The Effect on skin papillomas by administration of Fruits of Momordica dioica extract in DMBA/Croton oil induced benign cancer in mice

Authors

  • Amani Abdalbagi Eshag Hassan Department of Pharmacology, Karnataka College of Pharmacy, Bangalore, Karnataka, India
  • Deepak Kumar Jha Department of Pharmacology, Karnataka College of Pharmacy, Bangalore, Karnataka, India https://orcid.org/0000-0002-1979-7940

Abstract

Skin papillomas are not cancerous, but they can trigger an inflammatory disorder and irritate the area where the wart is present. The study was to find natural agents that prevent and/or cure skin papillomas. The fruits of Momordica dioica have been known to contain potent phytoconstituents that have anti-inflammatory, cardiovascular, diabetic, oncological, and other health benefits. To investigate inhibition of skin papilloma’s by administration of a fruit extract of Momordica dioica in DMBA/Croton oil-induced benign cancer in mice. Induction of papilloma’s by topical application of DMBA/Croton oil for 4-5 weeks; after confirmation of papilloma's, the Momordica dioica and 5-Flu10 mg/kg were administered. Body weight, tumor incidence, cumulative number of tumors, tumor yield, average latency time period, number of papillomas, hematological parameters, antioxidant enzymes, and pro-inflammatory cytokines were measured. 100% tumor incidence was observed in DMBA/croton oil, whereas the other groups except the normal control had quite a similar tumor incidence. The cumulative number, tumor yield, and average latent period have significantly improved with the treatment in skin papilloma mice. Hematological markers and NLR (< 3.0) have improved (p<0.001), which is indicative of reduced acute systemic inflammation. The deviations on LPO and CAT from baselines were corrected by Momordica dioica and found to have a significant impact on their levels (p<0.001). Post-treatment of immunosuppressive markers such as; TGF-beta (p<0.001), TNF-alpha (p<0.001), and IL-6 (p<0.001) were decreased significantly and increased the levels of IFG-g (p<0.001), which correlates with increased immune activation. Hence, the intake of fruits of Momordica dioica inhibits the growth of skin papillomas.

Keywords:

Skin cancer, DMBA/Croton oil, Papillomas, Pro-Inflammatory cytokines, Anti-oxidants Enzyme and Momordica dioica

DOI

https://doi.org/10.25004/IJPSDR.2024.160109

References

https://www.medicalnewstoday.com/articles/323648.

https://www.emedicinehealth.com.

Sabry AO, Patel BC. Papilloma. In: StatPearls. Treasure Island (FL): StatPearls Publishing 2023. https://www.ncbi.nlm.nih.gov/books/NBK560737/.

Radvanyi LG. Tumor-Infiltrating Lymphocyte Therapy: Addressing Prevailing Questions. Cancer journal. 2015; 21:450–464.

Bajin Wei, Minya Yao, Chunyang Xing, Wei Wang, Jia Yao, Yun Hong, Yu Liu Peifen Fu. The neutrophil lymphocyte ratio is associated with breast cancer prognosis: an updated systematic review and meta-analysis. OncoTargets and Therapy. 2016; 9:5567–5575.

Grivennikov SI, Greten FR, Karin M. Immunity, Inflammation, and Cancer, Cell. 2010; 140(6):883–899.

Leanna J. Standish et al, Immune Defects in Breast Cancer Patients after Radiotherapy J Soc Integr Oncol. 2008; 6(3):110–121.

Brett Burkholder A et al. Tumor-induced perturbations of cytokines and immune cell networks Biochimica et Biophysica Acta. 2014; 1845:182–201.

Yu H, Pardoll D, Jove R. STATs in cancer inflammation and immunity: a leading role for STAT3 Nat Rev Cancer. 2009; 9(11):798–809.

Sung Hsieh HH, Chung MT, Allen RM, Ranganathan K, Habbouche J, Cholok D et al. Evaluation of Salivary Cytokines for Diagnosis of both Trauma-Induced and Genetic Heterotopic Ossification. Front. Endocrinol. 2017; 8:74.

Brown JP. A review of the genetic effect of occurring flavonoids, Anthraquinones and related compounds. Mutat Res. 1980; 75:243-77.

Davies J. Inactivation of antibiotics and the dissemination of resistance genes. Science Direct. 1994; 264:375-82.

Sharmila R, Manoharan S. Anti-tumor activity of rosmarinic acid in 7,12- dimethylbenz(a)anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice. Indian J Exp Biol. 2012; 50(3):187-94.

Das I, Das S, Saha T. Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: A histopathological study. Acta Histochem. 2010; 112(4):317-27.

Prakash J, Gupta SK, Dinda AK. Withania somnifera root extract prevents DMBA induced squamous cell carcinoma of skin in Swiss albino mice. Nutr Cancer. 2002; 42(1):91-7.

