Anti-Inflammatory Effects of Vitex altissima Leaf Extract in Lipopolysaccharide-Induced RAW 264.7 Macrophages.
Abstract
Vitex altissima has been conventionally utilized for its wide-ranging properties in the management of oxidative stress and inflammation. The present investigation was centered on the quantification of the anti-inflammatory efficacy of Vitex altissima. The samples were evaluated for their ability to impede the activity of proteinase, denature proteins, and stabilize the membrane of human red blood cells (HRBC). The present study investigated the inhibitory impact of extracts on the production of total cyclooxygenase, lipoxygenase-5 (5-LOX), myeloperoxidase (MPO), and nitric oxide (NO) using the RAW 264.7 cell line. Furthermore, the antioxidant properties were assessed through the employment of both DPPH assay and reducing power assay. According to the findings, the methanolic extract of Vitex altissima (VAME) was identified as the most efficacious fraction with anti-inflammatory and antioxidant properties. The findings indicate that the extracts exhibited dose-dependent inhibition of proteinase, protein denaturation, and hemolysis of HRBC membrane, which is beneficial. The extracts of Vitex altissima, when treated at concentrations that are not cytotoxic, were observed to have a significant effect in reducing the activity of COX, 5-LOX, and MPO in RAW 264.7 cell line treated with LPS. This resulted in a decrease in NO levels. The dose-dependent increase in in vitro anti-inflammatory activity of Vitex altissima suggests its potential use as a pharmacological agent for the management of diseases related to inflammation. Additional comprehensive phytochemical investigations, in conjunction with in vitro and in vivo analyses, are necessary to identify the active constituent within the extract.
Keywords:
Vitex altissima, antioxidant, inflammation, macrophages, LPS, Nitrite.DOI
https://doi.org/10.25004/IJPSDR.2023.150410References
Yatoo M, Gopalakrishnan A, Saxena A, Parray OR, Tufani NA, Chakraborty S, Tiwari R, Dhama K, Iqbal H. Anti-inflammatory drugs and herbs with special emphasis on herbal medicines for countering inflammatory diseases and disorders-a review. Recent Adv Inflamm Allergy Drug Discov. 2018 May 1;12(1):39-58. https://doi.org/10.2174/1872213x12666180115153635
Shingnaisui K, Dey T, Manna P, Kalita J. Therapeutic potentials of Houttuynia cordata Thunb. against inflammation and oxidative stress: A review. J Ethnopharmacol. 2018 Jun 28;220:35-43. https://doi.org/10.1016/j.jep.2018.03.038
Ayyanar M, Ignacimuthu S. Medicinal plants used by the tribals of Tirunelveli hills, Tamil Nadu to treat poisonous bites and skin diseases. Indian J. Tradit. Know. 2005; 4, 229–236.
Narayana Rao K, Thammanna T. Medicinal Plants of Tirumala. T.T.D, Tirupati, India. 1990: 57.
Sridhar C, Rao KV, Subbaraju GV. Flavonoids, triterpenoids and a lignan from Vitex altissima. Phytochemistry. 2005 Jul 1;66(14):1707-12. https://doi.org/10.1016/j.phytochem.2005.05.008
Ravipati AS, Zhang L, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, Smith PT, Shanmugam K, Münch G, Wu MJ, Satyanarayanan M. Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complement Altern Med. 2012 Dec;12(1):1-4. https://doi.org/10.1186/1472-6882-12-173
Shim JU, Oh PS, Lim KT. Anti-inflammatory activity of ethanol extract from Geranium sibiricum Linne. J Ethnopharmacol. 2009 Oct 29;126(1):90-5. https://doi.org/10.1016/j.jep.2009.08.004
Abd‐El‐Aleem SA, Ferguson MW, Appleton I, Bhowmick A, McCollum CN, Ireland GW. Expression of cyclooxygenase isoforms in normal human skin and chronic venous ulcers. J Pathol. 2001 Dec;195(5):616-23. https://doi.org/10.1002/path.992.
Bruch-Gerharz D, Fehsel K, Suschek C, Michel G, Ruzicka T, Kolb-Bachofen V. A proinflammatory activity of interleukin 8 in human skin: expression of the inducible nitric oxide synthase in psoriatic lesions and cultured keratinocytes. J Exp Med. 1996 Nov 1;184(5):2007-12. https://doi.org/10.1084/jem.184.5.2007
Hwang JH, Ma JN, Park JH, Jung HW, Park YK. Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages. Int. J. Mol. Med. 2019 Jan 1;43(1):26-36. https://doi.org/10.3892/ijmm.2018.3937
Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem. 2001 Jul 16;49(7):3420-4. https://doi.org/10.1021/jf0100907
Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. Diet. 1986;44(6):307-15.
