Effect of Synthetic Red Dye Orange Red and Natural Red Dye Alizarin on Biochemical and Hematological Parameters in Male Wistar Rats
Abstract
Nowadays synthetic food dyes are mostly preferred than natural plant derived dyes due to low cost and intense coloration. In this study hematological and biochemical parameters were determined in male wistar rats after 30 days treatment with synthetic red dye orange red and natural plant derived red dye alizarin. 25 male wistar rats were divided into 5 groups with 5 animals per group. Group I rats were taken as control treated with normal rat diet and distilled water. Group II and III rats (experimental) were oral gavaged with 50 mg and 150 mg/kg body weight of alizarin dye. Group IV and V rats (experimental) were gavaged with 50 mg and 150 mg/kg body weight of orange red dye. Treatment of group V rats with 150 mg/kg body weight of orange red dye produce significant changes in RBC, Hb, Hct, MCH, serum aminotransferase enzymes and serum protein fraction. In comparison to this in group IV rats a significant change was observed only in Hb, serum aminotransferase enzymes and serum protein fraction when compared with control (group I) rats. However in group II and III alizarin treated rats no significant change was observed in different biochemical and hematological parameters relative to their respective control. In conclusion synthetic orange red dye proved to be more toxic than natural plant derived red dye alizarin.
Keywords:
Orange red dye, alizarin, hematological and biochemical parameters, wistar rats.DOI
https://doi.org/10.25004/IJPSDR.2019.110511References
Sharma S, Goyal RP, Chakravarty G, Sharma A. Toxicity of tomato red, a popular food dye blend on male albino mice. Experimental and Toxicologic Pathology. 2008;60(1):51-7.
Anna H, Christian RV. The potential use of organically grown dye plants in the organic textile industry. Experiences and results on the cultivation and yields of dyers chamomile (Anhemis tinctoria L.), Dyers Knotweed (Polygonum tinctoria) and Weld (Reseda luteola L.). Journal of Sustainable Agriculture. 2003;23(2):17–40.
Hussein SA, Barakat HH, Merfort I, Nawwar MA. Tannins from the leaves of Punica granatum. Phytochemistry. 1997;45(4):819-23.
Macioszek VK, Kononowicz AK. The evaluation of the genotoxicity of two commonly used food colors: Quinoline Yellow (E 104) and Brilliant Black BN (E 151). Cellular and Molecular Biology Letters. 2004;9(1):107-22.
Anliker R. Ecotoxicology of dyestuffs a joint effort by industry. Ecotoxicology and environmental safety. 1979;3(1):59-74.
Turkoglu S. Genotoxicity of five food preservatives tested on root tips of Allium cepa. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2007;626(1-2):4-14.
Tsuda S, Murakami M, Matsusaka N, Kano K, Taniguchi K, Sasaki YF. DNA damage induced by red food dyes orally administered to pregnant and male mice. Toxicology Science. 2001;61(1):92-99.
Montaser MM, Alkafafy ME. Effects of Synthetic Food Colour (Carmoisine) on Expression of Some Fuel Metabolism Genes in Liver of Male Albino Rats. Life Science Journal 2013;10(2): 2191-98.
Ali MO, Sharaf AK, El-Deen OM, El-Menshay OM, Bakery S. Genotoxic effects of fluoxetine on the chromosomes of bone marrow cells of pregnant mice. 39th Annual International Meeting, The International Association of Forensic Toxicologists TIAFT. 200, 2001, 27.
Tema Nord. Food additives in Europe 2000; Status of safety assessments of food additives presently permitted in the EU, Tema Nord. 2002;560:116-121.
Mathur NRA, Chaudhary V, Mehta M, Krishnatrey R. Effect of Sunset Yellow on testis in rats. Journal of Ecophysiology and Occupational Health. 2005;5(1):1-3.
