A Fission Yeast Bioassay for Rapid Screening for Antiproliferative Agents from Bauhinia variegata and Leptadenia reticulata
Abstract
The aim of the present study was to evaluate the antiproliferative activity of crude extracts from leaves of Bauhinia variegata and Leptadenia reticulata using fission yeast Schizosaccharomyces pombe as a model. The crude extracts of both plants were prepared by three experimental conditions (25°C, elevated temperature and ultrasound waves) and four solvent systems [Water, Methanol, Water: Methanol (1:1) and Chloroform: Methanol (1:1)]. MTT assay was performed for estimating the cell viability and the results were expressed as % growth inhibition. Overall based on the MTT assay performed on yeast cells, Water: Methanol, Methanol and Water extracts showed higher antiproliferative activity than the positive control (Paclitaxel) in a dose-dependent manner. For Bauhinia variegata and Leptadenia reticulata, methanol extract (25°C temperature & elevated temperature, respectively) exhibited highest inhibition which was approximately 80%. In conclusion, this study has indicated that Bauhinia variegata and Leptadenia reticulata extracts possess strong antiproliferative activity. Furthermore, it could act as a potential source for identifying new molecules with chemotherapeutic potential against cancer.
Keywords:
Medicinal plants, Fission yeast, S. pombe, Antiproliferative activity, MTT Assay.DOI
https://doi.org/10.25004/IJPSDR.2019.110620References
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