Das I et al. Effect of garlic on lipid peroxidation and antioxidation enzymes in DMBA-induced skin carcinoma. Nutrition. 2009; 25(4):459-471.

Haimed YAS, Sharma PK, Jha DK, Sharma J. A Comparative Study of Polyherbal Plants for the Management of Streptozotocin-Induced Diabetes in Rats. Lat. Am. J. Pharm. 2023; 42(3):1257-70.

Jha DK, Koneri R and Samaddar S: Toxicity studies of a saponin isolated from the fruits of Momordica dioica in rats. Int J Pharm Sci & Res. 2019; 10(10):4462-76.

Elyasi Z, Jha DK, Rajashekhar U, Khadka J. Evaluation of In-vivo Anticancer Activity Choerospondias axillaris in Swiss Albino Mice Models. JPRI. 2022; 34(54A):26-50.

Bashir A, Asif M, Saadullah M et al: Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies. ACS Omega. 2022; 7:25772-82.

Marie MC, Demeule M et al. Curcumin inhibits tumor growth and angiogenesis in glioblastoma xenografts. Molecular Nutrition & Food Research. 2010; 54(8):1192-1201.

Akram M, Uddin S et al. Curcuma Longa A Curcumin: A Review Article. Rom. J. Biol. Plant Biol. 2010; 55(2):65-70.

Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1997; 95:351–358.

Jha DK, Koner R, Samaddar S. Optimization of pancreatic islet isolation from rat and evaluation of islet protective potential of a Phytosaponin. JAPS. 2020;10(07):89-99.

Aebi H. Catalase. In Methods of Enzymatic Analysis. 2nd edn. Hans Ulhrich Bergmeyer, Verlag Chemie Int., FL. 1947; 2:673–682.

Alshargabi MG, Jha DK. Pharmacological Evaluation of Methanolic Extraction of Fruits of Momordica Dioica on L-Arginine Induced Pancreatitis In Rats. JETIR. 2021; 8(8):170-80.

Haimed Y, Sharma PK, Jha DK. An investigational on the Type I anti-diabetic activity of the methanolic extract of Moringa oleifera in streptozotocin induced rat model. J. Bio. Innov. 2022; 11(1):74-91.

Mohan CV, Jha DK, Chatterjee S. Pharmacological Evaluation of Moringa oleifera on Collagen- Induced Arthritis in Rats. JPRI. 2022; 34(54B):11-32.

Jha DK, Koner R, Samaddar S. Toxicity studies of a saponin isolated from the fruits of Momordica dioica in rats. Int. J. Pharm. Sci. & Res. 2019; 10(10):1000-15.

Bhutia YD, Saini M, Sharma AK, Sharma B, Swarup D. Efficacy of Curcuma longa extract against DMBA induced skin cancer in rats. Journal of Applied Animal Research. 2009; 36(2):291-296.

Lippitz, Bodo E, and Robert A. Harris. Cytokine Patterns in Cancer Patients: A Review of the Correlation between Interleukin 6 and Prognosis. Oncoimmunology. 2016; 5(5):e1093722.

Jing S, Du J, Yang J, Hu X, Li L. Changes of Serum Levels of Tumor Necrosis Factor (TNF-α) and Soluble Interleukin-2 Receptor (SIL 2R) in Patients with Cervical Cancer and Their Clinical Significance. American Journal of Translational Research. 2021; 13(6):6599– 6604.

Parashar, Dattatreyudu Nori KS, Chao C, Wernicke AG. Transforming Growth Factor β-1 (TGF-Β1) is a Serum Biomarker of Radiation Induced Fibrosis in Patients Treated with Intracavitary Accelerated Partial Breast Irradiation: Preliminary Results of a Prospective Study. International Journal of Radiation Oncology, Biology, Physics. 2013; 87(5):1030–36.

Diego KG, Kahnert K, Kiefl R, Sellmer L, Walter J, Behr J, Tufman A. Systemic Inflammation and Pro-Inflammatory Cytokine Profile Predict Response to Checkpoint Inhibitor Treatment in NSCLC: A Prospective Study. Scientific Reports. 2021; 11(1):10919.

Published

30-01-2024
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How to Cite

“The Effect on Skin Papillomas by Administration of Fruits of Momordica Dioica Extract in DMBA Croton Oil Induced Benign Cancer in Mice”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 16, no. 1, Jan. 2024, pp. 59-66, https://doi.org/10.25004/IJPSDR.2024.160109.

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Research Article

How to Cite

“The Effect on Skin Papillomas by Administration of Fruits of Momordica Dioica Extract in DMBA Croton Oil Induced Benign Cancer in Mice”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 16, no. 1, Jan. 2024, pp. 59-66, https://doi.org/10.25004/IJPSDR.2024.160109.

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