Poul M, Jarry G, Elhkim MO, Poul JM. Lack of genotoxic effect of food dyes amaranth, Sunset Yellow and Tartrazine and their metabolites in the gut micronucleus assay in mice. Food and Chemical Toxicology. 2009;47(2):443-448.
Westmoreland C, Gatehouse DG. The differential clastogenicity of Solvent Yellow 14 and FD & C Yellow No. 6 in vivo in the rodent micronucleus test (observations on species and tissue specificity). Carcinogenesis. 1991;12(8):1403-1407.
Orban N, Boldizsar I, Szucs Z, Danos B. Influence of different elicitors on the synthesis of anthraquinone derivatives in Rubia tinctorum L. cell suspension cultures. Dyes and Pigments. 2008;77(1):249-257.
Ozen E, Yeniocak M, Goktas O, Alma MH, Yilmaz F. Antimicrobial and antifungal properties of madder root (Rubia tinctorum) colorant used as an environmentally-friendly wood preservative. Bioresources. 2014;9(2):1998-2009.
Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology. 1957;28(1):56-63.
Belfield A, Goldberg D. Colorimetric determination of alkaline phosphatase activity. Enzyme. 1971;12(5):561-568.
Doumas BT. Standards for total serum protein assay-a collaborative study. Clinical Chemistry. 1975;21(8):1159-1166.
Lanter AL. Clinical Biochemistry W. B. Saunders Company, Philadelphia, 7 th edition, 1975, 147-159.
Rodak LC. Routine testing in haematology. In: Diagnostic haematology. W.B. London, Toronto. 1995, PP. 128-144.
Jain NC, Schalm’s Veterinary Heamatology, 4th Ed., Lea and Fabiger, Philadelphia, 1986.
Macioszek VK, Kononowicz AK. The evaluation of the genotoxicity of two commonly used food colours; Quinoline Yellow (E104) and Brilliant Black BN (E151). Cellular and Molecular Biology Letters. 2004;9(1):107-122.
Shaker AMH, Ismail IA, El-Nemr SE. Effect of different food stuff colorants added to casein diet on biological evaluation. Bull. Nutr. Inst. Cairo. 1989;9(1):77-86.
Tsuda S, Murakami M, Matsusaka N, Kano K, Taniguchi K, Sasaki YF. DNA damage induced by red food dyes orally administered to pregnant and male mice. Toxicology Science. 2001;61(1):92- 99.
Babu S, Shenolikar IS. Health and nutritional implications of food colors. Indian Journal of Medical Research. 1995;102:245-249.
Yuan G, Dai S, Yin Z, Lu H, Jia R, Xu J, Zhao X. Toxicological assessment of combined lead and cadmium: Acute and sub-chronic toxicity study in rats. Food and Chemical Toxicology. 2014;65:260-268.
Alsolami MA. Effect of a food additive on certain haematological and biochemical parameters in male albino rat. International Journal of Zoology and Research. 2017;7:1-10.
Ates B, Orun I, Talas ZS, Durmaz G, Yilmaz I. Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792)exposed to Pb2+ and Cu2+. Fish Physiology and Biochemistry. 2008;34(1):53-59.
Govindwar SP, Dalvi RR. Age dependent Toxicity of a corn extract in young and old male rats. Veterinary and Human Toxicology. 1990;32(1):23-26.
Mekkawy HA, Ali MO, El-Zawahry AM. Toxic effect of syntheticand natural food dyes on renal and hepatic functions in rats. Toxicology Letters. 1998;95(1001):155-155.
Aboel-Zahab H, El-Khyat Z, Sidhom G, Awadallah R, Abdel–Al W, Mahdy K. Physiological effects of some synthetic food colouring additives on rats. Bollettino Chimico Farmaceutico. 1997;136(10):615 – 627.
Amin KA, Hameid HA, Elsttar AA. Effect of food azo dyes tartrazine and Carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food and Chemical Toxicology. 2010;48(10):2994-2999.
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