1):135.
Mohamed WA, Abd-Elhakim YM, Ismail SAA. Involvement of the anti-inflammatory, anti-apoptotic, and anti-secretory activity of bee venom in its therapeutic effects on acetylsalicylic acid-induced gastric ulceration in rats. Toxicology 2019; (5)1:419:11-23.
Ayurveda Aushadha sangrahaya, 2nd edition, volume I, part 2. Department of Ayurveda 1971; 58.
De Silva GN, Diddeniya JID, Dahanayake J, Perera PK. Traditional medicinal benefits of Caryota urens: A review article. Sri Lanka Journal of Indigenous Medicine (SLJIM) 2022 14:7(01):582–91.
Gousia Sk, Kumar Ka, Kumar TV, Latha Jn. Biological Activities and Medicinal Properties of Couroupita guianensis. International Journal of Pharmacy and Pharmaceutical Science Research 2013; 3(4): 140-143
Zein N, Mohamed EK, Elsayed FE. Anti-proliferative effect of Eucalyptus camaldulensis against ehrlich ascites carcinoma. World journal pharmaceutical research 2015; 4:272–286.
Srivastav AK, Singh R, Manimegalai S, Rajeswari VD. Identification of Flavonoids in Methanolic Extract of Caryota urens (Fish Tail Palm): A Phytochemical Screening Involving Structure Analysis by FTIR Spectroscopy. Research Journal of Phytochemistry. 2015 Sep 1;9(3):127–36.
Pinheiro MMG, Fernandes SBO, Fingolo CE, Boylan F, Fernandes PD. Anti-inflammatory activity of ethanol extract and fractions from Couroupita guianensis aublet leaves. Journal of Ethnopharmacology 2013; (3)7:146(1):324–30.
Saluja MS, Sangameswaran B, Sharma A, Dubey C. Phytochemical study and In-Vitro Cytotoxic Activity of Cinnamomum tammala linn. Against Ehrlich Ascites Carcinoma (EAC) and Dalton’s Ascitic Lymphoma (DAL) Cell Lines. Research J. Pharmacognosy and Phytochemistry 2010; 2(1): 37-40.
Khandelwal KR. Nirali prakashan Pune. 19th ed. Practical Pharmacognosy techniques and experiments 2008.
Saha P, Kumar DA, Kakoti BB, Selvan VT. Anticancer activity of methanol extract of Ipomoea reptans in EACb earing mice. Pharmacologyonline 2008; 2:808-820.
Tayel F, Mahfouz M E, Salama AF and Mansour MA. Ethoxyquin Inhibits the Progression of Murine Ehrlich Ascites Carcinoma through the Inhibition of Autophagy and LDH. Biomedicines 2021;9(11):1526.
Ahmed OM, Ahmed RR. Anti-proliferative and apoptotic efficacy of diallyl disulfide on Ehrlich ascites carcinoma. Hepatoma Research. 2015; 1: 67-74. http://dx.doi.org/10.4103/2394-5079.157602
Mishra S, Tamta AK, Sarikhani M, Desingu PA, Kizkekra SM, Pandit AS, et al. Subcutaneous Ehrlich Ascites Carcinoma mice model for studying cancer-induced cardiomyopathy. Scientific Reports. 2018; 4:8(1).
Abed El-Wahab Fouda FM. Histological and histochemical study on the effect of Ehrlich ascites carcinoma on the liver and kidney of mice and the possible protective role of tetrodotoxin. Egyptian Journal of Biology 2009; 11: 13-25.
Alkhatib MH, Nori DA, Al-Ghamdi MA. Cardiotoxicity and Hematoxicity of Sorafenib – Loaded Nanoemulsion in Ehrlich Ascites Carcinoma - Bearing Mice. Biosci Biotech Res Asia 2017;14(1):75-82.
Ali H, Hasi RY, Islam M, Haque MS, Alkhanani MF, Almalki AH, et al. Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet linn. in Ehrlich ascites carcinoma bearing Swiss albino mice. Scientific Reports 2022; (7)15:12(1).
Abd Eldaim MA, Tousson E, Soliman MM, El Sayed IET, Abdel Aleem AAH, Elsharkawy HN. Grape seed extract ameliorated Ehrlich solid tumor-induced hepatic tissue and DNA damage with reduction of PCNA and P53 protein expression in mice. Environmental Science and Pollution Research International. 2021; 28(32):44226–38.
Salem ML, Shoukry NM, Teleb WK, Abdel-Daim MM, Abdel-Rahman MA. In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model. Springerplus. 2016 May 10;5:570. doi: 10.1186/s40064-016-2269-3.
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