ANTI-METASTATIC POTENTIALITY OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME. AND O. RETICULATA BEDD. AGAINST SELECTED HUMAN CANCER CELL LINES
Abstract
To evaluate the anti-metastatic potentialities of purified anthocyanin from Osbeckia aspera (L.) Blume. and O. reticulata Bedd. against selected human cancer cell lines such as HT29 colon, MG63 bone and HeLa cervical by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, apoptosis and DNA fragmentation test. Anthocyanin was extracted from the in vitro callus culture of the Osbeckia species, purified using amberlite column chromatography and fractionated by LC-MS/MS. Anthocyanin producing callus cultures were trialed on MS medium fortified with various combinations of phytohormones and sucrose. Significant callus formation in O. aspera was initiated in cultures containing 0.5 mg/L of 2, 4-D and 0.5 mg/L BA, while that in O. reticulata was initiated with 1.2 mg/L BA and 1.4 mg/L NAA. The same hormonal combinations on sub-culturing turned white friable callus into red compact callus. Purified anthocyanins obtained from O. aspera and O. reticulata contained Malvidin-3 -diglucoside, delphinidin, cyanindin aglycone and Peonidin. Osbeckia species displayed differential responses against the HT29 colon, MG63 bone and HeLa cervical cancer cell lines in terms of IC50 values of toxicity. O. aspera was more effective against HeLa cervical cell lines (23.7µg/ml) followed by HT29 colon (64.7µg/ml) as compared to O. reticulata. Poor selectivity index was noticed with bone cancer cell lines. The results were substantiated by apoptotic analysis and DNA fragmentation results. The overall results suggest that the purified anthocyanin of O. aspera and O. reticulata was excellent as antimetastatic and warrant further studies to isolate novel compounds for chemotherapeutic use.
Keywords:
Osbeckia, anthocyanin, cytotoxic activity, human cancer cell lines, apoptosisDOI
https://doi.org/10.25004/IJPSDR.2018.100312References
2. Subramani R, Narayanasamy M, Feussner K-D. Plant-derived antimicrobials to fight against multi-drug-resistant human pathogens. Biotech. 2017; 7(3):172.
3. Wang L-S, Stoner GD. Anthocyanins and their role in cancer prevention. Cancer Lett. 2008; 269(2):281–90.
4. Sutharut J, Sudarat J. Total anthocyanin content and antioxidant activity of germinated colored rice. Int Food Res J. 2012; 19(1):215–21.
5. Kuete V, Karaosmanoğlu O, Sivas H. Anticancer Activities of African Medicinal Spices and Vegetables. In: Medicinal Spices and Vegetables from Africa. Elsevier; 2017. p. 271–97.
6. Devika V, Mohandass S. Apoptotic induction of crude extract of Foeniculum vulgare extracts on cervical cancer cell lines. IntJ Curr Microbiol App Sci. 2014; 3(3):657–61.
7. Paul S, Chakraborty S, Mukherjee A, Kundu R. Research Article Evaluation of Cytotoxicity and DNA Damaging Activity of Three Plant Extracts on Cervical Cancer Cell Lines. Int J Pharm Sci Rev Res. 2015; 31(37):183–9.
8. Cohen GM, Sun XM, Snowden RT, Dinsdale D, Skilleter DN. Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem J. 1992; 286(2):331–334.
9. Cohen GM, Sun XM, Fearnhead H, MacFarlane M, Brown DG, Snowden RT, et al. Formation of large molecular weight fragments of DNA is a key committed step of apoptosis in thymocytes. J Immunol. 1994; 153(2):507–516.
10. Hashemi SA, Abediankenari S, Ghasemi M, Azadbakht M, Yousefzadeh Y, Dehpour AA. The Effect of Fig Tree Latex (Ficus carica) on Stomach Cancer Line. Iran Red Crescent Med J. 2011; 13(4):272–275.
11. Sahranavard S, Naghibi F, Mosaddegh M, Esmaeili S, Sarkhail P, Taghvaei M, et al. Cytotoxic activities of selected medicinal plants from Iran and phytochemical evaluation of the most potent extract. Res Pharm Sci. 2009; 4(2):133–7.
12. Nemati F, Dehpouri AA, Eslami B, Mahdavi V, Mirzanejad S. Cytotoxic properties of some medicinal plant extracts from mazandaran, iran. Iran Red Crescent Med J. 2013; 15(11):e8871.
13. Tantengco OAG, Jacinto SD. Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines. Asian Pac J Trop Biomed. 2015; 5(12):1037–41.
14. Siegel R, Ma J, Zou Z, Jemal A. Cancer statistics, 2014. CA Cancer J Clin. 2014; 64(1):9–29.
15. Zulkipli IN, David SR, Rajabalaya R, Idris A. Medicinal Plants: A Potential Source of Compounds for Targeting Cell Division. Drug Target Insights. 2015; 9:DTI.S24946.
16. Canoy RJ, Lomanta J, M. Ballesteros P, Chun E, Dator R, D. Jacinto S. Cancer chemotherapeutic potential of endemic and indigenous plants of Kanawan, Morong, Bataan Province, Philippines. Vol. 20, Asia life sciences. 2011; 331-339.
17. Olarte EI, Herrera AA, Villaseñor IM, Jacinto SD. In vitro antitumor properties of an isolate from leaves of Cassia alata L. Asian Pac J Cancer Prev. 2013; 14(5):3191–3196.
18. Hsieh Y-J, Yang M-Y, Leu Y-L, Chen C, Wan C-F, Chang M-Y, et al. Kalanchoe tubiflora extract inhibits cell proliferation by affecting the mitotic apparatus. BMC Complement Altern Med. 2012; 12(1):1242.
19. Tantiado RG. Survey on ethnopharmacology of medicinal plants in Iloilo, Philippines. Int J Bio-Science Bio-Technology. 2012; 4(4):11–26.
20. Lirio SB, Macabeo APG, Paragas EM, Knorn M, Kohls P, Franzblau SG, et al. Antitubercular constituents from Premna odorata Blanco. J Ethnopharmacol. 2014; 154(2):471–4.
21. Abe R, Ohtani K. An ethnobotanical study of medicinal plants and traditional therapies on Batan Island, the Philippines. J Ethnopharmacol. 2013; 145(2):554–65.
Published
Abstract Display: 348 
PDF Downloads: 470 
