A PROMISING TECHNIQUE FOR RAPID SCREENING AND CONFIRMATION OF L-GLUTAMINASE - A TUMOUR INHIBITOR FROM NOVEL PENCILLIUM EXPANSUM

Authors

  • Sunil Dutt P L N S N Research and Development centre Bharathiar University, Coimbatore, Tamil Nadu, India
  • Siddalingeshwara K. Gurubasappa Department of Microbiology and Biochemistry, Padmashree Institute of Information Sciences, Nagarabhavi Circle, Bangalore, Karnataka, India
  • Sudipta K. Mohanty Department of Microbiology and Biochemistry, Padmashree Institute of Information Sciences, Nagarabhavi Circle, Bangalore, Karnataka, India
  • Karthic J. Research and Development centre Bharathiar University, Coimbatore, Tamil Nadu, India
  • Shantaveer G. Research and Development centre Bharathiar University, Coimbatore, Tamil Nadu, India
  • Vishwanatha T. Department of Studies in Microbiology, Maharani Science College Women’s, Bangalore, Karnataka, India
  • Mohsin S. Mushtaq Research and Development centre Bharathiar University, Coimbatore, Tamil Nadu, India
  • Dhulappa A. Department of Studies in Microbiology, Maharani Science College Women’s, Bangalore, Karnataka, India
  • Dayananda K. S. Department of Microbiology and Biochemistry, Padmashree Institute of Information Sciences, Nagarabhavi Circle, Bangalore, Karnataka, India

Abstract

All fourteen isolates of Pencillium expansum were screened for the production of L-glutaminase. The screening of L-glutaminase producing isolates carried out by using modified Czapek Dox’s agar plate. Out of fourteen isolates the strain P. expansum KGSSD 08 were showed high and potential L-glutaminase producer. It showed maximum 1.11cm zone of diameter. Then the rapid confirmation of L-glutaminase producing Pencillium expansum KGSSD 08 were carried out by thin layer chromatography and the Rf Values were determined. The Rf value is 0.268.This Rf was close to that of standard glutamic acid.

Keywords:

L-glutaminase, plate assay, Pencillium expansum, thin layer chromatography

DOI

https://doi.org/10.25004/IJPSDR.2010.020410

References

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Published

01-10-2010
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How to Cite

“A PROMISING TECHNIQUE FOR RAPID SCREENING AND CONFIRMATION OF L-GLUTAMINASE - A TUMOUR INHIBITOR FROM NOVEL PENCILLIUM EXPANSUM”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 2, no. 4, Oct. 2010, pp. 275-7, https://doi.org/10.25004/IJPSDR.2010.020410.

Issue

Volume 2, Issue 4, 2010

Section

Research Article

How to Cite

“A PROMISING TECHNIQUE FOR RAPID SCREENING AND CONFIRMATION OF L-GLUTAMINASE - A TUMOUR INHIBITOR FROM NOVEL PENCILLIUM EXPANSUM”. International Journal of Pharmaceutical Sciences and Drug Research, vol. 2, no. 4, Oct. 2010, pp. 275-7, https://doi.org/10.25004/IJPSDR.2010.020410.