Anti-ulcer activity of Trianthema portulacastrum leaves in rats using an experimental ulcer model
Abstract
Gastric ulcers conditions can lead to severe complications, including bleeding, perforation, and an increased risk of gastric carcinoma. Natural remedies such as Trianthema portulacastrum may help alleviate these issues by boosting antioxidant defenses and reducing inflammation. In this experiment, thirty Wistar Albino rats were assigned to different groups and observed for 16 days. The study design included several treatment approaches for gastric ulcers. The normal control (Untreated) group was received normal saline for 15 days, while the disease control group also received normal saline for the same period. The Standard Treatment group was received Omeprazole (20 mg/kg) over the 15-day duration. The first test drug group received a daily dose of 250 mg/kg of Trianthema portulacastrum extract for 15 days, while the second Test Group received 500 mg/kg of the extract for the same period. All groups, except the normal control, were administered ethanol (1 mL/200 g, p.o.) on the 16th day. Multiple variables including ulcer index, percentage inhibition of ulceration, antioxidant markers (SOD and CAT), and histopathological analysis of the stomachs of treated rats, were assessed. The methanolic extract of Trianthema portulacastrum (METP) demonstrated potential in treating ulcers. In an ethanol-induced ulcer model, the METP increased SOD and CAT levels, essential antioxidant enzymes that neutralize reactive oxygen species, indicating its capacity to alleviate oxidative stress and inflammation. Notably, the higher METP dose (500 mg/kg) significantly elevated SOD levels, approaching those observed in the normal control group. Both the standard drug Omeprazole and METP markedly reduced ulcer severity, count, and index, while also increasing the percentage of inhibition. The METP exhibited dose-dependent anti-ulcer effects, with the 500 mg/kg dose providing substantial protection against ethanol-induced ulcers. Histopathological studies further supported that Trianthema portulacastrum extracts improve mucosal integrity and reduce lesions. Overall, Trianthema portulacastrum shows promising potential for treating gastric ulcers and enhancing overall health.
Keywords:
Anti-Oxidant Enzyme, Ethanol, Omeprazole, Trianthema portulacastrum and UlcersDOI
https://doi.org/10.25004/IJPSDR.2024.160606References
Yadav P, Ganeshpurkar A, Rai G. In vitro H+ -K+ ATPase inhibitory potential of methanolic extract of Cissus quadrangularis Linn. Phcog. 2012;4(2):123-6.
Ramakrishnan K, Salinas RC. Peptic ulcer disease. Am Fam Physician. 2007;76(7):1005-12. Available from:
https://www.aafp.org/afp/2007/1001/p1005.html
Narayanan M, Reddy KM, Marsicano E. Peptic ulcer disease and Helicobacter pylori infection. Mo Med. 2018 May-Jun;115(3):219-24.
Lanas Á, Carrera-Lasfuentes P, Arguedas Y, García S, Bujanda L, Calvet X, et al. Risk of upper and lower gastrointestinal bleeding in patients taking nonsteroidal anti-inflammatory drugs, antiplatelet agents, or anticoagulants. Clin Gastroenterol Hepatol. 2015 May;13(5):906-12.e2.
Available from: https://doi.org/10.1016/j.cgh.2014.11.007
Huang JQ, Sridhar S, Hunt RH. Role of Helicobacter pylori infection and non-steroidal anti-inflammatory drugs in peptic-ulcer disease: a meta-analysis. Lancet. 2002 Jan 05;359(9300):14-22. Available from: https://doi.org/10.1016/S0140-6736(02)07273-2
Kumar K, De AK. Phytochemical and pharmacological properties of Trianthema portulacastrum: A review. J Pharmacogn Phytochem. 2017;6(1):100-4.
Sasidharan S, Gopinath S. Anti-inflammatory and antioxidant properties of Trianthema portulacastrum. J Med Plants Res. 2011;5(14):3353-60.
Mahapatra S, Pati S. Antimicrobial activity of Trianthema portulacastrum leaves: An in vitro study. Asian J Plant Sci. 2012;11(2):101-6.
Sarker SD, Nahar L. Bioactive natural products from Trianthema portulacastrum: An update. Nat Prod Res. 2016;30(18):2093-104. Available from: https://doi.org/10.1080/14786419.2016.1148186
Rajasekaran S, Karthikeyan R. Diuretic and antioxidant effects of Trianthema portulacastrum in experimental rats. Pharmacogn Res. 2013;5(4):227-32.
Jha DK, Koneri R, Samaddar S. Toxicity studies of a saponin isolated from the fruits of Momordica dioica in rats. Int J Pharm Sci Res. 2019;10(10):4462-76. Available from: doi.org/10.13040/IJPSR.0975-8232
Jha DK, Koneri R, Samaddar S. Antidiabetic activity of phytosaponin in STZ-induced Type I diabetes in rats. Res J Pharm Technol. 2019;12(8):3919-26. Available from: doi.org/10.5958/0974-360X.2019.00675.9
Abebaw M, Mishra B, Asmelashe D, Gelayee. Evaluation of anti-ulcer activity of the leaf extract of Osyris quadripartita Decne. (Santalaceae) in rats. J Exp Pharmacol. 2017;9:1-11. Available from: https://doi.org/10.2147/JEP.S138734
Khairullah MN, Jameel QY, Ajeel MA. Nutritional and anti-gastro ulcerative role of the gum Arabic (Acacia senegal L.) compared to a reference drug. Funct Foods Health Dis. 2022;12(6):294-7.
Karuna R, Sreenivasa RS, Baskar R, Saralakumari D. Antioxidant potential of aqueous extract of Phyllanthus amarus in rats. Indian J Pharmacol. 2009;41(2):64-7.
Raju D, Ilango K, Chitra V, Ashish K. Evaluation of anti-ulcer activity of methanolic extract of Terminalia chebula fruits in experimental rats. J Pharm Sci Res. 2009;1(3):101-7.
Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol. 2011;3(12):10-4.
Fennell CW, Lindsey KL, McGaw LJ, Sparg SG, Stafford GI, Elgorashi EE, et al. Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. J Ethnopharmacol. 2004;94(2-3):205-17. Available from: https://doi.org/10.1016/j.jep.2004.05.001
Tan PV, Nyasse B. Anti-ulcer compound from Voacanga africana with possible histamine H2 receptor blocking activity. Phytomedicine. 2000;7:509-15. Available from: https://doi.org/10.1016/S0944-7113(00)80080-4
Maity B, Chattopadhyay S. Natural antiulcerogenic agents: An overview. Curr Bioact Compd. 2008;4:225-44.
Thirunavukkaras P, Ramkumar L, Ramanathan T. Anti-ulcer activity of Excoecaria agallocha bark on NSAID-induced gastric ulcer in Albino rats. Glob J Pharmacol. 2009;3(3):123-6.
Awaad S, El-Meligy RM, Soliman GA. Natural products in treatment of ulcerative colitis and peptic ulcer. J Saudi Chem Soc. 2013;17(1):101-24. Available from: https://doi.org/10.1016/j.jscs.2012.03.002
Adinortey MB, Ansah C, Galyuon I, Nyarko A. In vivo models used for evaluation of potential antigastroduodenal ulcer agents. Ulcers. 2013.
Published
Abstract Display: 87 
PDF Downloads: 77 How to Cite
Issue
Section
Copyright (c) 2024 Puja Bharti, U Rajashekhar, Deepak Kumar Jha
This work is licensed under a Creative Commons Attribution 4.0 International License.
