In-vitro Antioxidant, Antimicrobial and Anticancer Potential of Polysaccharide from Tridax procumbens L.
Abstract
Cancer ranks among the primary causes of death on a global level. Natural products are crucial for both cancer therapy and treatment. Traditionally, Tridax procumbens has been used to cure wound infections. The present study investigates the isolation, identification, in-vitro antioxidant, antimicrobial, and anticancer activities of isolated polysaccharides from T. procumbens L. The polysaccharide (4-deoxy-5-α-D-Rhamnonic acid (1→2) β-D-fructofuranosyl (2→1)-β-D-fructofuranosyl (2→1)-2-D-fructofuranoside) was identified using modern NMR spectroscopic techniques. In-vitro anticancer activity was assessed against MDA-MB-249 and MCF-7 using an MTT assay. The IC50 value for polysaccharide was found to be 5.06 μg/mL against MCF-7 and that of MDA MB 249 cell lines 15.68 μg/mL. The statistical analysis indicated significance at P < 0.05. The polysaccharide showed effective antibacterial activity at MIC 15.5 µg/mL against bacteria S. aureus and P. aeruginosa, respectively. The DPPH antioxidant potential of the polysaccharide was found to be outstanding, with an IC50 value of 1.01 µg/mL. It showed statistical significance at P<0.05. Therefore, the findings reveal that polysaccharides could be used as therapeutic drugs to develop anticancer and antibacterial agents from T. procumbens L.
Keywords:
T. procumens L. , Polysaccharide, Anticancer activity, Antioxidant activity, Antbacteriall activityDOI
https://doi.org/10.25004/IJPSDR.2024.160416References
Rahim A, Mostofa MG, Sadik MG, Rahman MA, Khalil MI, Tsukahara T, et al. The anticancer activity of two glycosides from the leaves of Leea aequata L. Nat Prod Res. 2021; 35(24):5867–71. Available from: https://doi.org/10.1080/14786419.2020.1798661
Shah SC, Kayamba V, Peek RM, Heimburger D. Cancer control in low- and middle-income countries: Is it time to consider screening? J Glob Oncol. 2019; (5):1–8. Available from: https://doi.org/10.1200/JGO.18.00200
Malumbres M, Barbacid M. Cell cycle, CDKs and cancer: A changing paradigm. Nat Rev Cancer. 2009; 9(3):153–66. Available from: https://doi.org/10.1038/nrc2602
Song H, Zhang S, Mou J, Gong G, Huang Y, Ma R, et al. Cytotoxic activities against MCF-7 and MDA-MB-231, antioxidant and α-α-glucosidase inhibitory activities of Trachelospermum jasminoides extracts in vitro. Biotechnology & Biotechnological Equipment. 2019; 33(1):1671–9. Available from: https://doi.org/10.1080/13102818.2019.1694436
Newman DJ, Cragg GM. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. J Nat Prod. 2020; 83(3):770–803. Available from: https://doi.org/10.1021/acs.jnatprod.9b01285
Wang J, Zhang Y, Lu Q, Xing D, Zhang R. Exploring carbohydrates for therapeutics: A review on future directions. Front Pharmacol. 2021;16:12. Available from: https://doi.org/10.3389/fphar.2021.756724
Sandhya T, Lathika KM, Pandey BN, Mishra KP. The potential of traditional Ayurvedic formulation, Triphala, as a novel anticancer drug. Cancer Lett. 2006; 1(2):206–14. Available from: https://doi.org/10.1016/j.canlet.2005.01.035
Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathog Glob Health. 2015; 109(7):309-18. doi: 10.1179/2047773215Y.0000000030.
