Evaluation of Anticonvulsant Activity of Ethanolic Extract of Leaves of Cajanus cajan (L) Millsp. In Rodents
Abstract
Cajanus cajan (L) Millsp. is the perennial plant belongs to family Fabaceae, commonly called as Pigeon pea plant. The presence of phytoconstituents like flavonoids, the flavanone (substituted) from Cajanus cajan (L) Millsp. have in vitro neuroactive property. This flavanone named as pinostrobin helps to inhibit voltage – gated sodium channels. Because of its bioactive phytoconstituents it may act as antiepileptic drug. To avoid problems like ADR herbal plant might be alternative to treat epilepsy. The current study was therefore carried out to evaluate antiepileptic activity of Ethanolic extract of leaves of Cajanus cajan in rodents. The effect of ELECC in MES-induced convulsions in rat and PTZ-induced convulsion in mice was evaluated using doses 100 mg/kg and 200 mg/kg for 7 days. Phenytoin (25 mg/kg), Diazepam (4 mg/kg) was used as standard drug for respective model. Depending on the model, outcome measures were abolishment of Hind Limb Tonic Extensor phase in MES-induced convulsion in rat and onset of latency, recovery or death in PTZ-induced convulsion in mice as well as biochemical estimation of amino acid neurotransmitter (GABA, Glutamate) were evaluated. The ELECC at doses 100 and 200 mg/kg significantly delayed the HLTE phase in MES-induced convulsions in rat whereas, significantly increased onset of latency in PTZ-induced convulsion in mice. It also showed significant (p>0.0001) effect on the level of GABA and Glutamate in dose dependent manner in both models. The phytochemical study of C. cajan showed the presence of Glycosides, Flavonoids, Flavonones, Steroids, Tannins, Fixed oil, Fatty acids and Proteins. As the flavonoids present in C. cajan may contribute to the anticonvulsant activity of the plant. Therefore, the presence of such compounds in the extract may be responsible for the anticonvulsant effect. Therefore, present study validates its anticonvulsant activity. Further, research is required to elucidate its specific mechanism of action and isolation of responsible active principles.
Keywords:
Epilepsy, Antiepileptic drugs, Ethanolic extract, Cajanus cajan (L) Millsp.DOI
https://doi.org/10.25004/IJPSDR.2019.110608References
Bodhankar SL, Vyavhare NS. A Textbook of Pathophysiology. Edn 4. Nirali Prakashan, Pune, 2007. pp. 3.1-3.18.
Shakirullah, NA, Khan A, Nabi M. The Prevalence, Incidence and Etiology of Epilepsy. International Journal of Clinical and Experimental Neurology. 2014; 2(2): 29-39.
Ramani SG, Bajracharya R. Antiepileptic and Anxiolytic Activity of Ethanolic Extract of Brassica nigra L. Koch Seeds on Wistar Albino Rats. European Journal of Pharmaceutical and Medical Research. 2016; 3(4): 394-402.
Herbal remedies used in the treatment of Epilepsy. [Internet] 2019. Available from: <https://www.pharmatutor.org/articles/herbal remedies used in the treatment of epilepsy [Accessed on: 4 Aug 2018]
Mukhopadhyay HK, Kandhar CC, Das SK, Ghosh L, Gupta BK. Epilepsy and its Management: A Review. Journal of Pharmacology. 2012; 1(2): 20-26.
Patil CD, Ahire YS, Pathade DA, Pathade VV, Mali PR. Free radicals, epilepsy and antioxidant: An overview. International Research Journal of Pharmacy. 2011; 2(2): 64-71.
Asmatanzeem B, Parashivamurthy BM, Shaik KN. Evaluation of Anticonvulsant Activity of volatile oil extract of Nigella sativa seeds by chemically induced seizure model in albino rats. International Journal of Basics and Clinical Pharmacology. 2016; 18: 1300 – 1307.
Quintans JLJ, Almeida JRGS, Lima JT, Nunes XP, Siqueira JS, de Oliveira LEG, et al. Plants with anticonvulsant properties - a review. Brazilian Journal of Pharmacognosy 2008; 18 (Supl.):798-819.
Pal D, Mishra P, Sachan N, Ghosh AK. Biological activities and medicinal properties of Cajanus cajan (L) Millsp. Journal of Advanced Pharmaceutical Technology and Research, 2011; 2(4): 207 -213.
Jaliwali YA, Mohanti PK, Jain NK. Antiulcer and Anti-inflammatory Activity of Cajanus cajan Linn. International Research Journal of Pharmacy. 2014; 5(4): 313-316.
Khandelwal KR. Practical Pharmacognosy. 22nd ed. Pune: Nirali Prakashan; 2012. p. 25.1-25.9.
H. Gerhard Vogel (Ed). In: Chapter E, Vogel WH, Scholkens BA, Muller J, Vogel WF (eds.) Drug Discovery & Evaluation. Pharmacological Assays. Ed. 2. New York: Sringer –Verlag; 2002. pp. 487-491.
Kulkarni SK. Handbook of Experimental Pharmacology. Ed. 3. Vallabh Publication, Mumbai, 2011. pp. 131-134.
Kasturi S, Kavimani S, Devi R, Sundharajan R, Deepa N. Anticonvulsant activity of Mahakalyanaka Ghrita in Maximal Electroshock and Pentylenetetrazole induced seizures in rats. International Research Journal of Pharmacy. 2015; 6(10): 715-719.
Rahman Habibur, Eswaraiah MC. [Internet] Available from: https:// www.pharmatutor.org/articles/simple spectroscopic - method - estimating – brain – neurotransmitter – antioxidant – enzymes – lab – animals. (Accessed on: 7 August 2018)
Sancheti JS, Shaik MF, Khatwani PF, Kulkarni SR, Sathaye S. Development and Validation of HPTLC method for simultaneous estimation of L-Glutamic Acid in mice brain. Indian Journal of Pharmaceutical Sciences. 2013; 75(6): 716-721.
Joy Elizabeth A, Thalanjeri P, Manikkoth S. Anticonvulsant activity of Moringa oleifera in swiss albino mice. International Journal of Applied Biology and Pharmaceutical Technology. 2015; 6(2): 140-146.
Myanglambam DM, Leisangthem DT, Nameirak Pam DM, Krishna Khondram DP, Akham DS. Anticonvulsant effect of Portulaca oleracea in experimental animal models. Journal of Medical Society. 2016; 30: 94-97.
Shetty AK, Vinutha N, Kalleshappa CM, Akshata, Krupanidhi AM. Antiepileptic activities of ethanolic extract of leaves of Chromolaenaodarata. IOSR Journal of Engineering. 2017; 7(3): 66-69.
Sankari M, Chitra V, Silambu JP, Raju D. Anticonvulsant activity of ethanolic extract of Aegle marmelos (leaves) in mice. International Journal of PharmTech Research. 2010; 2(1): 640-643.
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