Nephroprotective effect of the methanolic fruit extract of Musa balbisiana Colla against carbon tetrachloride-induced toxicity in Swiss albino mice
Abstract
Renal failure is the loss of renal functions owing to multiple factors, including oxidative stress, and it is a global concern, as it costs millions of lives every year. Carbon tetrachloride is a standard toxicant exploited to induce nephrotoxicity in experimental animals to study oxidative stress-related renal injury. This study was conducted to determine the nephroprotective activity of a methanolic extract derived from the unripe fruit pulp of Musa balbisiana Colla (MBME) in carbon tetrachloride-induced toxicity in Swiss albino mice. Multiple groups of mice were treated with a single dose of carbon tetrachloride (1-mg/kg body weight) intraperitoneally once in a week followed by oral administration of the methanolic fruit extract of M. balbisiana Colla (MBME) at two different doses (200 and 400 mg/kg body weight) consecutively for 28 days. Treatment with carbon tetrachloride reduced body weight along with enzymatic and non-enzymatic antioxidants in the renal tissue, elevated kidney weight, renal biomarkers, lipid peroxidation, IL-6, TNF-α, and TGF-β levels in the blood serum, impaired antioxidant system and up-regulated TGF-β expression in the kidney tissue, and brought histological changes in the architecture of renal tissues. Our data revealed that the oral administration of the methanolic fruit extract of M. balbisiana Colla (MBME) dose wisely restored kidney and body weight, renal biomarkers, enzymatic and non-enzymatic antioxidants in the renal tissue, reduced lipid peroxidation, interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-β (TGF-β) levels in the blood serum, re-established antioxidant system and down-regulated TGF-β expression in the kidney tissue, and re-established the architecture of renal tissues. Thus, this study reported the nephroprotective effects of methanolic fruit extract of M. balbisiana Colla (MBME) in carbon tetrachloride-induced toxicity in Swiss albino mice through possible antioxidant and anti-inflammatory activity.
Keywords:
antioxidant enzymes, carbon tetrachloride, histopathological, immunohistochemistry, nephrotoxicityDOI
https://doi.org/10.25004/IJPSDR.2024.160104References
Marques TR, Caetano AA, Henrique S, Cesar P, Braga MA, Henrique A, Machado, Raimundo VS, Angelita DC. Antioxidant activity and hepatoprotective potential of lyophilized extract of Acerola bagasse against CCl4‐induced hepatotoxicity in Wistar rats. J Food Biochem. 2018;42(6):e12670. https://doi.org/10.1111/jfbc.12670
Murray IV, Paolini MA. Histology, Kidney and Glomerulus. In: StatPearls. Treasure Island (FL): StatPearls Publishing, 2023. https://www.ncbi.nlm.nih.gov/books/NBK554544/
Howell HR, Brundige ML, Langworthy L. Drug Induced Acute Renal Failure. US Pharm. 2007;32(3):45-50. https://www.uspharmacist.com/article/drug-induced-acute-renal-failure
GhaneShahrbaf F, Assadi F. Drug-induced renal disorders. J Renal Inj Prev. 2015;4(3): 57-60. doi: 10.12861/jrip.2015.12
Crews DC, Bello AK, Saadi G. 2019 World Kidney Day Editorial - burden, access, and disparities in kidney disease. J Bras Nefrol. 2019;41(1), 1–9. doi: 10.1590/2175-8239-JBN-2018-0224
Makni M, Chtourou Y, Garoui E, Boudawara T. Carbon tetrachloride-induced nephrotoxicity and DNA damage in rats: protective role of vanillin. Hum & Exp Toxicol. 2012;31(8), 844–852. doi: 10.1177/0960327111429140
Bhattacharjya DK, Kar A, Sarma H, Patowari N. Notes on herbal treatments practiced by the people of Fringe village of Manas National Park, India, Indian J Tradit Knowle. 2015;1(1) 155-160
Kusuma SAF, Mita SR, Firdayani I, Mustarichie R. Study On The Antibacterial Activity Of Fruit Extracts Of Klutuk Banana (Musa Balbisiana Colla) Against Shigella dysenteriae Atcc 13313, Asian J Pharm Clin Res. 2017;10(7), 220-223