Dhingra S, Rahman NA, Peile E, Rahaman M, Sartelli M, Hassali MA, et al. Microbial resistance movements: An overview of global public health threats posed by antimicrobial resistance, and how best to counter. Front Public Health. 2020; 4:8. Available from: https://doi.org/10.3389/fpubh.2020.535668
Pang G, Wang F, Zang LW. Dose matters direct killing or immunoregulatory effects of natural polysaccharides in cancer treatment. Carbohydr Polym. 2018; 195:243-56. Available from: https://doi.org/10.1016/j.carbpol.2018.04.100
Cao X, Du X, Jiao H, An Q, Chen R, Fang P, et al. Carbohydrates-based drugs launched during 2000-2021. Acta Pharm Sin B. 2022; 12(10):3783-821. Available from: https://doi.org/10.1016/j.apsb.2022.05.020
Zhang H, Shi LE, Zhou J. Recent developments of polysaccharide-based double-network hydrogels. Journal of polymer science. 2023; 61(1):7-43. Available from: https://doi.org/10.1002/pol.20220510
Zhang Y, Wang F. Carbohydrate drugs: current status and development prospect. Drug Discov Ther. 2015; 9(2):79-87. Available from: https://doi.org/10.5582/ddt.2015.01028
Ghosh D, Karmakar P. Insight into antioxidative carbohydrates polymers from medicinal plants: structure-activity relationships, mechanism of actions and interactions with bovine serum albumin. Int J Biol Macromol. 2021; 1:1022-34. Available from: https://doi.org/10.1016/j.ijbiomac.2020.10.258
Kim J-H, Baek J, Sa S, Park J, Kim M, Kim W. Kestose-enriched fructooligosaccharide alleviates atopic dermatitis by modulating the gut microbiome and immune response. J Funct Foods. 2021; 85:104650. Available from: Available from: https://doi.org/10.1016/j.jff.2021.104650
Li Y, Guo X, Zhong R, Ye C, Chen J. Structure and characterization and biological activities evaluation of two hetero-polysaccharides from Lepista nuda: Cell antioxidants, anticancer and immune-modulatory activities. Int J Biol Macromol. 2023; 31:244:125204. Available from: https://doi.org/10.1016/j.ijbiomac.2023.125204
Udupa S, Udupa A, Kulkarni D. Influence of Tridax procumbens on lysyl oxidase activity and wound healing. Plant Med. 1991; 57(04):325-7. Available from: https://doi.org/10.1055/s-2006-960108
Ingole VV, Mhaske PC, Katade SR. Phytochemistry and pharmacological aspects of Tridax procumbens (L): A systematic and comprehensive review. Phytomedicine plus. 2022; 2(1):100199. Available from: https://doi.org/10.1016/j.phyplu.2021.100199
Ali M, Ravinder E, Ramachandram R. A new flavonoid from the aerial parts of Tridax procumbens. Fitoterapia. 2001; 72(3):313-5. Available from: https://doi.org/10.1016/S0367-326X(00)00296-3
Cui HX, Zhang LS, Yan HG, Yuan K, Jin SH. Constituents of flavonoids from Tridax procumbens L. and antioxidant activity. Pharmacogn Mag. 2020; 16(67):201-5. Available from: Available from: https://doi.org/10.4103/pm.pm_229_19
Dawood DH, Elmongy MS, Negm A, Taher MA. Extraction and chemical characterization of water-soluble polysaccharides from two palm species and their antioxidants and antitumor activities. Egyptian Journal of Basic and Applied Sciences. 2020; 7(1):141-58. Available from: https://doi.org/10.1080/2314808X.2020.1773126
Eloff J. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998; 4:711-3. Available from: Available from: https://doi.org/10.1055/s-2006-957563
Sarkar SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007; 42(4):321-4. https://doi.org/10.1016/j.ymeth.2007.01.006
Ingole VV, Katade SR. Chemical composition, antioxidant, antibacterial activity of isolated oil and methanol extract of Tridax procumbens L. Int J Pharm Sci Res. 2024; 15(4):1157-66. Available from: https://doi.org/10.13040/IJPSR.0975-8232
