EFFECT OF ETHANOLIC EXTRACT OF FRUITS OF ERIOBOTRYA JAPONICA ON LIPID PROFILE AND BODY WEIGHT IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Abstract
Eriobotrya japonica locally called as loquat in Kashmir has been studied in various parts of the world but little work has been reported on Kashmiri loquat. The chemical nature of fruits and vegetables offers a great diversity of biological properties and plays an important role in the field of pharmacology. There is a quest for newer drugs with few adverse effects and this poses a challenge for the development of new drugs. The study was undertaken to study the activities of ethanolic extract of Eriobotrya japonica fruits in streptozotocin induced diabetic rats. The phytochemical screening of the plant was also done. The animals were divided into five groups. Normal Control group received only the vehicle. Toxic group included those animals in which diabetes was induced by streptozotocin. The 3rd group was those animals which received streptozotocin and standard antidiabetic drug-glibenclamide. 4th group included those diabetic animals which received 50 mg/kg b.w dose of fruits of Eriobotrya japonica. 5th group animals included those diabetic animals which received 100 mg/kg b.w of the plant extract. The biochemical parameters that were evaluated were blood glucose levels and lipid profile tests. The body weight was also checked. Histopathology of pancreas was also done. The results showed significant decrease in blood glucose levels, lipid profile tests in animals treated with different doses of the plant extracts. Histopathology of pancreas also showed positive results.
Keywords:
Eriobotrya japonica fruits, blood glucose levels, lipid profile, histopathologyDOI
https://doi.org/10.25004/IJPSDR.2019.110101References
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27. Varley H. Practical Clinical Biochemistry New Delhi CBS. Publishers and Distributors V edition 1980; 1: 457.
28. Trinder P. Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor. Ann Clin Biochem.1966; 6: 24-25
29. Trinder P. Glucose oxidase method. Ann Clin Biochem. 1969: 6.
30. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. CHOD-PAP method for determination of total cholesterol. Clin. Chem. 1974; 20: 470-475.
31. Friedewald WT, Levy RI, Frednickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in Plasma, without use of the preparative ultra-centrifuge. Clin Chem. 1972; 18: 499-502.
32. Izzo C, Grillo F, Muradcer E. Improved method for determination of high density lipoprotein cholesterol. Isolation of high-density lipoprotein by use of polyethylene glycol 6000. Clin. Chem. 1981; 27: 371-374.
33. Trivedi NA, Majumder B, Bhatt JD, Hemavathi KG. Effect of Shilajit on blood glucose and lipid profile in alloxan-induced diabetic rats. Ind J Pharmacol 2004; 36(6): 373-376.
34. Bancroft JD, Srevens A, Turner DR. Theory and practice of histological Techniques, 4th Edn (Churchill Livington, New York) 1996: 51.
35. Drury RAB, Wallington EA. Carletons histological technique, 6th edition, Oxford university press, London 1973: 124-136.
36. Michael JF. Diabetes Treatment, Part 1: Diet and Exercise. Clin Diabetes. 2007; 25(3): 105-109.
37. Pradeep R, Mohan V. The changing scenario of the diabetes epidemic: implications for India. Indian J Med Res. 2002; 116: 121-132.
2. King H. Diabetes mellitus: a growing international health care problem. Int Diab Monitor. 1997; 9: 1-6.
3. Grover JK, Yadav S, Vats V. Medicinal Plants of India with anti-diabetic potential. J Ethnopharmacol. 2002; 81(1): 81-100.
4. Rafiullah MRM, Siddiqui AW, Mir SR, Ali M, Pillai KK, Singh S. Antidiabetic Activity of some Indian Medicinal Plants. Pharmaceutical Biology 2006; 44(2): 95-99.
5. Rastogi RP, Mehrotra BN Compendium of Indian Medicinal Plants Vol V, New Delhi, India Publications and Information Directorate ( CSIR), 1995. p, 405
6. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants Vol. I, New Delhi, India, Publications and Information’s Directorate (CSIR), pp, 1999 326, 398.
7. Rastogi RP, Mehrotra BN. Compendium of India Medicinal Plants Vol II, New Delhi Publications and Information Directorate (CSIR), 1990 p, 398.
8. Rastogi RP, Mehrotra BN Compendium of Indian Medicinal Plants Vol II, New Delhi Publications and Information Directorate (CSIR), 1991. p, 660
9. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. CSIR Pub, New Delhi, 1966; 104.
10. Chopra RN, Chopra IC. A review of work on Indian medical plants, ICMR, New Delhi.1955.
11. Singh B, Gairola S, Kumar D, Gupta V, Bansal P. Pharmacological Potential of Eriobotrya japonica- An overview International Research Journal of Pharmacy. 2010;1(1): 95-99.
12. Chen J, Li WL, Wu JL, Ren BR, Zhang HQ. Hypoglycemic effects of a sequiterpene glycoside isolated from leaves of loquat (Eriobotrya japonica (Thumb,) Lindl. Phytomedicine. 2008; 15(1-2): 98-102.
13. El-Hossary GA, Fathy MM, Kassem HA et al. Phytochemical and biological investigations of Eriobotrya japonica growing in Egypt. Bulletin of Faculty of Pharmacy. 2000; 38:129-142
14. Huang Y, Li J, Cao Q, Yu SC, Lv XW, Jin Y et al. Anti-Oxidative effect of triterpene acids of Eriobotrya japonica (Thumb). Lindl. Leaf in chronic bronchitis rats. Life Science. 2006; 78: 2749-2757.