Djuichou Nguemnang SF, Tsafack EG, Mbiantcha M, Gilbert A, Atsamo AD, Yousseu Nana W, Matah Marthe Mba V, Adjouzem CF. In vitro anti-inflammatory and in vivo antiarthritic activities of aqueous and ethanolic extracts of Dissotis thollonii Cogn.(Melastomataceae) in rats. Evid Based Complement Alternat Med. 2019 Nov 15;2019. https://doi.org/10.1155/2019/3612481
Tantary S, Masood A, Bhat AH, Dar KB, Zargar MA, Ganie SA. In vitro antioxidant and RBC membrane stabilization activity of Euphorbia wallichii. Free Radicals and Antioxidants. 2017;7(1):13-22. https://doi.org/10.5530/fra.2017.1.3
Gunathilake KD, Ranaweera KK, Rupasinghe HV. In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines. 2018 Nov 19;6(4):107. https://doi.org/10.3390/biomedicines6040107
Talarico LB, Zibetti RG, Faria PC, Scolaro LA, Duarte ME, Noseda MD, Pujol CA, Damonte EB. Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata. Int J Biol Macromol. 2004 Apr 1;34(1-2):63-71. https://doi.org/10.1016/j.ijbiomac.2004.03.002
Walker MC, Gierse JK. In vitro assays for cyclooxygenase activity and inhibitor characterization. Cyclooxygenases: Methods and Protocols. 2010:131-44. https://doi.org/10.1007/978-1-59745-364-6_11
Axelrod B. Lipoxygenase from soybeans. Methods enzymol. 1981;71:441-51. https://doi.org/10.1016/0076-6879(81)71055-3
Suzuki K. Ota H, Sasagawa S, Sakatani T, Fujikura T. Assay method for myeloperoxidase in human polymorphonuclear leukocytes. Anal Biochem. 1983;132:345-52. https://doi.org/10.1016/0003-2697(83)90019-2
Lepoivre M, Chenais B, Yapo A, Lemaire G, Thelander L, Tenu JP. Alterations of ribonucleotide reductase activity following induction of the nitrite-generating pathway in adenocarcinoma cells. J. Biol. Chem. 1990 Aug 25;265(24):14143-9. https://doi.org/10.1016/S0021-9258(18)77279-7
Sangeetha G, Vidhya R. In vitro anti-inflammatory activity of different parts of Pedalium murex (L.). inflammation. Int. J. Herb. Med. 2016;4(3):31-6.
Tosun M, Ercisli S, Sengul M, Ozer H, Polat T, Ozturk E. Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biol Res. 2009;42(2):175-81. http://dx.doi.org/10.4067/S0716-7602009000200005
Govindappa M, Naga SS, Poojashri MN, Sadananda TS, Chandrappa CP. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L.) Hitchc. J. Pharmacogn. Phytotherapy. 2011 Apr;3(3):43-51.
Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in–vitro antioxidant and anti–inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed 2013 Aug 1;3(8):623-7. https://doi.org/10.1016/S2221-1691(13)60126-4
Gunathilake KD, Ranaweera KK, Rupasinghe HV. In vitro anti-inflammatory properties of selected green leafy vegetables. Biomedicines. 2018 Nov 19;6(4):107. https://doi.org/10.3390/biomedicines6040107
Augusto O, Kunze KL, de Montellano PO. N-Phenylprotoporphyrin IX formation in the hemoglobin-phenylhydrazine reaction. Evidence for a protein-stabilized iron-phenyl intermediate. J. Biol. Chem. 1982 Jun 10;257(11):6231-41. https://doi.org/10.1016/S0021-9258(20)65129-8
Oyeleke SA, Ajayi AM, Umukoro S, Aderibigbe AO, Ademowo OG. Anti-inflammatory activity of Theobroma cacao L. stem bark ethanol extract and its fractions in experimental models.J Ethnopharmacol. 2018 Aug 10; 222:239-48. https://doi.org/10.1016/j.jep.2018.04.050
Trouillas P, Calliste CA, Allais DP, Simon A, Marfak A, Delage C, Duroux JL. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas. Food Chem. 2003 Mar 1;80(3):399-407. https://doi.org/10.1016/S0308-8146(02)00282-0
Pulli B, Ali M, Forghani R, Schob S, Hsieh KL, Wojtkiewicz G, Linnoila JJ, Chen JW. Measuring myeloperoxidase activity in biological samples. PloS one. 2013 Jul 5;8(7): e67976. https://doi.org/10.1371/journal.pone.0067976
Kubes P, McCafferty DM. Nitric oxide and intestinal inflammation. Am J Med. 2000 Aug 1;109(2):150-8. https://doi.org/10.1016/S0002-9343(00)00480-0
Ravipati AS, Zhang L, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, Smith PT, Shanmugam K, Münch G, Wu MJ, Satyanarayanan M. Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complement Altern Med. 2012 Dec;12(1):1-4. https://doi.org/10.1186/1472-6882-12-173
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