Brand- Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology. 1995; 28(1):25-30. Available from: https://doi.org/10.1016/S0023-6438(95)80008-5
Xu M, McCanna DJ, Sivak JG. Use of the viability regent presto blue in comparison with Alamar blue and MTT to assess the viability of human corneal epithelial cells. J Pharmacol Toxicol Methods. 2015; 71:1-7. Available from https://doi.org/10.1016/j.vascn.2014.11.003
Hong T, Yin JY, Nie SP, Xie MY. Applications of infrared spectroscopy in polysaccharide structural analysis: Progress, challenge and perspective. Food Chem X. 2021; 100168. Available from: https://doi.org/10.1016/j.fochx.2021.100168
De Bruyan A, Van LJ. The identification by 1H-and 13C-n.m.r. spectroscopy of sucrose,1-ketose, and neokestose in mixtures present in plant extracts. Carbohydr Res. 1991; 2: 131-6. Available from: https://doi.org/10.1016/0008-6215(91)84151-4
Le TH, Le LS, Nguyen DC, Tran TT, Vu Ho XA, Tran TM, et al. Rich d-fructose-containing polysaccharide isolated from Myxopyrum smilacifolium roots toward a superior antioxidant biomaterial. ACS Omega. 2022; 27:47923–32. Available from: https://doi.org/10.1021/acsomega.2c05779
Jeevitha J, Ramanan R. The efficiency of phytobiotics of Indian medicinal plant Tridax procumbens L. against wound infecting bacteria. MOJ Curr. Res. Rev. 2018; 1:278-80.
Huong PT, Trang DT, Thu VK, Mai NT, Nhiem NX, Yen PH, et al. Four new triterpene glycosides from the aerial parts of chenopodium album and their cytotoxic activity. Phytochem Lett. 2021; 44:7–13. Available from: https://doi.org/10.1016/j.phytol.2021.05.004
Choucry MA, Shalabi AA, El Halawany AM, El-Sakhawy FS, Zaiter A, Morita H, et al. New pregnane glycosides isolated from Caralluma hexagonal lavranos as inhibitors of α-Glucosidase, pancreatic lipase, and advanced glycation end-product formation. ACS Omega. 2021; 27:18881–9. Available from: https://doi.org/10.1021/acsomega.1c02056
Jeevitha J, Ramanan R. The efficiency of phytobiotics of Indian medicinal plant Tridax procumbens L. against wound infecting bacteria. MOJ Curr. Res. Rev. 2018; 1:278-80.
Syed A, Benit N, Alyousef AA, Alqasim A, Arshad M. In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of Tridax procumbens. Saudi journal of biological sciences. 2020; 1:757-61. https://doi.org/10.1016/j.sjbs.2019.12.031
Fischer D, Geyer A, Loos E. Occurrence of glucosyl sucrose [α‐D‐glucopyranosyl‐(1→ 2)‐α‐D‐glucopyranosyl‐(1→ 2)‐β‐D‐fructofuranoside] and glucosylated homologues in cyanobacteria: structural properties, cellular contents and possible function as thermoprotectants. FEBS J. 2006; 13:137–49. Available from: https://doi.org/10.1111/j.1742-4658.2005.05050.x
Petraglia T, Latronico T, Fanigliulo A, Crescenzi A, Liuzzi GM, Rossano R. Antioxidant activity of polysaccharides from the edible mushroom Pleurotus eryngii. Molecules. 2023; 26:2176. Available from: https://doi.org/10.3390/molecules28052176
Bush PL. pectin: chemical properties, uses and health benefits. Nova Science Publishers, Incorporated. 2014, 1-288.
Delphi L, Sepehri H, Khorramizadeh MR, Mansoori F. Pectic-oligosaccharides from apples induce apoptosis and cell cycle arrest in MDA-MB-231 cells, a model of human breast cancer. Asian Pacific Journal of Cancer Prevention. 2015; 3:5265-71. Available from: https://doi.org/10.7314/APJCP.2015.16.13.5265
Published


How to Cite
Issue
Section
Copyright (c) 2024 Varsharani V Ingole, Pravin C Mhaske, Sushma R Katade

This work is licensed under a Creative Commons Attribution 4.0 International License.