Jalani FF, Mohamad S, Shahidan WNS. Antibacterial effects of banana pulp extracts based on different extraction methods against selected microorganisms, Asian J Biomed Pharm Sci. 2014; 4(36), 14-19
Zubair NV, Suresh A, Babu G, Asma MA, Prahlad A, Hanisha KP. Assessment of anti-ulcer potential of unripe fruit extract of Musa balbisiana in stress induced ulcer model. World J Pharm Pharm Sci. 2018;7(8):1328-36
Kumari, S, Katare PB, Elancheran R, Nizami HL, Paramesha B, Arava S, Sarma PP, Kumar R, Mahajan D, Kumar Y, Devi R, Banerjee SK. Musa balbisiana Fruit Rich in Polyphenols Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats via Inhibition of Inflammation and Oxidative Stress. Oxidative med cellular longev. 2020; 2020, 7147498. https://doi.org/10.1155/2020/7147498
Irawan C, Utami A, Styani E, Putri ID, Putri RK, Dewanta A, Ramadhanti A. Potential of ethanolic extract from Ripe Musa balbisiana colla fruit using ultrasound-assisted extraction as an antioxidant and anti-gout. Pharmacogn J. 2021;13(6), 1332–1340. https://doi.org/10.5530/pj.2021.13.168
Nhon Hoang TN, Phan TT, Lien Phan TK, Van Nguyen NH, Dao Dong TA, Anh Le TH. Phytochemical screening, extraction, and determination of the bioactivities of the extract-enriched polyphenols and saponins from Musa Balbisiana Fruit. J Food Processing Preserv. 2023;2023, 1–16. https://doi.org/10.1155/2023/2581641
Li L, Zhou YF, Li YL, Wang LL, Arai H, Xu Y. In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni. World J Gastroenterol. 2017;23(16): 2912-2927. doi: 10.3748/wjg.v23.i16.2912
Dharajiya D, Patel P, Patel M, Moitra N. In vitro antimicrobial activity and qualitative phytochemical analysis of Withania somnifera (L.) Dunal extracts. Int J Pharm Sci Rev Res. 2014;27(2): 349-354. https://globalresearchonline.net/journalcontents/v27-2/60.pdf
Bellassoued K, Ben Hsouna A, Athmouni K, van Pelt J, Makni Ayadi F, Rebai T, Elfeki A. Protective effects of Mentha piperita L. Leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats. Lipids Health Dis. 2018;17(1). doi: 10.1186/s12944-017-0645-9
Bruckner J.V, MacKenzie W.F, Muralidhara S, Luthra R, Kyle M, Acosta D. Oral toxicity of carbon tetrachloride: acute, subacute and subchronic studies in rats. Fundam Appl Toxicol. 1986;6: 16–34. doi: 10.1016/0272-0590(86)90260-5
Ebaid H, Al-Tamimi J, Habila M, Hassan I, Rady A, Alhazza IM. Potential therapeutic effect of synthesized AgNP using curcumin extract on CCl4-induced nephrotoxicity in male mice. J. King Saud Univ. Scien. 2021;33(2):101356. https://doi.org/10.1016/j.jksus.2021.101356
Kieswich JE, Chen J, Alliouachene S, Caton PW, McCafferty K. Thiemermann C. Yaqoob MM. Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome. Bio Protoc. 2020;10(18):e3751. doi: 10.21769/BioProtoc.3751
Mohammadi S, Karimi J, Tavilani H, Khodadadi I, Mohseni R, Hashemnia M. Resveratrol downregulates TGF-β1 and Smad3 expression and attenuates oxidative stress in CCl4-induced kidney damage in rats. Asian Pac J Trop Biomed. 2020;10(9): 397-402. doi:10.4103/2221-1691.290130
Safhi MM. Nephroprotective Effect of Zingerone against CCl4-Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism. Oxidative Med Cell Longev. 2018;2018:1-7. https://doi.org/10.1155/2018/2474831
Emam N, Anjum S, Okail H, Ibrahim M, Ahmad T. Pomegranate peel extract protects against carbon tetrachloride induced nephrotoxicity in mice through increasing antioxidants status. Biomed Rep. 2020;13(3):13. doi: 10.3892/br.2020.1320
Rezagholizadeh L, Ojarudi M, Moradi A, Salimnejad R, Khonakdar-Tarsi A, Matin S, Feizi I, Mohammadnia A, Mazani M. Protective effects of Cinnamomum zeylanicum and Zingiber officinale extract against CCl4-induced acute kidney injury in rats. Physiol Pharmacol. 2022;26(2):158-167. doi:http://dx.doi.org/10.52547/phypha.26.2.6
Shahat AA, Ullah R, Alqahtani AS, Hassanein HM, Husseiny HA, Mohammed NM, Herqash RN. Nephroprotective effect of persimmon leaves (Diospyros kaki L.f.) against CCl4-induced renal toxicity in Swiss Albino rats. Drug Chem Toxicol. 2022;45:4, 1578-1586. doi: 10.1080/01480545.2020.1849269
Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh M, Zangeneh A. Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination. Arch Biol Sci. 2017;69(3):535-43. https://doi.org/10.2298/ABS160914129S
Lin Y, Zhen Y, Wei J, Wei J, Dai J, Gao J, Hu G. Rhein lysinate protects renal function in diabetic nephropathy of KK/HlJ mice. Exp Ther Med. 2017;14, 5801-5808. doi: 10.3892/etm.2017.5283
Seki M, Nakayama M, Sakoh T, Yoshitomi R, Fukui A, Katafuchi E, Tsuda S, Nakano T, Tsuruya K, Kitazono T. Blood urea nitrogen is independently associated with renal outcomes in Japanese patients with stage 3-5 chronic kidney disease: a prospective observational study. BMC Nephrol. 2019;20(1):115. doi: 10.1186/s12882-019-1306-1
Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AA, Vernekar SN. Markers of renal function tests. North American J med sci. 2010; 2(4), 170–173
Gounden V, Bhatt H, Jialal I. Renal Function Tests. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2023 https://www.ncbi.nlm.nih.gov/books/NBK507821/
Srivastava A, Kaze AD, McMullan CJ, Isakova T, Waikar SS. Uric Acid and the Risks of Kidney Failure and Death in Individuals With CKD. American J kidney dis. 2018;71(3), 362–370. https://doi.org/10.1053/j.ajkd.2017.08.017
Gyurászová M, Gurecká R, Bábíčková J, Tóthová Ľ. Oxidative Stress in the Pathophysiology of Kidney Disease: Implications for Noninvasive Monitoring and Identification of Biomarkers. Oxid Med Cell Longev. 2020;2020:5478708. doi: 10.1155/2020/5478708
Yilmaz-Ozden T, Can A, Karatug A, Pala-Kara Z, Okyar A, Bolkent S. Carbon tetrachloride-induced kidney damage and protective effect of Amaranthus lividus L. in rats. Toxicol Ind Health. 2016;32(6):1143-52. doi: 10.1177/0748233714555390
Venkatanarayana G, Sudhakara G, Sivajyothi P, Indira P. Protective effects of curcumin and vitamin E on carbon tetrachloride-induced nephrotoxicity in rats. EXCLI J. 2012;11:641-650.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099890/
Elsawy H, Badr GM, Sedky A, Abdallah BM, Alzahrani AM, Abdel-Moneim AM. Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats. PeerJ. 2019;7:e7011. doi: 10.7717/peerj.7011
Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol. 2003;33(2):105-36. doi: 10.1080/713611034
Su H, Lei CT, Zhang C. Interleukin-6 Signaling Pathway and Its Role in Kidney Disease: An Update. Front Immunol. 2017;8:405. doi: 10.3389/fimmu.2017.00405
Zhang T, Xiang L. Honokiol alleviates sepsis-induced acute kidney injury in mice by targeting the miR-218-5p/heme oxygenase-1 signaling pathway. Cell Amp Mol Biol Lett. 2019;24(1). doi: 10.1186/s11658-019-0142-4
Alum EU, Famurewa AC, Orji OU, Aja PM, Nwite F, Ohuche SE, Ukasoanya SC, Nnaji LO, Joshua D, Igwe KU, Chima SF. Nephroprotective effects of Datura stramonium leaves against methotrexate nephrotoxicity via attenuation of oxidative stress-mediated inflammation and apoptosis in rats. Avicenna J Phytomed. 2023;13(4):377-387. doi: 10.22038/AJP.2023.21903
Chung S, Overstreet JM, Li Y, Wang Y, Niu A, Wang S, Fan X, Sasaki K, Jin GN, Khodo SN, Gewin L, Zhang MZ, Harris RC. TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration. JCI Insight. 2018;3(21):e123563. doi: 10.1172/jci.insight.123563
Al-Medhtiy MH, Jabbar AA, Shareef SH, Ibrahim IAA, Alzahrani AR, Abdulla MA. Histopathological Evaluation of Annona muricata in TAA-Induced Liver Injury in Rats. Processes. 2022;10(8):1613. https://doi.org/10.3390/pr10081613
Li Z, Zhang W. Protective effect of berberine on renal fibrosis caused by diabetic nephropathy. Mol Med Rep. 2017;16(2):1055-62. doi: 10.3892/mmr.2017.6707
Evrard SM, d'Audigier C, Mauge L, Israël-Biet D, Guerin CL, Bieche I, Kovacic JC, Fischer AM, Gaussem P, Smadja DM. The profibrotic cytokine transforming growth factor-β1 increases endothelial progenitor cell angiogenic properties. J Thromb Haemost. 2012;10(4):670-9. doi: 10.1111/j.1538-7836.2012.04644.x
Published
Abstract Display: 443 
PDF Downloads: 400 