15. Ito H, Kobayashi E, Takamasto Y, Li SH, Hatano T, Sakagami H, et al. Polyphenols from Eriobotrya japonica and their cytotoxicity against human oral tumour cell lines. Chem Pharm Bull (Tokyo). 2000; 48(5): 687-693.
16. Yokoto J, Takuma D, Hamada A, Onogawa M, Yoshioka S, Kusenose M et al. Scavenging of reactive oxygen species by Eriobotrya Japonica seed extract. Biological and Pharmaceutical Bulletin, 2006; 29: 467-471.
17. Hamada A, Yoshioka S, Takuma D, Yokota J, Cui T, Kusunose M, et al. The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats. Biol and Pharm Bull. 2004; 27: 1961-1964.
18. Know HK, Kang MJ, Kim HJ, Choi JS, Paik KJ, Chung HY. Inhibition of NF kappa B by methyl chlorogenate from Eriobotrya japonica. Molecules and Cells. 2000; 10: 241-246.
19. Nishioka Y, Yoshioka S, Kusunose M, Cui T, Hamada A, Ono M et al. Effects of extracts derived from Eriobotrya japonica on liver function improvement in rat. Biol and Pharm Bull. 2002 ; 25: 1053-1057.
20. Shimizu M, Fukumura H, Tsuji H, Tanaami S, Hayashi T, Morita N. Anti-inflammatory constituents of topically applied crude drugs constituents and anti-inflammatory effect of Eriobotrya japonica Lindl. Chem and Pharm Bull. 1986; 34: 2614-2617.
21. Ju JH, Zhou L, Lin G, Liu D, Wang LW, Yang JS. Studies on constituents of triterpene acids from Eriobotrya Japonica and their anti-inflammatory and anti-tussive effects. J of Chinese Pharmacol. 2003; 38: 752-757.
22. Banno N, Akihisa T, Tokuda H, Yasukawa K, Taguchi Y, Akazawa H et al. Anti inflammatory and antitumor-promoting effects of the triterpene acids from the leaves of Eriobotrya japonica. Biol and Pharm Bull. 2005; 28: 1995-1999.
23. Harborne JB. Phytochemical methods, Chapman and Hall Ltd., London, 1973. pp. 49-188.
24. Rasool R, Ganai BA, Akbar S, Kamili AN, Masood A. Phyto chemical screening of Prunella vulgaris L. An important Medicinal Plant of Kashmir Pak. J. Pharm Sci., 2010; 23(.4): 399-402.
25. Trease GE, Evans WC. Pharmacognosy, 11th edn., Brailliar Tiridel Can., Macmillian Publishers1989.
26. Babu V, Gangadevi T, Subramanian A. Antidiabetic activity of ethanol extract of Cassia Klenii leaf in streptozotocin-induced diabetic rats and isolation of an active fraction and toxicity evaluation of the extract. Ind J of Pharmacol. 2003; 35: 290-296.
27. Varley H. Practical Clinical Biochemistry New Delhi CBS. Publishers and Distributors V edition 1980; 1: 457.
28. Trinder P. Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor. Ann Clin Biochem.1966; 6: 24-25
29. Trinder P. Glucose oxidase method. Ann Clin Biochem. 1969: 6.
30. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. CHOD-PAP method for determination of total cholesterol. Clin. Chem. 1974; 20: 470-475.
31. Friedewald WT, Levy RI, Frednickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in Plasma, without use of the preparative ultra-centrifuge. Clin Chem. 1972; 18: 499-502.
32. Izzo C, Grillo F, Muradcer E. Improved method for determination of high density lipoprotein cholesterol. Isolation of high-density lipoprotein by use of polyethylene glycol 6000. Clin. Chem. 1981; 27: 371-374.
33. Trivedi NA, Majumder B, Bhatt JD, Hemavathi KG. Effect of Shilajit on blood glucose and lipid profile in alloxan-induced diabetic rats. Ind J Pharmacol 2004; 36(6): 373-376.
34. Bancroft JD, Srevens A, Turner DR. Theory and practice of histological Techniques, 4th Edn (Churchill Livington, New York) 1996: 51.
35. Drury RAB, Wallington EA. Carletons histological technique, 6th edition, Oxford university press, London 1973: 124-136.
36. Michael JF. Diabetes Treatment, Part 1: Diet and Exercise. Clin Diabetes. 2007; 25(3): 105-109.
37. Pradeep R, Mohan V. The changing scenario of the diabetes epidemic: implications for India. Indian J Med Res. 2002; 116: 121-132.
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20-01-2019
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“EFFECT OF ETHANOLIC EXTRACT OF FRUITS OF ERIOBOTRYA JAPONICA ON LIPID PROFILE AND BODY WEIGHT IN STREPTOZOTOCIN INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 11, no. 1, Jan. 2019, pp. 1-6, https://doi.org/10.25004/IJPSDR.2019.110101.
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How to Cite
“EFFECT OF ETHANOLIC EXTRACT OF FRUITS OF ERIOBOTRYA JAPONICA ON LIPID PROFILE AND BODY WEIGHT IN STREPTOZOTOCIN INDUCED DIABETIC RATS”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 11, no. 1, Jan. 2019, pp. 1-6, https://doi.org/10.25004/IJPSDR.2019.110101.
